product summary
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company name :
Invitrogen
other brands :
NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience
product type :
antibody
product name :
F4/80 Monoclonal Antibody (BM8), Alexa Fluor™ 488, eBioscience
catalog :
53-4801-82
quantity :
100 µg
price :
US 356
clonality :
monoclonal
host :
rat
conjugate :
AF488
clone name :
BM8
reactivity :
mouse
application :
flow cytometry
more info or order :
citations: 365
Published Application/Species/Sample/DilutionReference
  • flow cytometry; mouse; loading ...; fig s6a
Félix I, Jokela H, Karhula J, Kotaja N, Savontaus E, Salmi M, et al. Single-Cell Proteomics Reveals the Defined Heterogeneity of Resident Macrophages in White Adipose Tissue. Front Immunol. 2021;12:719979 pubmed publisher
  • flow cytometry; mouse; 1:20; fig 1
Shiraishi M, Shintani Y, Shintani Y, Ishida H, Saba R, Yamaguchi A, et al. Alternatively activated macrophages determine repair of the infarcted adult murine heart. J Clin Invest. 2016;126:2151-66 pubmed publisher
  • flow cytometry; mouse; fig s21
Ying W, Tseng A, Chang R, Wang H, Lin Y, Kanameni S, et al. miR-150 regulates obesity-associated insulin resistance by controlling B cell functions. Sci Rep. 2016;6:20176 pubmed publisher
  • flow cytometry; mouse; fig 5
Liu J, Zhang N, Li Q, Zhang W, Ke F, Leng Q, et al. Tumor-associated macrophages recruit CCR6+ regulatory T cells and promote the development of colorectal cancer via enhancing CCL20 production in mice. PLoS ONE. 2011;6:e19495 pubmed publisher
Duan H, Jing L, Xiang J, Ju C, Wu Z, Liu J, et al. CD146 Associates with Gp130 to Control a Macrophage Pro-inflammatory Program That Regulates the Metabolic Response to Obesity. Adv Sci (Weinh). 2022;9:e2103719 pubmed publisher
Azuma Y, Fujita T, Izawa T, Hirota K, Nishiyama K, Ikegami A, et al. IL-19 Contributes to the Development of Nonalcoholic Steatohepatitis by Altering Lipid Metabolism. Cells. 2021;10: pubmed publisher
Medina Buelvas D, Rodr xed guez Sosa M, Vega L. Characterisation of Macrophage Polarisation in Mice Infected with Ninoa Strain of Trypanosoma cruzi. Pathogens. 2021;10: pubmed publisher
Ji W, Li D, Wei D, Gu A, Yang Y, Peng J. Cytochrome P450 26A1 Modulates the Polarization of Uterine Macrophages During the Peri-Implantation Period. Front Immunol. 2021;12:763067 pubmed publisher
Zhu X, Liu J, Pan H, Geng Z, Huang W, Liu T, et al. Thymopentin treatment of murine premature ovarian failure via attenuation of immune cell activity and promotion of the BMP4/Smad9 signalling pathway. Int J Med Sci. 2021;18:3544-3555 pubmed publisher
Oladiran O, Shi X, Fournier S, Zhang J. CX3CR1 But Not CCR2 Expression Is Required for the Development of Autoimmune Peripheral Neuropathy in Mice. Front Immunol. 2021;12:720733 pubmed publisher
Chow S, Cui C, Cheng K, Chim Y, Wang J, Wong C, et al. Acute Inflammatory Response in Osteoporotic Fracture Healing Augmented with Mechanical Stimulation is Regulated In Vivo through the p38-MAPK Pathway. Int J Mol Sci. 2021;22: pubmed publisher
DORA D, Ferenczi S, Stavely R, Tóth V, Varga Z, Kovacs T, et al. Evidence of a Myenteric Plexus Barrier and Its Macrophage-Dependent Degradation During Murine Colitis: Implications in Enteric Neuroinflammation. Cell Mol Gastroenterol Hepatol. 2021;12:1617-1641 pubmed publisher
