Phospho-AKT1 (Ser473) Recombinant Rabbit Monoclonal Antibody (98H9L8)

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

Phospho-AKT1 (Ser473) Recombinant Rabbit Monoclonal Antibody (98H9L8)

catalog :

700392

quantity :

100 ug

price :

US 450.00

clonality :

recombinant

host :

domestic rabbit

conjugate :

nonconjugated

antigen modification :

phosphorylated

clone name :

98H9L8

reactivity :

human, mouse

application :

western blot, ELISA, immunohistochemistry, immunocytochemistry, flow cytometry, immunohistochemistry - paraffin section

more info or order :

Invitrogen product webpage

citations: 19

Published Application/Species/Sample/Dilution	Reference
flow cytometry; human; fig 4	Rebolleda N, Losada Fernandez I, Perez Chacon G, Castejon R, Rosado S, Morado M, et al. Synergistic Activity of Deguelin and Fludarabine in Cells from Chronic Lymphocytic Leukemia Patients and in the New Zealand Black Murine Model. PLoS ONE. 2016;11:e0154159 pubmed publisher
immunohistochemistry - paraffin section; human; 1:500; fig 2	Zaldumbide L, Erramuzpe A, Guarch R, Pulido R, CortÃ©s J, LÃ³pez J. Snail heterogeneity in clear cell renal cell carcinoma. BMC Cancer. 2016;16:194 pubmed publisher
western blot; human; fig 4b	Seashore Ludlow B, Rees M, Cheah J, Cokol M, Price E, Coletti M, et al. Harnessing Connectivity in a Large-Scale Small-Molecule Sensitivity Dataset. Cancer Discov. 2015;5:1210-23 pubmed publisher
western blot; human	Coon B, Baeyens N, Han J, Budatha M, Ross T, Fang J, et al. Intramembrane binding of VE-cadherin to VEGFR2 and VEGFR3 assembles the endothelial mechanosensory complex. J Cell Biol. 2015;208:975-86 pubmed publisher
immunohistochemistry - paraffin section; human; 1:100	Torres A, Nogueira C, Magalhães J, Costa I, Aragao A, Gomes Neto A, et al. Expression of EGFR and molecules downstream to PI3K/Akt, Raf-1-MEK-1-MAP (Erk1/2), and JAK (STAT3) pathways in invasive lung adenocarcinomas resected at a single institution. Anal Cell Pathol (Amst). 2014;2014:352925 pubmed publisher
	Lopardo V, Montella F, Esposito R, Zannella C, Aliberti S, Capunzo M, et al. SARS-CoV-2 Lysate Stimulation Impairs the Release of Platelet-like Particles and Megakaryopoiesis in the MEG-01 Cell Line. Int J Mol Sci. 2023;24: pubmed publisher
	Trefny M, Kirchhammer N, Auf der Maur P, Natoli M, Schmid D, Germann M, et al. Deletion of SNX9 alleviates CD8 T cell exhaustion for effective cellular cancer immunotherapy. Nat Commun. 2023;14:86 pubmed publisher
	Kim H, Kim T, Kim Y, Seong C, Cho J, Kim W, et al. Antioxidant and Antiproliferative Activity of Finasteride against Glioblastoma Cells. Pharmaceutics. 2021;13: pubmed publisher
	Nakao M, Shimizu I, Katsuumi G, Yoshida Y, Suda M, Hayashi Y, et al. Empagliflozin maintains capillarization and improves cardiac function in a murine model of left ventricular pressure overload. Sci Rep. 2021;11:18384 pubmed publisher
	Villegas Mendez A, Khandelwal G, McGowan L, Dookie R, Haley M, George C, et al. Exhausted CD4+ T Cells during Malaria Exhibit Reduced mTORc1 Activity Correlated with Loss of T-bet Expression. J Immunol. 2020;205:1608-1619 pubmed publisher
	Chang R, Thomas K, Bedi Y, Golding M. Programmed increases in LXRα induced by paternal alcohol use enhance offspring metabolic adaptation to high-fat diet induced obesity. Mol Metab. 2019;30:161-172 pubmed publisher
	Conduit S, Davies E, Ooms L, Gurung R, McGrath M, Hakim S, et al. AKT signaling promotes DNA damage accumulation and proliferation in polycystic kidney disease. Hum Mol Genet. 2020;29:31-48 pubmed publisher
	Chang R, Wang H, Bedi Y, Golding M. Preconception paternal alcohol exposure exerts sex-specific effects on offspring growth and long-term metabolic programming. Epigenetics Chromatin. 2019;12:9 pubmed publisher
	Liu S, Chen L, Xu Y. Significance of PYK2 level as a prognosis predictor in patients with colon adenocarcinoma after surgical resection. Onco Targets Ther. 2018;11:7625-7634 pubmed publisher
	Talebi A, Dehairs J, Rambow F, Rogiers A, Nittner D, Derua R, et al. Sustained SREBP-1-dependent lipogenesis as a key mediator of resistance to BRAF-targeted therapy. Nat Commun. 2018;9:2500 pubmed publisher
	Ibrahim M, Scozzi D, Toth K, Ponti D, Kreisel D, Menna C, et al. Naive CD4+ T Cells Carrying a TLR2 Agonist Overcome TGF-β-Mediated Tumor Immune Evasion. J Immunol. 2018;200:847-856 pubmed publisher
	Cheng C, Jia K, Chen T, Chang S, Lin M, Yin H. Experimentally validated novel inhibitors of Helicobacter pylori phosphopantetheine adenylyltransferase discovered by virtual high-throughput screening. PLoS ONE. 2013;8:e74271 pubmed publisher
	Steketee M, Moysidis S, Jin X, Weinstein J, Pita Thomas W, Raju H, et al. Nanoparticle-mediated signaling endosome localization regulates growth cone motility and neurite growth. Proc Natl Acad Sci U S A. 2011;108:19042-7 pubmed publisher
	Castillo Rojas G, Cerbón M, Lopez Vidal Y. Presence of Helicobacter pylori in a Mexican Pre-Columbian Mummy. BMC Microbiol. 2008;8:119 pubmed publisher

