Phospho-FAK (Tyr576) Recombinant Rabbit Monoclonal Antibody (2H74L24)

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

Phospho-FAK (Tyr576) Recombinant Rabbit Monoclonal Antibody (2H74L24)

catalog :

700013

quantity :

100 ug

price :

US 520.00

clonality :

recombinant

host :

domestic rabbit

conjugate :

nonconjugated

antigen modification :

phosphorylated

clone name :

2H74L24

reactivity :

human, mouse, zebrafish

application :

western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

more info or order :

Invitrogen product webpage

citations: 7

Published Application/Species/Sample/Dilution	Reference
immunocytochemistry; human; 1:200; fig 8	Petridou N, Skourides P. A ligand-independent integrin Î²1 mechanosensory complex guides spindle orientation. Nat Commun. 2016;7:10899 pubmed publisher
western blot; human; loading ...; fig 2a,2c	Min H, Yun H, Lee J, Lee H, Cho J, Jang H, et al. Targeting the insulin-like growth factor receptor and Src signaling network for the treatment of non-small cell lung cancer. Mol Cancer. 2015;14:113 pubmed publisher
western blot; mouse	McGinnis L, Pelech S, Kinsey W. Post-ovulatory aging of oocytes disrupts kinase signaling pathways and lysosome biogenesis. Mol Reprod Dev. 2014;81:928-45 pubmed publisher
western blot; zebrafish ; 1:500; fig 3	Petridou N, Stylianou P, Christodoulou N, Rhoads D, Guan J, Skourides P. Activation of endogenous FAK via expression of its amino terminal domain in Xenopus embryos. PLoS ONE. 2012;7:e42577 pubmed publisher
	Brudvig J, Cain J, Schmidt Grimminger G, Stumpo D, Roux K, Blackshear P, et al. MARCKS Is Necessary for Netrin-DCC Signaling and Corpus Callosum Formation. Mol Neurobiol. 2018;55:8388-8402 pubmed publisher
	Yoon H, Dehart J, Murphy J, Lim S. Understanding the roles of FAK in cancer: inhibitors, genetic models, and new insights. J Histochem Cytochem. 2015;63:114-28 pubmed publisher
	Petridou N, Skourides P. FAK transduces extracellular forces that orient the mitotic spindle and control tissue morphogenesis. Nat Commun. 2014;5:5240 pubmed publisher

product information

Product Type :

Antibody

Product Name :

Phospho-FAK (Tyr576) Recombinant Rabbit Monoclonal Antibody (2H74L24)

Catalog # :

700013

Quantity :

100 ug

Price :

US 520.00

Clonality :

Recombinant Monoclonal

Purity :

protein A

Host :

Rabbit

Reactivity :

Human

Applications :

Immunocytochemistry: 1:100, Immunohistochemistry (Paraffin): 1:10-1:100

Species :

Human

Clone :

2H74L24

Isotype :

IgG

Storage :

Store at 4 C short term. For long term storage, store at -20 C, avoiding freeze/thaw cycles.

Description :

Focal Adhesion Kinase (FAK) is a 125 kDa non-receptor protein tyrosine kinase that acts as a substrate for Src and is a key element of integrin signaling. FAK plays an important role in cell spreading, differentiation, migration, cell death, and acceleration of the G1 to S phase transition of the cell cycle. FAK has a central catalytic domain and a C-terminal tail that localizes it to focal adhesions, which are sites where cells attach to the extracellular matrix via surface integrin receptors. Increased FAK tyrosine phosphorylation occurs upon integrin engagement with fibronectin. Adhesion of murine NIH3T3 fibroblasts to fibronectin promotes association of the Grb2 adapter protein and c-Src PTK with FAK in vivo, and also results in activation of the ERK2 MAP kinase. In v-Src-transformed NIH3T3, the association of v-Src, Grb2, and Sos with FAK is independent of cell adhesion to fibronectin. In vitro the Grb2 SH2 domain binds directly to tyrosine-phosphorylated FAK, and the binding site has been identified as Tyr925 by site directed mutagenesis. Several transcript variants encoding different isoforms have been found for the FAK gene, but the full-length natures of only three of them have been determined.

Immunogen :

A peptide corresponding to amino acids 573-582 of Q05397.

Format :

Liquid

Applications w/Dilutions :

Immunocytochemistry: 1:100, Immunohistochemistry (Paraffin): 1:10-1:100

Aliases :

CG10023; CG10023-PA; CG10023-PB; CG10023-PC; CG10023-PD; CG10023-PF; CG10023-PG; CT28129; DFAK; DFak56; Dmel CG10023; Dmel_CG10023; DmFAK; EC 2.7.10.2; Fadk; FADK 1; Fak; Fak1; Fak56; fak56D; FAK65D; Fak-PA; Fak-PB; Fak-PC; Fak-PD; Fak-PF; Fak-PG; FAK-related non-kinase polypeptide; focal adhesion kinase; Focal adhesion kinase 1; focal adhesion kinase homolog; focal adhesion kinase isoform FAK-Del33; focal adhesion kinase pp125FAK; focal adhesion kinase-related nonkinase; focal ashension kinase 1; FRNK; I79_019430; Kiaa4203; mKIAA4203; p125FAK; p41/p43FRNK; pFAK; pp125 PTK2; pp125FAK; PPP1R71; Protein phosphatase 1 regulatory subunit 71; protein phosphatase 1, regulatory subunit 71; protein tyrosine kinase 2; protein tyrosine kinase 2 L homeolog; protein-tyrosine kinase; protein-tyrosine kinase 2; PTK2; PTK2 protein tyrosine kinase 2; ptk2.L; tyrosine kinase; XELAEV_18032408mg; XPFAK

more info or order :

Invitrogen product webpage