Cheng Y, Zhang Z, Lan B, Lin J, Chen X, Kong L, et al. PDGF-D activation by macrophage-derived uPA promotes AngII-induced cardiac remodeling in obese mice. J Exp Med. 2021;218: pubmed publisher
Bereswill S, Mousavi S, Weschka D, Heimesaat M. Disease-Alleviating Effects of Peroral Activated Charcoal Treatment in Acute Murine Campylobacteriosis. Microorganisms. 2021;9: pubmed publisher
Heimesaat M, Weschka D, Mousavi S, Bereswill S. Treatment with the Probiotic Product Aviguard® Alleviates Inflammatory Responses during Campylobacter jejuni-Induced Acute Enterocolitis in Mice. Int J Mol Sci. 2021;22: pubmed publisher
Cai C, Zeng D, Gao Q, Ma L, Zeng B, Zhou Y, et al. Decreased ferroportin in hepatocytes promotes macrophages polarize towards an M2-like phenotype and liver fibrosis. Sci Rep. 2021;11:13386 pubmed publisher
Shan Z, Li L, Atkins C, Wang M, Wen Y, Jeong J, et al. Chitinase 3-like-1 contributes to acetaminophen-induced liver injury by promoting hepatic platelet recruitment. elife. 2021;10: pubmed publisher
Hridi S, Barbour M, Wilson C, Franssen A, Harte T, Bushell T, et al. Increased Levels of IL-16 in the Central Nervous System during Neuroinflammation Are Associated with Infiltrating Immune Cells and Resident Glial Cells. Biology (Basel). 2021;10: pubmed publisher
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Bieniasz Krzywiec P, Martín Pérez R, Riera Domingo C, Mazzone M. Isolation and separation of murine tumor-associated macrophages (TAMs) subpopulations from orthotopic 4T1 breast tumors. STAR Protoc. 2021;2:100481 pubmed publisher
Frenis K, Helmstädter J, Ruan Y, Schramm E, Kalinovic S, Kröller Schön S, et al. Ablation of lysozyme M-positive cells prevents aircraft noise-induced vascular damage without improving cerebral side effects. Basic Res Cardiol. 2021;116:31 pubmed publisher
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Bereswill S, Mousavi S, Weschka D, Buczkowski A, Schmidt S, Heimesaat M. Peroral Clove Essential Oil Treatment Ameliorates Acute Campylobacteriosis-Results from a Preclinical Murine Intervention Study. Microorganisms. 2021;9: pubmed publisher
Oladiran O, Shi X, Yang M, Fournier S, Zhang J. Inhibition of TLR4 signaling protects mice from sensory and motor dysfunction in an animal model of autoimmune peripheral neuropathy. J Neuroinflammation. 2021;18:77 pubmed publisher
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Wieghofer P, Hagemeyer N, Sankowski R, Schlecht A, Staszewski O, Amann L, et al. Mapping the origin and fate of myeloid cells in distinct compartments of the eye by single-cell profiling. EMBO J. 2021;40:e105123 pubmed publisher
Vonderhaar E, Barnekow N, McAllister D, McOlash L, Eid M, Riese M, et al. STING Activated Tumor-Intrinsic Type I Interferon Signaling Promotes CXCR3 Dependent Antitumor Immunity in Pancreatic Cancer. Cell Mol Gastroenterol Hepatol. 2021;12:41-58 pubmed publisher
Kusakabe J, Hata K, Miyauchi H, Tajima T, Wang Y, Tamaki I, et al. Complement-5 Inhibition Deters Progression of Fulminant Hepatitis to Acute Liver Failure in Murine Models. Cell Mol Gastroenterol Hepatol. 2021;11:1351-1367 pubmed publisher
Yousif A, Ronsard L, Shah P, Omatsu T, Sangesland M, Bracamonte Moreno T, et al. The persistence of interleukin-6 is regulated by a blood buffer system derived from dendritic cells. Immunity. 2021;54:235-246.e5 pubmed publisher
