Calreticulin Polyclonal Antibody
Invitrogen
catalog: PA3-900

domestic rabbit polyclonal
reactivity: African green monkey, human, mouse, rat, dogs, domestic rabbit
application: western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry
citations: 74

Immunofluorescent analysis of Phalloidin (orange) and Calreticulin (green) in A431 cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in PBS for 10 minutes at room temperature and blocked with 2% BSA (Product # 37525) in PBS + 0.1% Triton X-100 for 30 minutes at room temperature. Cells were probed with a Calreticulin polyclonal antibody (Product # PA3-900) at a dilution of 1:75 for at least 1 hour at room temperature, washed with PBS, and incubated with DyLight 488 goat anti-rabbit IgG secondary antibody (Product # 35552) at a dilution of 1:250 for 30 minutes at room temperature. Actin was stained with DyLight 550 Phalloidin (Product # 21835) at a dilution of 1:120 (2.5 units/ml final concentration) and nuclei (blue) were stained with Hoechst (Product # 62249) at a concentration of 1ug/ml for 30 minutes. Images were taken on a Zeiss Axio Observer Z1 microscope at 20X magnification.

Immunofluorescent analysis of Calreticulin (green) U2OS cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in PBS for 10 minutes at room temperature and blocked with 2% BSA in PBS 0.1% triton-X (Product #37525) for 30 minutes at room temperature. Cells were probed with a rabbit polyclonal antibody recognizing Calreticulin (Product # PA3-900) at a dilution of 1:50 for at least 1 hour at room temperature. Cells were washed with PBS and incubated with DyLight 488 goat-anti-rabbit IgG secondary antibody (Product #35552) at a dilution of 1:250 for 30 minutes at room temperature. Actin filaments (red) were stained with DyLight 554-Phalloidin (Product# 21834) at a dilution of 1:300 (i.e. 1unit/mL final concentration) in PBS and incubated for 30 min including. Nuclei (blue) were stained with Hoechst 33342 dye (Product# 62249) at a dilution of 1µg/mL. Images were taken on a Thermo Scientific ArrayScan VTI at 20X magnification.

Immunofluorescent analysis of Phalloidin (green) and Calreticulin (red) in U2OS cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in PBS for 10 minutes at room temperature and blocked with 2% BSA (Product # 37525) in PBS + 0.1% Triton X-100 for 30 minutes at room temperature. Cells were probed with a Calreticulin polyclonal antibody (Product # PA3-900) at a dilution of 1:75 for at least 1 hour at room temperature, washed with PBS, and incubated with DyLight 633 goat anti-rabbit IgG secondary antibody (Product # 35562) at a dilution of 1:250 for 30 minutes at room temperature. Actin was stained with DyLight 488 Phalloidin (Product # 21833) at a dilution of 1:300 (1 unit/ml final concentration) and nuclei (blue) were stained with Hoechst (Product # 62249) at a concentration of 1ug/ml for 30 minutes. Images were taken on a Thermo Scientific ArrayScan VTI at 20X magnification.
quantity: 100 uL
price: US 496.00
to the supplier

Calreticulin Polyclonal Antibody
Invitrogen
catalog: PA1-902A

chicken polyclonal
reactivity: Chinese hamsters, human, mouse, rat, dogs
application: western blot, immunocytochemistry
citations: 7

quantity: 100 uL
price: US 490.00
to the supplier

Calreticulin Polyclonal Antibody
Invitrogen
catalog: PA1-903

chicken polyclonal
reactivity: Chinese hamsters, human, mouse, rat, dogs, domestic rabbit
application: western blot, immunocytochemistry, immunoprecipitation
citations: 3

quantity: 100 uL
price: US 471.00
to the supplier

Calreticulin Polyclonal Antibody
Invitrogen
catalog: PA5-25922

domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunocytochemistry, flow cytometry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section
citations: 2

quantity: 400 uL
price: US 538.00
to the supplier

Calreticulin Recombinant Rabbit Monoclonal Antibody (SU37-03)
Invitrogen
catalog: MA5-32131

domestic rabbit recombinant (SU37-03)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, flow cytometry, immunohistochemistry - paraffin section
citations: 1

quantity: 100 uL
price: US 483.00
to the supplier

Calreticulin Polyclonal Antibody
Invitrogen
catalog: PA3-16862

domestic rabbit polyclonal
reactivity: Chinese hamsters, human, mouse, rat, bovine
application: western blot, immunohistochemistry, immunocytochemistry, neutralization, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section, dot blot

Western blot on human kidney lysate.

Immunofluorescence staining of Calreticulin in HCT15 colon cancer cells using PA3-16862. Secondary antibody was an Alexa Fluor 488.
quantity: 100 uL
price: US 538.00
to the supplier

Calreticulin Polyclonal Antibody
Invitrogen
catalog: PA5-34786

domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunohistochemistry - paraffin section

quantity: 100 uL
price: US 558.00
to the supplier

Calreticulin Monoclonal Antibody (6C6)
Invitrogen
catalog: MA5-47360

mouse monoclonal (6C6)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunocytochemistry

quantity: 500 uL
price: US 1648.00
to the supplier

Calreticulin Monoclonal Antibody (6C6)
Invitrogen
catalog: MA5-47459

mouse monoclonal (6C6)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunocytochemistry

quantity: 100 uL
price: US 480.00
to the supplier

Calreticulin Recombinant Rabbit Monoclonal Antibody (10J1K8)
Invitrogen
catalog: MA5-51367

domestic rabbit recombinant (10J1K8)
reactivity: human, mouse, rat
application: western blot, ELISA, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

quantity: 100 uL
price: US 424.00
to the supplier

Calreticulin Polyclonal Antibody
Invitrogen
catalog: PA5-143146

domestic goat polyclonal
reactivity: human, rat
application: western blot

quantity: 100 ug
price: US 444.00
to the supplier

Calreticulin Recombinant Rabbit Monoclonal Antibody (23GB700)
Invitrogen
catalog: MA5-53202

domestic rabbit recombinant (23GB700)
reactivity: human, mouse, rat
application: western blot, immunocytochemistry, flow cytometry

quantity: 100 uL
price: US 539.00
to the supplier

Calreticulin Recombinant Rabbit Monoclonal Antibody (23GB700)
Invitrogen
catalog: MA5-53203

domestic rabbit recombinant (23GB700)
reactivity: human, mouse, rat
application: western blot, immunocytochemistry, flow cytometry

quantity: 20 uL
price: US 199.00
to the supplier

Calreticulin Recombinant Rabbit Monoclonal Antibody (7T5B2)
Invitrogen
catalog: MA5-58021

domestic rabbit recombinant (7T5B2)
reactivity: human, mouse, rat
application: western blot, ELISA

quantity: 20 uL
price: US 169.00
to the supplier

Calreticulin Recombinant Rabbit Monoclonal Antibody (7T5B2)
Invitrogen
catalog: MA5-58022

domestic rabbit recombinant (7T5B2)
reactivity: human, mouse, rat
application: western blot, ELISA

quantity: 100 uL
price: US 419.00
to the supplier