product information

Product Type :

Antibody

Product Name :

Phospho-AKT1 (Ser473) Recombinant Rabbit Monoclonal Antibody (98H9L8)

Catalog # :

700392

Quantity :

100 ug

Price :

US 450.00

Clonality :

Recombinant Monoclonal

Purity :

protein A

Host :

Rabbit

Reactivity :

Human, Mouse

Applications :

ELISA: 1-5 ug/mL, Flow Cytometry: 1-3 ug/1x10^6 cells, Immunocytochemistry: 1-2 ug, Immunohistochemistry (Paraffin): 0.5-2 ug/mL, Western Blot: 1-3 ug/mL

Species :

Human, Mouse

Clone :

98H9L8

Isotype :

IgG

Storage :

Store at 4 C short term. For long term storage, store at -20 C, avoiding freeze/thaw cycles.

Description :

AKT1 (PKB alpha) is a serine/threonine kinase that regulates cell survival. The activated enzyme inhibits apoptosis and stimulates cell cycle progression by phosphorylating numerous targets in various cell types, including cancer cells. This protein kinase is activated by insulin, PI3K, IGF1 and various other growth and survival factors. Akt promotes cell survival by inhibiting apoptosis through phosphorylation and inactivation of several targets, including forkhead transcription factors, and caspase-9. The AKT pathway is a major target for cancer drug discovery.

Immunogen :

A peptide corresponding to amino acids 468-477 of P31749.

Format :

Liquid

Applications w/Dilutions :

ELISA: 1-5 ug/mL, Flow Cytometry: 1-3 ug/1x10^6 cells, Immunocytochemistry: 1-2 ug, Immunohistochemistry (Paraffin): 0.5-2 ug/mL, Western Blot: 1-3 ug/mL

Aliases :

Akt; Akt kinase; AKT serine/threonine kinase 1; Akt/PKB; akt1; AKT1 kinase; AKT1m; Akt1m protein; Akt1-PA; Akt1-PB; Akt1-PC; Akt1-PD; Akt1-PE; CAKT; C-AKT; CG4006; CG4006-PA; CG4006-PB; CG4006-PC; CG4006-PD; CG4006-PE; CWS6; dAkt; D-Akt; dAkt kinase; dAKT/dPKB; dAkt/PKB; DAKT1; DAKT1/PKB; Dmel CG4006; Dmel_CG4006; dPKB; DRAC-PK; DRAC-PK66; DRAC-PK85; l(3)04226; l(3)89Bq; murine thymoma viral (v-akt) oncogene homolog 1; pAkt; P-AKT; PKB; PKB alpha; PKB beta; PKB gamma; PKB/AKT; PKB/dAKT; PKBalpha; PKB-ALPHA; PKBG; PRKBA; protein kinase B; Protein kinase B alpha; protein kinase B-alpha; proto-oncogene c-Akt; RAC; rac protein kinase alpha; RAC protein kinase alpha RAC-PK alpha; RAC serine/threonine protein kinase; RAC serine/threonine-protein kinase; RAC-ALPHA; RAC-alpha serine/threonine-protein kinase; RacPK; RAC-PK-alpha; RAC-PK-beta; RAC-PK-gamma; related to A and C kinases; related to PKA to PKC protein kinases; related to the A and C kinases; SD10374p; serine/threonine protein kinase; serine-threonine protein kinase; Thymoma viral proto-oncogene; thymoma viral proto-oncogene 1; v-akt murine thymoma viral oncogene homolog 1; v-akt murine thymoma viral oncogene-like protein 1

more info or order :

Invitrogen product webpage