Zhang G, Jiao Q, Shen C, Song H, Zhang H, Qiu Z, et al. Interleukin 6 regulates the expression of programmed cell death ligand 1 in thyroid cancer. Cancer Sci. 2021;112:997-1010 pubmed publisher
Gula G, Ruminski S, Niderla Bielinska J, Jasińska A, Kiernozek E, Jankowska Steifer E, et al. Potential functions of embryonic cardiac macrophages in angiogenesis, lymphangiogenesis and extracellular matrix remodeling. Histochem Cell Biol. 2021;155:117-132 pubmed publisher
Jin Y, Ji Y, Song Y, Choe S, Jeon Y, Na H, et al. Depletion of Adipocyte Becn1 Leads to Lipodystrophy and Metabolic Dysregulation. Diabetes. 2021;70:182-195 pubmed publisher
Zhao Q, Shi M, Yin C, Zhao Z, Zhang J, Wang J, et al. Dual-Wavelength Photosensitive Nano-in-Micro Scaffold Regulates Innate and Adaptive Immune Responses for Osteogenesis. Nanomicro Lett. 2020;13:28 pubmed publisher
Mousavi S, Weschka D, Bereswill S, Heimesaat M. Preclinical Evaluation of Oral Urolithin-A for the Treatment of Acute Campylobacteriosis in Campylobacter jejuni Infected Microbiota-Depleted IL-10-/- Mice. Pathogens. 2020;10: pubmed publisher
Liu Q, Xie Z, Qiu M, Shim I, Yang Y, Xie S, et al. Prodrug-Loaded Zirconium Carbide Nanosheets as a Novel Biophotonic Nanoplatform for Effective Treatment of Cancer. Adv Sci (Weinh). 2020;7:2001191 pubmed publisher
Ding X, Wang S, Wang Y, Yang J, Bao N, Liu J, et al. Neonatal Heart Responds to Pressure Overload With Differential Alterations in Various Cardiomyocyte Maturation Programs That Accommodate Simultaneous Hypertrophy and Hyperplasia. Front Cell Dev Biol. 2020;8:596960 pubmed publisher
Hadas R, Gershon E, Cohen A, Atrakchi O, Lazar S, Golani O, et al. Hyaluronan control of the primary vascular barrier during early mouse pregnancy is mediated by uterine NK cells. JCI Insight. 2020;5: pubmed publisher
Jin J, Wang T, Park W, Li W, Kim W, Park S, et al. Inhibition of Yes-Associated Protein by Verteporfin Ameliorates Unilateral Ureteral Obstruction-Induced Renal Tubulointerstitial Inflammation and Fibrosis. Int J Mol Sci. 2020;21: pubmed publisher
Pavlovic N, Kopsida M, Gerwins P, Heindryckx F. Inhibiting P2Y12 in Macrophages Induces Endoplasmic Reticulum Stress and Promotes an Anti-Tumoral Phenotype. Int J Mol Sci. 2020;21: pubmed publisher
Feng Y, Guo F, Xia Z, Liu J, Mai H, Liang Y, et al. Inhibition of Fatty Acid-Binding Protein 4 Attenuated Kidney Fibrosis by Mediating Macrophage-to-Myofibroblast Transition. Front Immunol. 2020;11:566535 pubmed publisher
Schilke R, Blackburn C, Rao S, Krzywanski D, Finck B, Woolard M. Macrophage-Associated Lipin-1 Promotes β-Oxidation in Response to Proresolving Stimuli. Immunohorizons. 2020;4:659-669 pubmed publisher
Wang Y, Han B, Wang Y, Wang C, Zhang H, Xue J, et al. Mesenchymal stem cell-secreted extracellular vesicles carrying TGF-β1 up-regulate miR-132 and promote mouse M2 macrophage polarization. J Cell Mol Med. 2020;24:12750-12764 pubmed publisher
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Krenacs T, Meggyeshazi N, Forika G, Kiss E, Hamar P, Székely T, et al. Modulated Electro-Hyperthermia-Induced Tumor Damage Mechanisms Revealed in Cancer Models. Int J Mol Sci. 2020;21: pubmed publisher
