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company name :
Invitrogen
other brands :
NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience
product type :
antibody
product name :
CD45.2 Monoclonal Antibody (104), Alexa Fluor 700, eBioscience™
catalog :
56-0454-81
quantity :
50 µg
price :
US 172
clonality :
monoclonal
host :
mouse
conjugate :
AF700
clone name :
104
reactivity :
mouse
application :
flow cytometry
more info or order :
citations: 74
Published Application/Species/Sample/DilutionReference
  • flow cytometry; mouse; loading ...; fig 1c
Baumgartner C, Toifl S, Farlik M, Halbritter F, Scheicher R, Fischer I, et al. An ERK-Dependent Feedback Mechanism Prevents Hematopoietic Stem Cell Exhaustion. Cell Stem Cell. 2018;22:879-892.e6 pubmed publisher
  • flow cytometry; mouse; 1:200; loading ...; fig 5d, 5i
Campana L, Starkey Lewis P, Pellicoro A, Aucott R, Man J, O Duibhir E, et al. The STAT3-IL-10-IL-6 Pathway Is a Novel Regulator of Macrophage Efferocytosis and Phenotypic Conversion in Sterile Liver Injury. J Immunol. 2018;200:1169-1187 pubmed publisher
  • flow cytometry; mouse; 1:200; loading ...; fig 5a
Zamarin D, Holmgaard R, Ricca J, Plitt T, Palese P, Sharma P, et al. Intratumoral modulation of the inducible co-stimulator ICOS by recombinant oncolytic virus promotes systemic anti-tumour immunity. Nat Commun. 2017;8:14340 pubmed publisher
  • flow cytometry; mouse; fig s2
Damgaard R, Walker J, Marco Casanova P, Morgan N, Titheradge H, Elliott P, et al. The Deubiquitinase OTULIN Is an Essential Negative Regulator of Inflammation and Autoimmunity. Cell. 2016;166:1215-1230.e20 pubmed publisher
  • flow cytometry; mouse; fig 1
Hu W, Dooley J, Chung S, Chandramohan D, Cimmino L, Mukherjee S, et al. miR-29a maintains mouse hematopoietic stem cell self-renewal by regulating Dnmt3a. Blood. 2015;125:2206-16 pubmed publisher
  • flow cytometry; mouse
Cowan J, McCarthy N, Parnell S, White A, Bacon A, Serge A, et al. Differential requirement for CCR4 and CCR7 during the development of innate and adaptive ??T cells in the adult thymus. J Immunol. 2014;193:1204-12 pubmed publisher
  • flow cytometry; mouse
Povinelli B, Nemeth M. Wnt5a regulates hematopoietic stem cell proliferation and repopulation through the Ryk receptor. Stem Cells. 2014;32:105-15 pubmed publisher
  • flow cytometry; mouse; fig 6b
Wu X, Satpathy A, Kc W, Liu P, Murphy T, Murphy K. Bcl11a controls Flt3 expression in early hematopoietic progenitors and is required for pDC development in vivo. PLoS ONE. 2013;8:e64800 pubmed publisher
Guilliams M, Bonnardel J, Haest B, Vanderborght B, Wagner C, Remmerie A, et al. Spatial proteogenomics reveals distinct and evolutionarily conserved hepatic macrophage niches. Cell. 2022;185:379-396.e38 pubmed publisher
Young I, Wu B, Andricovich J, Chuang S, Li R, Tzatsos A, et al. Differentiation of fetal hematopoietic stem cells requires ARID4B to restrict autocrine KITLG/KIT-Src signaling. Cell Rep. 2021;37:110036 pubmed publisher
Gerber A, Abdi K, Singh N. The subunits of IL-12, originating from two distinct cells, can functionally synergize to protect against pathogen dissemination in vivo. Cell Rep. 2021;37:109816 pubmed publisher
