FOXP3 Monoclonal Antibody (236A/E7), Alexa Fluor™ 488, eBioscience | Invitrogen 53-4777-41 product information

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product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

FOXP3 Monoclonal Antibody (236A/E7), Alexa Fluor™ 488, eBioscience

catalog :

53-4777-41

quantity :

25 Tests

price :

US 186

clonality :

monoclonal

host :

mouse

conjugate :

AF488

clone name :

236a/E7

reactivity :

human, rhesus macaque

application :

immunohistochemistry, flow cytometry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

citations: 48

Published Application/Species/Sample/Dilution	Reference
flow cytometry; human; loading ...; fig 1a	Goldstein J, Burlion A, Zaragoza B, Sendeyo K, Polansky J, Huehn J, et al. Inhibition of the JAK/STAT Signaling Pathway in Regulatory T Cells Reveals a Very Dynamic Regulation of Foxp3 Expression. PLoS ONE. 2016;11:e0153682 pubmed publisher
	von Schoenfeld A, Bronsert P, Poc M, Fuller A, Filby A, Kraft S, et al. Multiple Immunostainings with Different Epitope Retrievals-The FOLGAS Protocol. Int J Mol Sci. 2021;23: pubmed publisher
	Barbosa A, Martinho O, Nogueira R, Campos J, Lobo L, Pinto H, et al. Increased CD3+, CD8+, or FoxP3+ T Lymphocyte Infiltrations Are Associated with the Pathogenesis of Colorectal Cancer but Not with the Overall Survival of Patients. Biology (Basel). 2021;10: pubmed publisher
	Sacrist xe1 n G xf3 mez P, Serrano Somavilla A, Gonz xe1 lez Amaro R, Mart xed nez Hern xe1 ndez R, Marazuela M. Analysis of Expression of Different Histone Deacetylases in Autoimmune Thyroid Disease. J Clin Endocrinol Metab. 2021;106:3213-3227 pubmed publisher
	Xydia M, Rahbari R, Ruggiero E, Macaulay I, Tarabichi M, Lohmayer R, et al. Common clonal origin of conventional T cells and induced regulatory T cells in breast cancer patients. Nat Commun. 2021;12:1119 pubmed publisher
	Gómez Aleza C, Nguyen B, Yoldi G, Ciscar M, Barranco A, Hernandez Jimenez E, et al. Inhibition of RANK signaling in breast cancer induces an anti-tumor immune response orchestrated by CD8+ T cells. Nat Commun. 2020;11:6335 pubmed publisher
	Todorova D, Zhang Y, Chen Q, Liu J, He J, Fu X, et al. hESC-derived immune suppressive dendritic cells induce immune tolerance of parental hESC-derived allografts. EBioMedicine. 2020;62:103120 pubmed publisher
	Mi H, Gong C, Sulam J, Fertig E, Szalay A, Jaffee E, et al. Digital Pathology Analysis Quantifies Spatial Heterogeneity of CD3, CD4, CD8, CD20, and FoxP3 Immune Markers in Triple-Negative Breast Cancer. Front Physiol. 2020;11:583333 pubmed publisher
	Zhang M, Liu Y, Chen J, Chen L, Meng J, Yang C, et al. Single-cell multi-omics analysis presents the landscape of peripheral blood T-cell subsets in human chronic prostatitis/chronic pelvic pain syndrome. J Cell Mol Med. 2020;24:14099-14109 pubmed publisher
	Scherer A, Stephens V, McGivney G, Gutierrez W, Laverty E, Knepper Adrian V, et al. Distinct Tumor Microenvironments Are a Defining Feature of Strain-Specific CRISPR/Cas9-Induced MPNSTs. Genes (Basel). 2020;11: pubmed publisher
	Court A, Le Gatt A, Luz Crawford P, Parra E, Aliaga Tobar V, Batiz L, et al. Mitochondrial transfer from MSCs to T cells induces Treg differentiation and restricts inflammatory response. EMBO Rep. 2020;21:e48052 pubmed publisher
	Silva M, Triltsch N, Leis S, Kanchev I, Tan T, Van Peel B, et al. Biomarker recommendation for PD-1/PD-L1 immunotherapy development in pediatric cancer based on digital image analysis of PD-L1 and immune cells. J Pathol Clin Res. 2020;6:124-137 pubmed publisher
	Rashid R, Gaglia G, Chen Y, Lin J, Du Z, Maliga Z, et al. Highly multiplexed immunofluorescence images and single-cell data of immune markers in tonsil and lung cancer. Sci Data. 2019;6:323 pubmed publisher
	Triozzi P, Schoenfield L, Plesec T, Saunthararajah Y, Tubbs R, Singh A. Molecular profiling of primary uveal melanomas with tumor-infiltrating lymphocytes. Oncoimmunology. 2019;8:e947169 pubmed publisher