Tang P, Zhang Y, Xiao J, Tang P, Chung J, Li J, et al. Neural transcription factor Pou4f1 promotes renal fibrosis via macrophage-myofibroblast transition. Proc Natl Acad Sci U S A. 2020;117:20741-20752 pubmed publisher
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Lu C, Shiau A, Su B, Hsu T, Wang C, Su Y, et al. Oct4 promotes M2 macrophage polarization through upregulation of macrophage colony-stimulating factor in lung cancer. J Hematol Oncol. 2020;13:62 pubmed publisher
Jiang M, Ye J, Wang X, Li N, Wang Y, Shi Y. Phosphatase SHP1 impedes mesenchymal stromal cell immunosuppressive capacity modulated by JAK1/STAT3 and P38 signals. Cell Biosci. 2020;10:65 pubmed publisher
Heimesaat M, Mousavi S, Kløve S, Genger C, Weschka D, Giladi E, et al. Immune-modulatory Properties of the Octapeptide NAP in Campylobacter jejuni Infected Mice Suffering from Acute Enterocolitis. Microorganisms. 2020;8: pubmed publisher
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Chandran S, Schilke R, Blackburn C, Yurochko A, Mirza R, Scott R, et al. Lipin-1 Contributes to IL-4 Mediated Macrophage Polarization. Front Immunol. 2020;11:787 pubmed publisher
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Wise L, Stuart G, Jones N, Fleming S, Mercer A. Orf Virus IL-10 and VEGF-E Act Synergistically to Enhance Healing of Cutaneous Wounds in Mice. J Clin Med. 2020;9: pubmed publisher
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Cheng R, Billet S, Liu C, Haldar S, Choudhury D, Tripathi M, et al. Periodontal inflammation recruits distant metastatic breast cancer cells by increasing myeloid-derived suppressor cells. Oncogene. 2020;39:1543-1556 pubmed publisher
Zhao X, Chen G, Liu Y, Chen Y, Wu H, Chen J, et al. Inhibitory effect of silymarin on CCl4-induced liver fibrosis by reducing Ly6Chi monocytes infiltration. Int J Clin Exp Pathol. 2017;10:11941-11951 pubmed
Hasan S, Johnson M, Kini A, Baldea A, MUTHUMALAIAPPAN K. A Shift in Myeloid Cell Phenotype via Down Regulation of Siglec-1 in Island Macrophages of Bone Marrow Is Associated With Decreased Late Erythroblasts Seen in Anemia of Critical Illness. Front Med (Lausanne). 2019;6:260 pubmed publisher
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Caution K, Young N, Robledo Avila F, Krause K, Abu Khweek A, Hamilton K, et al. Caspase-11 Mediates Neutrophil Chemotaxis and Extracellular Trap Formation During Acute Gouty Arthritis Through Alteration of Cofilin Phosphorylation. Front Immunol. 2019;10:2519 pubmed publisher
Wang S, Liu X, Huang R, Zheng Y, Wang N, Yang B, et al. XIAOPI Formula Inhibits Breast Cancer Stem Cells via Suppressing Tumor-Associated Macrophages/C-X-C Motif Chemokine Ligand 1 Pathway. Front Pharmacol. 2019;10:1371 pubmed publisher
Fang D, Tang Q, Kong Y, Wang Q, Gu J, Fang X, et al. MDM2 inhibitor APG-115 synergizes with PD-1 blockade through enhancing antitumor immunity in the tumor microenvironment. J Immunother Cancer. 2019;7:327 pubmed publisher
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Heimesaat M, Mrazek K, Bereswill S. Murine Fecal Microbiota Transplantation Alleviates Intestinal and Systemic Immune Responses in Campylobacter jejuni Infected Mice Harboring a Human Gut Microbiota. Front Immunol. 2019;10:2272 pubmed publisher
Mills B, Connolly K, Ye J, Murphy J, Uccello T, Han B, et al. Stereotactic Body Radiation and Interleukin-12 Combination Therapy Eradicates Pancreatic Tumors by Repolarizing the Immune Microenvironment. Cell Rep. 2019;29:406-421.e5 pubmed publisher
