SMAD2 Recombinant Rabbit Monoclonal Antibody (31H15L4) | Invitrogen 700048 product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

SMAD2 Recombinant Rabbit Monoclonal Antibody (31H15L4)

catalog :

700048

quantity :

100 µg

price :

US 500.00

clonality :

recombinant

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

31H15L4

reactivity :

human, mouse

application :

western blot, ELISA, immunocytochemistry, flow cytometry, immunohistochemistry - paraffin section

more info or order :

Invitrogen product webpage

citations: 6

Published Application/Species/Sample/Dilution	Reference
immunohistochemistry - paraffin section; mouse; 1:100; fig 6	Terakawa J, Rocchi A, Serna V, Bottinger E, Graff J, Kurita T. FGFR2IIIb-MAPK Activity Is Required for Epithelial Cell Fate Decision in the Lower Müllerian Duct. Mol Endocrinol. 2016;30:783-95 pubmed publisher
western blot; human; 1:2500; fig 4	Xu X, Tan X, Hulshoff M, Wilhelmi T, Zeisberg M, Zeisberg E. Hypoxia-induced endothelial-mesenchymal transition is associated with RASAL1 promoter hypermethylation in human coronary endothelial cells. FEBS Lett. 2016;590:1222-33 pubmed publisher
	Luo Y, Yan B, Liu L, Yin L, Ji H, An X, et al. Sulforaphane Inhibits the Expression of Long Noncoding RNA H19 and Its Target APOBEC3G and Thereby Pancreatic Cancer Progression. Cancers (Basel). 2021;13: pubmed publisher
	Lin L, Kost E, Lin C, Valente P, Wang C, Kolonin M, et al. PAI-1-Dependent Inactivation of SMAD4-Modulated Junction and Adhesion Complex in Obese Endometrial Cancer. Cell Rep. 2020;33:108253 pubmed publisher
	Gao Y, Yang H, Wang Y, Tian J, Li R, Zhou X. Evaluation of the inhibitory effect of tacrolimus combined with mycophenolate mofetil on mesangial cell proliferation based on the cell cycle. Int J Mol Med. 2020;46:1582-1592 pubmed publisher
	Van Boxtel A, Economou A, Heliot C, Hill C. Long-Range Signaling Activation and Local Inhibition Separate the Mesoderm and Endoderm Lineages. Dev Cell. 2018;44:179-191.e5 pubmed publisher

product information

Product Type :

Antibody

Product Name :

SMAD2 Recombinant Rabbit Monoclonal Antibody (31H15L4)

Catalog # :

700048

Quantity :

100 µg

Price :

US 500.00

Clonality :

Recombinant Monoclonal

Purity :

protein A

Host :

Rabbit

Reactivity :

Human

Applications :

ChIP Assay: 3 µg, ELISA: 1-5 µg/mL, Flow Cytometry: 1-3 µg/1x10^6 cells, Immunocytochemistry: 1 µg/mL, Western Blot: 0.5-1 µg/mL

Species :

Human

Clone :

31H15L4

Isotype :

IgG

Storage :

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Description :

SMAD2 (MADH2, MAD2) regulates multiple cellular processes, such as cell proliferation, apoptosis, and differentiation. SMAD2 interacts with the TGF-beta receptors through its interaction with the SMAD anchor for receptor activation into the nucleus is a central event in TGF beta signaling. Phosphorylation of threonine 8 in the calmodulin-binding region of the MH1 domain by extracellular signal regulated kinase 1 (ERK1) enhances SMAD2 transcriptional activity, which is negatively regulated by calmodulin. In response to the TGF-beta signal, SMAD2 is phosphorylated by the TGF-beta receptors. The phosphorylation induces the dissociation of this protein with SARA and the association with the family member SMAD4. The association with SMAD4 is important for the translocation of this protein into the nucleus, where it binds to target promoters and forms a transcription repressor complex with other cofactors. SMAD2 can also be phosphorylated by activin type 1 receptor kinase, and mediates the signal from the activin. Alternatively spliced transcript variants encoding the SMAD2 protein have been observed.

Immunogen :

A peptide corresponding to amino acids 81-107 of Q15796.

Format :

Liquid

Applications w/Dilutions :

ChIP Assay: 3 µg, ELISA: 1-5 µg/mL, Flow Cytometry: 1-3 µg/1x10^6 cells, Immunocytochemistry: 1 µg/mL, Western Blot: 0.5-1 µg/mL

Aliases :

7120426M23Rik; EGK_09664; hMAD-2; hSMAD2; JV18; JV18-1; MAD; MAD homolog 2; Mad protein homolog; Madh2; Madr2; Mad-related protein 2; MGC22139; MGC34440; mMad2; mother against DPP homolog 2; mothers against decapentaplegic homolog 2; Mothers against decapentaplegic-like protein 2; mothers against DPP homolog 2; pSMAD2; Sma- and Mad-related protein 2; SMAD; SMAD 2; SMAD family member 2; SMAD, mothers against DPP homolog 2; Smad2; Smad-2; Smad2Deltaexon3

more info or order :

Invitrogen product webpage