Cyclin D1 Recombinant Rabbit Monoclonal Antibody (17H3L3) | Invitrogen 701421 product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

Cyclin D1 Recombinant Rabbit Monoclonal Antibody (17H3L3)

catalog :

701421

quantity :

100 µg

price :

US 375.00

clonality :

recombinant

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

17H3L3

reactivity :

human, mouse, rat

application :

western blot, immunocytochemistry

more info or order :

Invitrogen product webpage

citations: 2

Published Application/Species/Sample/Dilution	Reference
western blot; human; 1:500; fig 1c	Mende N, Kuchen E, Lesche M, Grinenko T, Kokkaliaris K, Hanenberg H, et al. CCND1-CDK4-mediated cell cycle progression provides a competitive advantage for human hematopoietic stem cells in vivo. J Exp Med. 2015;212:1171-83 pubmed publisher
	Rubinstein M, Baik J, Lagana S, Han R, Raab W, Sahoo D, et al. Fusobacterium nucleatum promotes colorectal cancer by inducing Wnt/β-catenin modulator Annexin A1. EMBO Rep. 2019;20: pubmed publisher

product information

Product Type :

Antibody

Product Name :

Cyclin D1 Recombinant Rabbit Monoclonal Antibody (17H3L3)

Catalog # :

701421

Quantity :

100 µg

Price :

US 375.00

Clonality :

Recombinant Monoclonal

Purity :

protein A

Host :

Rabbit

Reactivity :

Human, Mouse, Rat

Applications :

Immunocytochemistry: 1:100-1:1,000, Western Blot: 1:500-1:5,000

Species :

Human, Mouse, Rat

Clone :

17H3L3

Isotype :

IgG

Storage :

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Description :

Cyclin D1 (PRAD-1, bcl-1) is one of the key cell cycle regulators, and functions in association with cdk4 and/or cdk6 by phosphorylating the Rb protein. Cyclin D1 is a putative proto-oncogene overexpressed in a wide variety of human neoplasms including mantle cell lymphomas (MCL). In addition, cyclin D1 positively regulates protein phosphorylation, mammary gland epithelial cell proliferation, and fat cell differentiation. In humans, the CCND1 gene encoding cyclin D1 is present on chromosome 11. Cyclin D1 is a cytoplasmic and nuclear protein that is synthesized during G1 phase and assembles with either cyclin-dependent kinase 4 (CDK4) or CDK6 in response to growth factor stimulation. D-type cyclin-CDK complexes act to inactivate the growth-suppressive function of the Rb protein through its phosphorylation, and titrate CDK inhibitors such as p21Cip1 and p27Kip1. Without growth factor-mediated stimulation, Cyclin D1 is unstable, and undergoes ubiquitin-mediated degradation, which is triggered by its phosphorylation. Cyclin D1 destabilization participates in G1/S phase arrest. The Cyclin D1 protein belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance throughout the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. Cyclin D1 forms a complex with and functions as a regulatory subunit of CDK4 or CDK6, whose activity is required for cell cycle G1/S transition. The Cyclin D1 protein has been shown to interact with tumor suppressor protein Rb and the expression of this gene is regulated positively by Rb. Mutations, amplification and overexpression of this gene, which alters cell cycle progression, are observed frequently in a variety of tumors and may contribute to tumorigenesis. Cyclin D1 has been successfully employed and is a promising tool for further studies in both cell cycle biology and cancer associated abnormalities.

Immunogen :

Peptide corresponding to amino acids 167–180 of human Cyclin D1

Format :

Liquid

Applications w/Dilutions :

Immunocytochemistry: 1:100-1:1,000, Western Blot: 1:500-1:5,000

Aliases :

AI327039; B-cell CLL/lymphoma 1; B-cell lymphoma 1 protein; BCL1; BCL-1; BCL-1 oncogene; CCND1; CCND1 protein; cD1; cDNA; CycD1; cyclin D1; Cyl-1; D11S287E; FLJ93625; G1/S-specific cyclin-D1; mRNA; PRAD1; PRAD-1; PRAD1 oncogene; U21B31

more info or order :

Invitrogen product webpage