Alvim R, Georgala P, Nogueira L, Somma A, Nagar K, Thomas J, et al. Combined OX40 Agonist and PD-1 Inhibitor Immunotherapy Improves the Efficacy of Vascular Targeted Photodynamic Therapy in a Urothelial Tumor Model. Molecules. 2021;26: pubmed publisher
Kawano R, Okamura T, Hashimoto Y, Majima S, Senmaru T, Ushigome E, et al. Erythritol Ameliorates Small Intestinal Inflammation Induced by High-Fat Diets and Improves Glucose Tolerance. Int J Mol Sci. 2021;22: pubmed publisher
Prasad M, Wojciech L, Brzostek J, Hu J, Chua Y, Tung D, et al. Expansion of an Unusual Virtual Memory CD8+ Subpopulation Bearing Vα3.2 TCR in Themis-Deficient Mice. Front Immunol. 2021;12:644483 pubmed publisher
Hu X, Dong X, Li G, Chen Y, Chen J, He X, et al. Brd4 modulates diet-induced obesity via PPARγ-dependent Gdf3 expression in adipose tissue macrophages. JCI Insight. 2021;6: pubmed publisher
Moon S, Park Y, Hyeon S, Kim Y, Kim J, Kim H, et al. Niche-specific MHC II and PD-L1 regulate CD4+CD8αα+ intraepithelial lymphocyte differentiation. J Exp Med. 2021;218: pubmed publisher
Rivas M, Meydan C, Chin C, Challman M, Kim D, Bhinder B, et al. Smc3 dosage regulates B cell transit through germinal centers and restricts their malignant transformation. Nat Immunol. 2021;22:240-253 pubmed publisher
Wei S, Meijers W, Axelrod M, Anang N, Screever E, Wescott E, et al. A Genetic Mouse Model Recapitulates Immune Checkpoint Inhibitor-Associated Myocarditis and Supports a Mechanism-Based Therapeutic Intervention. Cancer Discov. 2021;11:614-625 pubmed publisher
Baniahmad A, Birkner K, Görg J, Loos J, Zipp F, Wasser B, et al. The frequency of follicular T helper cells differs in acute and chronic neuroinflammation. Sci Rep. 2020;10:20485 pubmed publisher
Fu H, Sun Y, Shao Y, Saredy J, Cueto R, Liu L, et al. Interleukin 35 Delays Hindlimb Ischemia-Induced Angiogenesis Through Regulating ROS-Extracellular Matrix but Spares Later Regenerative Angiogenesis. Front Immunol. 2020;11:595813 pubmed publisher
Remmerie A, Martens L, Thoné T, Castoldi A, Seurinck R, Pavie B, et al. Osteopontin Expression Identifies a Subset of Recruited Macrophages Distinct from Kupffer Cells in the Fatty Liver. Immunity. 2020;53:641-657.e14 pubmed publisher
Morris M, Ricart Arbona R, Daniels K, Gardner R, Easthausen I, Boteler W, et al. Mite Burden and Immunophenotypic Response to Demodex musculi in Swiss Webster, BALB/c, C57BL/6, and NSG Mice. Comp Med. 2020;70:336-348 pubmed publisher
McElroy G, Reczek C, Reyfman P, Mithal D, Horbinski C, Chandel N. NAD+ Regeneration Rescues Lifespan, but Not Ataxia, in a Mouse Model of Brain Mitochondrial Complex I Dysfunction. Cell Metab. 2020;32:301-308.e6 pubmed publisher
Bosteels C, Neyt K, Vanheerswynghels M, Van Helden M, Sichien D, Debeuf N, et al. Inflammatory Type 2 cDCs Acquire Features of cDC1s and Macrophages to Orchestrate Immunity to Respiratory Virus Infection. Immunity. 2020;52:1039-1056.e9 pubmed publisher
Wang X, Ni L, Wan S, Zhao X, Ding X, Dejean A, et al. Febrile Temperature Critically Controls the Differentiation and Pathogenicity of T Helper 17 Cells. Immunity. 2020;52:328-341.e5 pubmed publisher
