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company name :
Invitrogen
other brands :
NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience
product type :
antibody
product name :
Phospho-GSK3 alpha/beta (Tyr279, Tyr216) Recombinant Rabbit Monoclonal Antibody (19H1L12)
catalog :
702230
quantity :
100 ug
price :
US 471.00
clonality :
recombinant
host :
domestic rabbit
conjugate :
nonconjugated
antigen modification :
phosphorylated
clone name :
19H1L12
reactivity :
human, mouse
application :
western blot, immunocytochemistry, flow cytometry, immunohistochemistry - paraffin section
more info or order :
Invitrogen product webpage
citations: 1
Published Application/Species/Sample/DilutionReference
  • immunohistochemistry - paraffin section; human; loading ...; fig 7b
Huang W, Bei L, Eklund E. Inhibition of Fas associated phosphatase 1 (Fap1) facilitates apoptosis of colon cancer stem cells and enhances the effects of oxaliplatin. Oncotarget. 2018;9:25891-25902 pubmed publisher
product information
Product Type :
Antibody
Product Name :
Phospho-GSK3 alpha/beta (Tyr279, Tyr216) Recombinant Rabbit Monoclonal Antibody (19H1L12)
Catalog # :
702230
Quantity :
100 ug
Price :
US 471.00
Clonality :
Recombinant Monoclonal
Purity :
protein A
Host :
Rabbit
Reactivity :
Human, Mouse
Applications :
Flow Cytometry: 5 ug x 10^6 cells, Immunocytochemistry: 2 ug/mL, Western Blot: 1-2 ug/mL
Species :
Human, Mouse
Clone :
19H1L12
Isotype :
IgG
Storage :
Store at 4 C short term. For long term storage, store at -20 C, avoiding freeze/thaw cycles.
Description :
Glycogen synthase kinase-3 (GSK3) is a protein kinase that was originally identified as a regulator of glycogen synthase, a key enzyme in glycogen metabolism. Since then, it has been shown to be involved in the regulation of a diverse array of cellular functions, including protein synthesis, cell proliferation, cell differentiation, microtubule assembly/disassembly, and apoptosis. GSK3's substrate specificity is unique in that phosphorylation of the substrate only occurs if a phosphoserine or phosphotyrosine is present - four residues C-terminal to the site of GSK phosphorylation. There exists two isoforms of GSK3, GSK3 alpha and GSK3 beta, and they are strictly regulated via phosphorylation. Phosphorylation of GSK3 beta on Ser9 (Ser21 in GSK3alpha) by protein kinase B (PKB) causes its inactivation and is the primary mechanism responsible for growth factor inhibition of this kinase. Activation of GSK3 beta is dependent upon the phosphorylation of Tyr216 (Tyr279 in GSK3 alpha). Upon activation, it has been shown to phosphorylate a number of different cellular proteins, including p53, c-Myc, c-Jun, heat shock factor-1 (HSF-1), and cyclin D1. GSK3 beta also has been shown to phosphorylate aberrant sites on the microtubule associated protein tau, which is critical for the progression of Alzheimer's disease.
Immunogen :
Peptide corresponding to Human GSK3A, GSK3B (aa 211-220)
Format :
Liquid
Applications w/Dilutions :
Flow Cytometry: 5 ug x 10^6 cells, Immunocytochemistry: 2 ug/mL, Western Blot: 1-2 ug/mL
Aliases :
2700086H06Rik; 7330414F15Rik; 8430431H08Rik; C86142; FA; factor A; glycogen synthase kinase 3 alpha; glycogen synthase kinase 3 beta; glycogen synthase kinase-3 alpha; Glycogen synthase kinase-3 beta; GSK3; GSK-3; GSK-3 alpha; GSK-3 beta; Gsk3a; gsk-3-alpha; Gsk3b; GSK-3beta; GSK3beta isoform; serine/threonine-protein kinase GSK3A; Serine/threonine-protein kinase GSK3B
more info or order :
Invitrogen product webpage
company information
Invitrogen
Thermo Fisher Scientific
81 Wyman Street
Waltham, MA USA 02451
https://www.thermofisher.com
800-678-5599
headquarters: USA