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company name :
Invitrogen
other brands :
NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience
product type :
antibody
product name :
ZO-2 Polyclonal Antibody
catalog :
71-1400
quantity :
100 µg
price :
US 462.00
clonality :
polyclonal
host :
domestic rabbit
conjugate :
nonconjugated
reactivity :
human, mouse, dogs
application :
western blot, ELISA, immunohistochemistry, immunocytochemistry, proximity ligation assay
more info or order :
Invitrogen product webpage
citations: 28
Published Application/Species/Sample/DilutionReference
  • western blot; human; 1:1000; loading ...; fig 3s2a
Fearnley G, Young K, Edgar J, Antrobus R, Hay I, Liang W, et al. The homophilic receptor PTPRK selectively dephosphorylates multiple junctional regulators to promote cell-cell adhesion. elife. 2019;8: pubmed publisher
  • proximity ligation assay; dogs; 1:100
  • immunocytochemistry; dogs; 1:100; loading ...; fig 1a
  • western blot; dogs; 1:1000; loading ...; fig 1a
Raya Sandino A, Castillo Kauil A, Domínguez Calderón A, Alarcón L, Flores Benitez D, Cuellar Perez F, et al. Zonula occludens-2 regulates Rho proteins activity and the development of epithelial cytoarchitecture and barrier function. Biochim Biophys Acta Mol Cell Res. 2017;1864:1714-1733 pubmed publisher
  • immunocytochemistry; dogs; fig 1A
Domínguez Calderón A, Ávila Flores A, Ponce A, López Bayghen E, Calderón Salinas J, Luis Reyes J, et al. ZO-2 silencing induces renal hypertrophy through a cell cycle mechanism and the activation of YAP and the mTOR pathway. Mol Biol Cell. 2016;27:1581-95 pubmed publisher
Lee J, Shaker M, Park S, Sun W. Transcriptional Signature of Valproic Acid-Induced Neural Tube Defects in Human Spinal Cord Organoids. Int J Stem Cells. 2023;16:385-393 pubmed publisher
Higashi T, Stephenson R, Schwayer C, Huljev K, Higashi A, Heisenberg C, et al. Zinc-based Ultrasensitive Microscopic Barrier Assay (ZnUMBA): a live imaging method to detect local barrier breaches. J Cell Sci. 2023;: pubmed publisher
Xu J, Kausalya P, Ong A, Goh C, Mohamed Ali S, Hunziker W. ZO-2/Tjp2 suppresses Yap and Wwtr1/Taz-mediated hepatocyte to cholangiocyte transdifferentiation in the mouse liver. NPJ Regen Med. 2022;7:55 pubmed publisher
Delgado Diaz D, Jesaveluk B, Hayward J, Tyssen D, Alisoltani A, Potgieter M, et al. Lactic acid from vaginal microbiota enhances cervicovaginal epithelial barrier integrity by promoting tight junction protein expression. Microbiome. 2022;10:141 pubmed publisher
Ueda M, Kobayashi H, Seike S, Takahashi E, Okamoto K, Yamanaka H. Aeromonas sobria Serine Protease Degrades Several Protein Components of Tight Junctions and Assists Bacterial Translocation Across the T84 Monolayer. Front Cell Infect Microbiol. 2022;12:824547 pubmed publisher
Bhargavan B, Woollard S, McMillan J, Kanmogne G. CCR5 antagonist reduces HIV-induced amyloidogenesis, tau pathology, neurodegeneration, and blood-brain barrier alterations in HIV-infected hu-PBL-NSG mice. Mol Neurodegener. 2021;16:78 pubmed publisher
Hern xe1 ndez Guzm xe1 n C, Gallego Guti xe9 rrez H, Ch xe1 vez Mungu xed a B, Mart xed n Tapia D, Gonz xe1 lez Mariscal L. Zonula occludens 2 and Cell-Cell Contacts Are Required for Normal Nuclear Shape in Epithelia. Cells. 2021;10: pubmed publisher
