Occludin Recombinant Superclonal Antibody (6HCLC) | Invitrogen 710192 product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

Occludin Recombinant Superclonal Antibody (6HCLC)

catalog :

710192

quantity :

100 µg

price :

US 446

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

6HCLC

reactivity :

human, mouse

application :

western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

more info or order :

Invitrogen product webpage

citations: 8

Published Application/Species/Sample/Dilution	Reference
western blot; mouse; 1:500-1:1000; loading ...; fig 3	Suzuki S, Tanaka A, Nakamura H, Murayama T. Knockout of Ceramide Kinase Aggravates Pathological and Lethal Responses in Mice with Experimental Colitis. Biol Pharm Bull. 2018;41:797-805 pubmed publisher
western blot; human; fig s1	Xiong J, Zhou M, Wang Y, Chen L, Xu W, Wang Y, et al. Protein Kinase D2 Protects against Acute Colitis Induced by Dextran Sulfate Sodium in Mice. Sci Rep. 2016;6:34079 pubmed publisher
western blot; human; 1:1000; loading ...; fig 5a	Kuan W, Bennett N, He X, Skepper J, Martynyuk N, Wijeyekoon R, et al. ?-Synuclein pre-formed fibrils impair tight junction protein expression without affecting cerebral endothelial cell function. Exp Neurol. 2016;285:72-81 pubmed publisher
western blot; mouse; 1:1000; fig 3	Wang X, Fan F, Cao Q. Modified Pulsatilla decoction attenuates oxazolone-induced colitis in mice through suppression of inflammation and epithelial barrier disruption. Mol Med Rep. 2016;14:1173-9 pubmed publisher
	Pepe G, Fioriniello S, Marracino F, Capocci L, Maglione V, D Esposito M, et al. Blood-Brain Barrier Integrity Is Perturbed in a Mecp2-Null Mouse Model of Rett Syndrome. Biomolecules. 2023;13: pubmed publisher
	Hering H, Zoschke C, Kühn M, Gadicherla A, Weindl G, Luch A, et al. TatS: a novel in vitro tattooed human skin model for improved pigment toxicology research. Arch Toxicol. 2020;94:2423-2434 pubmed publisher
	Tang J, Kang Y, Huang L, Wu L, Peng Y. TIMP1 preserves the blood-brain barrier through interacting with CD63/integrin β 1 complex and regulating downstream FAK/RhoA signaling. Acta Pharm Sin B. 2020;10:987-1003 pubmed publisher
	Di Pardo A, Castaldo S, Amico E, Pepe G, Marracino F, Capocci L, et al. Stimulation of S1PR5 with A-971432, a selective agonist, preserves blood-brain barrier integrity and exerts therapeutic effect in an animal model of Huntington's disease. Hum Mol Genet. 2018;27:2490-2501 pubmed publisher

product information

Product Type :

Antibody

Product Name :

Occludin Recombinant Superclonal Antibody (6HCLC)

Catalog # :

710192

Quantity :

100 µg

Price :

US 446

Clonality :

Recombinant Superclonal

Purity :

protein A

Host :

Rabbit

Reactivity :

Human, Mouse

Applications :

Immunocytochemistry: 1:100-1:1,000, Immunohistochemistry (Paraffin): 1:10-1:100, Western Blot: 1:500-1:5,000

Species :

Human, Mouse

Clone :

6HCLC

Isotype :

IgG

Storage :

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Description :

Occludin is a 65 kDa protein that can exist in a variety of phosphorylated forms, ranging up to approximately 82 kDa. Occludin is thought to be involved in regulating both the localization and the function of occludin. Polyunsaturated fatty acids are known to up-regulate occludin expression, increasing the transendothelial cell resistance and reducing the cellular permeability to large molecules. The level of occludin varies greatly depending on tissue; in brain tissue, occludin is highly and continuously expressed at cell-cell contact sites, whereas non-neural tissues show lower expression and discontinuous distribution. Overall structural features of the occludin protein are highly conserved in all the species examined. Under-expression of tight junction proteins, including occludin, is a key molecular abnormality responsible for the increased permeability of tumor endothelial tight junctions, which contributes to brain tumor edemas.

Immunogen :

Peptide corresponding to amino acids 387-403 of human occuludin

Format :

Liquid

Applications w/Dilutions :

Immunocytochemistry: 1:100-1:1,000, Immunohistochemistry (Paraffin): 1:10-1:100, Western Blot: 1:500-1:5,000

Aliases :

AI503564; BLCPMG; Occludin; occludin 1B; Ocl; Ocln; phosphatase 1, regulatory subunit 115; PPP1R115; tight junction protein occludin; tight junction structural protein

more info or order :

Invitrogen product webpage