alpha Tubulin Monoclonal Antibody (DM1A) | Invitrogen 62204 product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

alpha Tubulin Monoclonal Antibody (DM1A)

catalog :

62204

quantity :

40 µL

price :

US 225.00

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

DM1A

reactivity :

hamsters, Leishmania infantum, guinea pig, African green monkey, freshwater planarian, human, mouse, rat, dogs, chicken, bovine, pigs , fruit fly , domestic sheep, Xenopus laevis

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunohistochemistry - paraffin section, other

more info or order :

Invitrogen product webpage

citations: 134

Published Application/Species/Sample/Dilution	Reference
other; freshwater planarian; 1:1000	Karge A, Bonar N, Wood S, Petersen C. tec-1 kinase negatively regulates regenerative neurogenesis in planarians. elife. 2020;9: pubmed publisher
western blot; mouse; 1:1000; fig 2s1a	Clark D, McMillan L, Tan S, Bellomo G, Massoue C, Thompson H, et al. Transient protein accumulation at the center of the T cell antigen-presenting cell interface drives efficient IL-2 secretion. elife. 2019;8: pubmed publisher
western blot; human; loading ...; fig 3f	Lai Y, Zhu M, Wu W, Rokutanda N, Togashi Y, Liang W, et al. HERC2 regulates RPA2 by mediating ATR-induced Ser33 phosphorylation and ubiquitin-dependent degradation. Sci Rep. 2019;9:14257 pubmed publisher
immunohistochemistry - paraffin section; mouse; loading ...	Jung J, Jung H, Neupane S, Kim K, Kim J, Yamamoto H, et al. Involvement of PI3K and PKA pathways in mouse tongue epithelial differentiation. Acta Histochem. 2017;119:92-98 pubmed publisher
immunohistochemistry; mouse; loading ...; fig 5f	Da Ros M, Lehtiniemi T, Olotu O, Fischer D, Zhang F, Vihinen H, et al. FYCO1 and autophagy control the integrity of the haploid male germ cell-specific RNP granules. Autophagy. 2017;13:302-321 pubmed publisher
western blot; human; loading ...; fig 2C	Vuono E, Mukherjee A, Vierra D, Adroved M, Hodson C, Deans A, et al. The PTEN phosphatase functions cooperatively with the Fanconi anemia proteins in DNA crosslink repair. Sci Rep. 2016;6:36439 pubmed publisher
western blot; rat; 1:2000; loading ...; fig 3c	Oksdath M, Guil A, Grassi D, Sosa L, Quiroga S. The Motor KIF5C Links the Requirements of Stable Microtubules and IGF-1 Receptor Membrane Insertion for Neuronal Polarization. Mol Neurobiol. 2017;54:6085-6096 pubmed publisher
western blot; human; fig 1	Naidenow J, Hrgovic I, Doll M, Hailemariam Jahn T, Lang V, Kleemann J, et al. Peroxisome proliferator-activated receptor (PPAR) ? and ? activators induce ICAM-1 expression in quiescent non stimulated endothelial cells. J Inflamm (Lond). 2016;13:27 pubmed publisher
immunocytochemistry; human; fig 1 western blot; human; fig 1	Peng H, Cheng Y, Hsu Y, Wu G, Kuo C, Liou J, et al. MPT0B098, a Microtubule Inhibitor, Suppresses JAK2/STAT3 Signaling Pathway through Modulation of SOCS3 Stability in Oral Squamous Cell Carcinoma. PLoS ONE. 2016;11:e0158440 pubmed publisher
western blot; mouse; fig 3	Hamlin A, Basford J, Jaeschke A, Hui D. LRP1 Protein Deficiency Exacerbates Palmitate-induced Steatosis and Toxicity in Hepatocytes. J Biol Chem. 2016;291:16610-9 pubmed publisher
western blot; human; fig 3	Du Z, Li L, Huang X, Jin J, Huang S, Zhang Q, et al. The epigenetic modifier CHD5 functions as a novel tumor suppressor for renal cell carcinoma and is predominantly inactivated by promoter CpG methylation. Oncotarget. 2016;7:21618-30 pubmed publisher
western blot; human; fig 4b	Chuang T, Lee K, Lou Y, Lu C, Tarn W. A Point Mutation in the Exon Junction Complex Factor Y14 Disrupts Its Function in mRNA Cap Binding and Translation Enhancement. J Biol Chem. 2016;291:8565-74 pubmed publisher
western blot; human; 1:2500; fig 5	Maiden S, Petrova Y, Gumbiner B. Microtubules Inhibit E-Cadherin Adhesive Activity by Maintaining Phosphorylated p120-Catenin in a Colon Carcinoma Cell Model. PLoS ONE. 2016;11:e0148574 pubmed publisher
western blot; human; fig 2	Ikeda S, Kitadate A, Ito M, Abe F, Nara M, Watanabe A, et al. Disruption of CCL20-CCR6 interaction inhibits metastasis of advanced cutaneous T-cell lymphoma. Oncotarget. 2016;7:13563-74 pubmed publisher
western blot; Leishmania infantum; 1:1000; fig 4	Faria J, Loureiro I, Santarém N, Macedo Ribeiro S, Tavares J, Cordeiro da Silva A. Leishmania infantum Asparagine Synthetase A Is Dispensable for Parasites Survival and Infectivity. PLoS Negl Trop Dis. 2016;10:e0004365 pubmed publisher
