Invitrogen ACO2 antibody

Aconitase 2 Polyclonal Antibody
Invitrogen
catalog: PA5-19269

domestic goat polyclonal
reactivity: human, mouse, rat, pigs
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section
citations: 1

Western Blot staining of Human Heart lysate using PA5-19269 at a concentration of 0.01 ug/ml, the primary antibody incubation was 1 hour and the detection method was chemiluminescence.
quantity: 100 ug
price: US 464.00
to the supplier

Aconitase 2 Monoclonal Antibody (7G4)
Invitrogen
catalog: MA1-029

mouse monoclonal (7G4)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

Immunofluorescent analysis of Aco-2 using anti-Aco-2 monoclonal antibody (Product# MA1-029) (shown in green) in HeLa cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature. Cells were then blocked with 1% Blocker BSA (Product #37525) for 15 minutes at room temperature. Cells were probed with a mouse monoclonal antibody recognizing Aco-2 (Product# MA1-029), at a dilution of 1:100 for at least 1 hour at room temperature. Cells were then washed with PBS and incubated with DyLight 488 goat-anti-mouse secondary antibody (Product# 35503) at a dilution of 1:400 for 30 minutes at room temperature. Nuclei (blue) were stained with Hoechst 33342 dye (Product# 62249). Images were taken on a Thermo Scientific ArrayScan at 20X magnification.

Immunohistochemistry was performed on cancer biopsies of deparaffinized human colon, myocardial, hepatic and renal cell carcinoma tissues. To expose target proteins heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer for 20 minutes at 95°C. Following antigen retrieval tissues were blocked in 10% normal goat serum (Product #31873) for 20 minutes at room temperature. Tissues were then probed at a dilution of 1:800 with a mouse monoclonal antibody recognizing Aco-2 (Product # MA1-029) overnight at 4°C in a humidified chamber. Detection was performed using a goat anti-mouse HRP secondary antibody followed by colorimetric detection using DAB substrate. Tissues were counterstained with hematoxylin and prepped for mounting. Images were taken at 400X magnification. Results demonstrate cytoplasmic localization of mitochondrial aconitase 2.

Western blot analysis of Aco-2 was performed by loading 25ug of whole cell lysate from Aco-2 SMART pool siRNA (Product# L-009566-01-0005) transfected or non-targeting control (Product# D-001810-10) transfected MCF7 cells onto a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked with 5% Milk/TBST for at least 1 hour. Membranes were probed with Aco-2 mouse monoclonal antibody (Product # MA1-029) at a dilution of 1:1000 overnight at 4°C on a rocking platform. Membranes were washed in TBS-0.1%Tween 20 and probed with a goat anti-mouse-HRP secondary antibody (Product # 31430) at a dilution of 1:20,000 for at least one hour. Membranes were washed and chemiluminescent detection performed using Super Signal West Dura (Product #34075).
quantity: 100 ug
price: US 491.00
to the supplier

Aconitase 2 Polyclonal Antibody
Invitrogen
catalog: PA5-19268

domestic goat polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

Western Blot staining of Human Heart lysate using PA5-19268 at a concentration of 0.01 ug/ml, the primary antibody incubation was 1 hour and the detection method was chemiluminescence.
quantity: 100 ug
price: US 464.00
to the supplier

Aconitase 2 Monoclonal Antibody (7G4)
Invitrogen
catalog: MA1-029X

mouse monoclonal (7G4)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

Immunohistochemistry was performed on cancer biopsies of deparaffinized human colon, myocardial, hepatic and renal cell carcinoma tissues. To expose target proteins heat induced antigen retrieval was performed using 10 mM sodium citrate (pH6.0) buffer for 20 minutes at 95C. Following antigen retrieval tissues were blocked in 10% normal goat serum (Product #31873) for 20 minutes at room temperature. Tissues were then probed at a dilution of 1:800 with a mouse monoclonal antibody recognizing Aco-2 (Product # MA1-029) overnight at 4C in a humidified chamber. Detection was performed using a goat anti-mouse HRP secondary antibody followed by colorimetric detection using DAB substrate. Tissues were counterstained with hematoxylin and prepped for mounting. Images were taken at 400X magnification. Results demonstrate cytoplasmic localization of mitochondrial aconitase 2.