Gao J, Owusu Ansah A, Paun A, Beacht K, Yim E, Siwicki M, et al. Low-level Cxcr4-haploinsufficient HSC engraftment is sufficient to correct leukopenia in WHIM syndrome mice. JCI Insight. 2019;4: pubmed publisher
Cheng Y, Luo H, Izzo F, Pickering B, Nguyen D, Myers R, et al. m6A RNA Methylation Maintains Hematopoietic Stem Cell Identity and Symmetric Commitment. Cell Rep. 2019;28:1703-1716.e6 pubmed publisher
Wilkinson A, Ishida R, Kikuchi M, Sudo K, Morita M, Crisostomo R, et al. Long-term ex vivo haematopoietic-stem-cell expansion allows nonconditioned transplantation. Nature. 2019;: pubmed publisher
Walker J, Clark P, Crisp A, Barlow J, Szeto A, Ferreira A, et al. Polychromic Reporter Mice Reveal Unappreciated Innate Lymphoid Cell Progenitor Heterogeneity and Elusive ILC3 Progenitors in Bone Marrow. Immunity. 2019;: pubmed publisher
Shikatani E, Besla R, Ensan S, Upadhye A, Khyzha N, Li A, et al. c-Myb Exacerbates Atherosclerosis through Regulation of Protective IgM-Producing Antibody-Secreting Cells. Cell Rep. 2019;27:2304-2312.e6 pubmed publisher
Wei S, Sharma R, Anang N, Levine J, Zhao Y, Mancuso J, et al. Negative Co-stimulation Constrains T Cell Differentiation by Imposing Boundaries on Possible Cell States. Immunity. 2019;50:1084-1098.e10 pubmed publisher
Math xe4 L, Shim H, Steer C, Yin Y, Martínez González I, Takei F. Female and male mouse lung group 2 innate lymphoid cells differ in gene expression profiles and cytokine production. PLoS ONE. 2019;14:e0214286 pubmed publisher
Kesarwani M, Kincaid Z, Azam M. Methods for Evaluating the Role of c-Fos and Dusp1 in Oncogene Dependence. J Vis Exp. 2019;: pubmed publisher
Chen L, Gu J, Qian Y, Li M, Qian Y, Xu M, et al. Deletion of C-C Motif Chemokine Ligand 5 Worsens Invariant Natural Killer T-Cell-Mediated Hepatitis via Compensatory Up-regulation of CXCR2-Related Chemokine Activity. Cell Mol Gastroenterol Hepatol. 2019;7:623-639 pubmed publisher
Moretti F, Klapproth S, Ruppert R, Margraf A, Weber J, Pick R, et al. Differential requirement of kindlin-3 for T cell progenitor homing to the non-vascularized and vascularized thymus. elife. 2018;7: pubmed publisher
Oseledchyk A, Ricca J, Gigoux M, Ko B, Redelman Sidi G, Walther T, et al. Lysis-independent potentiation of immune checkpoint blockade by oncolytic virus. Oncotarget. 2018;9:28702-28716 pubmed publisher
Schmidt K, Zhang Q, Tasdogan A, Petzold A, Dahl A, Arneth B, et al. The H3K4 methyltransferase Setd1b is essential for hematopoietic stem and progenitor cell homeostasis in mice. elife. 2018;7: pubmed publisher
Wilson K, Ochoa L, Solomon O, Pal R, Cardona S, Carpio V, et al. Elimination of intravascular thrombi prevents early mortality and reduces gliosis in hyper-inflammatory experimental cerebral malaria. J Neuroinflammation. 2018;15:173 pubmed publisher
Zamarin D, Ricca J, Sadekova S, Oseledchyk A, Yu Y, Blumenschein W, et al. PD-L1 in tumor microenvironment mediates resistance to oncolytic immunotherapy. J Clin Invest. 2018;128:1413-1428 pubmed publisher
Ricca J, Oseledchyk A, Walther T, Liu C, Mangarin L, Merghoub T, et al. Pre-existing Immunity to Oncolytic Virus Potentiates Its Immunotherapeutic Efficacy. Mol Ther. 2018;26:1008-1019 pubmed publisher
