SMAD4 Recombinant Rabbit Monoclonal Antibody (10H10L15) | Invitrogen 701682 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

SMAD4 Recombinant Rabbit Monoclonal Antibody (10H10L15)

catalog :

701682

quantity :

100 ug

price :

US 520.00

clonality :

recombinant

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

10H10L15

reactivity :

human

application :

western blot, immunocytochemistry, immunoprecipitation, RNA immunoprecipitation

product information

Product Type :

Antibody

Product Name :

SMAD4 Recombinant Rabbit Monoclonal Antibody (10H10L15)

Catalog # :

701682

Quantity :

100 ug

Price :

US 520.00

Clonality :

Recombinant Monoclonal

Purity :

protein A

Host :

Rabbit

Reactivity :

Human

Applications :

Immunocytochemistry: 2 ug/mL, Immunoprecipitation: Assay-dependent, RNA Immunoprecipitation: Assay-dependent, Western Blot: 0.5-2 ug/mL

Species :

Human

Clone :

10H10L15

Isotype :

IgG

Storage :

Store at 4 C short term. For long term storage, store at -20 C, avoiding freeze/thaw cycles.

Description :

Smad4 is encoded by the Smad4 gene that is located on chromosome 18 in humans. Smad4 is also known as Smad family member 4, Sma- and Mad-relatedprotein 4 or Mothers against decapentaplegic (MAD) homolog 4. Smad4 contains N-terminal MH1 (MAD homology 1) and C-terminal MH2 (MAD homology 2) globular domains that are involved in DNA binding and protein interactions respectively. Binding of the TGF-beta superfamily of ligands that includes Transforming Growth Factor -beta (TGF-beta) and bone morphogenetic protein (BMP) to its cognate receptor allows phosphorylation of Smad 1, 2, 3, 5 and 8 (R-Smad, Receptor Smad). This signals for heterotrimerization with Smad4 (co-Smad, co-mediator Smad) and translocation of the complex to the nucleus. Inhibitory or antagonistic Smad (I-Smad) that includes Smad 6 and 7, interact with activated R-Smads and attenuate the signaling pathway. Smad4 acts as a tumor suppressor protein by transcriptionally regulating its target genes such as Cyclin D1 (downregulation) and collagen (upregulation) that inhibit cell proliferation. Dephosphorylation regulates nuclear export and nucleocytoplasmic dynamics of Smads.

Immunogen :

full-length protein corresponding to human SMAD4 (aa 1-552)

Format :

Liquid

Applications w/Dilutions :

Immunocytochemistry: 2 ug/mL, Immunoprecipitation: Assay-dependent, RNA Immunoprecipitation: Assay-dependent, Western Blot: 0.5-2 ug/mL

Aliases :

AW743858; D18Wsu70e; Deleted in Pancreatic Carcinoma 4 (DPC4); deleted in pancreatic carcinoma locus 4; deletion target in pancreatic carcinoma 4; Deletion target in pancreatic carcinoma 4 homolog; DPC4; hSMAD4; JIP; MAD (mothers against decapentaplegic Drosophila) homolog 4; MAD homolog 4; MAD homolog 4 (MADH4); Madh4; mothers against decapentaplegic homolog 4; Mothers against decapentaplegic homolog 4 (MADH4); mothers against decapentaplegic, Drosophila, homolog of, 4; mothers against DPP homolog 4; MYHRS; SMAD; SMAD 4; SMAD family member 4; SMAD, mothers against DPP homolog 4; SMAD4