mouse monoclonal (TRA-1-60)
reactivity: human, rhesus macaque
application: western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry
citations: 67
quantity: 100 ug
price: US 314.00
to the supplier
mouse monoclonal (TRA-1-60)
reactivity: human
conjugate: PE
application: flow cytometry
citations: 32
quantity: 100 ug
price: US 265.00
to the supplier
mouse monoclonal (TRA-1-60)
reactivity: human
conjugate: biotin
application: immunocytochemistry, flow cytometry
citations: 36
quantity: 100 ug
price: US 185.00
to the supplier
mouse monoclonal (TRA-1-81)
reactivity: human, rhesus macaque
application: western blot, immunocytochemistry, immunoprecipitation, flow cytometry
citations: 43
quantity: 100 ug
price: US 318.00
to the supplier
TRA-1-60 Monoclonal Antibody (TRA-1-60)
Invitrogen
catalog: MA1-023
mouse monoclonal (TRA-1-60)
reactivity: human, mouse
application: western blot, immunohistochemistry, immunocytochemistry, flow cytometry
citations: 42

Immunofluorescent analysis of TRA-1-60 using anti-TRA-1-60 monoclonal antibody (Product# MA1-023) shows staining on the cell surface of human HEL 11.4 iPS cells, indicating pluripotency. TRA-1-60 staining (green) and an overlay image of TRA-1-60 with DAPI (blue) is shown. HEL 11.4 iPS cells were grown on matrigel coated chamber slides and fixed with formaldehyde prior to staining. Cells were probed with a Monoclonal antibody recognizing TRA-1-60 (Product# MA1-023) at a dilution of 1:50 over night at 4 ºC, washed with PBS and incubated with a fluorescein conjugated secondary antibody at a dilution of 1:100 for 60 minutes at room temperature. Images were taken at 20X magnification.

Flow cytometry analysis of TRA-1-60 using anti-TRA-1-60 monoclonal antibody (Product# MA1-023) shows positive staining of H9 human embryonic stem cells (blue line). H9 human embryonic stem cells were harvested, fixed and washed with PBS. Cells were incubated with anti-TRA-1-60 monoclonal antibody (Product# MA1-023) or control at a 1:100 dilution for 1 hour on ice, followed by 30 min incubation with fluorescein conjugated secondary antibody. 100,000 cells were stained for each sample.

Western blot analysis of TRA-1-60 was performed by loading 100 µg of NTERA-2 whole cell lysate onto a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked with 5% BSA/TBST for at least 1 hour. Membranes were probed with a mouse monoclonal antibody recognizing TRA-1-60 (Product# MA1-023) at a dilution of 1:500 overnight at 4°C on a rocking platform. Membranes were washed in TBS-0.1%Tween 20 and probed with a goat anti-mouse-IgM HRP secondary antibody at a dilution of 1:20,000 for at least one hour. Membranes were washed and chemiluminescent detection performed using Pierce Super Signal West Dura (Product #34075).
quantity: 100 ug
price: US 389.00
to the supplier
TRA-1-60 Monoclonal Antibody (cl.A)
Invitrogen
catalog: 41-1000
mouse monoclonal (cl.A)
reactivity: human
application: western blot, immunocytochemistry, flow cytometry, immunohistochemistry - paraffin section
citations: 38
quantity: 100 ug
price: US 471.00
to the supplier
mouse monoclonal (TRA-1-81)
reactivity: human
conjugate: PE
application: flow cytometry
citations: 25
quantity: 100 ug
price: US 299.00
to the supplier
mouse monoclonal (TRA-1-81)
reactivity: human
conjugate: APC
application: flow cytometry
citations: 21
quantity: 100 Tests
price: US 394.00
to the supplier
mouse monoclonal (TRA-1-60)
reactivity: human
conjugate: Percp/efluor 710
application: flow cytometry
citations: 21
quantity: 100 ug
price: US 466.00
to the supplier
PODXL Monoclonal Antibody (3D3)
Invitrogen
catalog: 39-3800
mouse monoclonal (3D3)
reactivity: human, dogs
application: western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunohistochemistry - frozen section
citations: 19
quantity: 100 ug
price: US 520.00
to the supplier
TRA-1-81 Monoclonal Antibody (cl.26)
Invitrogen
catalog: 41-1100
mouse monoclonal (cl.26)
reactivity: human
application: western blot, ELISA, immunocytochemistry, immunoprecipitation, flow cytometry
citations: 16
quantity: 100 ug
price: US 451.00
to the supplier
TRA-1-81 Monoclonal Antibody (TRA-1-81)
Invitrogen
catalog: MA1-024
mouse monoclonal (TRA-1-81)
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, flow cytometry
citations: 14

