This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Invitrogen
other brands :
NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience
product type :
antibody
product name :
CD45R (B220) Monoclonal Antibody (RA3-6B2), Alexa Fluor 532, eBioscience
catalog :
58-0452-82
quantity :
100 ug
price :
US 401.00
clonality :
monoclonal
host :
rat
conjugate :
Alexa Fluor 532
clone name :
RA3-6B2

The same clone is also sold as:
reactivity :
human, mouse
application :
immunohistochemistry, flow cytometry, immunohistochemistry - paraffin section
citations: 45
Reference
Wolf M, Madden M, Arner E, Bader J, Ye X, Vlach L, et al. VHL loss reprograms the immune landscape to promote an inflammatory myeloid microenvironment in renal tumorigenesis. J Clin Invest. 2024;134: pubmed publisher
Vos W, van Os B, den Toom M, Beckers L, van Roomen C, Van Tiel C, et al. T cell specific deletion of Casitas B lineage lymphoma-b reduces atherosclerosis, but increases plaque T cell infiltration and systemic T cell activation. Front Immunol. 2024;15:1297893 pubmed publisher
Li F, Liang Z, Zhong H, Hu X, Tang Z, Zhu C, et al. Group 3 Innate Lymphoid Cells Exacerbate Lupus Nephritis by Promoting B Cell Activation in Kidney Ectopic Lymphoid Structures. Adv Sci (Weinh). 2023;10:e2302804 pubmed publisher
Schroer A, Ventura P, Sucharov J, Misra R, Chui M, Bieri G, et al. Platelet factors attenuate inflammation and rescue cognition in ageing. Nature. 2023;620:1071-1079 pubmed publisher
Sun Q, Wang Y, Ji H, Sun X, Xie S, Chen L, et al. Lenvatinib for effectively treating antiangiogenic drug-resistant nasopharyngeal carcinoma. Cell Death Dis. 2022;13:724 pubmed publisher
Zhong L, Yao L, Holdreith N, Yu W, Gui T, Miao Z, et al. Transient expansion and myofibroblast conversion of adipogenic lineage precursors mediate bone marrow repair after radiation. JCI Insight. 2022;7: pubmed publisher
Mart xed n Hern xe1 ndez D, Guti xe9 rrez I, Gonz xe1 lez Prieto M, Macdowell K, Robledo Monta xf1 a J, Tendilla Beltr xe1 n H, et al. Sphk2 deletion is involved in structural abnormalities and Th17 response but does not aggravate colon inflammation induced by sub-chronic stress. Sci Rep. 2022;12:4073 pubmed publisher
Ma L, Tang Q, Gao X, Lee J, Lei R, Suzuki M, et al. Tet-mediated DNA demethylation regulates specification of hematopoietic stem and progenitor cells during mammalian embryogenesis. Sci Adv. 2022;8:eabm3470 pubmed publisher
Guo L, Li N, Yang Z, Li H, Zheng H, Yang J, et al. Role of CXCL5 in Regulating Chemotaxis of Innate and Adaptive Leukocytes in Infected Lungs Upon Pulmonary Influenza Infection. Front Immunol. 2021;12:785457 pubmed publisher
Wilfahrt D, Philips R, Lama J, Kizerwetter M, Shapiro M, McCue S, et al. Histone deacetylase 3 represses cholesterol efflux during CD4+ T-cell activation. elife. 2021;10: pubmed publisher
Shen Y, Jiang L, Iyer V, Raposo B, Dubnovitsky A, Boddul S, et al. A rapid CRISPR competitive assay for in vitro and in vivo discovery of potential drug targets affecting the hematopoietic system. Comput Struct Biotechnol J. 2021;19:5360-5370 pubmed publisher
Hill D, Whyte C, Innocentin S, Lee J, Dooley J, Wang J, et al. Impaired HA-specific T follicular helper cell and antibody responses to influenza vaccination are linked to inflammation in humans. elife. 2021;10: pubmed publisher