	Zhang Y, Du X, Liu M, Tang F, Zhang P, Ai C, et al. Hijacking antibody-induced CTLA-4 lysosomal degradation for safer and more effective cancer immunotherapy. Cell Res. 2019;29:609-627 pubmed publisher
	Roelofsen T, Wefers C, Gorris M, Textor J, Massuger L, de Vries I, et al. Spontaneous Regression of Ovarian Carcinoma After Septic Peritonitis; A Unique Case Report. Front Oncol. 2018;8:562 pubmed publisher
	Moreira D, Adamus T, Zhao X, Su Y, Zhang Z, White S, et al. STAT3 Inhibition Combined with CpG Immunostimulation Activates Antitumor Immunity to Eradicate Genetically Distinct Castration-Resistant Prostate Cancers. Clin Cancer Res. 2018;24:5948-5962 pubmed publisher
	Kelly D, Li X, Kilday C, Feldman S, Clark S, Liu F, et al. Increased circulating regulatory T cells in medicated people with schizophrenia. Psychiatry Res. 2018;269:517-523 pubmed publisher
	Onion D, Isherwood M, Shridhar N, Xenophontos M, Craze M, Day L, et al. Multicomponent analysis of the tumour microenvironment reveals low CD8 T cell number, low stromal caveolin-1 and high tenascin-C and their combination as significant prognostic markers in non-small cell lung cancer. Oncotarget. 2018;9:1760-1771 pubmed publisher
	Terranova Barberio M, Thomas S, Ali N, Pawlowska N, Park J, Krings G, et al. HDAC inhibition potentiates immunotherapy in triple negative breast cancer. Oncotarget. 2017;8:114156-114172 pubmed publisher
	Kishore M, Cheung K, Fu H, Bonacina F, Wang G, Coe D, et al. Regulatory T Cell Migration Is Dependent on Glucokinase-Mediated Glycolysis. Immunity. 2017;47:875-889.e10 pubmed publisher
	Jiang X, Seo Y, Chang J, Coveler A, Nigjeh E, Pan S, et al. Long-lived pancreatic ductal adenocarcinoma slice cultures enable precise study of the immune microenvironment. Oncoimmunology. 2017;6:e1333210 pubmed publisher
	Zanin Zhorov A, Weiss J, Trzeciak A, Chen W, Zhang J, Nyuydzefe M, et al. Cutting Edge: Selective Oral ROCK2 Inhibitor Reduces Clinical Scores in Patients with Psoriasis Vulgaris and Normalizes Skin Pathology via Concurrent Regulation of IL-17 and IL-10. J Immunol. 2017;198:3809-3814 pubmed publisher
	Syed Khaja A, Toor S, El Salhat H, Faour I, Ul Haq N, Ali B, et al. Preferential accumulation of regulatory T cells with highly immunosuppressive characteristics in breast tumor microenvironment. Oncotarget. 2017;8:33159-33171 pubmed publisher
	Su S, Liao J, Liu J, Huang D, He C, Chen F, et al. Blocking the recruitment of naive CD4+ T cells reverses immunosuppression in breast cancer. Cell Res. 2017;27:461-482 pubmed publisher
	Weingartner E, Courneya J, Keegan A, Golding A. A novel method for assaying human regulatory T cell direct suppression of B cell effector function. J Immunol Methods. 2017;441:1-7 pubmed publisher
	Kovacsovics Bankowski M, Kelley T, Efimova O, Kim S, Wilson A, Swierczek S, et al. Changes in peripheral blood lymphocytes in polycythemia vera and essential thrombocythemia patients treated with pegylated-interferon alpha and correlation with JAK2V617F allelic burden. Exp Hematol Oncol. 2015;5:28 pubmed
	Miyan M, Schmidt Mende J, Kiessling R, Poschke I, de Boniface J. Differential tumor infiltration by T-cells characterizes intrinsic molecular subtypes in breast cancer. J Transl Med. 2016;14:227 pubmed publisher
	Baras A, Drake C, Liu J, Gandhi N, Kates M, Hoque M, et al. The ratio of CD8 to Treg tumor-infiltrating lymphocytes is associated with response to cisplatin-based neoadjuvant chemotherapy in patients with muscle invasive urothelial carcinoma of the bladder. Oncoimmunology. 2016;5:e1134412 pubmed publisher
	Liang D, Hou Y, Luo L, Ao L. Altered expression of miR-92a correlates with Th17 cell frequency in patients with primary biliary cirrhosis. Int J Mol Med. 2016;38:131-8 pubmed publisher
