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company name :
Invitrogen
other brands :
NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience
product type :
antibody
product name :
Nanog Monoclonal Antibody (eBioMLC-51), Alexa Fluor™ 488, eBioscience
catalog :
53-5761-80
quantity :
25 µg
price :
US 327.00
clonality :
monoclonal
host :
rat
conjugate :
AF488
clone name :
eBioMLC-51
reactivity :
mouse
application :
immunocytochemistry, flow cytometry
more info or order :
citations: 47
Published Application/Species/Sample/DilutionReference
  • immunocytochemistry; mouse; 1:50; loading ...; tbl 2
Li Y, Adomat H, Guns E, Hojabrpour P, Duronio V, Curran T, et al. Identification of a Hematopoietic Cell Dedifferentiation-Inducing Factor. J Cell Physiol. 2016;231:1350-63 pubmed publisher
  • immunocytochemistry; mouse
Galvagni F, Lentucci C, Neri F, Dettori D, De Clemente C, Orlandini M, et al. Snai1 promotes ESC exit from the pluripotency by direct repression of self-renewal genes. Stem Cells. 2015;33:742-50 pubmed publisher
Ohishi H, Shimada S, Uchino S, Li J, Sato Y, Shintani M, et al. STREAMING-tag system reveals spatiotemporal relationships between transcriptional regulatory factors and transcriptional activity. Nat Commun. 2022;13:7672 pubmed publisher
Westerling Bui A, Fast E, Soare T, Venkatachalan S, DeRan M, Fanelli A, et al. Transplanted organoids empower human preclinical assessment of drug candidate for the clinic. Sci Adv. 2022;8:eabj5633 pubmed publisher
Haraguchi D, Nakamura T. Pramef12 enhances reprogramming into naïve iPS cells. Biochem Biophys Rep. 2022;30:101267 pubmed publisher
Han D, Wu G, Chen R, Drexler H, MacCarthy C, Kim K, et al. A balanced Oct4 interactome is crucial for maintaining pluripotency. Sci Adv. 2022;8:eabe4375 pubmed publisher
Zuo Z, Yang G, Wang H, Liu S, Zhang Y, Cai Y, et al. Klf4 methylated by Prmt1 restrains the commitment of primitive endoderm. Nucleic Acids Res. 2022;50:2005-2018 pubmed publisher
Raina D, Bahadori A, Stanoev A, Protzek M, Koseska A, Schr xf6 ter C. Cell-cell communication through FGF4 generates and maintains robust proportions of differentiated cell types in embryonic stem cells. Development. 2021;148: pubmed publisher
Xu P, Borges R, Fillatre J, de Oliveira Melo M, Cheng T, Thisse B, et al. Construction of a mammalian embryo model from stem cells organized by a morphogen signalling centre. Nat Commun. 2021;12:3277 pubmed publisher
Kinoshita M, Barber M, Mansfield W, Cui Y, Spindlow D, Stirparo G, et al. Capture of Mouse and Human Stem Cells with Features of Formative Pluripotency. Cell Stem Cell. 2021;28:453-471.e8 pubmed publisher
Bergert M, Lembo S, Sharma S, Russo L, Milovanović D, Gretarsson K, et al. Cell Surface Mechanics Gate Embryonic Stem Cell Differentiation. Cell Stem Cell. 2021;28:209-216.e4 pubmed publisher
Ochiai H, Hayashi T, Umeda M, Yoshimura M, Harada A, Shimizu Y, et al. Genome-wide kinetic properties of transcriptional bursting in mouse embryonic stem cells. Sci Adv. 2020;6:eaaz6699 pubmed publisher
Rao C, Malaguti M, Mason J, Lowell S. The transcription factor E2A drives neural differentiation in pluripotent cells. Development. 2020;147: pubmed publisher
Fischer S, Corujo Simon E, Lilao Garzon J, Stelzer E, Muñoz Descalzo S. The transition from local to global patterns governs the differentiation of mouse blastocysts. PLoS ONE. 2020;15:e0233030 pubmed publisher
Pantier R, Tatar T, Colby D, Chambers I. Endogenous epitope-tagging of Tet1, Tet2 and Tet3 identifies TET2 as a naïve pluripotency marker. Life Sci Alliance. 2019;2: pubmed publisher
Vanheer L, Song J, De Geest N, Janiszewski A, Talon I, Provenzano C, et al. Tox4 modulates cell fate reprogramming. J Cell Sci. 2019;132: pubmed publisher
Vardhana S, Arnold P, Rosen B, Chen Y, Carey B, Huangfu D, et al. Glutamine independence is a selectable feature of pluripotent stem cells. Nat Metab. 2019;1:676-687 pubmed publisher
Stuart H, Stirparo G, Lohoff T, Bates L, Kinoshita M, Lim C, et al. Distinct Molecular Trajectories Converge to Induce Naive Pluripotency. Cell Stem Cell. 2019;25:388-406.e8 pubmed publisher
Yachie Kinoshita A, Onishi K, Ostblom J, Langley M, Pósfai E, Rossant J, et al. Modeling signaling-dependent pluripotency with Boolean logic to predict cell fate transitions. Mol Syst Biol. 2018;14:e7952 pubmed publisher