Zhang Y, Paynter J, Sou C, Fourfouris T, Wang Y, Abraham C, et al. Mechanism of a COVID-19 nanoparticle vaccine candidate that elicits a broadly neutralizing antibody response to SARS-CoV-2 variants. Sci Adv. 2021;7:eabj3107 pubmed publisher
Jeanne A, Sarazin T, Charlé M, Moali C, Fichel C, Boulagnon Rombi C, et al. Targeting Ovarian Carcinoma with TSP-1:CD47 Antagonist TAX2 Activates Anti-Tumor Immunity. Cancers (Basel). 2021;13: pubmed publisher
Sudo K, Yamazaki S, Wilkinson A, Nakauchi H, Nakamura Y. Polyvinyl alcohol hydrolysis rate and molecular weight influence human and murine HSC activity ex vivo. Stem Cell Res. 2021;56:102531 pubmed publisher
Zhao Y, Cheng R, Zhao Y, Ge W, Yang Y, Ding Z, et al. Type 2 diabetic mice enter a state of spontaneous hibernation-like suspended animation following accumulation of uric acid. J Biol Chem. 2021;297:101166 pubmed publisher
Wilson C, Stocks B, Hoopes E, Rhoads J, McNew K, Major A, et al. Metabolic preconditioning in CD4+ T cells restores inducible immune tolerance in lupus-prone mice. JCI Insight. 2021;6: pubmed publisher
Belliere J, Casemayou A, Colliou E, El Hachem H, Kounde C, Piedrafita A, et al. Ibrutinib does not prevent kidney fibrosis following acute and chronic injury. Sci Rep. 2021;11:11985 pubmed publisher
Ho T, Dellorusso P, Verovskaya E, Bakker S, Flach J, Smith L, et al. Aged hematopoietic stem cells are refractory to bloodborne systemic rejuvenation interventions. J Exp Med. 2021;218: pubmed publisher
Zaccagnini G, Greco S, Longo M, Maimone B, Voellenkle C, Fuschi P, et al. Hypoxia-induced miR-210 modulates the inflammatory response and fibrosis upon acute ischemia. Cell Death Dis. 2021;12:435 pubmed publisher
Shang M, Yang H, Yang R, Chen T, Fu Y, Li Y, et al. The folate cycle enzyme MTHFD2 induces cancer immune evasion through PD-L1 up-regulation. Nat Commun. 2021;12:1940 pubmed publisher
Dobosz E, Lorenz G, Ribeiro A, Würf V, Wadowska M, Kotlinowski J, et al. Murine myeloid cell MCPIP1 suppresses autoimmunity by regulating B-cell expansion and differentiation. Dis Model Mech. 2021;14: pubmed publisher
Gaurav R, Mikuls T, Thiele G, Nelson A, Niu M, Guda C, et al. High-throughput analysis of lung immune cells in a combined murine model of agriculture dust-triggered airway inflammation with rheumatoid arthritis. PLoS ONE. 2021;16:e0240707 pubmed publisher
Mendes A, Gigan J, Rodríguez Rodrígues C, Choteau S, Sanséau D, Barros D, et al. Proteostasis in dendritic cells is controlled by the PERK signaling axis independently of ATF4. Life Sci Alliance. 2021;4: pubmed publisher
Larson S, Bortell N, Illies A, Crisler W, Matsuda J, Lenz L. Myeloid Cell CK2 Regulates Inflammation and Resistance to Bacterial Infection. Front Immunol. 2020;11:590266 pubmed publisher
Lederer K, Castaño D, Gomez Atria D, Oguin T, Wang S, Manzoni T, et al. SARS-CoV-2 mRNA Vaccines Foster Potent Antigen-Specific Germinal Center Responses Associated with Neutralizing Antibody Generation. Immunity. 2020;53:1281-1295.e5 pubmed publisher
Sen Kilic E, Blackwood C, Huckaby A, Horspool A, Weaver K, Malkowski A, et al. Defining the Mechanistic Correlates of Protection Conferred by Whole-Cell Vaccination against Pseudomonas aeruginosa Acute Murine Pneumonia. Infect Immun. 2021;89: pubmed publisher
