product summary
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company name :
Invitrogen
other brands :
NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience
product type :
antibody
product name :
CD45 Monoclonal Antibody (30-F11), Alexa Fluor 700, eBioscience™
catalog :
56-0451-80
quantity :
25 µg
price :
US 130
clonality :
monoclonal
host :
rat
conjugate :
AF700
clone name :
30-F11
reactivity :
mouse
application :
flow cytometry
more info or order :
citations: 349
Published Application/Species/Sample/DilutionReference
  • flow cytometry; mouse; 1:350; loading ...; fig 2f, 4b
Moreira T, Mangani D, Cox L, Leibowitz J, Lobo E, Oliveira M, et al. PD-L1+ and XCR1+ dendritic cells are region-specific regulators of gut homeostasis. Nat Commun. 2021;12:4907 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 1a
Thomson C, van de Pavert S, Stakenborg M, Labeeuw E, Matteoli G, Mowat A, et al. Expression of the Atypical Chemokine Receptor ACKR4 Identifies a Novel Population of Intestinal Submucosal Fibroblasts That Preferentially Expresses Endothelial Cell Regulators. J Immunol. 2018;201:215-229 pubmed publisher
  • flow cytometry; mouse; loading ...; fig s2a
Kwak J, Laskowski J, Li H, McSharry M, Sippel T, Bullock B, et al. Complement Activation via a C3a Receptor Pathway Alters CD4+ T Lymphocytes and Mediates Lung Cancer Progression. Cancer Res. 2018;78:143-156 pubmed publisher
  • flow cytometry; mouse; fig 1, 2, 3
Böiers C, Buza Vidas N, Jensen C, Pronk C, Kharazi S, Wittmann L, et al. Expression and role of FLT3 in regulation of the earliest stage of normal granulocyte-monocyte progenitor development. Blood. 2010;115:5061-8 pubmed publisher
Jia J, Zheng L, Ye L, Chen J, Shu S, Xu S, et al. CD11c+ microglia promote white matter repair after ischemic stroke. Cell Death Dis. 2023;14:156 pubmed publisher
Xiao P, Takiishi T, Violato N, Licata G, Dotta F, Sebastiani G, et al. NF-κB-inducing kinase (NIK) is activated in pancreatic β-cells but does not contribute to the development of diabetes. Cell Death Dis. 2022;13:476 pubmed publisher
Chiang H, Lu H, Sudhakar J, Chen Y, Shih N, Weng Y, et al. IL-22 initiates an IL-18-dependent epithelial response circuit to enforce intestinal host defence. Nat Commun. 2022;13:874 pubmed publisher
Jhan M, Chen C, Shen T, Tseng P, Wang Y, Satria R, et al. Polarization of Type 1 Macrophages Is Associated with the Severity of Viral Encephalitis Caused by Japanese Encephalitis Virus and Dengue Virus. Cells. 2021;10: pubmed publisher
Symeonidou V, Jakobczyk H, Bashanfer S, Malouf C, Fotopoulou F, Kotecha R, et al. Defining the fetal origin of MLL-AF4 infant leukemia highlights specific fatty acid requirements. Cell Rep. 2021;37:109900 pubmed publisher
Li Y, Shen S, Shao T, Jin M, Fan D, Lin A, et al. Mesenchymal stem cells attenuate liver fibrosis by targeting Ly6Chi/lo macrophages through activating the cytokine-paracrine and apoptotic pathways. Cell Death Discov. 2021;7:239 pubmed publisher
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Agarwal P, Isringhausen S, Li H, Paterson A, He J, Gomariz Á, et al. Mesenchymal Niche-Specific Expression of Cxcl12 Controls Quiescence of Treatment-Resistant Leukemia Stem Cells. Cell Stem Cell. 2019;24:769-784.e6 pubmed publisher
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product information
Product Type :
Antibody
Product Name :
CD45 Monoclonal Antibody (30-F11), Alexa Fluor 700, eBioscience™
Catalog # :
56-0451-80
Quantity :
25 µg
Price :
US 130
Clonality :
Monoclonal
Purity :
Affinity chromatography
Host :
Rat
Reactivity :
Mouse
Applications :
Flow Cytometry: 0.5 µg/test
Species :
Mouse
Clone :
30-F11
Isotype :
IgG2b, kappa
Storage :
4° C, store in dark, DO NOT FREEZE!
Description :
CD45 (LCA, leukocyte common antigen) is a receptor-type protein tyrosine phosphatase (PTP) ubiquitously expressed in all nucleated hematopoietic cells, comprising approximately 10% of all surface proteins in lymphocytes. CD45 is absent on non-hematopoietic cell lines, normal and malignant, non-hematopoietic tissues. CD45 glycoprotein is crucial in lymphocyte development and antigen signaling, serving as an important regulator of Src-family kinases. CD45 protein exists as multiple isoforms as a result of alternative splicing, differ in their extracellular domains but share identical transmembrane and cytoplasmic domains. CD45RA is an isoform of the CD45 complex and has restricted expression between different subtypes of lymphoid cells. CD45 isoforms differ in their ability to translocate into the glycosphingolipid-enriched membrane domains and their expression depends on cell type and physiological state of the cell. CD45 has been shown to be an essential regulator of T- and B-cell antigen receptor signaling and suppresses JAK kinases to regulate cytokine receptor signaling. CD45 is also important in promoting cell survival by modulating integrin-mediated signal transduction pathway, DNA fragmentation during apoptosis and inhibition or upregulation of various immunological functions.
Format :
Liquid
Applications w/Dilutions :
Flow Cytometry: 0.5 µg/test
Aliases :
B220; Cd45; CD45 antigen; CD45 antigen isoform 1 precursor; CD45 antigen isoform 2 precursor; CD45 antigen isoform 3 precursor; CD45 antigen isoform 4 precursor; CD45 antigen isoform 5 precursor; CD45 antigen isoform 6 precursor; CD45R; GP180; LCA; L-CA; leucocyte common antigen; leukocyte common antigen; leukocyte common antigen A; leukocyte common antigen B; leukocyte common antigen, CD45; loc; LOW QUALITY PROTEIN: receptor-type tyrosine-protein phosphatase C; LY5; Ly-5; Lymphocyte antigen 5; lymphocyte common antigen; Lyt-4; membrane tyrosine phosphatase; protein tyrosine phosphatase receptor type C; protein tyrosine phosphatase, receptor type C; protein tyrosine phosphatase, receptor type, C; protein tyrosine phosphatase, receptor type, c polypeptide; Protein tyrosine phosphatase, receptor-type, c polypeptide; protein tyrosine phosphatase; alternatively spliced; PTPRC; receptor-type tyrosine-protein phosphatase C; RT7; T200; T200 glycoprotein; T200 leukocyte common antigen; T220 and B220
more info or order :
company information
Invitrogen
Thermo Fisher Scientific
81 Wyman Street
Waltham, MA USA 02451
https://www.thermofisher.com
800-678-5599
headquarters: USA