Histone H3 Polyclonal Antibody
Invitrogen
catalog: PA5-16183
domestic rabbit polyclonal
reactivity: Chlamydomonas reinhardtii, human, mouse, rat
application: western blot, immunocytochemistry
citations: 22
quantity: 100 uL
price: US 514.00
to the supplier
domestic rabbit polyclonal
reactivity: human, mouse, rat, fruit fly , Xenopus laevis
modification: phosphorylated
application: western blot, immunohistochemistry, immunocytochemistry, flow cytometry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section
citations: 29

Immunofluorescent analysis of Phospho-Histone H3 pSer10 (green) in HeLa cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA (Product # 37525) for 15 minutes at room temperature. Cells were probed without (left panel) or with (right panel) a Phospho-Histone H3 polyclonal antibody (Product # PA5-17869) at a dilution of 1:100 for at least 1 hour at room temperature, washed with PBS, and incubated with DyLight 488 goat anti-rabbit IgG secondary antibody (Product # 35552) at a dilution of 1:400 for 30 minutes at room temperature. F-Actin (red) was stained with DyLight 554 Phalloidin (Product # 21834) and nuclei (blue) were stained with Hoechst 33342 dye (Product # 62249). Images were taken on a Thermo Scientific ArrayScan or a ToxInsight Instrument at 20X magnification.

Immunohistochemistry was performed on paraffin-embedded human breast carcinoma. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH 6.0) buffer for 10 minutes at 95C. Following antigen-retrieval, tissues were incubated in 3% hydrogen peroxide for 10 minutes, washed, and then blocked in 5% normal goat serum in TBST for 1 hour at room temperature. Tissues were stained with a Phospho-Histone H3 polyclonal antibody (Product # PA5-17869) at a dilution of 1:200 overnight at 4C. Tissues were washed extensively with TBS + 0.1% Tween-20 and detection was performed using a HRP-conjugated secondary reagent followed by DAB substrate.

Flow cytometric analysis of Phospho-Histone H3 pSer10 on Ramos cells left untreated (blue histogram) or treated with serum/calyculin (green histogram). Cells were harvested, fixed with formaldehyde, permeabilized with methanol, and incubated with a Phospho-Histone H3 pSer10 polyclonal antibody (Product # PA5-17869) at a dilution of 1:50 for 1 hour at room temperature. As a negative control, serum/calyculin-treated cells were stained with an isotype control (red histogram). Following staining with either PA5-17869 or isotype control, cells were stained with a fluorescently-conjugated anti-rabbit IgG secondary antibody and analyzed on a flow cytometer.
quantity: 100 uL
price: US 514.00
to the supplier
Histone H3 Monoclonal Antibody (E.960.2)
Invitrogen
catalog: MA5-15150
domestic rabbit monoclonal (E.960.2)
reactivity: human, mouse, rat, dogs, bovine, zebrafish , fruit fly
application: western blot
citations: 5
quantity: 100 uL
price: US 464.00
to the supplier
mouse monoclonal (K.872.3)
reactivity: human, mouse, rat
modification: phosphorylated
application: western blot, immunohistochemistry, immunocytochemistry, flow cytometry, immunohistochemistry - frozen section
citations: 4
quantity: 100 uL
price: US 470.00
to the supplier
Histone H3 Polyclonal Antibody
Invitrogen
catalog: PA5-31954
domestic rabbit polyclonal
reactivity: human, mouse, rat, fruit fly
application: western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunohistochemistry - paraffin section
citations: 4
domestic rabbit recombinant (17H2L9)
reactivity: human, rat
application: western blot, immunocytochemistry
citations: 2
quantity: 100 ug
price: US 471.00
to the supplier
domestic rabbit recombinant (24HC2 LC12)
reactivity: human, mouse, rat
application: western blot, ELISA, immunocytochemistry
citations: 1
quantity: 100 ug
price: US 520.00
to the supplier
domestic rabbit monoclonal (17HCLC)
reactivity: human, rat
application: western blot, immunocytochemistry
citations: 1
quantity: 100 ug
price: US 520.00
to the supplier
Histone H3 Monoclonal Antibody (6D3B9)
Invitrogen
catalog: MA5-31759
mouse monoclonal (6D3B9)
reactivity: human, mouse, rat
application: western blot, ELISA, flow cytometry
citations: 1
quantity: 100 uL
price: US 480.00
to the supplier
domestic rabbit polyclonal
reactivity: human, mouse, rat, zebrafish
application: western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunohistochemistry - paraffin section
citations: 1
quantity: 100 uL
price: US 454.00
to the supplier
domestic rabbit recombinant (24HC2 LC12)
reactivity: human, mouse, rat
application: western blot, ELISA, immunocytochemistry
citations: 1
quantity: 20 ug
price: US 187.00
to the supplier
H3K4me2 Monoclonal Antibody (T.767.5)
Invitrogen
catalog: MA5-14977
domestic rabbit monoclonal (T.767.5)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunohistochemistry - paraffin section


quantity: 100 uL
price: US 474.00
to the supplier
domestic rabbit polyclonal
reactivity: human, mouse, rat
modification: phosphorylated
application: western blot, immunocytochemistry, immunoprecipitation, flow cytometry



