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company name :
Invitrogen
other brands :
NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience
product type :
antibody
product name :
nNOS Polyclonal Antibody
catalog :
61-7000
quantity :
100 µg
price :
375 USD
clonality :
polyclonal
host :
domestic rabbit
conjugate :
nonconjugated
reactivity :
mouse, rat
application :
western blot, ELISA, immunohistochemistry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section, immunohistochemistry - free floating section
more info or order :
Invitrogen product webpage
citations: 19
Published Application/Species/Sample/DilutionReference
  • immunohistochemistry - frozen section; mouse; loading ...; fig s3a
Chen B, Xu C, Wang Y, Lin W, Wang Y, Chen L, et al. A disinhibitory nigra-parafascicular pathway amplifies seizure in temporal lobe epilepsy. Nat Commun. 2020;11:923 pubmed publisher
  • immunohistochemistry - free floating section; mouse; 1:500; loading ...; fig 5-s1b
Hachisuka J, Baumbauer K, Omori Y, Snyder L, Koerber H, Ross S. Semi-intact ex vivo approach to investigate spinal somatosensory circuits. elife. 2016;5: pubmed publisher
  • immunohistochemistry; mouse; 1:2000; fig 6
Farkas I, Vastagh C, Farkas E, Bálint F, Skrapits K, Hrabovszky E, et al. Glucagon-Like Peptide-1 Excites Firing and Increases GABAergic Miniature Postsynaptic Currents (mPSCs) in Gonadotropin-Releasing Hormone (GnRH) Neurons of the Male Mice via Activation of Nitric Oxide (NO) and Suppression of Endocannabinoid Signaling . Front Cell Neurosci. 2016;10:214 pubmed publisher
  • western blot; mouse; 1:250; loading ...; fig s1d
Mikami Y, Kanemaru K, Okubo Y, Nakaune T, Suzuki J, Shibata K, et al. Nitric Oxide-induced Activation of the Type 1 Ryanodine Receptor Is Critical for Epileptic Seizure-induced Neuronal Cell Death. EBioMedicine. 2016;11:253-261 pubmed publisher
  • immunohistochemistry - paraffin section; rat; fig 3
Takır S, Gürel Gürevin E, Toprak A, Demirci Tansel C, Uydeş Doğan B. The elevation of intraocular pressure is associated with apoptosis and increased immunoreactivity for nitric oxide synthase in rat retina whereas the effectiveness of retina derived relaxing factor is unaffected. Exp Eye Res. 2016;145:401-411 pubmed publisher
Jiménez Altayó F, Ortiz Romero P, Puertas Umbert L, Dantas A, Perez B, Vila E, et al. Stenosis coexists with compromised α1-adrenergic contractions in the ascending aorta of a mouse model of Williams-Beuren syndrome. Sci Rep. 2020;10:889 pubmed publisher
Pai E, Vogt D, Clemente Perez A, McKinsey G, Cho F, Hu J, et al. Mafb and c-Maf Have Prenatal Compensatory and Postnatal Antagonistic Roles in Cortical Interneuron Fate and Function. Cell Rep. 2019;26:1157-1173.e5 pubmed publisher
Jacoby J, Nath A, Jessen Z, Schwartz G. A Self-Regulating Gap Junction Network of Amacrine Cells Controls Nitric Oxide Release in the Retina. Neuron. 2018;100:1149-1162.e5 pubmed publisher
Blain A, Greally E, McClorey G, Manzano R, Betts C, Godfrey C, et al. Peptide-conjugated phosphodiamidate oligomer-mediated exon skipping has benefits for cardiac function in mdx and Cmah-/-mdx mouse models of Duchenne muscular dystrophy. PLoS ONE. 2018;13:e0198897 pubmed publisher
Meng D, Li H, Deisseroth K, Leutgeb S, Spitzer N. Neuronal activity regulates neurotransmitter switching in the adult brain following light-induced stress. Proc Natl Acad Sci U S A. 2018;115:5064-5071 pubmed publisher
Wang D, Tawfik V, Corder G, Low S, Francois A, Basbaum A, et al. Functional Divergence of Delta and Mu Opioid Receptor Organization in CNS Pain Circuits. Neuron. 2018;98:90-108.e5 pubmed publisher
Park S, Pottackal J, Ke J, Jun N, Rahmani P, Kim I, et al. Convergence and Divergence of CRH Amacrine Cells in Mouse Retinal Circuitry. J Neurosci. 2018;38:3753-3766 pubmed publisher
