iNOS Monoclonal Antibody (CXNFT), Alexa Fluor™ 488, eBioscience | Invitrogen 53-5920-82 product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

iNOS Monoclonal Antibody (CXNFT), Alexa Fluor™ 488, eBioscience

catalog :

53-5920-82

quantity :

100 µg

price :

US 383.00

clonality :

monoclonal

host :

rat

conjugate :

AF488

clone name :

CXNFT

reactivity :

mouse

application :

western blot, immunocytochemistry, flow cytometry

more info or order :

Invitrogen product webpage

citations: 20

Published Application/Species/Sample/Dilution	Reference
flow cytometry; mouse; 1:300; loading ...; fig 3a	Glausen T, Carrillo G, Jin R, Boyle J, Saeij J, Wohlfert E, et al. The Toxoplasma Polymorphic Effector GRA15 Mediates Seizure Induction by Modulating Interleukin-1 Signaling in the Brain. MBio. 2021;12:e0133121 pubmed publisher
	Zha Y, Liu H, Lin X, Yu L, Gao P, Li Y, et al. Immune Deviation in the Decidua During Term and Preterm Labor. Front Immunol. 2022;13:877314 pubmed publisher
	Li G, Cui L, Wang G. miR-155-5p regulates macrophage M1 polarization and apoptosis in the synovial fluid of patients with knee osteoarthritis. Exp Ther Med. 2021;21:68 pubmed publisher
	Kapralov A, Yang Q, Dar H, Tyurina Y, Anthonymuthu T, Kim R, et al. Redox lipid reprogramming commands susceptibility of macrophages and microglia to ferroptotic death. Nat Chem Biol. 2020;16:278-290 pubmed publisher
	Li M, Guo X, Qi W, Wu Z, de Bruijn J, Xiao Y, et al. Macrophage polarization plays roles in bone formation instructed by calcium phosphate ceramics. J Mater Chem B. 2020;8:1863-1877 pubmed publisher
	Wu R, Liu C, Deng X, Chen L, Hao S, Ma L. Enhanced alleviation of aGVHD by TGF-β1-modified mesenchymal stem cells in mice through shifting MΦ into M2 phenotype and promoting the differentiation of Treg cells. J Cell Mol Med. 2020;24:1684-1699 pubmed publisher
	Yi S, Zhai J, Niu R, Zhu G, Wang M, Liu J, et al. Eosinophil recruitment is dynamically regulated by interplay among lung dendritic cell subsets after allergen challenge. Nat Commun. 2018;9:3879 pubmed publisher
	Chen D, Xie J, Fiskesund R, Dong W, Liang X, Lv J, et al. Chloroquine modulates antitumor immune response by resetting tumor-associated macrophages toward M1 phenotype. Nat Commun. 2018;9:873 pubmed publisher
	Lee S, Charmoy M, Romano A, Paun A, Chaves M, Cope F, et al. Mannose receptor high, M2 dermal macrophages mediate nonhealing Leishmania major infection in a Th1 immune environment. J Exp Med. 2018;215:357-375 pubmed publisher
	Giampazolias E, Zunino B, Dhayade S, Bock F, Cloix C, Cao K, et al. Mitochondrial permeabilization engages NF-κB-dependent anti-tumour activity under caspase deficiency. Nat Cell Biol. 2017;19:1116-1129 pubmed publisher
	Jin W, Shi S, Li Z, Li M, Wood K, Gonzales R, et al. Depletion of microglia exacerbates postischemic inflammation and brain injury. J Cereb Blood Flow Metab. 2017;37:2224-2236 pubmed publisher
	Jin R, Blair S, Warunek J, Heffner R, Blader I, Wohlfert E. Regulatory T Cells Promote Myositis and Muscle Damage in Toxoplasma gondii Infection. J Immunol. 2017;198:352-362 pubmed
	Yuan M, Li D, An M, Li Q, Zhang L, Wang G. Rediscovering peritoneal macrophages in a murine endometriosis model. Hum Reprod. 2017;32:94-102 pubmed
	Nalbandian A, Khan A, Srivastava R, Llewellyn K, Tan B, Shukr N, et al. Activation of the NLRP3 Inflammasome Is Associated with Valosin-Containing Protein Myopathy. Inflammation. 2017;40:21-41 pubmed publisher
	Ren K, Jin C, Ma P, Ren Q, Jia Z, Zhu D. Ginsenoside Rd alleviates mouse acute renal ischemia/reperfusion injury by modulating macrophage phenotype. J Ginseng Res. 2016;40:196-202 pubmed publisher
	Schleicher U, Paduch K, Debus A, Obermeyer S, König T, Kling J, et al. TNF-Mediated Restriction of Arginase 1 Expression in Myeloid Cells Triggers Type 2 NO Synthase Activity at the Site of Infection. Cell Rep. 2016;15:1062-1075 pubmed publisher
	Smolders S, Smolders S, Swinnen N, GÃ¤rtner A, Rigo J, Legendre P, et al. Maternal immune activation evoked by polyinosinic:polycytidylic acid does not evoke microglial cell activation in the embryo. Front Cell Neurosci. 2015;9:301 pubmed publisher
	Jin X, Yao T, Zhou Z, Zhu J, Zhang S, Hu W, et al. Advanced Glycation End Products Enhance Macrophages Polarization into M1 Phenotype through Activating RAGE/NF-ÎºB Pathway. Biomed Res Int. 2015;2015:732450 pubmed publisher
	Shen L, Sundstedt A, Ciesielski M, Miles K, Celander M, Adelaiye R, et al. Tasquinimod modulates suppressive myeloid cells and enhances cancer immunotherapies in murine models. Cancer Immunol Res. 2015;3:136-48 pubmed publisher
	Nakanishi Y, Sato T, Ohteki T. Commensal Gram-positive bacteria initiates colitis by inducing monocyte/macrophage mobilization. Mucosal Immunol. 2015;8:152-60 pubmed publisher