western blot; human; fig 7	Yen Y, Hsiao J, Jiang S, Chang J, Wang S, Shen Y, et al. Insulin-like growth factor-independent insulin-like growth factor binding protein 3 promotes cell migration and lymph node metastasis of oral squamous cell carcinoma cells by requirement of integrin β1. Oncotarget. 2015;6:41837-55 pubmed publisher
western blot; human; fig 3	Roos A, Satterfield L, Zhao S, Fuja D, Shuck R, Hicks M, et al. Loss of Runx2 sensitises osteosarcoma to chemotherapy-induced apoptosis. Br J Cancer. 2015;113:1289-97 pubmed publisher
immunocytochemistry; mouse; fig 3 western blot; mouse; fig 2	Panet E, Ozer E, Mashriki T, Lazar I, Itzkovich D, Tzur A. Purifying Cytokinetic Cells from an Asynchronous Population. Sci Rep. 2015;5:13230 pubmed publisher
immunohistochemistry; mouse; 1:1000	Lehti M, Kotaja N, Sironen A. KIF1-binding protein interacts with KIF3A in haploid male germ cells. Reproduction. 2015;150:209-16 pubmed publisher
western blot; human; fig 2	Bach A, Derocq D, Laurent Matha V, Montcourrier P, Sebti S, Orsetti B, et al. Nuclear cathepsin D enhances TRPS1 transcriptional repressor function to regulate cell cycle progression and transformation in human breast cancer cells. Oncotarget. 2015;6:28084-103 pubmed publisher
western blot; human; 1:2000	Kiss A, Gong X, Kowalewski J, Shafqat Abbasi H, StrÃ¶mblad S, Lock J. Non-monotonic cellular responses to heterogeneity in talin protein expression-level. Integr Biol (Camb). 2015;7:1171-85 pubmed publisher
western blot; human	Teng Y, Radde B, Litchfield L, Ivanova M, Prough R, Clark B, et al. Dehydroepiandrosterone Activation of G-protein-coupled Estrogen Receptor Rapidly Stimulates MicroRNA-21 Transcription in Human Hepatocellular Carcinoma Cells. J Biol Chem. 2015;290:15799-811 pubmed publisher
western blot; human; fig 1	Ma S, Jiang B, Deng W, Gu Z, Wu F, Li T, et al. D-2-hydroxyglutarate is essential for maintaining oncogenic property of mutant IDH-containing cancer cells but dispensable for cell growth. Oncotarget. 2015;6:8606-20 pubmed
western blot; human; 1:5000; fig 2	Nakagawa Y, Sedukhina A, Okamoto N, Nagasawa S, Suzuki N, Ohta T, et al. NF-ÎºB signaling mediates acquired resistance after PARP inhibition. Oncotarget. 2015;6:3825-39 pubmed
western blot; mouse; fig 1	West A, Khoury Hanold W, Staron M, Tal M, Pineda C, Lang S, et al. Mitochondrial DNA stress primes the antiviral innate immune response. Nature. 2015;520:553-7 pubmed publisher
western blot; mouse; fig 2	Chuang C, Guh J, Lu C, Chen H, Chuang L. S100B is required for high glucose-induced pro-fibrotic gene expression and hypertrophy in mesangial cells. Int J Mol Med. 2015;35:546-52 pubmed publisher
western blot; human; fig 9	Colbert P, Vermeer D, Wieking B, Lee J, Vermeer P. EphrinB1: novel microtubule associated protein whose expression affects taxane sensitivity. Oncotarget. 2015;6:953-68 pubmed
western blot; human; fig 3a	Lee K, Tarn W. TRAP150 activates splicing in composite terminal exons. Nucleic Acids Res. 2014;42:12822-32 pubmed publisher
western blot; human; fig 3	Gray A, Stephens C, Bigelow R, Coleman D, Cardelli J. The polyphenols (-)-epigallocatechin-3-gallate and luteolin synergistically inhibit TGF-Î²-induced myofibroblast phenotypes through RhoA and ERK inhibition. PLoS ONE. 2014;9:e109208 pubmed publisher
western blot; Xenopus laevis; fig 2	Curran K, Allen L, Porter B, Dodge J, Lope C, Willadsen G, et al. Circadian genes, xBmal1 and xNocturnin, modulate the timing and differentiation of somites in Xenopus laevis. PLoS ONE. 2014;9:e108266 pubmed publisher
western blot; human; fig 5	Brohl A, Solomon D, Chang W, Wang J, Song Y, Sindiri S, et al. The genomic landscape of the Ewing Sarcoma family of tumors reveals recurrent STAG2 mutation. PLoS Genet. 2014;10:e1004475 pubmed publisher
western blot; human; fig 3	Elzi D, Song M, Hakala K, Weintraub S, Shiio Y. Proteomic Analysis of the EWS-Fli-1 Interactome Reveals the Role of the Lysosome in EWS-Fli-1 Turnover. J Proteome Res. 2014;13:3783-91 pubmed publisher
western blot; human; 1:1000	Yen T, Lu W, Lien L, Thomas P, Lee T, Chiu H, et al. Amarogentin, a secoiridoid glycoside, abrogates platelet activation through PLC ? 2-PKC and MAPK pathways. Biomed Res Int. 2014;2014:728019 pubmed publisher
western blot; human	Teng Y, Litchfield L, Ivanova M, Prough R, Clark B, Klinge C. Dehydroepiandrosterone-induces miR-21 transcription in HepG2 cells through estrogen receptor ? and androgen receptor. Mol Cell Endocrinol. 2014;392:23-36 pubmed publisher