Western blot analysis of Aco-2 was performed by loading 25ug of various whole cell lysates onto a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked with 5% Milk/TBST for at least 1 hour. Membranes were probed with a mouse monoclonal antibody recognizing Aco-2 (Product # MA1-029) at a dilution of 1:1000 overnight at 4°C on a rocking platform. Membranes were washed in TBS-0.1% Tween 20 and probed with a goat anti-mouse-HRP secondary antibody (Product # 31430) at a dilution of 1:20,000 for at least one hour. Membranes were washed and chemiluminescent detection performed using Super Signal West Dura (Product #34075).
quantity: 20 uL
price: US 187.00
to the supplier

Aconitase 2 Polyclonal Antibody
Invitrogen
catalog: PA5-29037

domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunohistochemistry - paraffin section

Confocal immunofluorescence analysis of methanol-fixed HeLa using Aconitase 2 (PA5-29037) antibody at 1:500 dilution.

Immunohistochemical analysis of paraffin-embedded HT29 xenograft using Aconitase 2 (PA5-29037) antibody at 1:500 dilution.

Western Blot analysis using anti-Aconitase 2 Polyclonal Antibody on the following cells: (30 ug of whole cell lysate) A: 293T B: A431 cell lysate. Primary antibody (PA5-29037) diluted at 1:10000
quantity: 100 uL
price: US 464.00
to the supplier

Aconitase 2 Polyclonal Antibody
Invitrogen
catalog: PA5-29960

domestic rabbit polyclonal
reactivity: human, mouse, rat, chicken
application: western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunohistochemistry - paraffin section

Immunofluorescence analysis of methanol-fixed A431 cells using Aconitase2 (PA5-29960) antibody at 1:200 dilution.

Immunohistochemical analysis of paraffin-embedded MCF7 xenograft using Aconitase2 (PA5-29960) antibody at 1:250 dilution.

Western Blot analysis of Aconitase 2 using anti-Aconitase 2 Polyclonal Antibody (PA5-29960), in Jurkat Cell Lysate (30 ug of cell lysate) and at a dilution of 1:1000.
quantity: 100 uL
price: US 464.00
to the supplier

Aconitase 2 Polyclonal Antibody
Invitrogen
catalog: PA5-80299

domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA, immunohistochemistry, immunoprecipitation, immunohistochemistry - paraffin section

quantity: 100 uL
price: US 319.00
to the supplier

Aconitase 2 Polyclonal Antibody
Invitrogen
catalog: PA5-81920

domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

quantity: 100 uL
price: US 522.00
to the supplier

Aconitase 2 Polyclonal Antibody
Invitrogen
catalog: PA5-86968

domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunohistochemistry - paraffin section

quantity: 100 uL
price: US 481.00
to the supplier

Aconitase 2 Polyclonal Antibody
Invitrogen
catalog: PA5-96252

domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, ELISA, immunohistochemistry, immunohistochemistry - paraffin section

quantity: 100 uL
price: US 464.00
to the supplier

Aconitase 2 Recombinant Rabbit Monoclonal Antibody (JE40-83)
Invitrogen
catalog: MA5-34820

domestic rabbit recombinant (JE40-83)
reactivity: human, mouse
application: western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunohistochemistry - paraffin section

quantity: 100 uL
price: US 474.00
to the supplier

Aconitase 2 Recombinant Rabbit Monoclonal Antibody (ARC1072)
Invitrogen
catalog: MA5-35527

domestic rabbit recombinant (ARC1072)
reactivity: human, mouse, rat
application: western blot, ELISA, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

quantity: 100 uL
price: US 464.00
to the supplier

Aconitase 2 Recombinant Rabbit Monoclonal Antibody (8H4)
Invitrogen
catalog: MA5-49928

domestic rabbit recombinant (8H4)
reactivity: human
application: ELISA, immunohistochemistry, immunohistochemistry - paraffin section

quantity: 100 uL
price: US 414.00
to the supplier

Aconitase 2 Polyclonal Antibody
Invitrogen
catalog: PA5-114812

domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, immunohistochemistry

quantity: 100 uL
price: US 464.00
to the supplier

Aconitase 2 Polyclonal Antibody
Invitrogen
catalog: PA5-142548

domestic goat polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry

quantity: 100 ug
price: US 444.00
to the supplier

Aconitase 2 Polyclonal Antibody, Biotin
Invitrogen
catalog: PA5-142549

domestic goat polyclonal
reactivity: human, mouse, rat, pigs
conjugate: biotin
application: western blot

quantity: 100 ug
price: US 444.00
to the supplier