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company name :
Invitrogen
other brands :
NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience
product type :
antibody
product name :
Connexin 30 Polyclonal Antibody (Z-PP9)
catalog :
71-2200
quantity :
50 µg
price :
US 430.00
clonality :
polyclonal
host :
domestic rabbit
conjugate :
nonconjugated
clone name :
Z-PP9
reactivity :
African green monkey, human, mouse, rat, cat
application :
western blot, ELISA, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section
more info or order :
citations: 47
Published Application/Species/Sample/DilutionReference
  • immunohistochemistry; African green monkey; 1:500; loading ...; fig 3d
Hosoya M, Fujioka M, Murayama A, Ogawa K, Okano H, Ozawa H. Dynamic Spatiotemporal Expression Changes in Connexins of the Developing Primate's Cochlea. Genes (Basel). 2021;12: pubmed publisher
  • immunocytochemistry; mouse; 1:300; fig 5e
Fukunaga I, Oe Y, Chen C, Danzaki K, Ohta S, Koike A, et al. Activin/Nodal/TGF-β Pathway Inhibitor Accelerates BMP4-Induced Cochlear Gap Junction Formation During in vitro Differentiation of Embryonic Stem Cells. Front Cell Dev Biol. 2021;9:602197 pubmed publisher
  • immunohistochemistry - frozen section; mouse; 1:1000; loading ...; fig 3e
Piantanida A, Acosta L, Brocardo L, Capurro C, Greer C, Rela L. Selective Cre-mediated gene deletion identifies connexin 43 as the main connexin channel supporting olfactory ensheathing cell networks. J Comp Neurol. 2019;527:1278-1289 pubmed publisher
  • immunohistochemistry - frozen section; mouse; 1:400; loading ...; fig 1a
Chen J, Liang C, Zong L, Zhu Y, Zhao H. Knockout of Pannexin-1 Induces Hearing Loss. Int J Mol Sci. 2018;19: pubmed publisher
  • immunohistochemistry; mouse; 1:250; loading ...; fig 1c
Lukashkina V, Levic S, Lukashkin A, Strenzke N, Russell I. A connexin30 mutation rescues hearing and reveals roles for gap junctions in cochlear amplification and micromechanics. Nat Commun. 2017;8:14530 pubmed publisher
  • immunohistochemistry; mouse; loading ...; fig 2l
Fukunaga I, Fujimoto A, Hatakeyama K, Aoki T, Nishikawa A, Noda T, et al. In Vitro Models of GJB2-Related Hearing Loss Recapitulate Ca2+ Transients via a Gap Junction Characteristic of Developing Cochlea. Stem Cell Reports. 2016;7:1023-1036 pubmed publisher
  • immunohistochemistry - frozen section; mouse; 1:75
  • western blot; mouse; 1:500; fig 3
Dianati E, Poiraud J, Weber Ouellette A, Plante I. Connexins, E-cadherin, Claudin-7 and ?-catenin transiently form junctional nexuses during the post-natal mammary gland development. Dev Biol. 2016;416:52-68 pubmed publisher
  • immunohistochemistry - frozen section; mouse; 1:100; fig 7
Stewart M, Plante I, Penuela S, Laird D. Loss of Panx1 Impairs Mammary Gland Development at Lactation: Implications for Breast Tumorigenesis. PLoS ONE. 2016;11:e0154162 pubmed publisher
  • immunohistochemistry - frozen section; mouse; 1:200; fig 6
  • western blot; mouse; 1:250; fig 2
Ramani M, Mylvaganam S, Krawczyk M, Wang L, Zoidl C, Brien J, et al. Differential expression of astrocytic connexins in a mouse model of prenatal alcohol exposure. Neurobiol Dis. 2016;91:83-93 pubmed publisher
  • immunohistochemistry - frozen section; human; 1:100; fig 1
Liu W, Edin F, Blom H, Magnusson P, Schrott Fischer A, Glueckert R, et al. Super-resolution structured illumination fluorescence microscopy of the lateral wall of the cochlea: the Connexin26/30 proteins are separately expressed in man. Cell Tissue Res. 2016;365:13-27 pubmed publisher
