Vinculin Recombinant Rabbit Monoclonal Antibody (42H89L44) | Invitrogen 700062 product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

Vinculin Recombinant Rabbit Monoclonal Antibody (42H89L44)

catalog :

700062

quantity :

100 µg

price :

US 425.00

clonality :

recombinant

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

42H89L44

reactivity :

African green monkey, human, mouse

application :

western blot, immunocytochemistry, flow cytometry

more info or order :

Invitrogen product webpage

citations: 19

Published Application/Species/Sample/Dilution	Reference
immunocytochemistry; human; loading ...; fig 3b	Gong T, Heng B, Xu J, Zhu S, Yuan C, Lo E, et al. Decellularized extracellular matrix of human umbilical vein endothelial cells promotes endothelial differentiation of stem cells from exfoliated deciduous teeth. J Biomed Mater Res A. 2017;105:1083-1093 pubmed publisher
immunocytochemistry; human; fig 2a	Gallego Delgado J, Basu Roy U, Ty M, Alique M, Fernandez Arias C, Movila A, et al. Angiotensin receptors and ?-catenin regulate brain endothelial integrity in malaria. J Clin Invest. 2016;126:4016-4029 pubmed publisher
immunocytochemistry; human; loading ...; fig 1a immunocytochemistry; African green monkey; loading ...; fig 1b	Advani A, Chen A, Babbitt C. Human fibroblasts display a differential focal adhesion phenotype relative to chimpanzee. Evol Med Public Health. 2016;2016:110-6 pubmed publisher
immunocytochemistry; human; 1:200; fig 4	Heng B, Zhu S, Xu J, Yuan C, Gong T, Zhang C. Effects of decellularized matrices derived from periodontal ligament stem cells and SHED on the adhesion, proliferation and osteogenic differentiation of human dental pulp stem cells in vitro. Tissue Cell. 2016;48:133-43 pubmed publisher
immunocytochemistry; human; 1:200; loading ...; fig s3d	Brum A, van de Peppel J, van der Leije C, Schreuders Koedam M, Eijken M, van der Eerden B, et al. Connectivity Map-based discovery of parbendazole reveals targetable human osteogenic pathway. Proc Natl Acad Sci U S A. 2015;112:12711-6 pubmed publisher
immunocytochemistry; human	Richter E, Harms M, Ventz K, Gierok P, Chilukoti R, Hildebrandt J, et al. A multi-omics approach identifies key hubs associated with cell type-specific responses of airway epithelial cells to staphylococcal alpha-toxin. PLoS ONE. 2015;10:e0122089 pubmed publisher
immunocytochemistry; human; fig 3	CURTIS J, Luo Y, Zenner H, Cuchet LourenÃ§o D, Wu C, Lo K, et al. Susceptibility to tuberculosis is associated with variants in the ASAP1 gene encoding a regulator of dendritic cell migration. Nat Genet. 2015;47:523-527 pubmed publisher
immunocytochemistry; human	Rubashkin M, Cassereau L, Bainer R, DuFort C, Yui Y, Ou G, et al. Force engages vinculin and promotes tumor progression by enhancing PI3K activation of phosphatidylinositol (3,4,5)-triphosphate. Cancer Res. 2014;74:4597-611 pubmed publisher
	Betriu N, Jarrosson Moral C, Semino C. Culture and Differentiation of Human Hair Follicle Dermal Papilla Cells in a Soft 3D Self-Assembling Peptide Scaffold. Biomolecules. 2020;10: pubmed publisher
	Liu Y, Xiao J, Zhang B, Shelite T, Su Z, Chang Q, et al. Increased talin-vinculin spatial proximities in livers in response to spotted fever group rickettsial and Ebola virus infections. Lab Invest. 2020;100:1030-1041 pubmed publisher
	Gupte T, Ritt M, Dysthe M, Malik R, Sivaramakrishnan S. Minute-scale persistence of a GPCR conformation state triggered by non-cognate G protein interactions primes signaling. Nat Commun. 2019;10:4836 pubmed publisher
	Sosnowska M, Kutwin M, Jaworski S, Strojny B, Wierzbicki M, Szczepaniak J, et al. Mechano-signalling, induced by fullerene C60 nanofilms, arrests the cell cycle in the G2/M phase and decreases proliferation of liver cancer cells. Int J Nanomedicine. 2019;14:6197-6215 pubmed publisher
	Chen Y, Huang B, Lee W, Chen Y. 16-Hydroxycleroda-3,13-dien-15,16-olide induces anoikis in human renal cell carcinoma cells: involvement of focal adhesion disassembly and signaling. Onco Targets Ther. 2018;11:7679-7690 pubmed publisher
	Noethel B, Ramms L, Dreissen G, Hoffmann M, Springer R, Rübsam M, et al. Transition of responsive mechanosensitive elements from focal adhesions to adherens junctions on epithelial differentiation. Mol Biol Cell. 2018;29:2317-2325 pubmed publisher
	Wierzbicki M, Jaworski S, Kutwin M, Grodzik M, Strojny B, Kurantowicz N, et al. Diamond, graphite, and graphene oxide nanoparticles decrease migration and invasiveness in glioblastoma cell lines by impairing extracellular adhesion. Int J Nanomedicine. 2017;12:7241-7254 pubmed publisher
	Gentile P, McColgan Bannon K, Gianone N, Sefat F, Dalgarno K, Ferreira A. Biosynthetic PCL-graft-Collagen Bulk Material for Tissue Engineering Applications. Materials (Basel). 2017;10: pubmed publisher
	Patterson D, Roberts J, King V, Houserova D, Barnhill E, Crucello A, et al. Human snoRNA-93 is processed into a microRNA-like RNA that promotes breast cancer cell invasion. NPJ Breast Cancer. 2017;3:25 pubmed publisher
	Mekhdjian A, Kai F, Rubashkin M, Prahl L, Przybyla L, McGregor A, et al. Integrin-mediated traction force enhances paxillin molecular associations and adhesion dynamics that increase the invasiveness of tumor cells into a three-dimensional extracellular matrix. Mol Biol Cell. 2017;28:1467-1488 pubmed publisher
	Xu K, Babcock H, Zhuang X. Dual-objective STORM reveals three-dimensional filament organization in the actin cytoskeleton. Nat Methods. 2012;9:185-8 pubmed publisher

