beta Amyloid Recombinant Rabbit Monoclonal Antibody (H31L21)

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

beta Amyloid Recombinant Rabbit Monoclonal Antibody (H31L21)

catalog :

700254

quantity :

100 µg

price :

US 375.00

clonality :

recombinant

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

H31L21

reactivity :

African green monkey, human, mouse

application :

western blot, ELISA, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section, immunohistochemistry - free floating section, dot blot

more info or order :

Invitrogen product webpage

citations: 36

Published Application/Species/Sample/Dilution	Reference
immunohistochemistry - frozen section; African green monkey; 1:2500; loading ...; fig 1d	Edler M, Sherwood C, Meindl R, Hopkins W, Ely J, Erwin J, et al. Aged chimpanzees exhibit pathologic hallmarks of Alzheimer's disease. Neurobiol Aging. 2017;59:107-120 pubmed publisher
western blot; human; loading ...; fig 6b	Rocchi A, Yamamoto S, Ting T, Fan Y, SADLEIR K, Wang Y, et al. A Becn1 mutation mediates hyperactive autophagic sequestration of amyloid oligomers and improved cognition in Alzheimer's disease. PLoS Genet. 2017;13:e1006962 pubmed publisher
immunohistochemistry - free floating section; mouse; 1:200; loading ...; fig st1	Klementieva O, Willén K, Martinsson I, Israelsson B, Engdahl A, Cladera J, et al. Pre-plaque conformational changes in Alzheimer's disease-linked Aβ and APP. Nat Commun. 2017;8:14726 pubmed publisher
ELISA; human; loading ...; fig 1a	Lejbman N, OLIVERA A, Heinzelmann M, Feng R, Yun S, Kim H, et al. Active duty service members who sustain a traumatic brain injury have chronically elevated peripheral concentrations of A?40 and lower ratios of A?42/40. Brain Inj. 2016;30:1436-1441 pubmed
dot blot; mouse; 1:1000; loading ...; fig 6a immunohistochemistry; mouse; 1:500; loading ...; fig 6b	Fan Y, Wang N, Rocchi A, Zhang W, Vassar R, Zhou Y, et al. Identification of natural products with neuronal and metabolic benefits through autophagy induction. Autophagy. 2017;13:41-56 pubmed publisher
immunohistochemistry; mouse; loading ...; fig 2a ELISA; mouse; loading ...; fig 5a western blot; mouse; loading ...; fig 1f	Dinkins M, Enasko J, Hernandez C, Wang G, Kong J, Helwa I, et al. Neutral Sphingomyelinase-2 Deficiency Ameliorates Alzheimer's Disease Pathology and Improves Cognition in the 5XFAD Mouse. J Neurosci. 2016;36:8653-67 pubmed publisher
immunohistochemistry; mouse; loading ...; fig 7e	Badea A, Kane L, Anderson R, Qi Y, Foster M, Cofer G, et al. The fornix provides multiple biomarkers to characterize circuit disruption in a mouse model of Alzheimer's disease. Neuroimage. 2016;142:498-511 pubmed publisher
immunocytochemistry; mouse; 1:600; loading ...; fig 1b	Sadleir K, Kandalepas P, Buggia Prevot V, Nicholson D, Thinakaran G, Vassar R. Presynaptic dystrophic neurites surrounding amyloid plaques are sites of microtubule disruption, BACE1 elevation, and increased Aβ generation in Alzheimer's disease. Acta Neuropathol. 2016;132:235-56 pubmed publisher