	Ghosh S, Taylor A, Chin M, Huang H, Conery A, Mertz J, et al. Regulatory T Cell Modulation by CBP/EP300 Bromodomain Inhibition. J Biol Chem. 2016;291:13014-27 pubmed publisher
	Macdonald K, Hoeppli R, Huang Q, Gillies J, Luciani D, Orban P, et al. Alloantigen-specific regulatory T cells generated with a chimeric antigen receptor. J Clin Invest. 2016;126:1413-24 pubmed publisher
	Thompson E, Taube J, Elwood H, Sharma R, Meeker A, Warzecha H, et al. The immune microenvironment of breast ductal carcinoma in situ. Mod Pathol. 2016;29:249-58 pubmed publisher
	Miles B, Miller S, Folkvord J, Kimball A, Chamanian M, Meditz A, et al. Follicular regulatory T cells impair follicular T helper cells in HIV and SIV infection. Nat Commun. 2015;6:8608 pubmed publisher
	Skogberg G, Lundberg V, Lindgren S, Gudmundsdottir J, Sandström K, Kämpe O, et al. Altered expression of autoimmune regulator in infant down syndrome thymus, a possible contributor to an autoimmune phenotype. J Immunol. 2014;193:2187-95 pubmed publisher
	Ye S, Li Z, Luo D, Huang B, Chen Y, Zhang X, et al. Tumor-derived exosomes promote tumor progression and T-cell dysfunction through the regulation of enriched exosomal microRNAs in human nasopharyngeal carcinoma. Oncotarget. 2014;5:5439-52 pubmed
	Schmidt H, Ge Y, Hartmann F, Conrad H, Klug F, Nittel S, et al. HLA Class II tetramers reveal tissue-specific regulatory T cells that suppress T-cell responses in breast carcinoma patients. Oncoimmunology. 2013;2:e24962 pubmed
	Luopajarvi K, Nieminen J, Ilonen J, Akerblom H, Knip M, Vaarala O. Expansion of CD4+CD25+FOXP3+ regulatory T cells in infants of mothers with type 1 diabetes. Pediatr Diabetes. 2012;13:400-7 pubmed publisher
	Sucher R, Fischler K, Oberhuber R, Kronberger I, Margreiter C, Ollinger R, et al. IDO and regulatory T cell support are critical for cytotoxic T lymphocyte-associated Ag-4 Ig-mediated long-term solid organ allograft survival. J Immunol. 2012;188:37-46 pubmed publisher
	Green A, Mattila J, Bigbee C, Bongers K, Lin P, Flynn J. CD4(+) regulatory T cells in a cynomolgus macaque model of Mycobacterium tuberculosis infection. J Infect Dis. 2010;202:533-41 pubmed publisher
	Tran D, Andersson J, Hardwick D, Bebris L, Illei G, Shevach E. Selective expression of latency-associated peptide (LAP) and IL-1 receptor type I/II (CD121a/CD121b) on activated human FOXP3+ regulatory T cells allows for their purification from expansion cultures. Blood. 2009;113:5125-33 pubmed publisher
	Kasprzycka M, Zhang Q, Witkiewicz A, Marzec M, Potoczek M, Liu X, et al. Gamma c-signaling cytokines induce a regulatory T cell phenotype in malignant CD4+ T lymphocytes. J Immunol. 2008;181:2506-12 pubmed
	Estes J, Gordon S, Zeng M, Chahroudi A, Dunham R, Staprans S, et al. Early resolution of acute immune activation and induction of PD-1 in SIV-infected sooty mangabeys distinguishes nonpathogenic from pathogenic infection in rhesus macaques. J Immunol. 2008;180:6798-807 pubmed
	Wolf D, Wolf A, Rumpold H, Fiegl H, Zeimet A, Muller Holzner E, et al. The expression of the regulatory T cell-specific forkhead box transcription factor FoxP3 is associated with poor prognosis in ovarian cancer. Clin Cancer Res. 2005;11:8326-31 pubmed
	Ahmadzadeh M, Rosenberg S. IL-2 administration increases CD4+ CD25(hi) Foxp3+ regulatory T cells in cancer patients. Blood. 2006;107:2409-14 pubmed
	Roncador G, Garcia J, Maestre L, Lucas E, Menarguez J, Ohshima K, et al. FOXP3, a selective marker for a subset of adult T-cell leukaemia/lymphoma. Leukemia. 2005;19:2247-53 pubmed
	Roncador G, Brown P, Maestre L, Hue S, Martinez Torrecuadrada J, Ling K, et al. Analysis of FOXP3 protein expression in human CD4+CD25+ regulatory T cells at the single-cell level. Eur J Immunol. 2005;35:1681-91 pubmed
	Alvaro T, Lejeune M, Salvadó M, Bosch R, Garcia J, Jaén J, et al. Outcome in Hodgkin's lymphoma can be predicted from the presence of accompanying cytotoxic and regulatory T cells. Clin Cancer Res. 2005;11:1467-73 pubmed

