Cullin 2 Recombinant Rabbit Monoclonal Antibody (50H17L12) | Invitrogen 700179 product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

Cullin 2 Recombinant Rabbit Monoclonal Antibody (50H17L12)

catalog :

700179

quantity :

100 ug

price :

US 471.00

clonality :

recombinant

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

50H17L12

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry, flow cytometry, immunohistochemistry - paraffin section

more info or order :

Invitrogen product webpage

citations: 4

Published Application/Species/Sample/Dilution	Reference
western blot; human; 1:5000; loading ...; fig 4b	Smith B, Wang S, Jaime Figueroa S, Harbin A, Wang J, Hamman B, et al. Differential PROTAC substrate specificity dictated by orientation of recruited E3 ligase. Nat Commun. 2019;10:131 pubmed publisher
	Keuss M, Hjerpe R, Hsia O, Gourlay R, Burchmore R, Trost M, et al. Unanchored tri-NEDD8 inhibits PARP-1 to protect from oxidative stress-induced cell death. EMBO J. 2019;38: pubmed publisher
	Westrich J, Warren C, Klausner M, Guo K, Liu C, Santiago M, et al. Human Papillomavirus 16 E7 Stabilizes APOBEC3A Protein by Inhibiting Cullin 2-Dependent Protein Degradation. J Virol. 2018;92: pubmed publisher
	Keuss M, Thomas Y, Mcarthur R, Wood N, Knebel A, Kurz T. Characterization of the mammalian family of DCN-type NEDD8 E3 ligases. J Cell Sci. 2016;129:1441-54 pubmed publisher

product information

Product Type :

Antibody

Product Name :

Cullin 2 Recombinant Rabbit Monoclonal Antibody (50H17L12)

Catalog # :

700179

Quantity :

100 ug

Price :

US 471.00

Clonality :

Recombinant Monoclonal

Purity :

protein A

Host :

Rabbit

Reactivity :

Human, Mouse, Rat

Applications :

Flow Cytometry: 0.5-1 ug/test, Immunocytochemistry: 8-12 ug/mL, Immunohistochemistry (Paraffin): 4-6 ug/mL, Western Blot: 3-5 ug/mL

Species :

Human, Mouse, Rat

Clone :

50H17L12

Isotype :

IgG

Storage :

Store at 4 C short term. For long term storage, store at -20 C, avoiding freeze/thaw cycles.

Description :

Core component of multiple cullin-RING-based ECS (ElonginB/C-CUL2/5-SOCS-box protein) E3 ubiquitin-protein ligase complexes, which mediate the ubiquitination of target proteins. ECS complexes and ARIH1 collaborate in tandem to mediate ubiquitination of target proteins (PubMed:27565346). May serve as a rigid scaffold in the complex and may contribute to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme. The E3 ubiquitin-protein ligase activity of the complex is dependent on the neddylation of the cullin subunit and is inhibited by the association of the deneddylated cullin subunit with TIP120A/CAND1. The functional specificity of the ECS complex depends on the substrate recognition component. ECS(VHL) mediates the ubiquitination of hypoxia-inducible factor (HIF). [UniProt]

Immunogen :

A peptide corresponding to amino acids 733-745 of Q13617.

Format :

Liquid

Applications w/Dilutions :

Flow Cytometry: 0.5-1 ug/test, Immunocytochemistry: 8-12 ug/mL, Immunohistochemistry (Paraffin): 4-6 ug/mL, Western Blot: 3-5 ug/mL

Aliases :

1300003D18Rik; 4932411N15Rik; AI327301; CUL2; CUL-2; cullin 2; cullin-2; MGC131970; mKIAA4106; OTTHUMP00000019437; testis secretory sperm-binding protein Li 238E

more info or order :

Invitrogen product webpage