Invitrogen Oct4 antibody

OCT3/4 Monoclonal Antibody (EM92), eBioscience
Invitrogen
catalog: 14-5841-80

rat monoclonal (EM92)
reactivity: human, mouse
application: western blot, immunohistochemistry, flow cytometry
citations: 9

quantity: 25 ug
price: US 177.00
to the supplier

OCT3/4 Monoclonal Antibody (EM92), eBioscience
Invitrogen
catalog: 14-5841-82

rat monoclonal (EM92)
reactivity: human, mouse
application: western blot, immunohistochemistry, immunocytochemistry, flow cytometry
citations: 10

quantity: 100 ug
price: US 447.00
to the supplier

OCT3/4 Monoclonal Antibody (EM92), eFluor 660, eBioscience
Invitrogen
catalog: 50-5841-80

rat monoclonal (EM92)
reactivity: human, mouse
conjugate: efluor 660
application: flow cytometry
citations: 11

quantity: 25 ug
price: US 177.00
to the supplier

OCT3/4 Monoclonal Antibody (EM92), eFluor 660, eBioscience
Invitrogen
catalog: 50-5841-82

rat monoclonal (EM92)
reactivity: human, mouse
conjugate: efluor 660
application: flow cytometry
citations: 12

quantity: 100 ug
price: US 451.00
to the supplier

OCT4 Polyclonal Antibody
Invitrogen
catalog: PA5-27438

domestic rabbit polyclonal
reactivity: human, mouse
application: western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section
citations: 15

Immunohistochemical analysis of paraffin-embedded Cal27 xenograft using OCT3/4 (PA5-27438) antibody at 1:500 dilution.

Flow cytometry on human embryonic stem cells staining with OCT3/4 (PA5-27438)antibody at 1:100 dilution(purple) or rabbit IgG (black).

Western Blot analysis of OCT3/4 using anti-OCT3/4 Polyclonal Antibody (PA5-27438), in Human ESC Cell (20 ug of cell lysate) and at a dilution of 1:10000.
quantity: 100 uL
price: US 474.00
to the supplier

OCT4 Monoclonal Antibody (9B7)
Invitrogen
catalog: MA1-104

mouse monoclonal (9B7)
reactivity: human, mouse
application: western blot, immunocytochemistry, flow cytometry
citations: 13

Flow cytometry analysis of Oct4 (red histogram) on human NCCIT cells. Cells were harvested, fixed with 4% formaldehyde, washed with PBS, and incubated with an Oct4 monoclonal antibody (Product # MA1-104) at a 1:100 dilution or PBS alone (blue histogram) for 1 hour on ice. For flow analysis, a 30-minute incubation with DyLight 488 goat anti-mouse IgG secondary antibody (Product # 35502) was performed and 10,000 cells were acquired for each sample.

Western blot analysis of Oct4 was performed by loading 75 ug of the indicated whole cell lysates and 5ul of the Lane Marker Reducing Sample Buffer (Product # 39000) per well onto a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked with 5% BSA/TBST (Product # 37525) for at least 1 hour. The membrane was probed with an Oct4 monoclonal antibody (Product # MA1-104) at a dilution of 1:1000 overnight at 4°C on a rocking platform, washed in TBS-0.1%Tween 20, and probed with a goat anti-mouse IgG-HRP secondary antibody (Product # 32430) at a dilution of 1:20,000 for at least 1 hour. Chemiluminescent detection was performed using SuperSignal West Dura (Product # 34075). **Note: the antibody is detecting Oct4 in the human embryonal carcinoma cells, but not in negative control HeLa cells. Unknown bands at 80kDa were also observed with this antibody.

Immunofluorescent analysis of Oct4 (green) in retinoic acid-treated NCCIT cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 0.3% BSA/TBST (Product # 37525) for 15 minutes at room temperature. Cells were probed with an Oct4 monoclonal antibody (Product # MA1-104) at a dilution of 1:100 for at least 1 hour at room temperature, washed with PBS, and incubated with DyLight 488 goat anti-mouse IgG secondary antibody (Product # 35502) at a dilution of 1:400 for 30 minutes at room temperature. Nuclei (blue) were stained with Hoechst 33342 dye (Product # 62249). Images were taken on a Thermo Scientific ArrayScan at 20X magnification. **Note: the absence of Oct4 staining in a subset of the NCCIT cells suggests differentiation. In addition to the nuclear staining of Oct4, the antibody also shows some cytoplasmic staining.
quantity: 100 ug
price: US 409.00
to the supplier

