HIF1A Recombinant Rabbit Monoclonal Antibody (16H4L13) | Invitrogen 700505 product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

HIF1A Recombinant Rabbit Monoclonal Antibody (16H4L13)

catalog :

700505

quantity :

100 ug

price :

US 483.00

clonality :

recombinant

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

16H4L13

reactivity :

human

application :

western blot, ELISA, immunocytochemistry

more info or order :

Invitrogen product webpage

citations: 7

Published Application/Species/Sample/Dilution	Reference
western blot; human; 1:250; loading ...; fig s1a	Lima L, Gaiteiro C, Peixoto A, Soares J, Neves M, Santos L, et al. Reference Genes for Addressing Gene Expression of Bladder Cancer Cell Models under Hypoxia: A Step Towards Transcriptomic Studies. PLoS ONE. 2016;11:e0166120 pubmed publisher
western blot; human; 1:500; fig 6	Wood L, Cox N, Phelps C, Lai S, Poddar A, Talbot C, et al. Thyroid Transcription Factor 1 Reprograms Angiogenic Activities of Secretome. Sci Rep. 2016;6:19857 pubmed publisher
	H xe4 kli M, Kreutzer J, M xe4 ki A, V xe4 lim xe4 ki H, Cherian R, Kallio P, et al. Electrophysiological Changes of Human-Induced Pluripotent Stem Cell-Derived Cardiomyocytes during Acute Hypoxia and Reoxygenation. Stem Cells Int. 2022;2022:9438281 pubmed publisher
	Gaballah M, Penttinen K, Kreutzer J, M xe4 ki A, Kallio P, Aalto Set xe4 l xe4 K. Cardiac Ischemia On-a-Chip: Antiarrhythmic Effect of Levosimendan on Ischemic Human-Induced Pluripotent Stem Cell-Derived Cardiomyocytes. Cells. 2022;11: pubmed publisher
	H xe4 kli M, Kreutzer J, M xe4 ki A, V xe4 lim xe4 ki H, Lappi H, Huhtala H, et al. Human induced pluripotent stem cell-based platform for modeling cardiac ischemia. Sci Rep. 2021;11:4153 pubmed publisher
	Rana N, Singh P, Koch B. CoCl2 simulated hypoxia induce cell proliferation and alter the expression pattern of hypoxia associated genes involved in angiogenesis and apoptosis. Biol Res. 2019;52:12 pubmed publisher
	Lazaridis I, Nadel D, Rollefson G, Merrett D, Rohland N, Mallick S, et al. Genomic insights into the origin of farming in the ancient Near East. Nature. 2016;536:419-24 pubmed

product information

Product Type :

Antibody

Product Name :

HIF1A Recombinant Rabbit Monoclonal Antibody (16H4L13)

Catalog # :

700505

Quantity :

100 ug

Price :

US 483.00

Clonality :

Recombinant Monoclonal

Purity :

protein A

Host :

Rabbit

Reactivity :

Human

Applications :

ChIP Assay: 1 uL, ELISA: Assay-dependent, Immunocytochemistry: 1:100-1:500, Western Blot: 1:500-1:5,000

Species :

Human

Clone :

16H4L13

Isotype :

IgG

Storage :

Store at 4 C short term. For long term storage, store at -20 C, avoiding freeze/thaw cycles.

Description :

HIF1-alpha (HIF1A) is a subunit of HIF1, which is a transcription factor found in mammalian cells cultured under reduced oxygen tension. HIF-1 is a heterodimer consisting of an alpha and beta subunit, both belonging to the basic-helix-loop-helix Per-aryl hydrocarbon receptor nuclear translocator-Sim (PAS) family of transcription factors. HIF1 functions as a transcriptional regulator of the adaptive response to hypoxia. Under hypoxic conditions, HIF-1 activates the transcription of over 40 genes, including erythropoietin, glucose transporters, glycolytic enzymes, vascular endothelial growth factor, HILPDA, and other genes whose protein products increase oxygen delivery or facilitate metabolic adaptation to hypoxia. HIF1-alpha regulates hypoxia-mediated apoptosis, cell proliferation and tumor angiogenesis. Hypoxia which induces p53 protein accumulation, directly interacts with HIF1-alpha and reduces hypoxia-induced expression of HIF1-alpha by promoting MDM2-mediated ubiquitination and proteasomal degradation under hypoxic conditions. Recent studies suggest that induction of NOX4 by HIF1-alpha contributes to maintain ROS levels after hypoxia and hypoxia-induced proliferation. In humans, it is located on the q arm of chromosome 14. The C-terminal of HIF1A binds to p300. p300/CBP-HIF complexes participate in the induction of hypoxia-responsive genes, including VEGF. Hypoxia contributes significantly to the pathophysiology of major categories of human disease, including myocardial and cerebral ischemia, cancer, pulmonary hypertension, congenital heart disease and chronic obstructive pulmonary disease.

Immunogen :

proprietary

Format :

Liquid

Applications w/Dilutions :

ChIP Assay: 1 uL, ELISA: Assay-dependent, Immunocytochemistry: 1:100-1:500, Western Blot: 1:500-1:5,000

Aliases :

AA959795; ARNT interacting protein; ARNT2; ARNT-interacting protein; Basic-helix-loop-helix-PAS protein MOP1; bHLHe78; Class E basic helix-loop-helix protein 78; HIF; hif 1; hif 1a; HIF1; HIF1 alpha; HIF-1 alpha; HIF1a; hif-1a; hif1a.L; HIF1alpha; HIF-1alpha; HIF-1-alpha; HIF1-alpha; HIF1-alpha protein; hypoxia inducible factor 1 alpha; hypoxia inducible factor 1 alpha subunit; hypoxia inducible factor 1 alpha subunit L homeolog; hypoxia inducible factor 1 subunit alpha; hypoxia inducible factor 1 subunit alpha L homeolog; hypoxia inducible factor 1, alpha subunit; hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor); hypoxia inducible factor-1 alpha; hypoxia-inducible factor 1 alpha; hypoxia-inducible factor 1 alpha isoform I.3; hypoxia-inducible factor 1 alpha subunit; hypoxia-inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor); hypoxia-inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor) L homeolog; hypoxia-inducible factor 1-alpha; hypoxia-inducible factor-1 alpha; hypoxia-inducible factor1alpha; member of PAS protein 1; member of PAS superfamily 1; MOP1; PAS domain-containing protein 8; PASD8; XELAEV_18039792mg; XELAEV_18041137mg; xhif1a

more info or order :

Invitrogen product webpage