Phospho-p27 Kip1 (Thr187) Polyclonal Antibody | Invitrogen 71-7700 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

Phospho-p27 Kip1 (Thr187) Polyclonal Antibody

catalog :

71-7700

quantity :

100µg

price :

US 309.00

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

antigen modification :

phosphorylated

reactivity :

human

application :

western blot, ELISA, immunocytochemistry, immunoprecipitation

citations: 11

Published Application/Species/Sample/Dilution	Reference
western blot; human; fig 3b	Yu D, Makkar G, Dong T, Strickland D, Sarkar R, Monahan T. MARCKS Signaling Differentially Regulates Vascular Smooth Muscle and Endothelial Cell Proliferation through a KIS-, p27kip1- Dependent Mechanism. PLoS ONE. 2015;10:e0141397 pubmed publisher
western blot; human; 1:500-1:1000	Lupino E, Ramondetti C, BuccinnÃ B, Piccinini M. Exposure of neuroblastoma cell lines to imatinib results in the upregulation of the CDK inhibitor p27(KIP1) as a consequence of c-Abl inhibition. Biochem Pharmacol. 2014;92:235-50 pubmed publisher
western blot; human	Caraballo J, Acosta J, Cortés M, Albajar M, Gomez Casares M, Batlle López A, et al. High p27 protein levels in chronic lymphocytic leukemia are associated to low Myc and Skp2 expression, confer resistance to apoptosis and antagonize Myc effects on cell cycle. Oncotarget. 2014;5:4694-708 pubmed
western blot; human; fig 7	Bretones G, Acosta J, Caraballo J, Ferrandiz N, Gomez Casares M, Albajar M, et al. SKP2 oncogene is a direct MYC target gene and MYC down-regulates p27(KIP1) through SKP2 in human leukemia cells. J Biol Chem. 2011;286:9815-25 pubmed publisher
western blot; human; 1:200	Wang Y, Fei M, Cheng C, Zhang D, Lu J, He S, et al. Jun activation domain-binding protein 1 negatively regulate p27 kip1 in non-Hodgkin's lymphomas. Cancer Biol Ther. 2008;7:460-7 pubmed
western blot; human; 1:500	Wang Z, Yu B, Rahman K, Ahmad F, Sarkar F. Induction of growth arrest and apoptosis in human breast cancer cells by 3,3-diindolylmethane is associated with induction and nuclear localization of p27kip. Mol Cancer Ther. 2008;7:341-9 pubmed publisher
	Lee J, Song J, Smith R, Kay E. Human corneal endothelial cells employ phosphorylation of p27(Kip1) at both Ser10 and Thr187 sites for FGF-2-mediated cell proliferation via PI 3-kinase. Invest Ophthalmol Vis Sci. 2011;52:8216-23 pubmed publisher
	Xu S, Abbasian M, Patel P, Jensen Pergakes K, Lombardo C, Cathers B, et al. Substrate recognition and ubiquitination of SCFSkp2/Cks1 ubiquitin-protein isopeptide ligase. J Biol Chem. 2007;282:15462-70 pubmed
	Seo D, Li H, Qu C, Oh J, Kim Y, Diaz T, et al. Shp-1 mediates the antiproliferative activity of tissue inhibitor of metalloproteinase-2 in human microvascular endothelial cells. J Biol Chem. 2006;281:3711-21 pubmed
	Zancai P, Dal Col J, Piccinin S, Guidoboni M, Cariati R, Rizzo S, et al. Retinoic acid stabilizes p27Kip1 in EBV-immortalized lymphoblastoid B cell lines through enhanced proteasome-dependent degradation of the p45Skp2 and Cks1 proteins. Oncogene. 2005;24:2483-94 pubmed
	Zhang Y, Nakayama K, Nakayama K, Morita I. A novel route for connexin 43 to inhibit cell proliferation: negative regulation of S-phase kinase-associated protein (Skp 2). Cancer Res. 2003;63:1623-30 pubmed

product information

Product Type :

Antibody

Product Name :

Phospho-p27 Kip1 (Thr187) Polyclonal Antibody

Catalog # :

71-7700

Quantity :

100µg

Price :

US 309.00

Clonality :

Polyclonal

Purity :

Antigen affinity chromatography

Host :

Rabbit

Reactivity :

Human

Applications :

ELISA: Assay Dependent, Immunofluorescence: Assay Dependent, Immunoprecipitation: Assay Dependent, Western Blot: 1 µg/mL

Species :

Human

Isotype :

IgG

Storage :

-20°C

Description :

p27 Kip1 functions as a negative regulator of G1 mouse expression and has been proposed to function as a possible mediator of TGFb induced G1 arrest. p27 Kip1 is an important regulator of cell cycle progression, a potent inhibitor of cyclin E- and cyclin A-CDK2 complexes. p27 Kip1 forms a complex with cyclin type D-CDK4 complexes and is involved in the assembly, stability, and modulation of CCND1-CDK4 complex activation. p27 Kip1 acts either as an inhibitor or an activator of cyclin type D-CDK4 complexes depending on its phosphorylation state and/or stoichometry. p27 Kip1 has a molecular weight of approximately 27kDa and the degradation of this protein, which is triggered by its CDK dependent phosphorylation and subsequent ubiquitination by SCF complexes, is required for the cellular transition from quiescence to the proliferative state.

Immunogen :

Threonine187-phosphorylated p27 peptide derived from the C-terminus of the human p27 protein

Format :

Liquid

Applications w/Dilutions :

ELISA: Assay Dependent, Immunofluorescence: Assay Dependent, Immunoprecipitation: Assay Dependent, Western Blot: 1 µg/mL

Aliases :

CDKN1B, CDN1B, Cyclin-dependent kinase inhibitor 1B, Cyclin-dependent kinase inhibitor p27, KIP1