Ren Z, Pan L, Huang Y, Chen H, Liu Y, Liu H, et al. Gut microbiota-CRAMP axis shapes intestinal barrier function and immune responses in dietary gluten-induced enteropathy. EMBO Mol Med. 2021;13:e14059 pubmed publisher
Gallego Gutiérrez H, González González L, Ramírez Martínez L, Lopez Bayghen E, Gonzalez Mariscal L. Tight junction protein ZO-2 modulates the nuclear accumulation of transcription factor TEAD. Mol Biol Cell. 2021;32:1347-1358 pubmed publisher
Thomsen M, Humle N, Hede E, Moos T, Burkhart A, Thomsen L. The blood-brain barrier studied in vitro across species. PLoS ONE. 2021;16:e0236770 pubmed publisher
Bai H, Zhou R, Barravecchia M, Norman R, Friedman A, Yu D, et al. The Na+, K+-ATPase β1 subunit regulates epithelial tight junctions via MRCKα. JCI Insight. 2021;6: pubmed publisher
Xu J, Kausalya P, Van Hul N, Caldez M, Xu S, Ong A, et al. Protective Functions of ZO-2/Tjp2 Expressed in Hepatocytes and Cholangiocytes Against Liver Injury and Cholestasis. Gastroenterology. 2021;160:2103-2118 pubmed publisher
Hede E, Christiansen C, Heegaard C, Moos T, Burkhart A. Gene therapy to the blood-brain barrier with resulting protein secretion as a strategy for treatment of Niemann Picks type C2 disease. J Neurochem. 2021;156:290-308 pubmed publisher
Dai W, Nadadur R, Brennan J, Smith H, Shen K, Gadek M, et al. ZO-1 Regulates Intercalated Disc Composition and Atrioventricular Node Conduction. Circ Res. 2020;127:e28-e43 pubmed publisher
Miranda J, Martín Tapia D, Valdespino Vazquez Y, Alarcón L, Espejel Nuñez A, Guzmán Huerta M, et al. Syncytiotrophoblast of Placentae from Women with Zika Virus Infection Has Altered Tight Junction Protein Expression and Increased Paracellular Permeability. Cells. 2019;8: pubmed publisher
Amaya E, Alarcón L, Martín Tapia D, Cuellar Perez F, Cano Cortina M, Ortega Olvera J, et al. Activation of the Ca2+ sensing receptor and the PKC/WNK4 downstream signaling cascade induces incorporation of ZO-2 to tight junctions and its separation from 14-3-3. Mol Biol Cell. 2019;30:2377-2398 pubmed publisher
García Aguirre I, Alamillo Iniesta A, Rodríguez Pérez R, Vélez Aguilera G, Amaro Encarnación E, Jiménez Gutiérrez E, et al. Enhanced nuclear protein export in premature aging and rescue of the progeria phenotype by modulation of CRM1 activity. Aging Cell. 2019;18:e13002 pubmed publisher
Li F, Pascal L, Stolz D, Wang K, Zhou Y, Chen W, et al. E-cadherin is downregulated in benign prostatic hyperplasia and required for tight junction formation and permeability barrier in the prostatic epithelial cell monolayer. Prostate. 2019;79:1226-1237 pubmed publisher
Hsieh J, Rathore A, Soundarajan G, St John A. Japanese encephalitis virus neuropenetrance is driven by mast cell chymase. Nat Commun. 2019;10:706 pubmed publisher
Rodríguez Uribe G, Serafín Higuera N, Damian Morales G, Cortés Malagón E, García Hernández V, Verdejo Torres O, et al. HPV16-E6 Oncoprotein Activates TGF-β and Wnt/β-Catenin Pathways in the Epithelium-Mesenchymal Transition of Cataracts in a Transgenic Mouse Model. Biomed Res Int. 2018;2018:2847873 pubmed publisher
Bilal S, Jaggi S, Janosevic D, Shah N, Teymour S, Voronina A, et al. ZO-1 protein is required for hydrogen peroxide to increase MDCK cell paracellular permeability in an ERK 1/2-dependent manner. Am J Physiol Cell Physiol. 2018;315:C422-C431 pubmed publisher
Cheng Y, Desse S, Martinez A, Worthen R, Jope R, Beurel E. TNFα disrupts blood brain barrier integrity to maintain prolonged depressive-like behavior in mice. Brain Behav Immun. 2018;69:556-567 pubmed publisher
