AIRE Monoclonal Antibody (5H12), Alexa Fluor™ 488, eBioscience | Invitrogen 53-5934-82 product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

AIRE Monoclonal Antibody (5H12), Alexa Fluor™ 488, eBioscience

catalog :

53-5934-82

quantity :

100 µg

price :

US 446

clonality :

monoclonal

host :

rat

conjugate :

AF488

clone name :

5H12

reactivity :

mouse

application :

immunohistochemistry, immunocytochemistry, flow cytometry, immunohistochemistry - frozen section

more info or order :

Invitrogen product webpage

citations: 25

Published Application/Species/Sample/Dilution	Reference
immunohistochemistry - frozen section; mouse; 1:200; loading ...; fig 3s2a flow cytometry; mouse; 1:200; loading ...; fig 3c	Lopes N, Boucherit N, Santamaria J, Provin N, Charaix J, Ferrier P, et al. Thymocytes trigger self-antigen-controlling pathways in immature medullary thymic epithelial stages. elife. 2022;11: pubmed publisher
	Le Voyer T, Parent A, Liu X, Cederholm A, Gervais A, Rosain J, et al. Autoantibodies against type I IFNs in humans with alternative NF-κB pathway deficiency. Nature. 2023;623:803-813 pubmed publisher
	LUCAS B, White A, Klein F, Veiga Villauriz C, Handel A, Bacon A, et al. Embryonic keratin19+ progenitors generate multiple functionally distinct progeny to maintain epithelial diversity in the adult thymus medulla. Nat Commun. 2023;14:2066 pubmed publisher
	Fujimori S, Ohigashi I, Abe H, Matsushita Y, Katagiri T, Taketo M, et al. Fine-tuning of β-catenin in mouse thymic epithelial cells is required for postnatal T-cell development. elife. 2022;11: pubmed publisher
	Teng F, Tach xf3 Pi xf1 ot R, Sung B, Farber D, Worgall S, Hammad H, et al. ILC3s control airway inflammation by limiting T cell responses to allergens and microbes. Cell Rep. 2021;37:110051 pubmed publisher
	Goldfarb Y, Givony T, Kadouri N, Dobe x161 J, Peligero Cruz C, Zalayat I, et al. Mechanistic dissection of dominant AIRE mutations in mouse models reveals AIRE autoregulation. J Exp Med. 2021;218: pubmed publisher
	Zhang Q, Liang Z, Zhang J, Lei T, Dong X, Su H, et al. Sirt6 Regulates the Development of Medullary Thymic Epithelial Cells and Contributes to the Establishment of Central Immune Tolerance. Front Cell Dev Biol. 2021;9:655552 pubmed publisher
	Farini A, Sitzia C, Villa C, Cassani B, Tripodi L, Legato M, et al. Defective dystrophic thymus determines degenerative changes in skeletal muscle. Nat Commun. 2021;12:2099 pubmed publisher
	Swann J, Nusser A, Morimoto R, Nagakubo D, Boehm T. Retracing the evolutionary emergence of thymopoiesis. Sci Adv. 2020;6: pubmed publisher
	Tsukasaki M, Asano T, Muro R, Huynh N, Komatsu N, Okamoto K, et al. OPG Production Matters Where It Happened. Cell Rep. 2020;32:108124 pubmed publisher
	Ohigashi I, Tanaka Y, Kondo K, Fujimori S, Kondo H, Palin A, et al. Trans-omics Impact of Thymoproteasome in Cortical Thymic Epithelial Cells. Cell Rep. 2019;29:2901-2916.e6 pubmed publisher
	Koh A, Miller E, Buenrostro J, Moskowitz D, Wang J, Greenleaf W, et al. Rapid chromatin repression by Aire provides precise control of immune tolerance. Nat Immunol. 2018;19:162-172 pubmed publisher
	Liang Z, Zhang L, Su H, Luan R, Na N, Sun L, et al. MTOR signaling is essential for the development of thymic epithelial cells and the induction of central immune tolerance. Autophagy. 2017;: pubmed publisher
	Zhu F, Willette Brown J, Song N, Lomada D, Song Y, Xue L, et al. Autoreactive T Cells and Chronic Fungal Infection Drive Esophageal Carcinogenesis. Cell Host Microbe. 2017;21:478-493.e7 pubmed publisher
	Takahashi T, Asano Y, Sugawara K, Yamashita T, Nakamura K, Saigusa R, et al. Epithelial Fli1 deficiency drives systemic autoimmunity and fibrosis: Possible roles in scleroderma. J Exp Med. 2017;214:1129-1151 pubmed publisher
	Bansal K, Yoshida H, Benoist C, Mathis D. The transcriptional regulator Aire binds to and activates super-enhancers. Nat Immunol. 2017;18:263-273 pubmed publisher
	Yanagihara T, Sanematsu F, Sato T, Uruno T, Duan X, Tomino T, et al. Intronic regulation of Aire expression by Jmjd6 for self-tolerance induction in the thymus. Nat Commun. 2015;6:8820 pubmed publisher
	McCarthy N, Cowan J, Nakamura K, Bacon A, Baik S, White A, et al. Osteoprotegerin-Mediated Homeostasis of Rank+ Thymic Epithelial Cells Does Not Limit Foxp3+ Regulatory T Cell Development. J Immunol. 2015;195:2675-82 pubmed publisher
	Coder B, Wang H, Ruan L, Su D. Thymic involution perturbs negative selection leading to autoreactive T cells that induce chronic inflammation. J Immunol. 2015;194:5825-37 pubmed publisher
	White A, Jenkinson W, Cowan J, Parnell S, Bacon A, Jones N, et al. An essential role for medullary thymic epithelial cells during the intrathymic development of invariant NKT cells. J Immunol. 2014;192:2659-66 pubmed publisher
	Waterfield M, Khan I, Cortez J, Fan U, Metzger T, Greer A, et al. The transcriptional regulator Aire coopts the repressive ATF7ip-MBD1 complex for the induction of immunotolerance. Nat Immunol. 2014;15:258-65 pubmed publisher
	Coquet J, Ribot J, Bąbała N, Middendorp S, van der Horst G, Xiao Y, et al. Epithelial and dendritic cells in the thymic medulla promote CD4+Foxp3+ regulatory T cell development via the CD27-CD70 pathway. J Exp Med. 2013;210:715-28 pubmed publisher
	Baik S, Jenkinson E, Lane P, Anderson G, Jenkinson W. Generation of both cortical and Aire(+) medullary thymic epithelial compartments from CD205(+) progenitors. Eur J Immunol. 2013;43:589-94 pubmed publisher
	Irla M, Guenot J, Sealy G, Reith W, Imhof B, Serge A. Three-dimensional visualization of the mouse thymus organization in health and immunodeficiency. J Immunol. 2013;190:586-96 pubmed publisher
	Rossi S, Kim M, Leibbrandt A, Parnell S, Jenkinson W, Glanville S, et al. RANK signals from CD4(+)3(-) inducer cells regulate development of Aire-expressing epithelial cells in the thymic medulla. J Exp Med. 2007;204:1267-72 pubmed