western blot; human	Skvortsov S, Dudas J, Eichberger P, Witsch Baumgartner M, Loeffler Ragg J, Pritz C, et al. Rac1 as a potential therapeutic target for chemo-radioresistant head and neck squamous cell carcinomas (HNSCC). Br J Cancer. 2014;110:2677-87 pubmed publisher
immunohistochemistry; mouse; 1:1000 western blot; mouse; 1:500	Aguilar A, Becker L, Tedeschi T, Heller S, Iomini C, Nachury M. Α-tubulin K40 acetylation is required for contact inhibition of proliferation and cell-substrate adhesion. Mol Biol Cell. 2014;25:1854-66 pubmed publisher
western blot; mouse	Kondo S, Bottos A, Allegood J, Masson R, Maurer F, Genoud C, et al. Memo has a novel role in S1P signaling and is [corrected] crucial for vascular development. PLoS ONE. 2014;9:e94114 pubmed publisher
western blot; mouse	Yamamoto R, Ohshiro Y, Shimotani T, Yamamoto M, Matsuyama S, Ide H, et al. Hypersensitivity of mouse NEIL1-knockdown cells to hydrogen peroxide during S phase. J Radiat Res. 2014;55:707-12 pubmed publisher
western blot; mouse	Kuwahara M, Suzuki J, Tofukuji S, Yamada T, Kanoh M, Matsumoto A, et al. The Menin-Bach2 axis is critical for regulating CD4 T-cell senescence and cytokine homeostasis. Nat Commun. 2014;5:3555 pubmed publisher
western blot; domestic sheep; 1:1000	Patel A, Tripathi A, Patel U, Shah R, Joshi C. Myostatin knockdown and its effect on myogenic gene expression program in stably transfected goat myoblasts. In Vitro Cell Dev Biol Anim. 2014;50:587-96 pubmed publisher
western blot; human	Konrath F, Witt J, Sauter T, Kulms D. Identification of new I?B? complexes by an iterative experimental and mathematical modeling approach. PLoS Comput Biol. 2014;10:e1003528 pubmed publisher
western blot; human	Lin K, Lu W, Wang S, Fong T, Chou D, Chang C, et al. Characteristics of endogenous ?-aminobutyric acid (GABA) in human platelets: functional studies of a novel collagen glycoprotein VI inhibitor. J Mol Med (Berl). 2014;92:603-14 pubmed publisher
western blot; mouse; 1:5000	Sanz Moreno A, Fuhrmann D, Wolf E, von Eyss B, Eilers M, Elsässer H. Miz1 deficiency in the mammary gland causes a lactation defect by attenuated Stat5 expression and phosphorylation. PLoS ONE. 2014;9:e89187 pubmed publisher
western blot; rat	Gao Y, Ji B. N1-(Quinolin-2-ylmethyl) butane-1, 4-diamine, a polyamine analog: inhibition of platelet aggregation mediated by an elevated VASP, and decreased MAPK phosphorylation. Blood Coagul Fibrinolysis. 2014;25:476-85 pubmed publisher
western blot; human	Lu W, Wu M, Lin K, Lin Y, Chou H, Sheu J. Hinokitiol is a novel glycoprotein VI antagonist on human platelets. Platelets. 2014;25:595-602 pubmed publisher
western blot; human	Laketa V, Zarbakhsh S, TRAYNOR KAPLAN A, MacNamara A, Subramanian D, Putyrski M, et al. PIP? induces the recycling of receptor tyrosine kinases. Sci Signal. 2014;7:ra5 pubmed publisher
immunocytochemistry; human western blot; human	Yen Y, Shiah S, Chu H, Hsu Y, Hsiao J, Chang J, et al. Reciprocal regulation of microRNA-99a and insulin-like growth factor I receptor signaling in oral squamous cell carcinoma cells. Mol Cancer. 2014;13:6 pubmed publisher
immunohistochemistry; human; 1:200	Kunishima S, Nishimura S, Suzuki H, Imaizumi M, Saito H. TUBB1 mutation disrupting microtubule assembly impairs proplatelet formation and results in congenital macrothrombocytopenia. Eur J Haematol. 2014;92:276-82 pubmed publisher
western blot; human	Arlt M, Rajendran S, Birkeland S, Wilson T, Glover T. Copy number variants are produced in response to low-dose ionizing radiation in cultured cells. Environ Mol Mutagen. 2014;55:103-13 pubmed publisher
western blot; mouse; fig 1	Basford J, Koch S, Anjak A, Singh V, Krause E, Robbins N, et al. Smooth muscle LDL receptor-related protein-1 deletion induces aortic insufficiency and promotes vascular cardiomyopathy in mice. PLoS ONE. 2013;8:e82026 pubmed publisher
western blot; human	Boisvert R, Rego M, Azzinaro P, Mauro M, Howlett N. Coordinate nuclear targeting of the FANCD2 and FANCI proteins via a FANCD2 nuclear localization signal. PLoS ONE. 2013;8:e81387 pubmed publisher
western blot; human	Tomizawa M, Shinozaki F, Motoyoshi Y, Sugiyama T, Yamamoto S, Sueishi M, et al. Niclosamide suppresses Hepatoma cell proliferation via the Wnt pathway. Onco Targets Ther. 2013;6:1685-93 pubmed publisher
