This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Invitrogen
other brands :
NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience
product type :
antibody
product name :
6x-His Tag Recombinant Superclonal Antibody (21HCLC)
catalog :
710286
quantity :
100 ug
price :
US 458.00
clonality :
monoclonal
host :
domestic rabbit
conjugate :
nonconjugated
clone name :
21HCLC
application :
western blot, immunocytochemistry
citations: 3
Reference
Chatterjee A, Cerna Sanchez J, Yamauchi T, Taupin V, Couvrette J, Gorodetsky A. Cephalopod-inspired optical engineering of human cells. Nat Commun. 2020;11:2708 pubmed publisher
Li Y, Duah E, Long N, Persaud A, Vangundy Z, MAGLIERY T, et al. An efficient and quantitative assay for epitope-tagged therapeutic protein development with a capillary western system. Bioanalysis. 2019;11:471-483 pubmed publisher
Kong B, Moon S, Kim Y, Heo P, Jung Y, Yu S, et al. Virucidal nano-perforator of viral membrane trapping viral RNAs in the endosome. Nat Commun. 2019;10:185 pubmed publisher
product information
Product Type :
Antibody
Product Name :
6x-His Tag Recombinant Superclonal Antibody (21HCLC)
Catalog # :
710286
Quantity :
100 ug
Price :
US 458.00
Clonality :
Recombinant Superclonal
Purity :
protein A
Host :
Rabbit
Reactivity :
Tag
Applications :
Immunocytochemistry: 1:100-1:500, Western Blot: 1-3 ug/mL
Species :
Tag
Clone :
21HCLC
Isotype :
IgG
Storage :
Store at 4 C short term. For long term storage, store at -20 C, avoiding freeze/thaw cycles.
Description :
The 6x His tag is a synthetic oligo peptide consisting of 6 consecutive histidine residues (HHHHHH). The His tag is commonly expressed as a tag at either N-or C-terminal regions of recombinant proteins to allow isolation or purification by immobilized metal affinity chromatography. Epitope tagging is a technique in which a known epitope is fused to a recombinant protein using genetic engineering. By choosing a particular epitope and recombinant protein combination, epitope tagging makes it possible to detect proteins for which no antibody is available. His epitope tagged proteins that contain a stretch of histidine residues at the carboxyl or amino terminus are used for purification of recombinant proteins by means of metal chelate chromatography due to metal binding properties of histidine clusters on His tagged proteins. For example, the affinity of the histidine-tag motif to Ni2+ by chelation is strong and selective enough to enable purification of the protein to homogeneity by affinity chromatography on a Ni2+-NTA adsorbent. His tag-specific antibodies are used to facilitate detection or coimmunopreciptation of tagged proteins.
Immunogen :
Synthetic construct corresponding to amino acids HHHHHH
Format :
Liquid
Applications w/Dilutions :
Immunocytochemistry: 1:100-1:500, Western Blot: 1-3 ug/mL
Aliases :
6His tag; 6X His; His Tag; Histidine Tag
company information
Invitrogen
Thermo Fisher Scientific
81 Wyman Street
Waltham, MA USA 02451
https://www.thermofisher.com
800-678-5599
headquarters: USA