Aung K, Akiyama K, Kunitomo M, Mun A, Tosa I, Nguyen H, et al. Aging-Affected MSC Functions and Severity of Periodontal Tissue Destruction in a Ligature-Induced Mouse Periodontitis Model. Int J Mol Sci. 2020;21: pubmed publisher
Myers D, Abram C, Wildes D, Belwafa A, Welsh A, Schulze C, et al. Shp1 Loss Enhances Macrophage Effector Function and Promotes Anti-Tumor Immunity. Front Immunol. 2020;11:576310 pubmed publisher
Liu Z, Gu Y, Shin A, Zhang S, Ginhoux F. Analysis of Myeloid Cells in Mouse Tissues with Flow Cytometry. STAR Protoc. 2020;1:100029 pubmed publisher
Sinha D, Nag P, Nanayakkara D, Duijf P, Burgess A, Raninga P, et al. Cep55 overexpression promotes genomic instability and tumorigenesis in mice. Commun Biol. 2020;3:593 pubmed publisher
Heimesaat M, Mousavi S, Kl xf8 ve S, Genger C, Weschka D, Tamas A, et al. Pituitary Adenylate Cyclase-Activating Polypeptide Alleviates Intestinal, Extra-Intestinal and Systemic Inflammatory Responses during Acute Campylobacter jejuni-induced Enterocolitis in Mice. Pathogens. 2020;9: pubmed publisher
Heimesaat M, Genger C, Klove S, Weschka D, Mousavi S, Bereswill S. The Host-Specific Intestinal Microbiota Composition Impacts Campylobacter coli Infection in a Clinical Mouse Model of Campylobacteriosis. Pathogens. 2020;9: pubmed publisher
Richmond B, Mansouri S, Serezani A, Novitskiy S, Blackburn J, Du R, et al. Monocyte-derived dendritic cells link localized secretory IgA deficiency to adaptive immune activation in COPD. Mucosal Immunol. 2021;14:431-442 pubmed publisher
Naka K, Ochiai R, Matsubara E, Kondo C, Yang K, Hoshii T, et al. The lysophospholipase D enzyme Gdpd3 is required to maintain chronic myelogenous leukaemia stem cells. Nat Commun. 2020;11:4681 pubmed publisher
Kreimann K, Jang M, Rong S, Greite R, von Vietinghoff S, Schmitt R, et al. Ischemia Reperfusion Injury Triggers CXCL13 Release and B-Cell Recruitment After Allogenic Kidney Transplantation. Front Immunol. 2020;11:1204 pubmed publisher
Kawabe T, Yi J, Kawajiri A, Hilligan K, Fang D, Ishii N, et al. Requirements for the differentiation of innate T-bethigh memory-phenotype CD4+ T lymphocytes under steady state. Nat Commun. 2020;11:3366 pubmed publisher
Cao H, Duan R, Hu J. Overcoming Immunological Challenges to Helper-Dependent Adenoviral Vector-Mediated Long-Term CFTR Expression in Mouse Airways. Genes (Basel). 2020;11: pubmed publisher
Kløve S, Genger C, Mousavi S, Weschka D, Bereswill S, Heimesaat M. Toll-Like Receptor-4 Dependent Intestinal and Systemic Sequelae Following Peroral Campylobacter coli Infection of IL10 Deficient Mice Harboring a Human Gut Microbiota. Pathogens. 2020;9: pubmed publisher
Natsume C, Aoki N, Aoyama T, Senda K, Matsui M, Ikegami A, et al. Fucoxanthin Ameliorates Atopic Dermatitis Symptoms by Regulating Keratinocytes and Regulatory Innate Lymphoid Cells. Int J Mol Sci. 2020;21: pubmed publisher
Kang Y, Pietras E, Passegue E. Deregulated Notch and Wnt signaling activates early-stage myeloid regeneration pathways in leukemia. J Exp Med. 2020;217: pubmed publisher
Núñez M, Wisser K, Veatch S. Synergistic factors control kinase-phosphatase organization in B-cells engaged with supported bilayers. Mol Biol Cell. 2020;31:667-682 pubmed publisher