western blot; human	El Mesery M, Trebing J, Schafer V, Weisenberger D, Siegmund D, Wajant H. CD40-directed scFv-TRAIL fusion proteins induce CD40-restricted tumor cell death and activate dendritic cells. Cell Death Dis. 2013;4:e916 pubmed publisher
western blot; human; 1:5000	Chang K, Chang W, Chang Y, Hung L, Lai C, Yeh Y, et al. Ran GTPase-activating protein 1 is a therapeutic target in diffuse large B-cell lymphoma. PLoS ONE. 2013;8:e79863 pubmed publisher
immunohistochemistry; Xenopus laevis; 1:500 western blot; Xenopus laevis; 1:10000	Wang S, Cha S, Zorn A, Wylie C. Par6b regulates the dynamics of apicobasal polarity during development of the stratified Xenopus epidermis. PLoS ONE. 2013;8:e76854 pubmed publisher
western blot; mouse; fig 3	Chen Y, Pan H, Tseng H, Chu H, Hung Y, Yen Y, et al. Differentiated epithelial- and mesenchymal-like phenotypes in subcutaneous mouse xenografts using diffusion weighted-magnetic resonance imaging. Int J Mol Sci. 2013;14:21943-59 pubmed publisher
western blot; human	Solomon D, Kim J, Bondaruk J, Shariat S, Wang Z, Elkahloun A, et al. Frequent truncating mutations of STAG2 in bladder cancer. Nat Genet. 2013;45:1428-30 pubmed publisher
immunohistochemistry; human	Chanthammachat P, Promwikorn W, Pruegsanusak K, Roytrakul S, Srisomsap C, Chokchaichamnankit D, et al. Comparative proteomic analysis of oral squamous cell carcinoma and adjacent non-tumour tissue from Thailand. Arch Oral Biol. 2013;58:1677-85 pubmed publisher
western blot; mouse	Simonatto M, Marullo F, Chiacchiera F, Musaro A, Wang J, Latella L, et al. DNA damage-activated ABL-MyoD signaling contributes to DNA repair in skeletal myoblasts. Cell Death Differ. 2013;20:1664-74 pubmed publisher
immunocytochemistry; fruit fly	Zhang D, Asenjo A, Greenbaum M, Xie L, Sharp D, Sosa H. A second tubulin binding site on the kinesin-13 motor head domain is important during mitosis. PLoS ONE. 2013;8:e73075 pubmed publisher
western blot; human	Richter F, Liebig T, Guenzi E, Herrmann A, Scheurich P, Pfizenmaier K, et al. Antagonistic TNF receptor one-specific antibody (ATROSAB): receptor binding and in vitro bioactivity. PLoS ONE. 2013;8:e72156 pubmed publisher
western blot; human	Cohen M, Vecsler M, Liberzon A, Noach M, Zlotorynski E, Tzur A. Unbiased transcriptome signature of in vivo cell proliferation reveals pro- and antiproliferative gene networks. Cell Cycle. 2013;12:2992-3000 pubmed publisher
western blot; mouse; 1:2000	Oderup C, Lajevic M, Butcher E. Canonical and noncanonical Wnt proteins program dendritic cell responses for tolerance. J Immunol. 2013;190:6126-34 pubmed publisher
western blot; human	Shu X, Li L, Ji M, Cheng Y, Ying J, Fan Y, et al. FEZF2, a novel 3p14 tumor suppressor gene, represses oncogene EZH2 and MDM2 expression and is frequently methylated in nasopharyngeal carcinoma. Carcinogenesis. 2013;34:1984-93 pubmed publisher
western blot; mouse	Suzuki J, Kuwahara M, Tofukuji S, Imamura M, Kato F, Nakayama T, et al. A novel small compound SH-2251 suppresses Th2 cell-dependent airway inflammation through selective modulation of chromatin status at the Il5 gene locus. PLoS ONE. 2013;8:e61785 pubmed publisher
western blot; human	Verdoodt B, Neid M, Vogt M, Kuhn V, Liffers S, Palisaar R, et al. MicroRNA-205, a novel regulator of the anti-apoptotic protein Bcl2, is downregulated in prostate cancer. Int J Oncol. 2013;43:307-14 pubmed publisher
immunocytochemistry; hamsters immunocytochemistry; mouse	Rodriguez Martin T, Cuchillo Ibanez I, Noble W, Nyenya F, Anderton B, Hanger D. Tau phosphorylation affects its axonal transport and degradation. Neurobiol Aging. 2013;34:2146-57 pubmed publisher
western blot; human	Chang Y, Chen W, Lin K, Hsieh C, Chou D, Lin L, et al. Novel bioactivity of ellagic Acid in inhibiting human platelet activation. Evid Based Complement Alternat Med. 2013;2013:595128 pubmed publisher
western blot; human	Kakinuma Y, Tsuda M, Okazaki K, Akiyama T, Arikawa M, Noguchi T, et al. Heart-specific overexpression of choline acetyltransferase gene protects murine heart against ischemia through hypoxia-inducible factor-1?-related defense mechanisms. J Am Heart Assoc. 2013;2:e004887 pubmed publisher
western blot; human	Lin K, Kuo J, Lu W, Chung C, Chou D, Huang S, et al. Hinokitiol inhibits platelet activation ex vivo and thrombus formation in vivo. Biochem Pharmacol. 2013;85:1478-85 pubmed publisher
western blot; human	Wang Y, Lin K, Chen S, Gu D, Chen C, Tu P, et al. Overexpressed-eIF3I interacted and activated oncogenic Akt1 is a theranostic target in human hepatocellular carcinoma. Hepatology. 2013;58:239-50 pubmed publisher