  • immunohistochemistry - frozen section; human; fig 3
Kim R, Chang G, Hu R, Phillips A, Douglas R. Connexin gap junction channels and chronic rhinosinusitis. Int Forum Allergy Rhinol. 2016;6:611-7 pubmed publisher
Zhang L, Wang W, Kim S, Wang J, Zhou B, Kong W, et al. Virally Mediated Connexin 26 Expression in Postnatal Scala Media Significantly and Transiently Preserves Hearing in Connexin 30 Null Mice. Front Cell Dev Biol. 2022;10:900416 pubmed publisher
Du Y, Brennan F, Popovich P, Zhou M. Microglia maintain the normal structure and function of the hippocampal astrocyte network. Glia. 2022;70:1359-1379 pubmed publisher
Cheung G, Bataveljic D, Visser J, Kumar N, Moulard J, Dall xe9 rac G, et al. Physiological synaptic activity and recognition memory require astroglial glutamine. Nat Commun. 2022;13:753 pubmed publisher
Lattke M, Goldstone R, Ellis J, Boeing S, Jurado Arjona J, Marichal N, et al. Extensive transcriptional and chromatin changes underlie astrocyte maturation in vivo and in culture. Nat Commun. 2021;12:4335 pubmed publisher
Liu W, Luque M, Li H, Schrott Fischer A, Glueckert R, Tylstedt S, et al. Spike Generators and Cell Signaling in the Human Auditory Nerve: An Ultrastructural, Super-Resolution, and Gene Hybridization Study. Front Cell Neurosci. 2021;15:642211 pubmed publisher
Mozafari S, Deboux C, Laterza C, Ehrlich M, Kuhlmann T, Martino G, et al. Beneficial contribution of induced pluripotent stem cell-progeny to Connexin 47 dynamics during demyelination-remyelination. Glia. 2021;69:1094-1109 pubmed publisher
Gaete P, Lillo M, Lopez W, Liu Y, Jiang W, Luo Y, et al. A novel voltage-clamp/dye uptake assay reveals saturable transport of molecules through CALHM1 and connexin channels. J Gen Physiol. 2020;152: pubmed publisher
Au A, Shao Q, White K, Lucaciu S, Esseltine J, Barr K, et al. Comparative Analysis of Cx31 and Cx43 in Differentiation-Competent Rodent Keratinocytes. Biomolecules. 2020;10: pubmed publisher
Kuang Y, Zorzi V, Buratto D, Ziraldo G, Mazzarda F, Peres C, et al. A potent antagonist antibody targeting connexin hemichannels alleviates Clouston syndrome symptoms in mutant mice. EBioMedicine. 2020;57:102825 pubmed publisher
Abitbol J, Beach R, Barr K, Esseltine J, Allman B, LAIRD D. Cisplatin-induced ototoxicity in organotypic cochlear cultures occurs independent of gap junctional intercellular communication. Cell Death Dis. 2020;11:342 pubmed publisher
Murphy Royal C, Johnston A, Boyce A, Diaz Castro B, Institoris A, Peringod G, et al. Stress gates an astrocytic energy reservoir to impair synaptic plasticity. Nat Commun. 2020;11:2014 pubmed publisher
Beach R, Abitbol J, Allman B, Esseltine J, Shao Q, Laird D. GJB2 Mutations Linked to Hearing Loss Exhibit Differential Trafficking and Functional Defects as Revealed in Cochlear-Relevant Cells. Front Cell Dev Biol. 2020;8:215 pubmed publisher
Fabbiani G, Reali C, Valentin Kahan A, Rehermann M, Fagetti J, Falco M, et al. Connexin Signaling Is Involved in the Reactivation of a Latent Stem Cell Niche after Spinal Cord Injury. J Neurosci. 2020;40:2246-2258 pubmed publisher
Tajima S, Danzaki K, Ikeda K, Kamiya K. Degradation and modification of cochlear gap junction proteins in the early development of age-related hearing loss. Exp Mol Med. 2020;52:166-175 pubmed publisher