immunohistochemistry - free floating section; human; 1:100; tbl 2	Perez S, Sherwood C, Cranfield M, Erwin J, Mudakikwa A, Hof P, et al. Early Alzheimer's disease-type pathology in the frontal cortex of wild mountain gorillas (Gorilla beringei beringei). Neurobiol Aging. 2016;39:195-201 pubmed publisher
immunohistochemistry - frozen section; mouse	Kan M, Lee J, Wilson J, Everhart A, Brown C, Hoofnagle A, et al. Arginine deprivation and immune suppression in a mouse model of Alzheimer's disease. J Neurosci. 2015;35:5969-82 pubmed publisher
immunohistochemistry - paraffin section; human; fig 4	Postupna N, Keene C, Crane P, Gonzalez Cuyar L, Sonnen J, Hewitt J, et al. Cerebral cortical AÎ²42 and PHF-Ï„ in 325 consecutive brain autopsies stratified by diagnosis, location, and APOE. J Neuropathol Exp Neurol. 2015;74:100-9 pubmed publisher
ELISA; mouse; fig 3 western blot; mouse; 1:2500; fig 2	Sadleir K, Eimer W, Cole S, Vassar R. AÎ² reduction in BACE1 heterozygous null 5XFAD mice is associated with transgenic APP level. Mol Neurodegener. 2015;10:1 pubmed publisher
immunocytochemistry; human; 1:2000	Wu Z, Guo Z, Gearing M, Chen G. Tonic inhibition in dentate gyrus impairs long-term potentiation and memory in an Alzheimer's [corrected] disease model. Nat Commun. 2014;5:4159 pubmed publisher
immunohistochemistry - frozen section; mouse; 1:500	Washington P, Morffy N, Parsadanian M, Zapple D, Burns M. Experimental traumatic brain injury induces rapid aggregation and oligomerization of amyloid-beta in an Alzheimer's disease mouse model. J Neurotrauma. 2014;31:125-34 pubmed publisher
immunocytochemistry; human western blot; human	Li W, Tang Y, Fan Z, Meng Y, Yang G, Luo J, et al. Autophagy is involved in oligodendroglial precursor-mediated clearance of amyloid peptide. Mol Neurodegener. 2013;8:27 pubmed publisher
western blot; human; 1:2500	Sadleir K, Bennett D, Schneider J, Vassar R. Elevated A?42 in aged, non-demented individuals with cerebral atherosclerosis. Curr Alzheimer Res. 2013;10:785-9 pubmed
immunohistochemistry - frozen section; mouse; 1:60	Toth M, Szegedi V, Varga E, Juhasz G, Horváth J, Borbély E, et al. Overexpression of Hsp27 ameliorates symptoms of Alzheimer's disease in APP/PS1 mice. Cell Stress Chaperones. 2013;18:759-71 pubmed publisher
immunohistochemistry - free floating section; mouse; 1:1000	Eimer W, Vassar R. Neuron loss in the 5XFAD mouse model of Alzheimer's disease correlates with intraneuronal A?42 accumulation and Caspase-3 activation. Mol Neurodegener. 2013;8:2 pubmed publisher
ELISA; human; 0.1 ug/ml	Zetterberg H, Mörtberg E, Song L, Chang L, Provuncher G, Patel P, et al. Hypoxia due to cardiac arrest induces a time-dependent increase in serum amyloid ? levels in humans. PLoS ONE. 2011;6:e28263 pubmed publisher
	Elsherbini A, Kirov A, Dinkins M, Wang G, Qin H, Zhu Z, et al. Association of Aβ with ceramide-enriched astrosomes mediates Aβ neurotoxicity. Acta Neuropathol Commun. 2020;8:60 pubmed publisher
	Carreras I, Aytan N, Choi J, Tognoni C, Kowall N, Jenkins B, et al. Dual dose-dependent effects of fingolimod in a mouse model of Alzheimer's disease. Sci Rep. 2019;9:10972 pubmed publisher
	Leyns C, Gratuze M, Narasimhan S, Jain N, Koscal L, Jiang H, et al. TREM2 function impedes tau seeding in neuritic plaques. Nat Neurosci. 2019;: pubmed publisher
