rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry, immunohistochemistry - frozen section
citations: 18
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry, immunohistochemistry - frozen section
citations: 18
Immunofluorescent analysis of Cyclophilin D in A431 Cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with a Cyclophilin D polyclonal antibody (Product # PA3-022) at a dilution of 1:200 overnight at 4 C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody (Product # 35503). Cyclophilin D staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Images were taken at 60X magnification.
Immunohistochemistry was performed on normal deparaffinized human Skeletalmuscle tissue. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:100 with a rabbit polyclonal antibody recognizing Cyclophilin D (Product #PA3-022) or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.
quantity: 100µL
price: US 390
to the supplier
rabbit polyclonal
reactivity: Chinese hamster, human, mouse, rat, dog
application: western blot
citations: 12
reactivity: Chinese hamster, human, mouse, rat, dog
application: western blot
citations: 12
rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, immunocytochemistry
citations: 2
reactivity: human, mouse, rat
application: western blot, immunocytochemistry
citations: 2
rabbit polyclonal
reactivity: human, mouse
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section
reactivity: human, mouse
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section
Immunofluorescence analysis of methanol-fixed HepG2 cells using Cyclophilin 40 (PA5-27988) antibody at 1:200 dilution.
Western Blot analysis of Cyclophilin 40 using anti-Cyclophilin 40 Polyclonal Antibody (PA5-27988), in Hep G2 Cell Lysate (30 ug of cell lysate) and at a dilution of 1:1000.
Western Blot analysis of Cyclophilin 40 using anti-Cyclophilin 40 Polyclonal Antibody (PA5-27988), in NIH-3T3 Cell Lysate (30 ug of cell lysate) and at a dilution of 1:1000.
quantity: 100µL
price: US 345
to the supplier