Occludin Recombinant Rabbit Monoclonal Antibody (6H10L9) | Invitrogen 701161 product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

Occludin Recombinant Rabbit Monoclonal Antibody (6H10L9)

catalog :

701161

quantity :

100 µg

price :

US 375.00

clonality :

recombinant

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

6H10L9

reactivity :

human, pigs

application :

western blot, immunocytochemistry

more info or order :

Invitrogen product webpage

citations: 7

Published Application/Species/Sample/Dilution	Reference
western blot; pigs ; fig 6A	Springler A, Hessenberger S, Schatzmayr G, Mayer E. Early Activation of MAPK p44/42 Is Partially Involved in DON-Induced Disruption of the Intestinal Barrier Function and Tight Junction Network. Toxins (Basel). 2016;8: pubmed publisher
western blot; human; 1:3000; loading ...; fig 8	Grozdanović M, ÄŒaviÄ‡ M, NeÅ¡iÄ‡ A, AndjelkoviÄ‡ U, Akbari P, Smit J, et al. Kiwifruit cysteine protease actinidin compromises the intestinal barrier by disrupting tight junctions. Biochim Biophys Acta. 2016;1860:516-26 pubmed publisher
western blot; human	Li F, Duggal R, Oliva O, Karki S, Surolia R, Wang Z, et al. Heme oxygenase-1 protects corexit 9500A-induced respiratory epithelial injury across species. PLoS ONE. 2015;10:e0122275 pubmed publisher
	Gao X, Acharya B, Engl W, de Mets R, Thiery J, Yap A, et al. Probing compression versus stretch activated recruitment of cortical actin and apical junction proteins using mechanical stimulations of suspended doublets. APL Bioeng. 2018;2:026111 pubmed publisher
	Zinner B, Gyöngyösi B, Babarczi E, Kiss A, Sobel G. Claudin 1 expression characterizes human uterine cervical reserve cells. J Histochem Cytochem. 2013;61:880-8 pubmed publisher
	Eker B, Meissner R, Bertsch A, Mehta K, Renaud P. Label-free recognition of drug resistance via impedimetric screening of breast cancer cells. PLoS ONE. 2013;8:e57423 pubmed publisher
	Meissner R, Eker B, Kasi H, Bertsch A, Renaud P. Distinguishing drug-induced minor morphological changes from major cellular damage via label-free impedimetric toxicity screening. Lab Chip. 2011;11:2352-61 pubmed publisher

product information

Product Type :

Antibody

Product Name :

Occludin Recombinant Rabbit Monoclonal Antibody (6H10L9)

Catalog # :

701161

Quantity :

100 µg

Price :

US 375.00

Clonality :

Recombinant Monoclonal

Purity :

protein A

Host :

Rabbit

Reactivity :

Human

Applications :

Immunocytochemistry: 1:100-1:1,000, Western Blot: 1:500-1:5,000

Species :

Human

Clone :

6H10L9

Isotype :

IgG

Storage :

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Description :

Occludin is a 65 kDa protein that can exist in a variety of phosphorylated forms, ranging up to approximately 82 kDa. Occludin is thought to be involved in regulating both the localization and the function of occludin. Polyunsaturated fatty acids are known to up-regulate occludin expression, increasing the transendothelial cell resistance and reducing the cellular permeability to large molecules. The level of occludin varies greatly depending on tissue; in brain tissue, occludin is highly and continuously expressed at cell-cell contact sites, whereas non-neural tissues show lower expression and discontinuous distribution. Overall structural features of the occludin protein are highly conserved in all the species examined. Under-expression of tight junction proteins, including occludin, is a key molecular abnormality responsible for the increased permeability of tumor endothelial tight junctions, which contributes to brain tumor edemas.

Immunogen :

Peptide corresponding to amino acids 387–403 of human occludin

Format :

Liquid

Applications w/Dilutions :

Immunocytochemistry: 1:100-1:1,000, Western Blot: 1:500-1:5,000

Aliases :

AI503564; BLCPMG; Occludin; occludin 1B; Ocl; Ocln; phosphatase 1, regulatory subunit 115; PPP1R115; tight junction protein occludin; tight junction structural protein

more info or order :

Invitrogen product webpage