OCT3/4 Monoclonal Antibody (EM92), PE, eBioscience
Invitrogen
catalog: 12-5841-80

rat monoclonal (EM92)
reactivity: human, mouse
conjugate: PE
application: flow cytometry
citations: 5

quantity: 25 ug
price: US 198.00
to the supplier

OCT3/4 Monoclonal Antibody (EM92), PE, eBioscience
Invitrogen
catalog: 12-5841-82

rat monoclonal (EM92)
reactivity: human, mouse
conjugate: PE
application: flow cytometry
citations: 5

quantity: 100 ug
price: US 427.00
to the supplier

OCT3/4 Monoclonal Antibody (EM92), Alexa Fluor 488, eBioscience
Invitrogen
catalog: 53-5841-82

rat monoclonal (EM92)
reactivity: human, mouse
conjugate: AF488
application: immunocytochemistry, flow cytometry
citations: 7

quantity: 100 ug
price: US 454.00
to the supplier

OCT4 Monoclonal Antibody (T.631.9)
Invitrogen
catalog: MA5-14845

domestic rabbit monoclonal (T.631.9)
reactivity: human, mouse
application: western blot, immunocytochemistry, flow cytometry
citations: 7

quantity: 100 uL
price: US 464.00
to the supplier

OCT4 Recombinant Rabbit Monoclonal Antibody (3H8L6)
Invitrogen
catalog: 701756

domestic rabbit recombinant (3H8L6)
reactivity: human, mouse
application: western blot, immunocytochemistry, ChIP-Seq
citations: 7

quantity: 100 ug
price: US 520.00
to the supplier

OCT3/4 Monoclonal Antibody (EM92), eBioscience
Invitrogen
catalog: 14-5841-37

rat monoclonal (EM92)
reactivity: human, mouse
application: western blot, immunohistochemistry
citations: 6

quantity: 2 mg
price: US 1482.00
to the supplier

OCT4 Polyclonal Antibody
Invitrogen
catalog: PA1-16943

domestic rabbit polyclonal
reactivity: human, mouse, rat, bovine
application: western blot, immunohistochemistry, immunocytochemistry, flow cytometry, immunohistochemistry - paraffin section
citations: 3

FACS staining of NTERA-2 cells using PA1-16943 at a 1:50 dilution detected using Dylight-488 conjugated goat anti-rabbit secondary antibody.

OCT4 staining in NTERA-2 cells detected with a Dylight 488 labeled secondary antibody.

Western Blot detection of OCT4 in mouse brain lysate using PA1-16943.
quantity: 100 uL
price: US 514.00
to the supplier

OCT3/4 Monoclonal Antibody (EM92), eFluor 570, eBioscience
Invitrogen
catalog: 41-5841-80

rat monoclonal (EM92)
reactivity: human, mouse
conjugate: eFluor 570
application: immunocytochemistry
citations: 2

quantity: 25 ug
price: US 187.00
to the supplier

OCT3/4 Polyclonal Antibody
Invitrogen
catalog: PA5-96860

domestic rabbit polyclonal
reactivity: human, mouse
application: western blot, ELISA, immunohistochemistry, immunohistochemistry - paraffin section
citations: 2

quantity: 100 uL
price: US 464.00
to the supplier

OCT4 Monoclonal Antibody (5F7)
Invitrogen
catalog: MA5-15736

mouse monoclonal (5F7)
reactivity: human
application: western blot, ELISA
citations: 1

quantity: 100 uL
price: US 504.00
to the supplier

OCT4 Polyclonal Antibody
Invitrogen
catalog: PA5-20887

domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, immunohistochemistry
citations: 1

Immunohistochemical staining of rat liver tissue using PA5-20887 anti-POU5F1 antibody at a concentration of 5 µg/ml.
quantity: 100 ug
price: US 464.00
to the supplier

OCT4 Monoclonal Antibody (9B7), DyLight 488
Invitrogen
catalog: MA1-104-D488

mouse monoclonal (9B7)
reactivity: human
conjugate: DyLight488
application: immunocytochemistry
citations: 1

quantity: 100 uL
price: US 409.00
to the supplier

OCT4 Monoclonal Antibody (GT486)
Invitrogen
catalog: MA5-31458

mouse monoclonal (GT486)
reactivity: human, mouse
application: western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section
citations: 1

quantity: 100 uL
price: US 474.00
to the supplier

OCT4 Polyclonal Antibody
Invitrogen
catalog: PA5-18403

domestic goat polyclonal
reactivity: human, mouse
application: western blot

Western Blot staining of Human Liver lysate using PA5-18403 at a concentration of 1.0 ug/ml, the primary antibody incubation was 1 hour and the detection method was chemiluminescence.
quantity: 100 ug
price: US 464.00
to the supplier

