product summary
Loading...
company name :
Invitrogen
other brands :
NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience
product type :
antibody
product name :
LAMP-2A Polyclonal Antibody (AMC2)
catalog :
51-2200
quantity :
100 µg
price :
375 USD
clonality :
polyclonal
host :
domestic rabbit
conjugate :
nonconjugated
clone name :
AMC2
reactivity :
mouse, rat
application :
western blot, ELISA, immunohistochemistry - paraffin section
more info or order :
Invitrogen product webpage
citations: 23
Published Application/Species/Sample/DilutionReference
  • immunohistochemistry - paraffin section; rat; 1:100; loading ...; fig st10
  • immunohistochemistry - paraffin section; mouse; 1:1000; loading ...; fig st10
Furukawa S, Nagaike M, Ozaki K. Databases for technical aspects of immunohistochemistry. J Toxicol Pathol. 2017;30:79-107 pubmed publisher
  • western blot; mouse; 1:4000; loading ...; fig 3a
Vodicka P, Chase K, Iuliano M, Tousley A, Valentine D, Sapp E, et al. Autophagy Activation by Transcription Factor EB (TFEB) in Striatum of HDQ175/Q7 Mice. J Huntingtons Dis. 2016;5:249-260 pubmed
  • western blot; mouse; 1:1000; fig 6
Basisty N, Dai D, Gagnidze A, Gitari L, Fredrickson J, Maina Y, et al. Mitochondrial-targeted catalase is good for the old mouse proteome, but not for the young: 'reverse' antagonistic pleiotropy?. Aging Cell. 2016;15:634-45 pubmed publisher
  • immunohistochemistry - paraffin section; mouse; 1:200; fig s6
Shimshek D, Jacobson L, Kolly C, Zamurovic N, Balavenkatraman K, Morawiec L, et al. Pharmacological BACE1 and BACE2 inhibition induces hair depigmentation by inhibiting PMEL17 processing in mice. Sci Rep. 2016;6:21917 pubmed publisher
Lo Dico A, Salvatore D, Martelli C, Ronchi D, Diceglie C, Lucignani G, et al. Intracellular Redox-Balance Involvement in Temozolomide Resistance-Related Molecular Mechanisms in Glioblastoma. Cells. 2019;8: pubmed publisher
Kirchner P, Bourdenx M, Madrigal Matute J, Tiano S, Díaz A, Bartholdy B, et al. Proteome-wide analysis of chaperone-mediated autophagy targeting motifs. PLoS Biol. 2019;17:e3000301 pubmed publisher
Wu Z, Geng Y, Lu X, Shi Y, Wu G, Zhang M, et al. Chaperone-mediated autophagy is involved in the execution of ferroptosis. Proc Natl Acad Sci U S A. 2019;116:2996-3005 pubmed publisher
Lee S, Shin S, Zhong P, Kim H, Kim D, Kim J, et al. Reciprocal control of excitatory synapse numbers by Wnt and Wnt inhibitor PRR7 secreted on exosomes. Nat Commun. 2018;9:3434 pubmed publisher
Trachsel Moncho L, Benlloch Navarro S, Fernández Carbonell Á, Ramírez Lamelas D, Olivar T, Silvestre D, et al. Oxidative stress and autophagy-related changes during retinal degeneration and development. Cell Death Dis. 2018;9:812 pubmed publisher
Sakurai T, Kamio K, Sasaki K, Nishimoto T, Yamaguchi J, Sasaki M, et al. Imaging Mass Microscopy of Kidneys from Azithromycin-Treated Rats with Phospholipidosis. Am J Pathol. 2018;188:1993-2003 pubmed publisher
Ma J, Snook S, Garrovillo S, Johnson C, La D. An Immunohistochemical Investigation of Renal Phospholipidosis and Toxicity in Rats. Int J Toxicol. 2017;36:386-394 pubmed publisher
Kim Y, Hood D. Regulation of the autophagy system during chronic contractile activity-induced muscle adaptations. Physiol Rep. 2017;5: pubmed publisher
Zhang J, Johnson J, He J, Napolitano G, Ramadass M, Rocca C, et al. Cystinosin, the small GTPase Rab11, and the Rab7 effector RILP regulate intracellular trafficking of the chaperone-mediated autophagy receptor LAMP2A. J Biol Chem. 2017;292:10328-10346 pubmed publisher
Into T, Horie T, Inomata M, Gohda J, Inoue J, Murakami Y, et al. Basal autophagy prevents autoactivation or enhancement of inflammatory signals by targeting monomeric MyD88. Sci Rep. 2017;7:1009 pubmed publisher
Gomes L, Menck C, Cuervo A. Chaperone-mediated autophagy prevents cellular transformation by regulating MYC proteasomal degradation. Autophagy. 2017;13:928-940 pubmed publisher
Braendli Baiocco A, Festag M, Dumong Erichsen K, Persson R, Mihatsch M, Fisker N, et al. From the Cover: The Minipig is a Suitable Non-Rodent Model in the Safety Assessment of Single Stranded Oligonucleotides. Toxicol Sci. 2017;157:112-128 pubmed publisher