	More J, Galusso N, Veloso P, Montecinos L, Finkelstein J, Sanchez G, et al. N-Acetylcysteine Prevents the Spatial Memory Deficits and the Redox-Dependent RyR2 Decrease Displayed by an Alzheimer's Disease Rat Model. Front Aging Neurosci. 2018;10:399 pubmed publisher
	Keene C, Wilson A, Kilgore M, Bruner L, Postupna N, Darvas M. Luminex-based quantification of Alzheimer's disease neuropathologic change in formalin-fixed post-mortem human brain tissue. Lab Invest. 2018;: pubmed publisher
	Sadleir K, Popovic J, Vassar R. ER stress is not elevated in the 5XFAD mouse model of Alzheimer's disease. J Biol Chem. 2018;293:18434-18443 pubmed publisher
	Ilievski V, Zuchowska P, Green S, Toth P, Ragozzino M, Le K, et al. Chronic oral application of a periodontal pathogen results in brain inflammation, neurodegeneration and amyloid beta production in wild type mice. PLoS ONE. 2018;13:e0204941 pubmed publisher
	Milanovic D, Petrović S, Brkic M, Avramović V, Perovic M, Ivkovic S, et al. Short-Term Fish Oil Treatment Changes the Composition of Phospholipids While Not Affecting the Expression of Mfsd2a Omega-3 Transporter in the Brain and Liver of the 5xFAD Mouse Model of Alzheimer's Disease. Nutrients. 2018;10: pubmed publisher
	Eimer W, Vijaya Kumar D, Navalpur Shanmugam N, Rodriguez A, Mitchell T, Washicosky K, et al. Alzheimer's Disease-Associated β-Amyloid Is Rapidly Seeded by Herpesviridae to Protect against Brain Infection. Neuron. 2018;99:56-63.e3 pubmed publisher
	Chai Q, Jovasevic V, Malikov V, Sabo Y, MORHAM S, Walsh D, et al. HIV-1 counteracts an innate restriction by amyloid precursor protein resulting in neurodegeneration. Nat Commun. 2017;8:1522 pubmed publisher
	Willén K, Sroka A, Takahashi R, Gouras G. Heterogeneous Association of Alzheimer's Disease-Linked Amyloid-β and Amyloid-β Protein Precursor with Synapses. J Alzheimers Dis. 2017;60:511-524 pubmed publisher
	Willén K, Edgar J, Hasegawa T, Tanaka N, Futter C, Gouras G. A? accumulation causes MVB enlargement and is modelled by dominant negative VPS4A. Mol Neurodegener. 2017;12:61 pubmed publisher
	Ubelmann F, Burrinha T, Salavessa L, Gomes R, Ferreira C, Moreno N, et al. Bin1 and CD2AP polarise the endocytic generation of beta-amyloid. EMBO Rep. 2017;18:102-122 pubmed publisher
	Condello C, Yuan P, Schain A, Grutzendler J. Microglia constitute a barrier that prevents neurotoxic protofibrillar AÎ²42 hotspots around plaques. Nat Commun. 2015;6:6176 pubmed publisher
	Dinkins M, Dasgupta S, Wang G, Zhu G, Bieberich E. Exosome reduction in vivo is associated with lower amyloid plaque load in the 5XFAD mouse model of Alzheimer's disease. Neurobiol Aging. 2014;35:1792-800 pubmed publisher
	Perez S, Raghanti M, Hof P, Kramer L, Ikonomovic M, Lacor P, et al. Alzheimer's disease pathology in the neocortex and hippocampus of the western lowland gorilla (Gorilla gorilla gorilla). J Comp Neurol. 2013;521:4318-38 pubmed publisher
	Zhang Q, Wang R, Scott E, Han D, Dong Y, Tu J, et al. Hypersensitivity of the hippocampal CA3 region to stress-induced neurodegeneration and amyloidogenesis in a rat model of surgical menopause. Brain. 2013;136:1432-45 pubmed publisher