product information

Product Type :

Antibody

Product Name :

AIRE Monoclonal Antibody (5H12), Alexa Fluor™ 488, eBioscience

Catalog # :

53-5934-82

Quantity :

100 µg

Price :

US 446

Clonality :

Monoclonal

Purity :

Affinity chromatography

Host :

Rat

Reactivity :

Mouse

Applications :

Flow Cytometry: Assay-Dependent, Immunocytochemistry: 10 µg/mL, Immunohistochemistry (Frozen): 10 µg/mL

Species :

Mouse

Clone :

5H12

Isotype :

IgG2c, kappa

Storage :

4° C, store in dark, DO NOT FREEZE!

Description :

AIRE is a transcriptional regulator protein that binds to DNA as a dimer and tetramer, but not as a monomer, and may be involved in the regulation of gene expression. A nuclear protein, AIRE is a 545 amino acid protein with an HSR domain, two PHD type zinc fingers, and a SAND domain, and may be present in soluble high-molecular-weight complexes. It may form homodimer, homotetramer and also nuclear bodies and interacts with the transcriptional coactivator CBP and CREBBP. Three isoforms of AIRE are reported and one of them is predicted to be a candidate for nuclear-mediated decay (NMD). AIRE is involved in immune regulation and is known to mediate E3 ubiquitin ligase activity. Defects in AIRE are responsible for the rare autosomal-recessive systemic autoimmune disease termed autoimmune polyendocrinopathy-candidiasis-ectodermal dystrophy (APECED).

Format :

Liquid

Applications w/Dilutions :

Flow Cytometry: Assay-Dependent, Immunocytochemistry: 10 µg/mL, Immunohistochemistry (Frozen): 10 µg/mL

Aliases :

Aire; AIRE protein; AIRE1; APECED; APECED protein; APECED protein homolog; APS1; APSI; Autoimmune polyendocrinopathy candidiasis ectodermal dystrophy protein; autoimmune polyendocrinopathy candidiasis ectodermal dystrophy protein homolog; Autoimmune regulator; autoimmune regulator (autoimmune polyendocrinopathy candidiasis ectodermal dystrophy); autoimmune regulator AIRE1a; LOW QUALITY PROTEIN: autoimmune regulator; OTTHUMP00000109529; PGA1

more info or order :

Invitrogen product webpage