Ramakrishna C, Kujawski M, Chu H, Li L, Mazmanian S, CANTIN E. Bacteroides fragilis polysaccharide A induces IL-10 secreting B and T cells that prevent viral encephalitis. Nat Commun. 2019;10:2153 pubmed publisher
Adane B, Ye H, Khan N, Pei S, Minhajuddin M, Stevens B, et al. The Hematopoietic Oxidase NOX2 Regulates Self-Renewal of Leukemic Stem Cells. Cell Rep. 2019;27:238-254.e6 pubmed publisher
Ziegler P, Bollrath J, Pallangyo C, Matsutani T, Canli O, De Oliveira T, et al. Mitophagy in Intestinal Epithelial Cells Triggers Adaptive Immunity during Tumorigenesis. Cell. 2018;174:88-101.e16 pubmed publisher
product information
Product Type :
Antibody
Product Name :
CD45R (B220) Monoclonal Antibody (RA3-6B2), Alexa Fluor 532, eBioscience
Catalog # :
58-0452-82
Quantity :
100 ug
Price :
US 401.00
Clonality :
Monoclonal
Purity :
Affinity chromatography
Host :
Rat
Reactivity :
Human, Mouse
Applications :
Flow Cytometry: 0.25 ug/test, Immunohistochemistry (Paraffin): 1:100
Species :
Human, Mouse
Clone :
RA3-6B2
Isotype :
IgG2a, kappa
Storage :
4 C, store in dark, DO NOT FREEZE!
Description :
CD45R (PTPRC) is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. CD45 contains an extracellular domain, a single transmembrane segment and two tandem intracytoplasmic catalytic domains. CD45 is specifically expressed in hematopoietic cells and has been shown to be an essential regulator of T- and B-cell antigen receptor signaling. CD45 glycoprotein is crucial in lymphocyte development and antigen signaling, serving as an important regulator of Src-family kinases. CD45 protein exists in multiple isoforms as a result of alternative splicing, and these isoforms differ in their extracellular domains, but share identical transmembrane and cytoplasmic domains. Further, these CD45 isoforms differ in their ability to translocate into the glycosphingolipid-enriched membrane domains and their expression depends on cell type and physiological state of the cell. In addition to its role in immunoreceptor signaling, CD45 is important in promoting cell survival by modulating integrin-mediated signal transduction pathway and is also involved in DNA fragmentation during apoptosis. CD45RA is an isoform of the CD45 complex and has restricted expression between different subtypes of lymphoid cells. Diseases associated with CD45 dysfunction include Severe Combined Immunodeficiency (T-cell negative, B-Cell Negative) and Hepatitis C.
Format :
Liquid
Applications w/Dilutions :
Flow Cytometry: 0.25 ug/test, Immunohistochemistry (Paraffin): 1:100
Aliases :
B220; Cd45; CD45 antigen; CD45R; GP180; Lca; L-CA; leucocyte common antigen; leukocyte common antigen; leukocyte common antigen A; leukocyte common antigen B; loc; LY5; Ly-5; Lymphocyte antigen 5; lymphocyte common antigen; Lyt-4; membrane tyrosine phosphatase; protein tyrosine phosphatase receptor type C; protein tyrosine phosphatase, receptor type C; protein tyrosine phosphatase, receptor type, C; protein tyrosine phosphatase, receptor type, c polypeptide; Protein tyrosine phosphatase, receptor-type, c polypeptide; Ptprc; Receptor-type tyrosine-protein phosphatase C; RT7; T200; T200 glycoprotein; T200 leukocyte common antigen
company information
Invitrogen
Thermo Fisher Scientific
81 Wyman Street
Waltham, MA USA 02451
https://www.thermofisher.com
800-678-5599
headquarters: USA