Lin X, Li G, Zhang Y, Zhao J, Lu J, Gao Y, et al. Hearing consequences in Gjb2 knock-in mice: implications for human p.V37I mutation. Aging (Albany NY). 2019;11:7416-7441 pubmed publisher
Ghezali G, Vasile F, Curry N, Fantham M, Cheung G, Ezan P, et al. Neuronal Activity Drives Astroglial Connexin 30 in Perisynaptic Processes and Shapes Its Functions. Cereb Cortex. 2019;: pubmed publisher
Huebner A, Maier H, Maul A, Nietzsche S, Herrmann T, Praetorius J, et al. Early Hearing Loss upon Disruption of Slc4a10 in C57BL/6 Mice. J Assoc Res Otolaryngol. 2019;20:233-245 pubmed publisher
Miguel Hidalgo J, Moulana M, Deloach P, Rajkowska G. Chronic Unpredictable Stress Reduces Immunostaining for Connexins 43 and 30 and Myelin Basic Protein in the Rat Prelimbic and Orbitofrontal Cortices. Chronic Stress (Thousand Oaks). 2018;2: pubmed publisher
Ghézali G, Calvo C, Pillet L, Llense F, Ezan P, Pannasch U, et al. Connexin 30 controls astroglial polarization during postnatal brain development. Development. 2018;145: pubmed publisher
Assmus A, Mansley M, Mullins L, Peter A, Mullins J. mCCDcl1 cells show plasticity consistent with the ability to transition between principal and intercalated cells. Am J Physiol Renal Physiol. 2018;314:F820-F831 pubmed publisher
Zorzi V, Paciello F, Ziraldo G, Peres C, Mazzarda F, Nardin C, et al. Mouse Panx1 Is Dispensable for Hearing Acquisition and Auditory Function. Front Mol Neurosci. 2017;10:379 pubmed publisher
Nielsen B, Alstrom J, Nicholson B, Nielsen M, MacAulay N. Permeant-specific gating of connexin 30 hemichannels. J Biol Chem. 2017;292:19999-20009 pubmed publisher
Deshpande T, Li T, Herde M, Becker A, Vatter H, Schwarz M, et al. Subcellular reorganization and altered phosphorylation of the astrocytic gap junction protein connexin43 in human and experimental temporal lobe epilepsy. Glia. 2017;65:1809-1820 pubmed publisher
Lukashkina V, Yamashita T, Zuo J, Lukashkin A, Russell I. Amplification mode differs along the length of the mouse cochlea as revealed by connexin 26 deletion from specific gap junctions. Sci Rep. 2017;7:5185 pubmed publisher
Zong L, Chen J, Zhu Y, Zhao H. Progressive age-dependence and frequency difference in the effect of gap junctions on active cochlear amplification and hearing. Biochem Biophys Res Commun. 2017;489:223-227 pubmed publisher
Liu W, Li H, Edin F, Brannstrom J, Glueckert R, Schrott Fischer A, et al. Molecular composition and distribution of gap junctions in the sensory epithelium of the human cochlea-a super-resolution structured illumination microscopy (SR-SIM) study. Ups J Med Sci. 2017;122:160-170 pubmed publisher
Hattori T, Kaji M, Ishii H, Jureepon R, Takarada Iemata M, Minh Ta H, et al. CD38 positively regulates postnatal development of astrocytes cell-autonomously and oligodendrocytes non-cell-autonomously. Glia. 2017;65:974-989 pubmed publisher
Khan D, Dupper A, Deshpande T, Graan P, Steinhäuser C, Bedner P. Experimental febrile seizures impair interastrocytic gap junction coupling in juvenile mice. J Neurosci Res. 2016;94:804-13 pubmed publisher
Zhu Y, Zong L, Mei L, Zhao H. Connexin26 gap junction mediates miRNA intercellular genetic communication in the cochlea and is required for inner ear development. Sci Rep. 2015;5:15647 pubmed publisher
Alstrøm J, Hansen D, Nielsen M, MacAulay N. Isoform-specific phosphorylation-dependent regulation of connexin hemichannels. J Neurophysiol. 2015;114:3014-22 pubmed publisher