OCT4 Polyclonal Antibody
Invitrogen
catalog: PA5-19307

domestic goat polyclonal
reactivity: human, mouse
application: western blot

Western Blot staining of NIH/3T3 cell lysate using PA5-19307 at a concentration of 1.0 ug/ml, the primary antibody incubation was 1 hour and the detection method was chemiluminescence.
quantity: 100 ug
price: US 474.00
to the supplier

OCT4 Polyclonal Antibody, DyLight 650
Invitrogen
catalog: PA5-22734

domestic rabbit polyclonal
reactivity: human, mouse, rat, bovine
conjugate: DyLight 650
application: western blot, immunohistochemistry, immunocytochemistry, flow cytometry

quantity: 100 uL
price: US 454.00
to the supplier

OCT4 Polyclonal Antibody, DyLight 488
Invitrogen
catalog: PA5-22735

domestic rabbit polyclonal
reactivity: human, mouse, rat, bovine
conjugate: DyLight488
application: western blot, immunohistochemistry, immunocytochemistry, flow cytometry

quantity: 100 uL
price: US 474.00
to the supplier

OCT4 Polyclonal Antibody
Invitrogen
catalog: PA5-27194

domestic rabbit polyclonal
reactivity: human
application: western blot, immunocytochemistry, immunoprecipitation, flow cytometry

Flow cytometry on human embryonic stem cells staining with OCT3/4 (PA5-27194)antibody at 1:100 dilution(purple) or rabbit IgG (black).

Immunofluorescence analysis of paraformaldehyde-fixed Human ESC cells using OCT3/4 (PA5-27194) antibody at 1:80 dilution.

Western Blot analysis of OCT3/4 using anti-OCT3/4 Polyclonal Antibody (PA5-27194), in NT2D1 Cell Lysate (30 ug of cell lysate) and at a dilution of 1:500.
quantity: 100 uL
price: US 464.00
to the supplier

OCT4 Polyclonal Antibody, DyLight 550
Invitrogen
catalog: PA5-22736

domestic rabbit polyclonal
reactivity: human, mouse, rat, bovine
conjugate: DyLight 550
application: western blot, immunohistochemistry, immunocytochemistry, flow cytometry

quantity: 100 uL
price: US 474.00
to the supplier

OCT4 Recombinant Superclonal Antibody (3HCLC)
Invitrogen
catalog: 710788

domestic rabbit monoclonal (3HCLC)
reactivity: human
application: western blot, immunocytochemistry

quantity: 100 ug
price: US 520.00
to the supplier

OCT4 Monoclonal Antibody (GT735)
Invitrogen
catalog: MA5-31460

mouse monoclonal (GT735)
reactivity: human
application: western blot, immunocytochemistry, immunoprecipitation, flow cytometry

quantity: 100 uL
price: US 474.00
to the supplier

OCT4 Polyclonal Antibody
Invitrogen
catalog: PA5-85098

domestic rabbit polyclonal
reactivity: human, mouse
application: western blot, immunocytochemistry, immunoprecipitation, flow cytometry

quantity: 100 uL
price: US 454.00
to the supplier

OCT3/4 Polyclonal Antibody
Invitrogen
catalog: PA5-86191

domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

quantity: 100 uL
price: US 464.00
to the supplier

OCT4 Recombinant Rabbit Monoclonal Antibody (3H8L1.12)
Invitrogen
catalog: 703927

domestic rabbit recombinant (3H8L1.12)
reactivity: human
application: western blot, immunocytochemistry

quantity: 100 ug
price: US 606.00
to the supplier

OCT4 Recombinant Rabbit Monoclonal Antibody (3H8L6)
Invitrogen
catalog: 740020R

domestic rabbit recombinant (3H8L6)
reactivity: human, mouse
application: western blot, immunocytochemistry, flow cytometry

quantity: 200 uL
price: US 522.00
to the supplier

OCT4 Recombinant Rabbit Monoclonal Antibody (3H8L6)
Invitrogen
catalog: 740020R20UG

domestic rabbit recombinant (3H8L6)
reactivity: human, mouse
application: western blot, immunocytochemistry, flow cytometry

quantity: 40 uL
price: US 208.00
to the supplier

OCT4 Recombinant Rabbit Monoclonal Antibody (SD0750)
Invitrogen
catalog: MA5-41181

domestic rabbit recombinant (SD0750)
reactivity: human, mouse
application: western blot, immunohistochemistry, immunocytochemistry, flow cytometry, immunohistochemistry - paraffin section

quantity: 100 uL
price: US 470.00
to the supplier

OCT4 Polyclonal Antibody
Invitrogen
catalog: PA5-141018

domestic rabbit polyclonal
reactivity: human, mouse
application: western blot

quantity: 100 uL
price: US 460.00
to the supplier

OCT4 Recombinant Rabbit Monoclonal Antibody (3H8L6), Alexa Fluor Plus 488
Invitrogen
catalog: 701756RP488