EGFR Recombinant Rabbit Monoclonal Antibody (30H45L48) | Invitrogen 700308 product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

EGFR Recombinant Rabbit Monoclonal Antibody (30H45L48)

catalog :

700308

quantity :

100µg

price :

US 345

clonality :

recombinant

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

30H45L48

reactivity :

human

application :

western blot, immunocytochemistry

more info or order :

Invitrogen product webpage

citations: 4

Published Application/Species/Sample/Dilution	Reference
western blot; human; loading ...; fig 5a	Toyama K, Mizuguchi T, Nomura W, Tamamura H. Functional evaluation of fluorescein-labeled derivatives of a peptide inhibitor of the EGF receptor dimerization. Bioorg Med Chem. 2016;24:3406-12 pubmed publisher
immunocytochemistry; human; loading ...; fig 5a	Ren D, Xia Y, Wang B, You Z. Multiplexed Analysis for Anti-Epidermal Growth Factor Receptor Tumor Cell Growth Inhibition Based on Quantum Dot Probes. Anal Chem. 2016;88:4318-27 pubmed publisher
immunocytochemistry; human; 5 ug/ml; loading ...; fig 2a	Rico Varela J, Singh T, McCutcheon S, Vazquez M. EGF as a New Therapeutic Target for Medulloblastoma Metastasis. Cell Mol Bioeng. 2015;8:553-565 pubmed
	Byambaa B, Konno T, Ishihara K. Detachment of cells adhered on the photoreactive phospholipid polymer surface by photoirradiation and their functionality. Colloids Surf B Biointerfaces. 2013;103:489-95 pubmed publisher

product information

Product Type :

Antibody

Product Name :

EGFR Recombinant Rabbit Monoclonal Antibody (30H45L48)

Catalog # :

700308

Quantity :

100µg

Price :

US 345

Clonality :

Recombinant Monoclonal

Purity :

protein A

Host :

Rabbit

Reactivity :

Human

Applications :

Immunocytochemistry: 5 µg/mL, Immunofluorescence: 5 µg/mL, Western Blot: 1:1:000

Species :

Human

Clone :

30H45L48

Isotype :

IgG

Storage :

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Description :

EGFR (Epidermal growth factor receptor, HER1, ErbB1) is encoded by the EGFR gene located on chromosome 7 in humans. EGFR belongs to the HER/ERbB family of proteins that includes three other receptor tyrosine kinases, ERbB2, ERbB3, ERbB4. EGFR is a transmembrane receptor and binding of its cognate ligands such as EGF (Epidermal Growth Factor) and TGF alpha (Transforming Growth Factor alpha) to the extracellular domain leads to EGFR dimerization followed by autophosphorylation of the tyrosine residues in the cytoplasmic domain. Phosphorylation of EGFR at certain residues is also mediated by Src-non-receptor kinase. EGFR activation signals multiple downstream signaling cascades such as the Ras - ERK, PI3-K - Akt, Jak - STAT and PKC pathways that help in growth and proliferation of cells. Phosphorylation of EGFR at Y1086 specifically allows binding of the adaptor protein GRB2, leading to activation of the MAPK pathway. Upon receptor activation and signaling, EGFR is endocytosed and targeted for degradation or recycling. Mutations in the EGFR gene are associated with lung cancer and multiple alternatively spliced transcript variants encode different protein isoforms of EGFR have been found. Increased production or activation of EGFR has been associated with poor prognosis in a variety of tumors. Moreover, EGFR overexpression is observed in tumors of the head and neck, brain, bladder, stomach, breast, lung, endometrium, cervix, vulva, ovary, esophagus, stomach and in squamous cell carcinoma.

Immunogen :

proprietary

Format :

Liquid

Applications w/Dilutions :

Immunocytochemistry: 5 µg/mL, Immunofluorescence: 5 µg/mL, Western Blot: 1:1:000

Aliases :

2.7.10.1; 9030024J15Rik; AI552599; avian erythroblastic leukemia viral (v-erbB) oncogene homolog; avian erythroblastic leukemia viral (v-erb-b) oncogene homolog; cell growth inhibiting protein 40; cell proliferation-inducing protein 61; EC 2.7.10.1; egf receptor; Egfr; EGFR-related peptide; Epidermal growth factor receptor; epidermal growth factor receptor (erythroblastic leukemia viral (v-erb-b) oncogene homolog, avian); Epidermal growth factor receptor formerly avian erythroblastic leukemia viral (v-erbB) oncogene homolog (Erbb1); epidermal growth factor receptor, formerly avian erythroblastic leukemia viral (v-erbB) oncogene homolog (Erbb1); ERBB; ERBB1; ErbB-1; erb-b2 receptor tyrosine kinase 1; Errb1; Errp; HER1; kinase EGFR; mENA; NISBD2; Oncogene ERBB; PIG61; Proto-oncogene c-ErbB-1; Receptor tyrosine-protein kinase erbB-1; Urogastrone; wa2; wa-2; Wa5; waved 2

more info or order :

Invitrogen product webpage