western blot; human	Lien L, Su C, Hsu W, Lu W, Chung C, Yen T, et al. Mechanisms of andrographolide-induced platelet apoptosis in human platelets: regulatory roles of the extrinsic apoptotic pathway. Phytother Res. 2013;27:1671-7 pubmed publisher
western blot; human	Kaluza D, Kroll J, Gesierich S, Manavski Y, Boeckel J, Doebele C, et al. Histone deacetylase 9 promotes angiogenesis by targeting the antiangiogenic microRNA-17-92 cluster in endothelial cells. Arterioscler Thromb Vasc Biol. 2013;33:533-43 pubmed publisher
western blot; human	Jayakumar T, Chen W, Lu W, Chou D, Hsiao G, Hsu C, et al. A novel antithrombotic effect of sulforaphane via activation of platelet adenylate cyclase: ex vivo and in vivo studies. J Nutr Biochem. 2013;24:1086-95 pubmed publisher
western blot; human	Fritz V, Benfodda Z, Henriquet C, Hure S, Cristol J, Michel F, et al. Metabolic intervention on lipid synthesis converging pathways abrogates prostate cancer growth. Oncogene. 2013;32:5101-10 pubmed publisher
western blot; mouse	Beez S, Demmer P, Puccetti E. Targeting the acute promyelocytic leukemia-associated fusion proteins PML/RAR? and PLZF/RAR? with interfering peptides. PLoS ONE. 2012;7:e48636 pubmed publisher
western blot; human; 0.3 ug/ml	Chuang T, Chang W, Lee K, Tarn W. The RNA-binding protein Y14 inhibits mRNA decapping and modulates processing body formation. Mol Biol Cell. 2013;24:1-13 pubmed publisher
immunocytochemistry; human; 1:500	Alushin G, Musinipally V, Matson D, Tooley J, Stukenberg P, Nogales E. Multimodal microtubule binding by the Ndc80 kinetochore complex. Nat Struct Mol Biol. 2012;19:1161-7 pubmed publisher
western blot; human	Queen K, Shi M, Zhang F, Cvek U, Scott R. Epstein-Barr virus-induced epigenetic alterations following transient infection. Int J Cancer. 2013;132:2076-86 pubmed publisher
western blot; domestic sheep; 0.01 ug/ml	Tan B, Blanco A, Houston E, Stewart P, Goldmann W, Gill A, et al. Significant differences in incubation times in sheep infected with bovine spongiform encephalopathy result from variation at codon 141 in the PRNP gene. J Gen Virol. 2012;93:2749-56 pubmed publisher
western blot; human	Lin Z, Chu H, Yen Y, Lewis B, Chen Y. Krüppel-like factor 4, a tumor suppressor in hepatocellular carcinoma cells reverts epithelial mesenchymal transition by suppressing slug expression. PLoS ONE. 2012;7:e43593 pubmed publisher
immunohistochemistry; Xenopus laevis; 1:500	Ohgami H, Hiyoshi M, Mostafa M, Kubo H, Abe S, Takamune K. Xtr, a plural tudor domain-containing protein, is involved in the translational regulation of maternal mRNA during oocyte maturation in Xenopus laevis. Dev Growth Differ. 2012;54:660-71 pubmed publisher
western blot; African green monkey	Rego M, Kolling F, Vuono E, Mauro M, Howlett N. Regulation of the Fanconi anemia pathway by a CUE ubiquitin-binding domain in the FANCD2 protein. Blood. 2012;120:2109-17 pubmed publisher
western blot; Xenopus laevis; 1:5000; fig 1e	Cha S, McAdams M, Kormish J, Wylie C, Kofron M. Foxi2 is an animally localized maternal mRNA in Xenopus, and an activator of the zygotic ectoderm activator Foxi1e. PLoS ONE. 2012;7:e41782 pubmed publisher
western blot; human	Witt J, Konrath F, Sawodny O, Ederer M, Kulms D, Sauter T. Analysing the role of UVB-induced translational inhibition and PP2Ac deactivation in NF-?B signalling using a minimal mathematical model. PLoS ONE. 2012;7:e40274 pubmed publisher
western blot; human	Lu W, Lin K, Hsu M, Chou D, Hsiao G, Sheu J. Suppression of NF-?B signaling by andrographolide with a novel mechanism in human platelets: regulatory roles of the p38 MAPK-hydroxyl radical-ERK2 cascade. Biochem Pharmacol. 2012;84:914-24 pubmed publisher
immunocytochemistry; human; 1:500	Simpson J, Joggerst B, Laketa V, Verissimo F, Cetin C, Erfle H, et al. Genome-wide RNAi screening identifies human proteins with a regulatory function in the early secretory pathway. Nat Cell Biol. 2012;14:764-74 pubmed publisher
western blot; human	Lee Y, Hsieh K, Lu W, Chou H, Chou D, Lien L, et al. Xanthohumol, a Prenylated Flavonoid from Hops (Humulus lupulus), Prevents Platelet Activation in Human Platelets. Evid Based Complement Alternat Med. 2012;2012:852362 pubmed publisher
western blot; human; 1:10000	Zimmermann M, Arachchige Don A, Donaldson M, Dallapiazza R, Cowan C, Horne M. Elevated cyclin G2 expression intersects with DNA damage checkpoint signaling and is required for a potent G2/M checkpoint arrest response to doxorubicin. J Biol Chem. 2012;287:22838-53 pubmed publisher