Kelly J, Shao Q, Jagger D, Laird D. Cx30 exhibits unique characteristics including a long half-life when assembled into gap junctions. J Cell Sci. 2015;128:3947-60 pubmed publisher
Bellesi M, de Vivo L, Tononi G, Cirelli C. Effects of sleep and wake on astrocytes: clues from molecular and ultrastructural studies. BMC Biol. 2015;13:66 pubmed publisher
Lee M, Takada T, Takada Y, Kappy M, Beyer L, Swiderski D, et al. Mice with conditional deletion of Cx26 exhibit no vestibular phenotype despite secondary loss of Cx30 in the vestibular end organs. Hear Res. 2015;328:102-12 pubmed publisher
Chen J, Zhu Y, Liang C, Chen J, Zhao H. Pannexin1 channels dominate ATP release in the cochlea ensuring endocochlear potential and auditory receptor potential generation and hearing. Sci Rep. 2015;5:10762 pubmed publisher
Theotokis P, Kleopa K, Touloumi O, Lagoudaki R, Lourbopoulos A, Nousiopoulou E, et al. Connexin43 and connexin47 alterations after neural precursor cells transplantation in experimental autoimmune encephalomyelitis. Glia. 2015;63:1772-83 pubmed publisher
Zhu Y, Liang C, Zong L, Chen J, Jones R, Zhao H. Connexin26 (GJB2) deficiency reduces active cochlear amplification leading to late-onset hearing loss. Neuroscience. 2015;284:719-29 pubmed publisher
product information
Product Type :
Antibody
Product Name :
Connexin 30 Polyclonal Antibody (Z-PP9)
Catalog # :
71-2200
Quantity :
50 µg
Price :
US 430.00
Clonality :
Polyclonal
Purity :
Antigen affinity chromatography
Host :
Rabbit
Reactivity :
Feline, Human, Mouse, Rat
Applications :
ELISA: 0.1-1 µg/mL, Immunocytochemistry: 1:100-1:500, Immunohistochemistry (Frozen): Assay-dependent, Immunohistochemistry (Paraffin): 1:20-1:200, Western Blot: 1-3 µg/mL
Species :
Feline, Human, Mouse, Rat
Clone :
Z-PP9
Isotype :
IgG
Storage :
-20°C
Description :
Gap junctions allow the transport of ions and metabolites between the cytoplasm of adjacent cells. They are formed by two hemichannels, made up of six connexin proteins assembled in groups. Each connexin protein has four transmembrane segments, two extracellular loops, a cytoplasmic loop formed between the two inner transmembrane segments, and the N- and C-terminus both being in the cytoplasm. The specificity of the gap junction is determined by which connexin proteins comprise the hemichannel. In the past, connexin protein names were based on their molecular weight, however the new nomenclature uses sequential numbers based on which form of the gap junction is present. This gene encodes one of the connexin proteins. Mutations in this gene have been found in some forms of deafness and in some families with hidrotic ectodermal dysplasia.
Immunogen :
A synthetic peptide derived from the unique C-terminus of the mouse Connexin 30 protein
Format :
Liquid
Applications w/Dilutions :
ELISA: 0.1-1 µg/mL, Immunocytochemistry: 1:100-1:500, Immunohistochemistry (Frozen): Assay-dependent, Immunohistochemistry (Paraffin): 1:20-1:200, Western Blot: 1-3 µg/mL
Aliases :
AA958971; connexin 30; connexin-30; CX30; Cxn-30; D14Bwg0506e; DFNA3; DFNA3B; DFNB1B; ECTD2; ectodermal dysplasia 2, hidrotic (Clouston syndrome); ED2; EDH; Gap junction beta-6 protein; gap junction membrane channel protein beta 6; gap junction protein beta 6; gap junction protein, beta 6; gap junction protein, beta 6 (connexin 30); gap junction protein, beta 6, 30kDa; Gjb6; HED; HED2
more info or order :
company information
Invitrogen
Thermo Fisher Scientific
81 Wyman Street
Waltham, MA USA 02451
https://www.thermofisher.com
800-678-5599
headquarters: USA