Schneider L, do Nascimento J, Trevizan A, Góis M, Borges S, Beraldi E, et al. Toxoplasma gondii promotes changes in VIPergic submucosal neurons, mucosal intraepithelial lymphocytes, and goblet cells during acute infection in the ileum of rats. Neurogastroenterol Motil. 2018;30:e13264 pubmed publisher
Sánchez Blázquez P, Pozo Rodrigálvarez A, Merlos M, Garzón J. The Sigma-1 Receptor Antagonist, S1RA, Reduces Stroke Damage, Ameliorates Post-Stroke Neurological Deficits and Suppresses the Overexpression of MMP-9. Mol Neurobiol. 2018;55:4940-4951 pubmed publisher
Paul A, Crow M, Raudales R, He M, Gillis J, Huang Z. Transcriptional Architecture of Synaptic Communication Delineates GABAergic Neuron Identity. Cell. 2017;171:522-539.e20 pubmed publisher
Ishino Y, Yetman M, Sossi S, Steinecke A, Hayano Y, Taniguchi H. Regional Cellular Environment Shapes Phenotypic Variations of Hippocampal and Neocortical Chandelier Cells. J Neurosci. 2017;37:9901-9916 pubmed publisher
Barnabei M, Sjaastad F, Townsend D, Bedada F, Metzger J. Severe dystrophic cardiomyopathy caused by the enteroviral protease 2A-mediated C-terminal dystrophin cleavage fragment. Sci Transl Med. 2015;7:294ra106 pubmed publisher
Stenkamp Strahm C, Nyavor Y, Kappmeyer A, Horton S, Gericke M, Balemba O. Prolonged high fat diet ingestion, obesity, and type 2 diabetes symptoms correlate with phenotypic plasticity in myenteric neurons and nerve damage in the mouse duodenum. Cell Tissue Res. 2015;361:411-26 pubmed publisher
Dittrich L, Morairty S, Warrier D, Kilduff T. Homeostatic sleep pressure is the primary factor for activation of cortical nNOS/NK1 neurons. Neuropsychopharmacology. 2015;40:632-9 pubmed publisher
product information
Product Type :
Antibody
Product Name :
nNOS Polyclonal Antibody
Catalog # :
61-7000
Quantity :
100 µg
Price :
375 USD
Clonality :
Polyclonal
Purity :
Antigen affinity chromatography
Host :
Rabbit
Reactivity :
Mouse, Rat
Applications :
ELISA: 0.1–1 µg/mL, Immunohistochemistry (Frozen): 1–2 µg/mL, Immunohistochemistry (Paraffin): 5-10 µg/mL, Immunohistochemistry: Assay Dependent, Western Blot: 1 µg/mL
Species :
Mouse, Rat
Isotype :
IgG
Storage :
-20°C
Description :
NOS activity requires homodimerization as well as three cosubstrates (L-arginine, NADPH and O2) and five cofactors or prosthetic groups (FAD, FMN, calmodulin, tetrahydrobiopterin and heme). Several distinct NOS isoforms have been described and been shown to represent the products of three distinct genes. These include two constitutive Ca2+/CaM-dependent forms of NOS, including NOS1 (also designated ncNOS) whose activity was first identified in neurons, and NOS3 (also designated ecNOS), first identified in endothelial cells.
Immunogen :
A recombinant protein consisting of 195 amino acids from the N-terminus of rat nNOS protein.
Format :
Liquid
Applications w/Dilutions :
ELISA: 0.1–1 µg/mL, Immunohistochemistry (Frozen): 1–2 µg/mL, Immunohistochemistry (Paraffin): 5-10 µg/mL, Immunohistochemistry: Assay Dependent, Western Blot: 1 µg/mL
Aliases :
2310005C01Rik; Bnos; bNOS1; Brain NOS; constitutive NOS; IHPS1; NC-NOS; neuronal nitric oxide synthase; Neuronal NOS; nitric oxidase synthase; nitric oxide synthase; nitric oxide synthase 1; nitric oxide synthase 1 (neuronal); nitric oxide synthase 1, neuronal; nitric oxide synthase, brain; nNOS; N-NOS; nNOS; NOS type I; NO; NOS; NOS Type 1; NOS type I; Nos1; Nos-1; NOS-I; peptidyl-cysteine S-nitrosylase NOS1; Phospho-bNOS; Phospho-Brain NOS
more info or order :
Invitrogen product webpage
company information
Invitrogen
Thermo Fisher Scientific
81 Wyman Street
Waltham, MA USA 02451
https://www.thermofisher.com
800-678-5599
headquarters: USA