Immunofluorescent analysis of TRA-1-81 using anti-TRA-1-81 monoclonal antibody (Product# MA1-024) shows staining on the cell surface of human HEL 11.4 iPS cells, indicating pluripotency. TRA-1-81 staining (green) and an overlay image of TRA-1-81 with DAPI (blue) is shown. HEL 11.4 iPS cells were grown on matrigel coated chamber slides and fixed with formaldehyde prior to staining. Cells were probed with a Monoclonal antibody recognizing TRA-1-81 (Product# MA1-024) at a dilution of 1:20 overnight at 4 ºC, washed with PBS and incubated with a fluorescein conjugated secondary antibody at a dilution of 1:100 for 60 minutes at room temperature. Images were taken at 20X magnification.

Flow cytometry analysis of TRA-1-81 using anti-TRA-1-81 monoclonal antibody (Product# MA1-024) shows positive staining of HEL 11.4 iPS cells (blue line). HEL 11.4 iPS cells were harvested, fixed and washed with PBS. Cells were incubated with anti-TRA-1-81 monoclonal antibody (Product# MA1-024) or control at a 1:100 dilution for 1 hour on ice, followed by 30 min incubation with fluorescein conjugated secondary antibody. 100,000 cells were stained for each sample.

Western blot analysis of TRA-1-81 was performed by loading 100ug of NTERA-2 whole cell lysate onto a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked with 5% BSA/TBST for at least 1 hour. Membranes were probed with a mouse monoclonal antibody recognizing TRA-1-81 (Product # MA1-024) at a dilution of 1:500 overnight at 4°C on a rocking platform. Membranes were washed in TBS-0.1%Tween 20 and probed with a goat anti-mouse-IgM HRP secondary antibody at a dilution of 1:20,000 for at least one hour. Membranes were washed and chemiluminescent detection performed using Super Signal West Dura (Product #34075).
quantity: 100 ug
price: US 389.00
to the supplier
mouse monoclonal (B34D1.3)
reactivity: human
conjugate: PE
application: flow cytometry
citations: 4
quantity: 100 Tests
price: US 318.00
to the supplier
PODXL Polyclonal Antibody
Invitrogen
catalog: PA1-46169
domestic rabbit polyclonal
reactivity: human, mouse
application: western blot, immunocytochemistry
citations: 1
PODXL Monoclonal Antibody (5D4)
Invitrogen
catalog: MA5-15845
mouse monoclonal (5D4)
reactivity: human
application: western blot, ELISA

quantity: 100 uL
price: US 491.00
to the supplier
PODXL Monoclonal Antibody (5F5)
Invitrogen
catalog: MA5-15846
mouse monoclonal (5F5)
reactivity: human
application: western blot, ELISA, immunohistochemistry, flow cytometry, immunohistochemistry - paraffin section
PODXL Monoclonal Antibody
Invitrogen
catalog: 433140
mouse monoclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry
quantity: 100 ug
price: US 520.00
to the supplier
PODXL Polyclonal Antibody
Invitrogen
catalog: PA5-80915
domestic rabbit polyclonal
reactivity: human
application: immunohistochemistry, immunohistochemistry - paraffin section
quantity: 100 uL
price: US 319.00
to the supplier
PODXL Polyclonal Antibody
Invitrogen
catalog: PA5-82001
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section
quantity: 100 uL
price: US 514.00
to the supplier
PODXL Polyclonal Antibody
Invitrogen
catalog: PA5-83778
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section
quantity: 100 uL
price: US 522.00
to the supplier
domestic rabbit recombinant (6S9A9)
reactivity: human
application: western blot, ELISA, immunohistochemistry, immunohistochemistry - paraffin section
quantity: 100 uL
price: US 470.00
to the supplier
domestic rabbit recombinant (HL2047)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section
quantity: 100 uL
price: US 460.00
to the supplier