product information

Product Type :

Antibody

Product Name :

beta Amyloid Recombinant Rabbit Monoclonal Antibody (H31L21)

Catalog # :

700254

Quantity :

100 µg

Price :

US 375.00

Clonality :

Recombinant Monoclonal

Purity :

protein A

Host :

Rabbit

Reactivity :

Human, Mouse

Applications :

ELISA: 0.1-0.2 µg/mL, Immunohistochemistry (Paraffin): 1 µg/mL

Species :

Human, Mouse

Clone :

H31L21

Isotype :

IgG

Storage :

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Description :

Amyloid beta peptide (Abeta/Beta-amyloid) is the major constituent of amyloid plaques in the brains of individuals afflicted with Alzheimer's disease. Abeta peptide is 40-43 amino acids long and generated from the beta-amyloid precursor protein (beta APP) in a two-step process. The first step involves cleavage of the extracellular, amino-terminal domain of beta APP. Protein cleavage is performed by an aspartyl protease, beta-secretase (BACE) which is synthesized as a propeptide and must be modified to the mature and active form by the prohormone convertase, furin. Beta APP cleavage by the mature form of BACE results in the cellular secretion of a segment of beta APP, and a membrane-bound remnant. The remnant protein is processed by another protease, gamma-secretase. Gamma-secretase cleaves an intra-membrane site in the carboxyl-terminal domain of beta APP, thus generating the amyloid beta peptide. Gamma-secretase is believed to be a multi-subunit complex containing presenilin-1 and 2 as central components. The transmembrane glycoprotein, nicastrin, is associated with presinilins and has been found to bind to the carboxyl-terminus of beta APP and helps to modulate the production of the amyloid beta peptide. Abeta is an extracellular filamentous protein component of amyloid cores, neuritic plaques and is also found as a deposit in neurofibrillary tangles. Alzheimer’s disease, the most common cause of senile dementia, is characterized by abnormal filamentous protein deposits in the brain. Beta amyloid deposits are also detected in Lewy body dementia, Down’s syndrome, amyloidosis (Dutch type), cerebroarterial amyloidosis (cerebral amyloid angiopathy) and in the Guam Parkinson-Dementia complex.

Immunogen :

A peptide corresponding to amino acids 707-713 of P05067.

Format :

Liquid

Applications w/Dilutions :

ELISA: 0.1-0.2 µg/mL, Immunohistochemistry (Paraffin): 1 µg/mL

Aliases :

A4; AAA; ABETA; Abeta40; Abeta42; ABPP; AD1; Adap; AG; AICD-50; AICD-57; AICD-59; AID(50); AID(57); AID(59); Alpha-CTF; Alpha-secretase C-terminal fragment; Alzheimer disease; Alzheimer disease amyloid A4 protein homolog; alzheimer disease amyloid protein; Amyloid; amyloid A4; Amyloid b; Amyloid beta; amyloid beta (A4) precursor protein; amyloid beta (A4) precursor protein (peptidase nexin-II, Alzheimer disease); amyloid beta A4 protein; amyloid beta precursor protein; Amyloid intracellular domain 50; Amyloid intracellular domain 57; Amyloid intracellular domain 59; Amyloid precursor protein; amyloid precursor protein variant 1; amyloid precursor protein variant 2; Amyloid ß; amyloid-beta A4 protein; amyloid-beta A4 protein; amyloid beta A4 protein; Amyloid-beta precursor protein; Amyloid-beta protein 40; Amyloid-beta protein 42; Amyloidogenic glycoprotein; APP; APP-C57; APP-C59; APP-C99; APPI; appican; beta amyloid protein; beta amyloid protein precursor; beta-amyloid peptide; beta-amyloid peptide(1-40); beta-amyloid peptide(1-42); Beta-amyloid precursor protein; betaApp; Beta-APP40; Beta-APP42; Beta-CTF; Beta-secretase C-terminal fragment; C31; C80; C83; C99; Cerebral vascular amyloid peptide; CTF gamma; CTF-alpha; CTFgamma; CVAP; E030013M08Rik; Gamma-CTF(50); Gamma-CTF(57); Gamma-CTF(59); Gamma-secretase C-terminal fragment 50; Gamma-secretase C-terminal fragment 57; Gamma-secretase C-terminal fragment 59; N-APP; OTTHUMP00000096096; P3(40); P3(42); Pan-Abeta; peptidase nexin-II; PN2; PN-II; PreA4; PreA4 751; protease nexin II; protease nexin-II; S-APP-alpha; S-APP-beta; Soluble APP-alpha; Soluble APP-beta; testicular tissue protein Li 2

more info or order :

Invitrogen product webpage