western blot; human	Hsueh S, Du J, Hsu W, Fang C, Liu H, Wang W. SV40 T/t-common polypeptide enhances the sensitivity of HER2-overexpressing human cancer cells to anticancer drugs cisplatin and doxorubicin. Cancer Lett. 2012;324:48-57 pubmed publisher
western blot; human	Tomizawa M, Shinozaki F, Sugiyama T, Yamamoto S, Sueishi M, Yoshida T. Insulin-like growth factor I receptor involvement in proliferation of NOR-P1 cells in serum-free media. J Cell Biochem. 2012;113:2714-20 pubmed publisher
western blot; human	Raimundo N, Song L, Shutt T, McKay S, Cotney J, Guan M, et al. Mitochondrial stress engages E2F1 apoptotic signaling to cause deafness. Cell. 2012;148:716-26 pubmed publisher
western blot; human	Townley A, Schmidt K, Hodgson L, Stephens D. Epithelial organization and cyst lumen expansion require efficient Sec13-Sec31-driven secretion. J Cell Sci. 2012;125:673-84 pubmed publisher
western blot; human	VALENCIA S, Hutt Fletcher L. Important but differential roles for actin in trafficking of Epstein-Barr virus in B cells and epithelial cells. J Virol. 2012;86:2-10 pubmed publisher
immunocytochemistry; mouse; 1:2000	Daghestani H, Zhu G, Johnston P, Shinde S, Brodsky J, Day B. Characterization of inhibitors of glucocorticoid receptor nuclear translocation: a model of cytoplasmic dynein-mediated cargo transport. Assay Drug Dev Technol. 2012;10:46-60 pubmed publisher
western blot; human; fig 7	Rauert H, Stühmer T, Bargou R, Wajant H, Siegmund D. TNFR1 and TNFR2 regulate the extrinsic apoptotic pathway in myeloma cells by multiple mechanisms. Cell Death Dis. 2011;2:e194 pubmed publisher
western blot; mouse	Carpenter T, Schroeder W, Stenmark K, Schmidt E. Eph-A2 promotes permeability and inflammatory responses to bleomycin-induced lung injury. Am J Respir Cell Mol Biol. 2012;46:40-7 pubmed publisher
immunocytochemistry; human; 1:2000; fig 1	Tooley J, Miller S, Stukenberg P. The Ndc80 complex uses a tripartite attachment point to couple microtubule depolymerization to chromosome movement. Mol Biol Cell. 2011;22:1217-26 pubmed publisher
western blot; mouse; fig 7 western blot; human; fig 7	Itahana K, Zhang Y. Mitochondrial p32 is a critical mediator of ARF-induced apoptosis. Cancer Cell. 2008;13:542-53 pubmed publisher
western blot; mouse; 1:2000	Lü L, Li J, Yew D, Rudd J, Mak Y. Oxidative stress on the astrocytes in culture derived from a senescence accelerated mouse strain. Neurochem Int. 2008;52:282-9 pubmed
western blot; human	De Fanis U, Mori F, Kurnat R, Lee W, Bova M, Adkinson N, et al. GATA3 up-regulation associated with surface expression of CD294/CRTH2: a unique feature of human Th cells. Blood. 2007;109:4343-50 pubmed
western blot; human; fig 2	Kim J, Lee C, Bonifant C, Ressom H, Waldman T. Activation of p53-dependent growth suppression in human cells by mutations in PTEN or PIK3CA. Mol Cell Biol. 2007;27:662-77 pubmed
western blot; human; 1:800; fig 3	Barlow J, Wiley J, Mous M, Narendran A, Gee M, Goldberg M, et al. Differentiation of rhabdomyosarcoma cell lines using retinoic acid. Pediatr Blood Cancer. 2006;47:773-84 pubmed
immunocytochemistry; human; 1:100	Yvon A, Wadsworth P, Jordan M. Taxol suppresses dynamics of individual microtubules in living human tumor cells. Mol Biol Cell. 1999;10:947-59 pubmed
	Chaigne A, Labouesse C, White I, Agnew M, Hannezo E, Chalut K, et al. Abscission Couples Cell Division to Embryonic Stem Cell Fate. Dev Cell. 2020;55:195-208.e5 pubmed publisher
	Federico A, Steinfass T, Larribere L, Novak D, Morís F, Núñez L, et al. Mithramycin A and Mithralog EC-8042 Inhibit SETDB1 Expression and Its Oncogenic Activity in Malignant Melanoma. Mol Ther Oncolytics. 2020;18:83-99 pubmed publisher
	Booth D, King N. Genome editing enables reverse genetics of multicellular development in the choanoflagellate Salpingoeca rosetta. elife. 2020;9: pubmed publisher
	Tisi A, Parete G, Flati V, Maccarone R. Up-regulation of pro-angiogenic pathways and induction of neovascularization by an acute retinal light damage. Sci Rep. 2020;10:6376 pubmed publisher
	Raeker M, Pierre Charles J, Carethers J. Tetranucleotide Microsatellite Mutational Behavior Assessed in Real Time: Implications for Future Microsatellite Panels. Cell Mol Gastroenterol Hepatol. 2020;9:689-704 pubmed publisher
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product information