product information

Product Type :

Antibody

Product Name :

iNOS Monoclonal Antibody (CXNFT), Alexa Fluor™ 488, eBioscience

Catalog # :

53-5920-82

Quantity :

100 µg

Price :

US 383.00

Clonality :

Monoclonal

Purity :

Affinity chromatography

Host :

Rat

Reactivity :

Mouse

Applications :

Flow Cytometry: 0.06 µg/test, Immunocytochemistry: 1:100, Western Blot: 1:1,000

Species :

Mouse

Clone :

CXNFT

Isotype :

IgG2a, kappa

Storage :

4° C, store in dark, DO NOT FREEZE!

Description :

iNOS (Inducible Nitric oxide, NO, NOS) is an inorganic, gaseous free radical that carries a variety of messages between cells. Vasorelaxation, neurotransmission and cytotoxicity can all be potentiated through cellular response to NO. NO production is mediated by members of the nitric oxide synthase (NOS) family. iNOS is expressed in liver and inducible by a combination of lipopolysaccharide and certain cytokines. NOS catalyzes the oxidization of L-arginine to produce L-citrulline and NO. Two constitutive isoforms, brain or neuronal NOS (b or nNOS, type I) and endothelial cell NOS (eNOS, type III), and one inducible isoform (iNOS, type II), have been cloned. All NOS isoforms contain calmodulin, nicotinamide adenine dinucleotide phosphate (NADPH), flavin adenine dinucleotide (FAD), and flavin mononucleotide (FMN) binding domains. iNOS is found in a variety of cell types including macrophages, hepatocytes, synoviocytes, and smooth muscle cells. Cytokines such as interferon-gamma (IFN), tumor necrosis factor (TNF), interleukin-1 and -2, and lipopolysaccarides (LPS) cause an increase in iNOS mRNA, protein, and activity levels. Protein kinase C-stimulating agents exhibit the same effect on iNOS activity. After cytokine induction, iNOS exhibits a delayed activity response which is then followed by a significant increase in NO production over a long period of time. Three related iNOS pseudogenes are located within the Smith-Magenis syndrome region on chromosome 17. Diseases associated with iNOS dysfunction include achalasia and impotence.

Format :

Liquid

Applications w/Dilutions :

Flow Cytometry: 0.06 µg/test, Immunocytochemistry: 1:100, Western Blot: 1:1,000

Aliases :

Hepatocyte NOS; hepatocytes; HEP-NOS; inducible nitric oxide synthase; inducible NO synthase; Inducible NOS; iNos; i-NOS; Inosl; MAC-NOS; macrophage NOS; nitric oxide synthase 2; nitric oxide synthase 2, inducible; nitric oxide synthase 2, inducible, macrophage; nitric oxide synthase 2A (inducible, hepatocytes); nitric oxide synthase, inducible; nitric oxide synthase, macrophage; nitric oxide synthase-inducible; NOS; NOS type II; NOS, type II; Nos2; Nos-2; Nos2a; NOS-II; OTTMUSP00000000202; peptidyl-cysteine S-nitrosylase NOS2

more info or order :

Invitrogen product webpage