Ershaid N, Sharon Y, Doron H, Raz Y, Shani O, Cohen N, et al. NLRP3 inflammasome in fibroblasts links tissue damage with inflammation in breast cancer progression and metastasis. Nat Commun. 2019;10:4375 pubmed publisher
Mousavi S, Lobo de Sá F, Schulzke J, Bücker R, Bereswill S, Heimesaat M. Vitamin D in Acute Campylobacteriosis-Results From an Intervention Study Applying a Clinical Campylobacter jejuni Induced Enterocolitis Model. Front Immunol. 2019;10:2094 pubmed publisher
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Gautam A, Park B, Kim T, Akauliya M, Kim D, Maharjan S, et al. Peritoneal Cells Mediate Immune Responses and Cross-Protection Against Influenza A Virus. Front Immunol. 2019;10:1160 pubmed publisher
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Ohashi K, Wang Z, Yang Y, Billet S, Tu W, Pimienta M, et al. NOD-like receptor C4 Inflammasome Regulates the Growth of Colon Cancer Liver Metastasis in NAFLD. Hepatology. 2019;70:1582-1599 pubmed publisher
Zhou Y, Fu C, Kong Y, Pan D, Wang Y, Huang S, et al. Antitumor and immunomodulatory effects of a novel multitarget inhibitor, CS2164, in mouse hepatocellular carcinoma models. Anticancer Drugs. 2019;30:909-916 pubmed publisher
Kurelac I, Iommarini L, Vatrinet R, Amato L, De Luise M, Leone G, et al. Inducing cancer indolence by targeting mitochondrial Complex I is potentiated by blocking macrophage-mediated adaptive responses. Nat Commun. 2019;10:903 pubmed publisher
Zheng X, Zhang A, Binnie M, McGuire K, Webster S, Hughes J, et al. Kynurenine 3-monooxygenase is a critical regulator of renal ischemia-reperfusion injury. Exp Mol Med. 2019;51:15 pubmed publisher
Viitala M, Virtakoivu R, Tadayon S, Rannikko J, Jalkanen S, Hollmen M. Immunotherapeutic Blockade of Macrophage Clever-1 Reactivates the CD8+ T-cell Response against Immunosuppressive Tumors. Clin Cancer Res. 2019;25:3289-3303 pubmed publisher
Tellez Freitas C, Burrell H, Valdoz J, Hamblin G, Raymond C, Cox T, et al. The nuclear variant of bone morphogenetic protein 2 (nBMP2) is expressed in macrophages and alters calcium response. Sci Rep. 2019;9:934 pubmed publisher
Zha J, Li H, Lin Q, Kuo W, Jiang Z, Tsai P, et al. Interleukin 22 Expands Transit-Amplifying Cells While Depleting Lgr5+ Stem Cells via Inhibition of Wnt and Notch Signaling. Cell Mol Gastroenterol Hepatol. 2019;7:255-274 pubmed publisher
Jin H, Zhang Y, Ding Q, Wang S, Rastogi P, Dai D, et al. Epithelial innate immunity mediates tubular cell senescence after kidney injury. JCI Insight. 2019;4: pubmed publisher
Farooq M, Filliol A, Simoes Eugénio M, Piquet Pellorce C, Dion S, Raguénès Nicol C, et al. Depletion of RIPK1 in hepatocytes exacerbates liver damage in fulminant viral hepatitis. Cell Death Dis. 2019;10:12 pubmed publisher
Li X, Jia L, Chen X, Dong Y, Ren X, Dong Y, et al. Islet α-cell Inflammation Induced By NF-κB inducing kinase (NIK) Leads to Hypoglycemia, Pancreatitis, Growth Retardation, and Postnatal Death in Mice. Theranostics. 2018;8:5960-5971 pubmed publisher
Bai J, Gao Y, Chen L, Yin Q, Lou F, Wang Z, et al. Identification of a natural inhibitor of methionine adenosyltransferase 2A regulating one-carbon metabolism in keratinocytes. EBioMedicine. 2019;39:575-590 pubmed publisher