Xilouri M, Brekk O, Polissidis A, Chrysanthou Piterou M, Kloukina I, Stefanis L. Impairment of chaperone-mediated autophagy induces dopaminergic neurodegeneration in rats. Autophagy. 2016;12:2230-2247 pubmed
Mitchell S, Madrigal Matute J, Scheibye Knudsen M, Fang E, Aon M, González Reyes J, et al. Effects of Sex, Strain, and Energy Intake on Hallmarks of Aging in Mice. Cell Metab. 2016;23:1093-1112 pubmed publisher
Kaushik S, Cuervo A. AMPK-dependent phosphorylation of lipid droplet protein PLIN2 triggers its degradation by CMA. Autophagy. 2016;12:432-8 pubmed publisher
Mareninova O, Sendler M, Malla S, Yakubov I, French S, Tokhtaeva E, et al. Lysosome associated membrane proteins maintain pancreatic acinar cell homeostasis: LAMP-2 deficient mice develop pancreatitis. Cell Mol Gastroenterol Hepatol. 2015;1:678-694 pubmed
Kaushik S, Cuervo A. Degradation of lipid droplet-associated proteins by chaperone-mediated autophagy facilitates lipolysis. Nat Cell Biol. 2015;17:759-70 pubmed publisher
Park Y, Liu C, Luo T, Dietrich W, Bramlett H, Hu B. Chaperone-Mediated Autophagy after Traumatic Brain Injury. J Neurotrauma. 2015;32:1449-57 pubmed publisher
Kett L, Stiller B, Bernath M, Tasset I, Blesa J, Jackson Lewis V, et al. α-Synuclein-independent histopathological and motor deficits in mice lacking the endolysosomal Parkinsonism protein Atp13a2. J Neurosci. 2015;35:5724-42 pubmed publisher
product information
Product Type :
Antibody
Product Name :
LAMP-2A Polyclonal Antibody (AMC2)
Catalog # :
51-2200
Quantity :
100 µg
Price :
375 USD
Clonality :
Polyclonal
Purity :
Antigen affinity chromatography
Host :
Rabbit
Reactivity :
Mouse, Rat
Applications :
ELISA: 0.1-1 µg/mL, Inhibition Assays: Assay Dependent, Western Blot: 0.1-1 µg/mL
Species :
Mouse, Rat
Clone :
AMC2
Isotype :
IgG
Storage :
-20°C
Description :
LAMP2 (Lysosome-associated membrane glycoprotein 2, CD107b) is a member of a family of membrane glycoproteins. LAMP2 provides selectins with carbohydrate ligands, and may play a role in tumor cell metastasis, and have a function in the protection, maintenance, and adhesion of the lysosome. Prior to posttranslational modification, LAMP2 is approximately 46 kDa polypeptide. Mature, functional LAMP2 is extensively glycosylated with a variety of different N-linked and O-linked oligosaccharides with a total molecular weight of about 100-130 kDa. LAMP 2 has a structure comprised of a large amino-terminal intra-lysosomal domain, a hydrophobic trans-membrane domain, and a short carboxyl-terminal cytoplasmic tail. LAMP2 is used to monitor a very selective form of autophagy called chaperone-mediated autophagy. LAMP 2 has a structure comprised of a large amino-terminal intra-lysosomal domain, a hydrophobic trans-membrane domain, and a short carboxyl-terminal cytoplasmic tail. The LAMP protein has 3 variant forms: LAMP-2A, -2B, and 2C. LAMP2, together with CD107a/LAMP-1, is a major constituent of lysosomal membrane. The LAMP proteins are involved in lysosome biogenesis and are required for fusion of lysosomes with phagosomes, and LAMP2 is an important regulator in successful phagosomal maturation. LAMP2 deficiency causes an accumulation of autophagosomes in many tissues leading to cardiomyopathy and myopathy (Danon’s disease).
Immunogen :
A synthetic peptide of the cytosolic tail of rat LAMP-2A (GLKRHHTGYEQF)
Format :
Liquid
Applications w/Dilutions :
ELISA: 0.1-1 µg/mL, Inhibition Assays: Assay Dependent, Western Blot: 0.1-1 µg/mL
Aliases :
CD107 antigen-like family member B; CD107b; EGK_20887; Igp96; Lamp II; LAMP2; Lamp-2; LAMP-2 Isoform 2A; LAMP-2 Variant A; Lamp-2a; Lamp-2b; Lamp-2c; LAMPB; LGP110; LGP-110; LGP-96; LGP-B; lysosomal associated membrane protein 2; lysosomal membrane glycoprotein 2; lysosomal membrane glycoprotein type B; lysosomal-associated membrane protein 2; lysosome-associated membrane glycoprotein 2; lysosome-associated membrane protein 2; Mac3; RP23-193O17.2
more info or order :
Invitrogen product webpage
company information
Invitrogen
Thermo Fisher Scientific
81 Wyman Street
Waltham, MA USA 02451
https://www.thermofisher.com
800-678-5599
headquarters: USA