Li M, Amaral P, Cheung P, Bergmann J, Kinoshita M, Kalkan T, et al. A lncRNA fine tunes the dynamics of a cell state transition involving Lin28, let-7 and de novo DNA methylation. elife. 2017;6: pubmed publisher
Nissen S, Perera M, Gonzalez J, Morgani S, Jensen M, Sneppen K, et al. Four simple rules that are sufficient to generate the mammalian blastocyst. PLoS Biol. 2017;15:e2000737 pubmed publisher
Jang S, Choubey S, Furchtgott L, Zou L, Doyle A, Menon V, et al. Dynamics of embryonic stem cell differentiation inferred from single-cell transcriptomics show a series of transitions through discrete cell states. elife. 2017;6: pubmed publisher
Thamodaran V, Bruce A. p38 (Mapk14/11) occupies a regulatory node governing entry into primitive endoderm differentiation during preimplantation mouse embryo development. Open Biol. 2016;6: pubmed publisher
Martin Gonzalez J, Morgani S, Bone R, Bonderup K, Abelchian S, Brakebusch C, et al. Embryonic Stem Cell Culture Conditions Support Distinct States Associated with Different Developmental Stages and Potency. Stem Cell Reports. 2016;7:177-91 pubmed publisher
Murakami K, Günesdogan U, Zylicz J, Tang W, Sengupta R, Kobayashi T, et al. NANOG alone induces germ cells in primed epiblast in vitro by activation of enhancers. Nature. 2016;529:403-407 pubmed publisher
Alexandrova S, Kalkan T, Humphreys P, Riddell A, Scognamiglio R, Trumpp A, et al. Selection and dynamics of embryonic stem cell integration into early mouse embryos. Development. 2016;143:24-34 pubmed publisher
Schroter C, Rué P, Mackenzie J, Martinez Arias A. FGF/MAPK signaling sets the switching threshold of a bistable circuit controlling cell fate decisions in embryonic stem cells. Development. 2015;142:4205-16 pubmed publisher
Economou C, Tsakiridis A, Wymeersch F, Gordon Keylock S, Dewhurst R, Fisher D, et al. Intrinsic factors and the embryonic environment influence the formation of extragonadal teratomas during gestation. BMC Dev Biol. 2015;15:35 pubmed publisher
Chantzoura E, Skylaki S, Menendez S, Kim S, Johnsson A, Linnarsson S, et al. Reprogramming Roadblocks Are System Dependent. Stem Cell Reports. 2015;5:350-64 pubmed publisher
Pasque V, Tchieu J, Karnik R, Uyeda M, Sadhu Dimashkie A, Case D, et al. X chromosome reactivation dynamics reveal stages of reprogramming to pluripotency. Cell. 2014;159:1681-97 pubmed publisher
Gouti M, Tsakiridis A, Wymeersch F, Huang Y, Kleinjung J, Wilson V, et al. In vitro generation of neuromesodermal progenitors reveals distinct roles for wnt signalling in the specification of spinal cord and paraxial mesoderm identity. PLoS Biol. 2014;12:e1001937 pubmed publisher
Doughton G, Wei J, Tapon N, Welham M, Chalmers A. Formation of a polarised primitive endoderm layer in embryoid bodies requires fgfr/erk signalling. PLoS ONE. 2014;9:e95434 pubmed publisher
Tsakiridis A, Huang Y, Blin G, Skylaki S, Wymeersch F, Osorno R, et al. Distinct Wnt-driven primitive streak-like populations reflect in vivo lineage precursors. Development. 2014;141:1209-21 pubmed publisher
Le Bin G, Muñoz Descalzo S, Kurowski A, Leitch H, Lou X, Mansfield W, et al. Oct4 is required for lineage priming in the developing inner cell mass of the mouse blastocyst. Development. 2014;141:1001-10 pubmed publisher
Huijbers I, Bin Ali R, Pritchard C, Cozijnsen M, Kwon M, Proost N, et al. Rapid target gene validation in complex cancer mouse models using re-derived embryonic stem cells. EMBO Mol Med. 2014;6:212-25 pubmed publisher
Payer B, Rosenberg M, Yamaji M, Yabuta Y, Koyanagi Aoi M, Hayashi K, et al. Tsix RNA and the germline factor, PRDM14, link X reactivation and stem cell reprogramming. Mol Cell. 2013;52:805-18 pubmed publisher
Denholtz M, Bonora G, Chronis C, Splinter E, de Laat W, Ernst J, et al. Long-range chromatin contacts in embryonic stem cells reveal a role for pluripotency factors and polycomb proteins in genome organization. Cell Stem Cell. 2013;13:602-16 pubmed publisher
Kumar R, Dimenna L, Schrode N, Liu T, Franck P, Muñoz Descalzo S, et al. AID stabilizes stem-cell phenotype by removing epigenetic memory of pluripotency genes. Nature. 2013;500:89-92 pubmed publisher
Morgani S, Canham M, Nichols J, Sharov A, Migueles R, Ko M, et al. Totipotent embryonic stem cells arise in ground-state culture conditions. Cell Rep. 2013;3:1945-57 pubmed publisher
Radzisheuskaya A, Chia G, Dos Santos R, Theunissen T, Castro L, Nichols J, et al. A defined Oct4 level governs cell state transitions of pluripotency entry and differentiation into all embryonic lineages. Nat Cell Biol. 2013;15:579-90 pubmed publisher
