MDM2 Recombinant Rabbit Monoclonal Antibody (4H26L4) | Invitrogen 700555 product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

MDM2 Recombinant Rabbit Monoclonal Antibody (4H26L4)

catalog :

700555

quantity :

100 ug

price :

US 471.00

clonality :

recombinant

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

4H26L4

reactivity :

human, mouse

application :

western blot, immunocytochemistry

more info or order :

Invitrogen product webpage

citations: 7

Published Application/Species/Sample/Dilution	Reference
western blot; human; loading ...; fig 1	Reuther C, Heinzle V, Nölting S, Herterich S, Hahner S, Halilovic E, et al. The HDM2 (MDM2) Inhibitor NVP-CGM097 Inhibits Tumor Cell Proliferation and Shows Additive Effects with 5-Fluorouracil on the p53-p21-Rb-E2F1 Cascade in the p53wild type Neuroendocrine Tumor Cell Line GOT1. Neuroendocrinology. 2018;106:1-19 pubmed publisher
western blot; human; loading ...; fig 3a, b	Tian X, Dai S, Sun J, Jiang S, Sui C, Meng F, et al. Inhibition of MDM2 Re-Sensitizes Rapamycin Resistant Renal Cancer Cells via the Activation of p53. Cell Physiol Biochem. 2016;39:2088-2098 pubmed
western blot; human; 1:300; loading ...; fig 2b	Qi D, Cobrinik D. MDM2 but not MDM4 promotes retinoblastoma cell proliferation through p53-independent regulation of MYCN translation. Oncogene. 2017;36:1760-1769 pubmed publisher
	Tran H, Singh H, Guo W, Cambier L, Riggan L, Shackleford G, et al. Reciprocal Induction of MDM2 and MYCN in Neural and Neuroendocrine Cancers. Front Oncol. 2020;10:563156 pubmed publisher
	Hubackova S, Davidova E, Boukalova S, Kovarova J, Bajzikova M, Coelho A, et al. Replication and ribosomal stress induced by targeting pyrimidine synthesis and cellular checkpoints suppress p53-deficient tumors. Cell Death Dis. 2020;11:110 pubmed publisher
	Neochoritis C, Atmaj J, Twarda Clapa A, Surmiak E, Skalniak L, Köhler L, et al. Hitting on the move: Targeting intrinsically disordered protein states of the MDM2-p53 interaction. Eur J Med Chem. 2019;182:111588 pubmed publisher
	Skalniak L, Kocik J, Polak J, Skalniak A, Rak M, Wolnicka Glubisz A, et al. Prolonged Idasanutlin (RG7388) Treatment Leads to the Generation of p53-Mutated Cells. Cancers (Basel). 2018;10: pubmed publisher

product information

Product Type :

Antibody

Product Name :

MDM2 Recombinant Rabbit Monoclonal Antibody (4H26L4)

Catalog # :

700555

Quantity :

100 ug

Price :

US 471.00

Clonality :

Recombinant Monoclonal

Purity :

protein A

Host :

Rabbit

Reactivity :

Human, Mouse

Applications :

Immunocytochemistry: 1-3 ug/mL, Western Blot: 0.1-1 ug/mL

Species :

Human, Mouse

Clone :

4H26L4

Isotype :

IgG

Storage :

Store at 4 C short term. For long term storage, store at -20 C, avoiding freeze/thaw cycles.

Description :

MDM2 is a ubiquitin ligase for p53 and plays a central role in regulation of the stability of p53. MDM2 is located on chromosome 12 on the q arm. Akt-mediated phosphorylation of MDM2 at Ser166 and Ser186 increases its interaction with p300, allowing MDM2-mediated ubiquitination and degradation of p53. Phosphorylation of MDM2 also blocks its binding to p19ARF, increasing the degradation of p53. MDM2 has also been shown to negatively regulate p53 function. MDM2 binds and inhibits transactivation role played by p53 and overexpression of MDM2 can result in the inactivation of p53 and decrease its tumor suppressor function. Another process by which MDM2 can inactivate p53 is by degrading p53 as the protein also possesses E3 ubiquitin ligase activity. Further, MDM2 plays important roles in apoptosis and cell cycle. MDM2 is over expressed in a wide range of human malignancies including soft tissue carcinomas and breast cancer. In addition to p53, MDM2 is involved in processes of cell cycle, apoptosis, and tumorigenesis through interactions with proteins that include retinoblastoma 1 and ribosomal protein L5. More than 40 different alternatively spliced transcript variants of MDM2 have been isolated from both tumor and normal tissues.

Immunogen :

Recombinant protein corresponding to amino acids 215-422 of human MDM2.

Format :

Liquid

Applications w/Dilutions :

Immunocytochemistry: 1-3 ug/mL, Western Blot: 0.1-1 ug/mL

Aliases :

1700007J15Rik; AA415488; ACTFS; double minute 2; double minute 2 protein; double minute 2, human homolog of; p53-binding protein; E3 ubiquitin-protein ligase Mdm2; Hdm2; HDMX; HGNC:6973; human homolog of; MDM2; Mdm-2; MDM2 oncogene, E3 ubiquitin protein ligase; Mdm2 p53 binding protein homolog; MDM2 proto-oncogene; MDM2 proto-oncogene, E3 ubiquitin protein ligase; MDM2 variant FB28; Mdm2, p53 E3 ubiquitin protein ligase homolog; Mdm2, transformed 3T3 cell double minute 2, p53 binding protein; MGC5370; MGC71221; oncoprotein Mdm2; OTTHUMP00000183490; OTTHUMP00000183492; OTTHUMP00000183494; OTTHUMP00000183495; OTTHUMP00000183496; OTTHUMP00000183497; p53 E3 ubiquitin protein ligase; p53-binding protein Mdm2; RING-type E3 ubiquitin transferase Mdm2; transformed mouse 3T3 cell double minute 2

more info or order :

Invitrogen product webpage