Phospho-SMAD1/SMAD5 (Ser463, Ser465) Recombinant Rabbit Monoclonal Antibody (31H14L11)

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

Phospho-SMAD1/SMAD5 (Ser463, Ser465) Recombinant Rabbit Monoclonal Antibody (31H14L11)

catalog :

700047

quantity :

100 µg

price :

US 375.00

clonality :

recombinant

host :

domestic rabbit

conjugate :

nonconjugated

antigen modification :

phosphorylated

clone name :

31H14L11

reactivity :

human, mouse

application :

western blot, immunohistochemistry, immunocytochemistry, flow cytometry, immunohistochemistry - paraffin section

more info or order :

Invitrogen product webpage

citations: 5

Published Application/Species/Sample/Dilution	Reference
immunohistochemistry; mouse; loading ...; fig 1n	Iyer S, Chhabra Y, Harvey T, Wang R, Chiu H, Smith A, et al. CRIM1 is necessary for coronary vascular endothelial cell development and homeostasis. J Mol Histol. 2017;48:53-61 pubmed publisher
immunocytochemistry; mouse; fig 6	Iyer S, Chou F, Wang R, Chiu H, Raju V, Little M, et al. Crim1 has cell-autonomous and paracrine roles during embryonic heart development. Sci Rep. 2016;6:19832 pubmed publisher
western blot; mouse; 1:200	Wainwright E, Wilhelm D, Combes A, Little M, Koopman P. ROBO2 restricts the nephrogenic field and regulates Wolffian duct-nephrogenic cord separation. Dev Biol. 2015;404:88-102 pubmed publisher
	Dudakovic A, Camilleri E, Riester S, Paradise C, Gluscevic M, O Toole T, et al. Enhancer of Zeste Homolog 2 Inhibition Stimulates Bone Formation and Mitigates Bone Loss Caused by Ovariectomy in Skeletally Mature Mice. J Biol Chem. 2016;291:24594-24606 pubmed
	Kozmikova I, Candiani S, Fabian P, Gurska D, Kozmik Z. Essential role of Bmp signaling and its positive feedback loop in the early cell fate evolution of chordates. Dev Biol. 2013;382:538-54 pubmed publisher

product information

Product Type :

Antibody

Product Name :

Phospho-SMAD1/SMAD5 (Ser463, Ser465) Recombinant Rabbit Monoclonal Antibody (31H14L11)

Catalog # :

700047

Quantity :

100 µg

Price :

US 375.00

Clonality :

Recombinant Monoclonal

Purity :

protein A

Host :

Rabbit

Reactivity :

Human

Applications :

Flow Cytometry: 0.5-5 µg/1x10^6 cells, Immunocytochemistry: 2-4 µg/mL, Immunohistochemistry (Paraffin): 4-6 µg/mL, Western Blot: 1-2 µg/mL

Species :

Human

Clone :

31H14L11

Isotype :

IgG

Storage :

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Description :

The Smad family of proteins are functioning in the transmission of extracellular signals in the TGF-beta signaling pathway. Binding of a TGF-beta superfamily ligands to extracellular receptors triggers phosphorylation of Smad2 at a Serine-Serine-Methionine-Serine (SSMS) motif at its C-terminus. Phosphorylated Smad2 is then able to form a complex with Smad4. These complexes accumulate in the cell nucleus, where they are directly participating in the regulation of gene expression. In mammals, eight Smad proteins have been identified to date. The Smad family of proteins can be divided into three functional groups: the receptor-activated Smads (R-Smads), common mediator Smads (Co-Smads), and the inhibitory Smads (I-Smads). The R-Smads are directly phosphorylated by the activated type I receptors on their C-terminal Ser-Ser-X-Ser (SSXS) motif and include Smad1, Smad2, Smad3, Smad5, and Smad8. Smad2 and Smad3 are phosphorylated in response to TGF-beta and activin, whereas Smad1, Smad5, and Smad8 are phosphorylated in response to BMP (Bone Morphogenetic Protein). This C-terminal phosphorylation allows R-Smad binding to Co-Smad, Smad4, and translocation to the nucleus where they regulate TGF-beta target genes. Smad6 and Smad7 belong to the I-Smad which bind to the type I receptor or Smad4 and block their interaction with R-Smads.

Immunogen :

A peptide corresponding to amino acids 456-465 of Q15797 and Q99717.

Format :

Liquid

Applications w/Dilutions :

Flow Cytometry: 0.5-5 µg/1x10^6 cells, Immunocytochemistry: 2-4 µg/mL, Immunohistochemistry (Paraffin): 4-6 µg/mL, Western Blot: 1-2 µg/mL

Aliases :

1110051M15Rik; AI451355; AI528653; BSP1; BSP-1; dwarfin-A; Dwarfin-C; dwf-A; DWFC; Dwf-C; hSMAD1; hSmad5; JV41; JV4-1; JV5-1; MAD (mothers against decapentaplegic, Drosophila) homolog 1; MAD homolog 1; MAD homolog 5; MAD homolog1 (mothers against decapentaplegic, Drosophila); MAD, mothers against decapentaplegic homolog 1; MAD, mothers against decapentaplegic homolog 5; Madh1; Madh5; Madr1; mad-related protein 1; Mlp1; mMad1; mothers against decapentaplegic homolog 1; Mothers against decapentaplegic homolog 5; mothers against decapentaplegic, drosophila, homolog of, 5; mothers against DPP homolog 1; mothers against DPP homolog 5; mothers-against-DPP-related 1; Msmad5; MusMLP; OTTHUMP00000223331; SMA- and MAD-related protein 5; SMAD; Smad 1; SMAD 5; SMAD family member 1; SMAD family member 5; SMAD, mothers against DPP homolog 1; SMAD, mothers against DPP homolog 5; SMAD1; SMAD5; TGF-beta signaling protein 1; transforming growth factor-beta signaling protein 1; Transforming growth factor-beta-signaling protein 1

more info or order :

Invitrogen product webpage