Faunes F, Hayward P, Descalzo S, Chatterjee S, Balayo T, Trott J, et al. A membrane-associated β-catenin/Oct4 complex correlates with ground-state pluripotency in mouse embryonic stem cells. Development. 2013;140:1171-83 pubmed publisher
Pasque V, Radzisheuskaya A, Gillich A, Halley Stott R, Panamarova M, Zernicka Goetz M, et al. Histone variant macroH2A marks embryonic differentiation in vivo and acts as an epigenetic barrier to induced pluripotency. J Cell Sci. 2012;125:6094-104 pubmed publisher
Muñoz Descalzo S, Rué P, Garcia Ojalvo J, Martinez Arias A. Correlations between the levels of Oct4 and Nanog as a signature for naïve pluripotency in mouse embryonic stem cells. Stem Cells. 2012;30:2683-91 pubmed publisher
Marks H, Kalkan T, Menafra R, Denissov S, Jones K, Hofemeister H, et al. The transcriptional and epigenomic foundations of ground state pluripotency. Cell. 2012;149:590-604 pubmed publisher
Wray J, Kalkan T, Gómez López S, Eckardt D, Cook A, Kemler R, et al. Inhibition of glycogen synthase kinase-3 alleviates Tcf3 repression of the pluripotency network and increases embryonic stem cell resistance to differentiation. Nat Cell Biol. 2011;13:838-45 pubmed publisher
Guo G, Huang Y, Humphreys P, Wang X, Smith A. A PiggyBac-based recessive screening method to identify pluripotency regulators. PLoS ONE. 2011;6:e18189 pubmed publisher
Wang Y, Fan Y, Puga A. Dioxin exposure disrupts the differentiation of mouse embryonic stem cells into cardiomyocytes. Toxicol Sci. 2010;115:225-37 pubmed publisher
product information
Product Type :
Antibody
Product Name :
Nanog Monoclonal Antibody (eBioMLC-51), Alexa Fluor™ 488, eBioscience
Catalog # :
53-5761-80
Quantity :
25 µg
Price :
US 327.00
Clonality :
Monoclonal
Purity :
Affinity chromatography
Host :
Rat
Reactivity :
Mouse
Applications :
Flow Cytometry: 0.06 µg/test, Immunocytochemistry: 10 µg/mL
Species :
Mouse
Clone :
eBioMLC-51
Isotype :
IgG2a, kappa
Storage :
4° C, store in dark, DO NOT FREEZE!
Description :
NANOG (Nanog homeobox) is a divergent homeodomain protein that directs pluripotency and differentiation of undifferentiated embryonic stem cells. NANOG mRNA is present in pluripotent mouse and human cell lines, and absent from differentiated cells. Human NANOG protein shares 52% overall amino acid identity with the mouse protein, and 85% identity in the homeodomain. Human NANOG maps to gene locus 12p13.31, while the mouse NANOG maps to gene loci 6 F2. Murine embryonic NANOG expression is detected in the inner cell mass of the blastocyst. Research studies have shown that high levels of human NANOG expression in the undifferentiated N-Tera embryonal carcinoma cell line. Further, NANOG is a transcription regulator involved in inner cell mass and embryonic stem (ES) cells proliferation and self-renewal. The role of NANOG in embryonic development suggested that it might be useful in the creation of stem cells that might be useful in cell replacement therapies in the treatment of several degenerative diseases. Artificial stem cells, termed induced pluripotent stem (iPS) cells, can be created by expressing POU5F1 (also known as Oct-4), another germline-specific transcription factor, and the transcription factors Sox2, Klf4 and Lin28 along with c-Myc in mouse fibroblasts. Experiments have demonstrated that iPS cells could be generated using expression plasmids expressing NANOG, Sox2, KlfF4 and c-Myc, eliminating the need for virus introduction, thereby addressing a safety concern for potential use of iPS cells in regenerative medicine. When overexpressed, NANOG promotes cells to enter into S phase and proliferation. Diseases associated with dysfunction in the NANOG protein include tetracarcinoma and germ cell/embryonal cancer.
Format :
Liquid
Applications w/Dilutions :
Flow Cytometry: 0.06 µg/test, Immunocytochemistry: 10 µg/mL
Aliases :
2410002E02Rik; early embryo specific expression NK family; early embryo specific expression NK-type homeobox protein; Ecat4; ENK; ES cell-associated protein 4; FLJ12581; HGNC:20857; hNanog; Homeobox protein NANOG; homeobox transcription factor Nanog; homeobox transcription factor Nanog-delta 48; Nanog; Nanog homeobox
more info or order :
company information
Invitrogen
Thermo Fisher Scientific
81 Wyman Street
Waltham, MA USA 02451
https://www.thermofisher.com
800-678-5599
headquarters: USA