SLC2A5 Monoclonal Antibody (14C8)
Invitrogen
catalog: MA1-036
mouse monoclonal (14C8)
reactivity: human, mouse
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section
citations: 2

Immunohistochemistry was performed on cancer biopsies of deparaffinized human testis and hepatic carcinoma tissues. To expose target proteins heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer for 20 minutes at 95°C. Following antigen retrieval tissues were blocked in 10% normal goat serum (Product #31873) for 20 minutes at room temperature. Tissues were then probed at a dilution of 1:800 with a mouse monoclonal antibody recognizing SLC2A5 (Product # MA1-036) overnight at 4°C in a humidified chamber. Detection was performed using a goat anti-mouse HRP secondary antibody followed by colorimetric detection using DAB substrate. Tissues were counterstained with hematoxylin and prepped for mounting. Images were taken at 400X magnification. Results demonstrate membrane/cytoplasmic localization of SLC2A5.

Western blot analysis of SLC2A5 was performed by loading 25 ug of various whole cell lysates onto a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked with 5% Milk/TBST for at least 1 hour. Membranes were probed with a mouse monoclonal antibody recognizing SLC2A5 (Product # MA1-036) at a dilution of 1:1000 overnight at 4°C on a rocking platform. Membranes were washed in TBS-0.1% Tween 20 and probed with a goat anti-mouse-HRP secondary antibody (Product # 31430) at a dilution of 1:20,000 for at least one hour. Membranes were washed and chemiluminescent detection performed using Super Signal West Dura (Product #34075).

Immunofluorescent analysis of SLC2A5 using anti-SLC2A5 monoclonal antibody (Product# MA1-036) (shown in green) in HeLa cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature. Cells were then blocked with 1% Blocker BSA (Product #37525) for 15 minutes at room temperature. Cells were probed with a mouse monoclonal antibody recognizing SLC2A5 (Product# MA1-036), at a dilution of 1:100 for at least 1 hour at room temperature. Cells were then washed with PBS and incubated with DyLight 488 goat-anti-mouse secondary antibody (Product# 35503) at a dilution of 1:400 for 30 minutes at room temperature. Nuclei (blue) were stained with Hoechst 33342 dye (Product # 62249). Images were taken on a Thermo Scientific ArrayScan at 20X magnification.
quantity: 100 ug
price: US 491.00
to the supplier
SLC2A5 Monoclonal Antibody (14C8)
Invitrogen
catalog: MA1-036X
mouse monoclonal (14C8)
reactivity: human, mouse
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section
citations: 2

Immunofluorescent analysis of SLC2A5 using anti-SLC2A5 monoclonal antibody (Product# MA1-036) (shown in green) in HeLa cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature. Cells were then blocked with 1% Blocker BSA (Product #37525) for 15 minutes at room temperature. Cells were probed with a mouse monoclonal antibody recognizing SLC2A5 (Product# MA1-036), at a dilution of 1:100 for at least 1 hour at room temperature. Cells were then washed with PBS and incubated with DyLight 488 goat-anti-mouse secondary antibody (Product# 35503) at a dilution of 1:400 for 30 minutes at room temperature. Nuclei (blue) were stained with Hoechst 33342 dye (Product# 62249). Images were taken on a Thermo Scientific ArrayScan at 20X magnification.

Immunohistochemistry was performed on cancer biopsies of deparaffinized human testis and hepatic carcinoma tissues. To expose target proteins heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer for 20 minutes at 95°C. Following antigen retrieval tissues were blocked in 10% normal goat serum (Product #31873) for 20 minutes at room temperature. Tissues were then probed at a dilution of 1:800 with a mouse monoclonal antibody recognizing SLC2A5 (Product # MA1-036) overnight at 4°C in a humidified chamber. Detection was performed using a goat anti-mouse HRP secondary antibody followed by colorimetric detection using DAB substrate. Tissues were counterstained with hematoxylin and prepped for mounting. Images were taken at 400X magnification. Results demonstrate membrane/cytoplasmic localization of SLC2A5.

Western blot analysis of SLC2A5 was performed by loading 25ug of various whole cell lysates onto a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked with 5% Milk/TBST for at least 1 hour. Membranes were probed with a mouse monoclonal antibody recognizing SLC2A5 (Product # MA1-036) at a dilution of 1:1000 overnight at 4C on a rocking platform. Membranes were washed in TBS-0.1% Tween 20 and probed with a goat anti-mouse-HRP secondary antibody (Product # 31430) at a dilution of 1:20,000 for at least one hour. Membranes were washed and chemiluminescent detection performed using Super Signal West Dura (Product #34075).
quantity: 20 uL
price: US 187.00
to the supplier
GLUT5 Polyclonal Antibody
Invitrogen
catalog: PA5-80023
domestic rabbit polyclonal
reactivity: human, rat
application: western blot, immunohistochemistry, immunocytochemistry, flow cytometry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section
citations: 2
quantity: 100 ug
price: US 404.00
to the supplier
SLC2A5 Polyclonal Antibody
Invitrogen
catalog: PA5-42100
domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, immunocytochemistry
citations: 1
quantity: 100 uL
price: US 464.00
to the supplier
SLC2A5 Polyclonal Antibody
Invitrogen
catalog: PA5-82190
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section
quantity: 100 uL
price: US 522.00
to the supplier
SLC2A5 Polyclonal Antibody
Invitrogen
catalog: PA5-95120
domestic rabbit polyclonal
reactivity: human
application: western blot
quantity: 100 ug
price: US 464.00
to the supplier
GLUT5 Polyclonal Antibody
Invitrogen
catalog: PA5-89347
domestic rabbit polyclonal
reactivity: human, mouse
application: western blot, ELISA
quantity: 100 uL
price: US 464.00
to the supplier
GLUT5 Polyclonal Antibody
Invitrogen
catalog: PA5-115293
domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section
quantity: 100 uL
price: US 464.00
to the supplier
GLUT5 Polyclonal Antibody
Invitrogen
catalog: PA5-142485
domestic goat polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section
quantity: 100 ug
price: US 444.00
to the supplier