Izumi T, Imai J, Yamamoto J, Kawana Y, Endo A, Sugawara H, et al. Vagus-macrophage-hepatocyte link promotes post-injury liver regeneration and whole-body survival through hepatic FoxM1 activation. Nat Commun. 2018;9:5300 pubmed publisher
Morioka S, Perry J, Raymond M, Medina C, Zhu Y, Zhao L, et al. Efferocytosis induces a novel SLC program to promote glucose uptake and lactate release. Nature. 2018;563:714-718 pubmed publisher
Beneke V, Küster F, Neehus A, Hesse C, Lopez Rodriguez E, Haake K, et al. An immune cell spray (ICS) formulation allows for the delivery of functional monocyte/macrophages. Sci Rep. 2018;8:16281 pubmed publisher
Waterborg C, Koenders M, van Lent P, van der Kraan P, van de Loo F. Tyro3/Axl/Mertk-deficient mice develop bone marrow edema which is an early pathological marker in rheumatoid arthritis. PLoS ONE. 2018;13:e0205902 pubmed publisher
Feng Y, Liang Y, Zhu X, Wang M, Gui Y, Lu Q, et al. The signaling protein Wnt5a promotes TGFβ1-mediated macrophage polarization and kidney fibrosis by inducing the transcriptional regulators Yap/Taz. J Biol Chem. 2018;293:19290-19302 pubmed publisher
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product information
Product Type :
Antibody
Product Name :
F4/80 Monoclonal Antibody (BM8), Alexa Fluor™ 488, eBioscience
Catalog # :
53-4801-82
Quantity :
100 µg
Price :
US 356
Clonality :
Monoclonal
Purity :
Affinity chromatography
Host :
Rat
Reactivity :
Mouse
Applications :
Flow Cytometry: 0.5 µg/test
Species :
Mouse
Clone :
BM8
Isotype :
IgG2a, kappa
Storage :
4° C, store in dark, DO NOT FREEZE!
Description :
F4/80 antigen is a 160 kDa cell surface glycoprotein that is a member of the EGF TM7 family of proteins and shares 68% overall amino acid identity with human EGF module containing mucin like hormone receptor 1 (EMR1). Expression of F4/80 is heterogeneous and is reported to vary during macrophage maturation and activation. The F4/80 antigen is expressed on a wide range of mature tissue macrophages including Kupffer cells, Langerhans, microglia, macrophages located in the gut lamina propria, peritoneal cavity, lung, thymus, bone marrow stroma and macrophages in the red pulp of the spleen. F4/80 expression has also been reported on a subpopulation of dendritic cells but is absent from macrophages located in T cell areas of the spleen and lymph node. The ligands and biological functions of the F4/80 antigen have not yet been determined but recent studies suggest a role for F4/80 in the generation of efferent CD8+ve regulatory T cells.
Format :
Liquid
Applications w/Dilutions :
Flow Cytometry: 0.5 µg/test
Aliases :
ADGRE1; Adhesion G protein-coupled receptor E1; Adhesion G protein-coupled receptor E2; cell surface glycoprotein EMR1; cell surface glycoprotein F4/80; DD7A5-7; EGF-like module containing, mucin-like, hormone receptor-like 1; EGF-like module containing, mucin-like, hormone receptor-like sequence 1; EGF-like module receptor 1; EGF-like module-containing mucin-like hormone receptor-like 1; EGF-TM7; Emr1; EMR1 hormone receptor; F4/80; Gpf480; Ly71; lymphocyte antigen 71; TM7LN3
more info or order :
company information
Invitrogen
Thermo Fisher Scientific
81 Wyman Street
Waltham, MA USA 02451
https://www.thermofisher.com
800-678-5599
headquarters: USA