Tello Lafoz M, Martínez Martínez G, Rodríguez Rodríguez C, Albar J, Huse M, Gharbi S, et al. Sorting nexin 27 interactome in T-lymphocytes identifies zona occludens-2 dynamic redistribution at the immune synapse. Traffic. 2017;18:491-504 pubmed publisher
Martínez Revollar G, Garay E, Martín Tapia D, Nava P, Huerta M, Lopez Bayghen E, et al. Heterogeneity between triple negative breast cancer cells due to differential activation of Wnt and PI3K/AKT pathways. Exp Cell Res. 2015;339:67-80 pubmed publisher
Watari A, Hashegawa M, Yagi K, Kondoh M. Homoharringtonine increases intestinal epithelial permeability by modulating specific claudin isoforms in Caco-2 cell monolayers. Eur J Pharm Biopharm. 2015;89:232-8 pubmed publisher
product information
Product Type :
Antibody
Product Name :
ZO-2 Polyclonal Antibody
Catalog # :
71-1400
Quantity :
100 µg
Price :
US 462.00
Clonality :
Polyclonal
Purity :
Antigen affinity chromatography
Host :
Rabbit
Reactivity :
Canine, Human, Mouse
Applications :
ELISA: Assay-dependent, Immunocytochemistry: 1 µg/mL, Immunohistochemistry: Assay-dependent, Western Blot: 1-2 µg/mL
Species :
Canine, Human, Mouse
Isotype :
IgG
Storage :
-20°C
Description :
CD45 (LCA, leukocyte common antigen) is a receptor-type protein tyrosine phosphatase (PTP) ubiquitously expressed in all nucleated hematopoietic cells, comprising approximately 10% of all surface proteins in lymphocytes. CD45 is absent on non-hematopoietic cell lines, normal and malignant, non-hematopoietic tissues. CD45 glycoprotein is crucial in lymphocyte development and antigen signaling, serving as an important regulator of Src-family kinases. CD45 protein exists as multiple isoforms as a result of alternative splicing, differ in their extracellular domains but share identical transmembrane and cytoplasmic domains. CD45RA is an isoform of the CD45 complex and has restricted expression between different subtypes of lymphoid cells. CD45 isoforms differ in their ability to translocate into the glycosphingolipid-enriched membrane domains and their expression depends on cell type and physiological state of the cell. CD45 has been shown to be an essential regulator of T- and B-cell antigen receptor signaling and suppresses JAK kinases to regulate cytokine receptor signaling. CD45 is also important in promoting cell survival by modulating integrin-mediated signal transduction pathway, DNA fragmentation during apoptosis and inhibition or upregulation of various immunological functions.
Immunogen :
Synthetic peptide derived from the central portion of the ZO-2 protein.
Format :
Liquid
Applications w/Dilutions :
ELISA: Assay-dependent, Immunocytochemistry: 1 µg/mL, Immunohistochemistry: Assay-dependent, Western Blot: 1-2 µg/mL
Aliases :
C9DUPq21.11; DFNA51; DUP9q21.11; Friedreich ataxia region gene X104 (tight junction protein ZO-2); PFIC4; tight junction protein; tight junction protein 2; tight junction protein 2 (zona occludens 2); tight junction protein ZO-2; Tight junction protein ZO-2 (Zonula occludens 2 protein) (Zona occludens 2 protein) (Tight junction protein 2); Tjp2; X104; ZO2; ZO-2; zona occludens 2; zona occludens protein 2; Zonula occludens protein 2
more info or order :
Invitrogen product webpage
company information
Invitrogen
Thermo Fisher Scientific
81 Wyman Street
Waltham, MA USA 02451
https://www.thermofisher.com
800-678-5599
headquarters: USA