Product Type :

Antibody

Product Name :

alpha Tubulin Monoclonal Antibody (DM1A)

Catalog # :

62204

Quantity :

40 µL

Price :

US 225.00

Clonality :

Monoclonal

Purity :

Protein G

Host :

Mouse

Reactivity :

Amphibian, Bovine, Canine, Chicken, Gerbil, Guinea Pig, Human, Mouse, Porcine, Rat

Applications :

Immunocytochemistry: Assay-dependent, Immunohistochemistry (Paraffin): 1:400, Immunomicroscopy: Assay-dependent, Immunoprecipitation: 2 µg/mg, Western Blot: 1 µg/mL

Species :

Amphibian, Bovine, Canine, Chicken, Gerbil, Guinea Pig, Human, Mouse, Porcine, Rat

Clone :

DM1A

Isotype :

IgG1, kappa

Storage :

4° C

Description :

Alpha Tubulin is a part of a heterodimer consisting of alpha and beta tubulin subunits. The alpha and beta tubulins, which are each about 55 kDa MW, are homologous but not identical. Alpha-beta tubulin heterodimer is the basic building block of microtubules, and this intracellular cylindrical filamentous structure is present in almost eukaryotic cells. Microtubules serve as structural supports and lines of transport within the cell, as well as serving a key role in mitosis. Microtubules of the eukaryotic cytoskeleton perform essential and diverse functions and are composed of a heterodimer of alpha and beta tubulins. The alpha and beta tubulins represent the major components of microtubules, while gamma tubulin plays a critical role in the nucleation of microtubule assembly. There are multiple alpha and beta tubulin genes, which are highly conserved among species. The gene that encodes alpha tubulin is highly similar to the mouse and rat Tuba1 gene. Northern blotting studies have shown that the gene expression is predominantly found in morphologically differentiated neurologic cells.

