product summary
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company name :
Invitrogen
other brands :
NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience
product type :
antibody
product name :
Fibronectin Monoclonal Antibody (FN-3), Alexa Fluor™ 488, eBioscience
catalog :
53-9869-82
quantity :
100 µg
price :
US 450.00
clonality :
monoclonal
host :
mouse
conjugate :
AF488
clone name :
Fn-3
reactivity :
human
application :
immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section
more info or order :
Invitrogen product webpage
citations: 11
Published Application/Species/Sample/DilutionReference
  • immunocytochemistry; human; 1:400; fig s9a
Zehender A, Li Y, Lin N, Stefanica A, Nüchel J, Chen C, et al. TGFβ promotes fibrosis by MYST1-dependent epigenetic regulation of autophagy. Nat Commun. 2021;12:4404 pubmed publisher
  • immunocytochemistry; human; loading ...; fig 1
Bon H, Hales P, Lumb S, Holdsworth G, Johnson T, Qureshi O, et al. Spontaneous Extracellular Matrix Accumulation in a Human in vitro Model of Renal Fibrosis Is Mediated by αV Integrins. Nephron. 2019;:1-23 pubmed publisher
Betriu N, Andreeva A, Alonso A, Semino C. Increased Stiffness Downregulates Focal Adhesion Kinase Expression in Pancreatic Cancer Cells Cultured in 3D Self-Assembling Peptide Scaffolds. Biomedicines. 2022;10: pubmed publisher
Maamra M, Benayad O, Matthews D, Kettleborough C, Atkinson J, Cain K, et al. Transglutaminase 2: Development of therapeutic antibodies reveals four inhibitory epitopes and confirms extracellular function in fibrotic remodelling. Br J Pharmacol. 2022;179:2697-2712 pubmed publisher
Gao H, Tian Q, Zhu L, Feng J, Zhou Y, Yang J. 3D Extracellular Matrix Regulates the Activity of T Cells and Cancer Associated Fibroblasts in Breast Cancer. Front Oncol. 2021;11:764204 pubmed publisher
Marwick J, Elliott R, Longden J, Makda A, Hirani N, Dhaliwal K, et al. Application of a High-Content Screening Assay Utilizing Primary Human Lung Fibroblasts to Identify Antifibrotic Drugs for Rapid Repurposing in COVID-19 Patients. SLAS Discov. 2021;26:1091-1106 pubmed publisher
Hill L, Botfield H, Begum G, Qureshi O, Vigneswara V, Masood I, et al. ILB® resolves inflammatory scarring and promotes functional tissue repair. NPJ Regen Med. 2021;6:3 pubmed publisher
Good R, Eley J, Gower E, Butt G, Blanchard A, Fisher A, et al. A high content, phenotypic 'scar-in-a-jar' assay for rapid quantification of collagen fibrillogenesis using disease-derived pulmonary fibroblasts. BMC Biomed Eng. 2019;1:14 pubmed publisher
Tomasini Johansson B, Zbyszynski P, Toraason I, Peters D, Kwon G. PEGylated pUR4/FUD peptide inhibitor of fibronectin fibrillogenesis decreases fibrosis in murine Unilateral Ureteral Obstruction model of kidney disease. PLoS ONE. 2018;13:e0205360 pubmed publisher
Qureshi O, Bon H, Twomey B, Holdsworth G, Ford K, Bergin M, et al. An immunofluorescence assay for extracellular matrix components highlights the role of epithelial cells in producing a stable, fibrillar extracellular matrix. Biol Open. 2017;6:1423-1433 pubmed publisher
Jiang X, Seo Y, Chang J, Coveler A, Nigjeh E, Pan S, et al. Long-lived pancreatic ductal adenocarcinoma slice cultures enable precise study of the immune microenvironment. Oncoimmunology. 2017;6:e1333210 pubmed publisher
product information
Product Type :
Antibody
Product Name :
Fibronectin Monoclonal Antibody (FN-3), Alexa Fluor™ 488, eBioscience
Catalog # :
53-9869-82
Quantity :
100 µg
Price :
US 450.00
Clonality :
Monoclonal
Purity :
Affinity chromatography
Host :
Mouse
Reactivity :
Human
Applications :
Immunocytochemistry: Assay-Dependent, Immunohistochemistry (Paraffin): 10 µg/mL
Species :
Human
Clone :
FN-3
Isotype :
IgG1
Storage :
4° C, store in dark, DO NOT FREEZE!
Description :
Fibronectin is a disulfide-bonded dimer with a molecular weight range of 230-250 kDa. In the extracellular matrix of several connective tissues and vessels, fibronectin is present as an insoluble protein that is extensively cross-linked by interchain disulfide bonds forming high molecular mass polymers. Fibronectin is most abundant during embryonic development and tissue remodeling. Fibronectin is also present at high concentrations as a soluble plasma protein. Fibronectin is organized as a linear series of repeating modules which form domains for interaction with fibronectin itself, other matrix components (e.g. collagen and heparin) and receptors on cells (e.g. integrins). Fibronectin is present in a soluble dimeric form in plasma, and in a dimeric or multimeric form at the cell surface and in extracellular matrix. Fibronectin is involved in cell adhesion and migration processes including embryogenesis, wound healing, blood coagulation, host defense, and metastasis. Fibronectin has been implicated in carcinoma development in lung cancer. Further, Fibronectin expression is increased especially in non-small cell lung carcinoma. The adhesion of lung carcinoma cells to Fibronectin enhances tumorgenecity and confers resistance to apoptosis induced by standard chemotherapeutic agents. The gene encoding Fibronectin has three regions subject to alternative splicing, with the potential to produce 20 different transcript variants. However, the full-length nature of some Fibronectin variants has not been determined.
Format :
Liquid
Applications w/Dilutions :
Immunocytochemistry: Assay-Dependent, Immunohistochemistry (Paraffin): 10 µg/mL
Aliases :
Anastellin; CIG; Cold insoluble globulin (CIG); Cold-insoluble globulin; cumulus cell-specific fibronectin 1 transcript; DKFZp686F10164; DKFZp686H0342; DKFZp686I1370; DKFZp686O13149; E330027I09; ED-B; embryo-specific fibronectin 1 transcript; ferritin L subunit; ferritin L-chain; FIBNEC; fibronectin; fibronectin 1; fibronectin ED-A- region; fibronectin ED-B+ region; fibronectin variable region; FINC; FN; FN1; fn-1; FNZ; GFND; GFND2; LETS; LOW QUALITY PROTEIN: fibronectin; Migration stimulating factor (MSF); migration-stimulating factor; MSF; Ugl-Y1; Ugl-Y2; Ugl-Y3
more info or order :
Invitrogen product webpage
company information
Invitrogen
Thermo Fisher Scientific
81 Wyman Street
Waltham, MA USA 02451
https://www.thermofisher.com
800-678-5599
headquarters: USA