product information

Product Type :

Antibody

Product Name :

Vinculin Recombinant Rabbit Monoclonal Antibody (42H89L44)

Catalog # :

700062

Quantity :

100 µg

Price :

US 425.00

Clonality :

Recombinant Monoclonal

Purity :

protein A

Host :

Rabbit

Reactivity :

Human, Mouse

Applications :

Flow Cytometry: 2-4 µg/1x10^6 cells, Immunocytochemistry: 0.5-5 µg/mL, Western Blot: 0.5-3 µg/mL

Species :

Human, Mouse

Clone :

42H89L44

Isotype :

IgG

Storage :

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Description :

Vinculin (VCL) is a cytoskeletal protein associated with cell-cell and cell-extracellular matrix adherens-type junctions. It functions as one of several interacting proteins involved in anchoring F-actin to the membrane. It has been shown that a sequence of molecular interactions might be involved in the transmembrane assembly of adhesion plaques. In the assembly of adhesion plaques, the beta subunit of integrin binds to talin. Talin binds to vinculin that interacts with alpha-actinin and possibly with itself. Since alpha-actinin binds to and cross-links actin filaments, vinculin represents a key element in the transmembrane linkage of the extracellular matrix to the cytoplasmic microfilament system. Mutations in the gene can result in cardiomyopathy dilated 1W or familial hypertrophic 15.

Immunogen :

A recombinant protein corresponding to amino acids 408-591 of P18206.

Format :

Liquid

Applications w/Dilutions :

Flow Cytometry: 2-4 µg/1x10^6 cells, Immunocytochemistry: 0.5-5 µg/mL, Western Blot: 0.5-3 µg/mL

Aliases :

9430097D22; AA571387; AI462105; AW545629; CMD1W; CMH15; epididymis luminal protein 114; HEL114; metavinculin; meta-vinculin; metavinculin {ECO:0000250; MV; MVCL; RP11-178G16.3; UniProtKB:P18206}; unnamed protein product; Vcl; vcl {ECO:0000250; VIN; vinc; vinc 1; VINC1; Vinculin; vinculin {ECO:0000250

more info or order :

Invitrogen product webpage