product information

Product Type :

Antibody

Product Name :

FOXP3 Monoclonal Antibody (236A/E7), Alexa Fluor™ 488, eBioscience

Catalog # :

53-4777-41

Quantity :

25 Tests

Price :

US 186

Clonality :

Monoclonal

Purity :

Affinity chromatography

Host :

Mouse

Reactivity :

Human, Non-human primate, Rhesus Monkey

Applications :

Flow Cytometry: 5 µL (0.5 µg)/test, Immunohistochemistry (Frozen): Assay-Dependent, Immunohistochemistry (Paraffin): Assay-Dependent

Species :

Human, Non-human primate, Rhesus Monkey

Clone :

236A/E7

Isotype :

IgG1, kappa

Storage :

4° C, store in dark, DO NOT FREEZE!

Description :

FOXP3 (Forkhead box protein 3) is a member of the forkhead/winged-helix family of transcriptional regulators, highly conserved across mammals, and essential for normal immune homeostasis. FOXP3 is 381 amino acids long, stably and constitutively expressed at a high level in CD25 + CD4 positive regulatory T cells, a low level in CD4-positive/CD25-negative cells, and is absent in CD4-negative/CD8-positive T cells. FOXP3 may be a master regulatory gene, and a more specific marker of regulatory T cells. Defects in the gene encoding FOXP3 protein cause the scurfy phenotype in mice. In humans FOXP3 defects play a role in IPEX syndrome (immune dysfunction, polyendocrinopathy, enteropathy, X-linked syndrome), also known as X-linked autoimmunity-allergic dysregulation (XLAAD) syndrome. Transcript variants of FOXP3 encoding different isoforms have been identified. In human breast and colon cancer cells, expression of FOXP3 is regulated by p53 in response to the DNA damage.

Format :

Liquid

Applications w/Dilutions :

Flow Cytometry: 5 µL (0.5 µg)/test, Immunohistochemistry (Frozen): Assay-Dependent, Immunohistochemistry (Paraffin): Assay-Dependent

Aliases :

AIID; DIETER; forkhead box P3; forkhead box protein P3; Forkhead box protein P3 41 kDa form; Forkhead box protein P3, C-terminally processed; forkhead/winged helix transcription factor 3; Foxp3; FOXP3delta7; immune dysregulation, polyendocrinopathy, enteropathy, X-linked; immunodeficiency, polyendocrinopathy, enteropathy, X-linked; IPEX; JM2; MGC141961; MGC141963; PIDX; regulatory protein Foxp3; RGD1562112; RP23-54C14.1; scurfin; scurfy; sf; XPID