Jee D, Yang J, Park S, Farmer D, Wen J, Chou T, et al. Dual Strategies for Argonaute2-Mediated Biogenesis of Erythroid miRNAs Underlie Conserved Requirements for Slicing in Mammals. Mol Cell. 2018;69:265-278.e6 pubmed publisher
Guo X, Chu L, Ke F, Mu L, Li Z, Cai J, et al. Recipient bone marrow assimilates the myeloid/lymphoid reconstitution of distinct fetal hematopoietic stem cells. Oncotarget. 2017;8:108981-108988 pubmed publisher
Cioncada R, Maddaluno M, Vo H, Woodruff M, Tavarini S, Sammicheli C, et al. Vaccine adjuvant MF59 promotes the intranodal differentiation of antigen-loaded and activated monocyte-derived dendritic cells. PLoS ONE. 2017;12:e0185843 pubmed publisher
Zaid A, Hor J, Christo S, Groom J, Heath W, Mackay L, et al. Chemokine Receptor-Dependent Control of Skin Tissue-Resident Memory T Cell Formation. J Immunol. 2017;199:2451-2459 pubmed publisher
Nair S, Poddar S, Shimak R, Diamond M. Interferon Regulatory Factor 1 Protects against Chikungunya Virus-Induced Immunopathology by Restricting Infection in Muscle Cells. J Virol. 2017;91: pubmed publisher
Xu J, Guardado J, Hoffman R, Xu H, Namas R, Vodovotz Y, et al. IL33-mediated ILC2 activation and neutrophil IL5 production in the lung response after severe trauma: A reverse translation study from a human cohort to a mouse trauma model. PLoS Med. 2017;14:e1002365 pubmed publisher
Giles D, Moreno Fernandez M, Stankiewicz T, Graspeuntner S, Cappelletti M, Wu D, et al. Thermoneutral housing exacerbates nonalcoholic fatty liver disease in mice and allows for sex-independent disease modeling. Nat Med. 2017;23:829-838 pubmed publisher
Miao T, Symonds A, Singh R, Symonds J, Ogbe A, Omodho B, et al. Egr2 and 3 control adaptive immune responses by temporally uncoupling expansion from T cell differentiation. J Exp Med. 2017;214:1787-1808 pubmed publisher
Eddens T, Elsegeiny W, Garcia Hernadez M, Castillo P, Trevejo Nuñez G, Serody K, et al. Pneumocystis-Driven Inducible Bronchus-Associated Lymphoid Tissue Formation Requires Th2 and Th17 Immunity. Cell Rep. 2017;18:3078-3090 pubmed publisher
Hérault A, Binnewies M, Leong S, Calero Nieto F, Zhang S, Kang Y, et al. Myeloid progenitor cluster formation drives emergency and leukaemic myelopoiesis. Nature. 2017;544:53-58 pubmed publisher
Ambrosi T, Scialdone A, Graja A, Gohlke S, Jank A, Bocian C, et al. Adipocyte Accumulation in the Bone Marrow during Obesity and Aging Impairs Stem Cell-Based Hematopoietic and Bone Regeneration. Cell Stem Cell. 2017;20:771-784.e6 pubmed publisher
Lucinda N, Figueiredo M, Pessoa N, Santos B, Lima G, Freitas A, et al. Dendritic cells, macrophages, NK and CD8+ T lymphocytes play pivotal roles in controlling HSV-1 in the trigeminal ganglia by producing IL1-beta, iNOS and granzyme B. Virol J. 2017;14:37 pubmed publisher
Deken M, Gadiot J, Jordanova E, Lacroix R, van Gool M, Kroon P, et al. Targeting the MAPK and PI3K pathways in combination with PD1 blockade in melanoma. Oncoimmunology. 2016;5:e1238557 pubmed publisher
Chudnovskiy A, Mortha A, Kana V, Kennard A, Ramírez J, Rahman A, et al. Host-Protozoan Interactions Protect from Mucosal Infections through Activation of the Inflammasome. Cell. 2016;167:444-456.e14 pubmed publisher