quantity: 100 uL
price: US 464.00
to the supplier
domestic rabbit polyclonal
reactivity: human, mouse, rat
modification: phosphorylated
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section
quantity: 100 uL
price: US 464.00
to the supplier
domestic rabbit monoclonal (24 HCLC)
reactivity: human, mouse, rat
application: western blot, ELISA, immunocytochemistry
quantity: 100 ug
price: US 520.00
to the supplier
domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot
quantity: 100 uL
price: US 464.00
to the supplier
domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot
quantity: 100 uL
price: US 514.00
to the supplier
domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot
quantity: 100 uL
price: US 454.00
to the supplier
domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, immunocytochemistry
quantity: 100 uL
price: US 522.00
to the supplier
domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot
quantity: 100 uL
price: US 522.00
to the supplier
domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot
quantity: 100 uL
price: US 454.00
to the supplier
domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot
quantity: 100 uL
price: US 464.00
to the supplier
domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot
quantity: 100 uL
price: US 522.00
to the supplier
domestic rabbit monoclonal (2HCLC)
reactivity: human, mouse, rat
application: western blot, ELISA, immunocytochemistry
quantity: 100 ug
price: US 520.00
to the supplier
Histone H3 Polyclonal Antibody
Invitrogen
catalog: PA5-49579
domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, immunocytochemistry
quantity: 100 uL
price: US 514.00
to the supplier
domestic rabbit recombinant (RM186)
reactivity: human, mouse, rat
application: western blot, ELISA, immunocytochemistry
quantity: 100 ug
price: US 414.00
to the supplier
domestic rabbit recombinant (17H2L9)
reactivity: human, mouse, rat
conjugate: AF488
application: western blot, immunocytochemistry
quantity: 50 uL
price: US 399.00
to the supplier
domestic rabbit recombinant (17H2L9)
reactivity: human, mouse, rat
conjugate: Alexa Fluor 555
application: western blot, immunocytochemistry
quantity: 50 uL
price: US 414.00
to the supplier
mouse monoclonal (GT921)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section
quantity: 100 uL
price: US 464.00
to the supplier
Histone H3 Polyclonal Antibody
Invitrogen
catalog: PA5-85582
domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section
quantity: 100 uL
price: US 464.00
to the supplier
domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot
quantity: 100 uL
price: US 464.00
to the supplier
H3R17me1 Polyclonal Antibody
Invitrogen
catalog: PA5-91944
domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, ELISA, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section
quantity: 100 uL
price: US 464.00
to the supplier
H3R17me2a Polyclonal Antibody
Invitrogen
catalog: PA5-96125
domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, ELISA, immunocytochemistry
quantity: 100 uL
price: US 454.00
to the supplier
H3K9me1 Polyclonal Antibody
Invitrogen
catalog: PA5-96114
domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, ELISA, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section, dot blot
quantity: 100 uL
price: US 464.00
to the supplier
H3K4me2 Polyclonal Antibody
Invitrogen
catalog: PA5-96113
domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, ELISA, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section
quantity: 100 uL
price: US 464.00
to the supplier
domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, ELISA
quantity: 100 uL
price: US 464.00
to the supplier
domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, ELISA, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section
quantity: 100 uL
price: US 464.00
to the supplier
domestic rabbit recombinant (ARC1662)
reactivity: human, mouse, rat
application: western blot, ELISA
quantity: 100 uL
price: US 491.00
to the supplier
domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section
quantity: 100 uL
price: US 470.00
to the supplier
domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section
quantity: 100 uL
price: US 464.00
to the supplier
domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section
quantity: 100 uL
price: US 450.00
to the supplier
domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, ELISA, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section
quantity: 100 uL
price: US 464.00
to the supplier
Histone H3 Polyclonal Antibody
Invitrogen
catalog: PA5-99538
domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, immunocytochemistry
quantity: 100 uL
price: US 464.00
to the supplier
domestic rabbit recombinant (27F2)
reactivity: human, rat
application: western blot, immunohistochemistry, immunocytochemistry, flow cytometry, immunohistochemistry - paraffin section
quantity: 100 uL
price: US 530.00
to the supplier
domestic rabbit recombinant (SA31-01)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunocytochemistry, flow cytometry, immunohistochemistry - paraffin section
quantity: 100 uL
price: US 470.00
to the supplier
domestic rabbit recombinant (JJ090-07)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section
quantity: 100 uL
price: US 470.00
to the supplier
domestic rabbit recombinant (JJ092-08)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section
quantity: 100 uL
price: US 470.00
to the supplier
Histone H3 Monoclonal Antibody (A11-D7)
Invitrogen
catalog: MA5-44628
mouse monoclonal (A11-D7)
reactivity: human, mouse, rat, zebrafish
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section
quantity: 100 uL
price: US 470.00
to the supplier
Histone H3 Monoclonal Antibody (6-A7)
Invitrogen
catalog: MA5-44629
mouse monoclonal (6-A7)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section
quantity: 100 uL
price: US 470.00
to the supplier
Histone H3 Monoclonal Antibody (3-C4)
Invitrogen
catalog: MA5-45047
mouse monoclonal (3-C4)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section
quantity: 100 uL
price: US 470.00
to the supplier
H3K27me1 Polyclonal Antibody
Invitrogen
catalog: PA5-116965
domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, ELISA, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section, dot blot
quantity: 100 uL
price: US 474.00
to the supplier
domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, ELISA, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section
quantity: 100 uL
price: US 474.00
to the supplier
domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, ELISA, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section
quantity: 100 uL
price: US 474.00
to the supplier
H3R12me2a Polyclonal Antibody
Invitrogen
catalog: PA5-120778
domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, ELISA
quantity: 100 uL
price: US 460.00
to the supplier
Histone H3 Polyclonal Antibody
Invitrogen
catalog: PA5-120808
domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, ELISA, immunohistochemistry, immunoprecipitation, immunohistochemistry - paraffin section
quantity: 100 uL
price: US 460.00
to the supplier
domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, ELISA
quantity: 100 uL
price: US 460.00
to the supplier
mouse recombinant (6-A7-R)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section
quantity: 100 uL
price: US 479.00
to the supplier
domestic rabbit recombinant (JE00-98)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section, dot blot
quantity: 100 uL
price: US 479.00
to the supplier