This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Invitrogen
other brands :
NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience
product type :
antibody
product name :
Staphylococcus aureus Cas9 Monoclonal Antibody (6H4), Alexa Fluor 647, eBioscience
catalog :
51-6499-82
quantity :
100 ug
price :
US 467.00
clonality :
monoclonal
host :
mouse
conjugate :
AF647
clone name :
6H4
application :
immunocytochemistry, flow cytometry
product information
Product Type :
Antibody
Product Name :
Staphylococcus aureus Cas9 Monoclonal Antibody (6H4), Alexa Fluor 647, eBioscience
Catalog # :
51-6499-82
Quantity :
100 ug
Price :
US 467.00
Clonality :
Monoclonal
Purity :
Affinity chromatography
Host :
Mouse
Reactivity :
Bacteria
Applications :
Flow Cytometry: 0.06 ug/test, Immunocytochemistry: 2.5 ug/ml
Species :
Bacteria
Clone :
6H4
Isotype :
IgG2b, kappa
Storage :
4 C, store in dark, DO NOT FREEZE!
Description :
Cas9 is an RNA-guided DNA endonuclease enzyme associated with the CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) adaptive immunity system in bacteria and genetic engineering. The system is unique and flexible due to its dependence on RNA as the element that targets the nuclease to a desired DNA sequence. It can be used to induce indel mutations, specific sequence replacements/insertions, and large deletions or genomic rearrangements at any desired location in the genome. In addition, Cas9 can also be used to mediate upregulation of specific endogenous genes or to alter histone modifications or DNA methylation. Cas9 may be derived from Streptococcus pyogenes (SpCas9) or Staphylococcus aureus (SaCas9). SaCas9 has a number of properties that make it advantageous for genome editing, including its small size, high efficiency, nickase activity, and apparent specificity. SaCas9 principally recognizes an NNGRRT protospacer adjacent motif (PAM) and cleaves target DNA at rates comparable to SpCas9 with an ideal spacer lengths of 24 to 20 nucleotides. In comparison to SpCas9, SaCas9 is smaller (1368 AA versus 1053 AA) and generates indels at a comparable rate when directed against target DNA with the mutually permissive NGGRRT PAM. This makes it the preferred enzyme for all-in-one delivery of Cas9 and multiple gRNA expression cassettes with Adeno-associated vectors.
Immunogen :
His-tagged recombinant Cas-9 from Staphylococcus aureus (maps to N-terminus)
Format :
Liquid
Applications w/Dilutions :
Flow Cytometry: 0.06 ug/test, Immunocytochemistry: 2.5 ug/ml
Aliases :
cas9; CRISPR9; CRISPR-Cas9; s aureus
company information
Invitrogen
Thermo Fisher Scientific
81 Wyman Street
Waltham, MA USA 02451
https://www.thermofisher.com
800-678-5599
headquarters: USA