Shi Y, Wu W, Chai Q, Li Q, Hou Y, Xia H, et al. LTβR controls thymic portal endothelial cells for haematopoietic progenitor cell homing and T-cell regeneration. Nat Commun. 2016;7:12369 pubmed publisher
Mucci A, Kunkiel J, Suzuki T, Brennig S, Glage S, Kühnel M, et al. Murine iPSC-Derived Macrophages as a Tool for Disease Modeling of Hereditary Pulmonary Alveolar Proteinosis due to Csf2rb Deficiency. Stem Cell Reports. 2016;7:292-305 pubmed publisher
Giles D, Moreno Fernandez M, Stankiewicz T, Cappelletti M, Huppert S, Iwakura Y, et al. Regulation of Inflammation by IL-17A and IL-17F Modulates Non-Alcoholic Fatty Liver Disease Pathogenesis. PLoS ONE. 2016;11:e0149783 pubmed publisher
DU Four S, Maenhout S, De Pierre K, Renmans D, Niclou S, Thielemans K, et al. Axitinib increases the infiltration of immune cells and reduces the suppressive capacity of monocytic MDSCs in an intracranial mouse melanoma model. Oncoimmunology. 2015;4:e998107 pubmed
Wan W, Liu Q, Lionakis M, Marino A, Anderson S, Swamydas M, et al. Atypical chemokine receptor 1 deficiency reduces atherogenesis in ApoE-knockout mice. Cardiovasc Res. 2015;106:478-87 pubmed publisher
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product information
Product Type :
Antibody
Product Name :
CD45.2 Monoclonal Antibody (104), Alexa Fluor 700, eBioscience™
Catalog # :
56-0454-81
Quantity :
50 µg
Price :
US 172
Clonality :
Monoclonal
Purity :
Affinity chromatography
Host :
Mouse
Reactivity :
Mouse
Applications :
Flow Cytometry: 0.5 µg/test
Species :
Mouse
Clone :
104
Isotype :
IgG2a, kappa
Storage :
4° C, store in dark, DO NOT FREEZE!
Description :
CD45.2 (LCA, leukocyte common antigen) is a receptor-type protein tyrosine phosphatase ubiquitously expressed in all nucleated hematopoietic cells, comprising approximately 10% of all surface proteins in lymphocytes. CD45.2 glycoprotein is crucial in lymphocyte development and antigen signaling, serving as an important regulator of Src-family kinases. CD45.2 protein exists as multiple isoforms as a result of alternative splicing; these isoforms differ in their extracellular domains, whereas they share identical transmembrane and cytoplasmic domains. These isoforms differ in their ability to translocate into the glycosphingolipid-enriched membrane domains and their expression depends on cell type and physiological state of the cell. Besides the role in immunoreceptor signaling, CD45.2 is important in promoting cell survival by modulating integrin-mediated signal transduction pathway and is also involved in DNA fragmentation during apoptosis. CD45RA is an isoform of the CD45 complex and has restricted expression between different subtypes of lymphoid cells.
Format :
Liquid
Applications w/Dilutions :
Flow Cytometry: 0.5 µg/test
Aliases :
B220; Cd45; CD45 antigen; CD45R; GP180; LCA; L-CA; Leukocyte common antigen; loc; LY5; Ly-5; Lymphocyte antigen 5; lymphocyte common antigen; Lyt-4; protein tyrosine phosphatase, receptor type, C; Ptprc; Receptor-type tyrosine-protein phosphatase C; T200; T200 glycoprotein
more info or order :
company information
Invitrogen
Thermo Fisher Scientific
81 Wyman Street
Waltham, MA USA 02451
https://www.thermofisher.com
800-678-5599
headquarters: USA