Immunogen :

Native chick brain microtubules

Format :

Liquid

Applications w/Dilutions :

Immunocytochemistry: Assay-dependent, Immunohistochemistry (Paraffin): 1:400, Immunomicroscopy: Assay-dependent, Immunoprecipitation: 2 µg/mg, Western Blot: 1 µg/mL

Aliases :

&agr-tubulin 84B; 1t; a1t; ALPHA 84C; alpha tubulin; alpha tubulin 84B; alpha[[1]] tubulin; alpha[[1]]-tubulin; alpha1; alpha1 tubulin; alpha1t; alpha1tub; alpha1tub84B; alpha1-tubulin; alpha84B; alphaT; alphat-1; alphatub; alpha-tub; alphaTub1; alphatub84; alphaTUB84B; alpha-tub84B; alphaTub84B-PA; alphatubulin; alpha-tubulin; Alpha-tubulin 1; Alpha-tubulin 2; Alpha-tubulin 3; Alpha-tubulin 3C; alpha-tubulin 3C/D; Alpha-tubulin 3D; Alpha-tubulin 3E; alpha-tubulin 4; Alpha-tubulin 6; alphaTubulin 84B; alpha-Tubulin 84B; alpha-Tubulin at 84B; alpha-tubulin I; Alpha-tubulin II; alpha-tubulin III; Alpha-tubulin isotype M-alpha-1; alpha-tubulin isotype M-alpha-2; Alpha-tubulin isotype M-alpha-4; alpha-tubulin isotype M-alpha-6; alpha-tubulin TUB1; alpha-tubulin ubiquitous; alphaTubulin84B; Alpha-Tubulin84B; ALS22; anon-EST:Liang-1.59; anon-EST:Liang-2.30; AT25469p; aTub84B; a-tub84B; bA408E5.3; B-ALPHA-1; bcm948; BEST:LD32507; cb944; CG1913; CG1913-PA; chr3R:2914276. 2914416; clone 1.59; clone 2.30; Detyrosinated tubulin alpha-1A chain; Detyrosinated tubulin alpha-1B chain; Detyrosinated tubulin alpha-1C chain; Detyrosinated tubulin alpha-3 chain; Detyrosinated tubulin alpha-3C chain; Detyrosinated tubulin alpha-3D chain; Detyrosinated tubulin alpha-3E chain; DM1alpha; Dmel CG1913; Dmel_CG1913; DTA1; fb22g06; FLJ30169; H2 ALPHA; H2-ALPHA; hum-a-tub1; hum-a-tub2; I79_019110; K-ALPHA-1; l(3)84Bd; l(3)g3; lis3; LOC100726777; LOC100731885; LOC100857858; LOC101118307; LOC106557476; M[a]4; M[a]6; ms(3)nc33; nc33; RGD1565476; similar to alpha-tubulin isoform 1; similar to tubulin alpha 1; T; Talpha1; Talpha1 alpha-tubulin; TBA1_DROME; Testis-specific alpha-tubulin; Tub; Tub 84B; TUB1; Tub1C; Tub84B; TUBA1; Tuba-1; tuba1 protein; TUBA1/3/4; Tuba1a; tuba1a.L; tuba1a-a; tuba1a-b; TUBA1B; TUBA1C; tuba1l; tuba1l2; TUBA2; TUBA3; TUBA3C; TUBA3D; TUBA3E; Tuba4; Tuba4a; TUBA5; Tuba6; tuba8.S; tubA84B; tubal2; Tubalpha1; tubulin; tubulin 1 alpha-chain; tubulin 1alpha; tubulin alpha; tubulin alpha 1a; tubulin alpha 1a L homeolog; tubulin alpha 1b; tubulin alpha 1c; tubulin alpha 3; tubulin alpha 3c; tubulin alpha 3e; tubulin alpha 4a; tubulin alpha 6; tubulin alpha 8 S homeolog; tubulin alpha1; tubulin alpha-1 chain; tubulin alpha1 promoter; Tubulin alpha-1A chain; tubulin alpha-1B chain; Tubulin alpha-1C chain; tubulin alpha-1C chain-like; tubulin alpha-2 chain; Tubulin alpha-3 chain; Tubulin alpha-3C chain; tubulin alpha-3C/D chain; Tubulin alpha-3D chain; Tubulin alpha-3E chain; tubulin alpha-4 chain; Tubulin alpha-4A chain; Tubulin alpha-5 chain; tubulin alpha-5 chain-like; tubulin alpha-6 chain; tubulin alpha-8 chain; tubulin alpha-ubiquitous chain; tubulin B-alpha-1; Tubulin H2-alpha; tubulin K-alpha-1; tubulin(alpha1); tubulin, alpha 1; tubulin, alpha 1 (testis specific); tubulin, alpha 1, like; tubulin, alpha 1, like 2; tubulin, alpha 1a; tubulin, alpha 1b; tubulin, alpha 1c; tubulin, alpha 2; tubulin, alpha 3c; tubulin, alpha 3e; tubulin, alpha 4; tubulin, alpha 4a; tubulin, alpha 5; tubulin, alpha 6; tubulin, alpha, brain-specific; tubulin, alpha, ubiquitous; tubulin1alpha; tubulin84B; Tubulin-alpha1; Unknown (protein for MGC:133894); unnamed protein product; wu:fb22g06; wu:fb37a10; XELAEV_18019849mg; XELAEV_18020901mg; YML085C

more info or order :

Invitrogen product webpage