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company name :
Invitrogen
other brands :
NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience
product type :
antibody
product name :
Gata-3 Monoclonal Antibody (TWAJ), eFluor 660, eBioscience
catalog :
50-9966-41
quantity :
25 Tests
price :
US 150
clonality :
monoclonal
host :
rat
conjugate :
efluor 660
clone name :
TWAJ
reactivity :
human, mouse, pigs , rhesus macaque
application :
flow cytometry
more info or order :
citations: 64
Published Application/Species/Sample/DilutionReference
  • flow cytometry; mouse; 1:100; fig s15e
Kimura S, Nakamura Y, Kobayashi N, Shiroguchi K, Kawakami E, Mutoh M, et al. Osteoprotegerin-dependent M cell self-regulation balances gut infection and immunity. Nat Commun. 2020;11:234 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 4b
Miyazaki M, Miyazaki K, Chen K, Jin Y, Turner J, Moore A, et al. The E-Id Protein Axis Specifies Adaptive Lymphoid Cell Identity and Suppresses Thymic Innate Lymphoid Cell Development. Immunity. 2017;46:818-834.e4 pubmed publisher
  • flow cytometry; mouse
Lu K, Keppler S, Leithäuser F, Mattfeldt T, Castello A, Kostezka U, et al. Nck adaptor proteins modulate differentiation and effector function of T cells. J Leukoc Biol. 2015;98:301-11 pubmed publisher
  • flow cytometry; mouse
Boyoglu Barnum S, Chirkova T, Todd S, Barnum T, Gaston K, Jorquera P, et al. Prophylaxis with a respiratory syncytial virus (RSV) anti-G protein monoclonal antibody shifts the adaptive immune response to RSV rA2-line19F infection from Th2 to Th1 in BALB/c mice. J Virol. 2014;88:10569-83 pubmed publisher
Sanchez Ferras O, Pacis A, Sotiropoulou M, Zhang Y, Wang Y, Bourgey M, et al. A coordinated progression of progenitor cell states initiates urinary tract development. Nat Commun. 2021;12:2627 pubmed publisher
Zu J, Yao L, Song Y, Cui Y, Guan M, Chen R, et al. Th2 Biased Immunity With Altered B Cell Profiles in Circulation of Patients With Sporotrichosis Caused by Sporothrix globosa. Front Immunol. 2020;11:570888 pubmed publisher
Rao T, Kumar S, Pulikkottil A, Oliveri F, Hendriks R, Beckel F, et al. Novel, Non-Gene-Destructive Knock-In Reporter Mice Refute the Concept of Monoallelic Gata3 Expression. J Immunol. 2020;204:2600-2611 pubmed publisher
Wiesner D, Merkhofer R, Ober C, Kujoth G, Niu M, Keller N, et al. Club Cell TRPV4 Serves as a Damage Sensor Driving Lung Allergic Inflammation. Cell Host Microbe. 2020;27:614-628.e6 pubmed publisher
Sidwell T, Liao Y, Garnham A, Vasanthakumar A, Gloury R, Blume J, et al. Attenuation of TCR-induced transcription by Bach2 controls regulatory T cell differentiation and homeostasis. Nat Commun. 2020;11:252 pubmed publisher
Dobson H, Dias L, Kohn E, Fites S, Wiesner D, Dileepan T, et al. Antigen discovery unveils resident memory and migratory cell roles in antifungal resistance. Mucosal Immunol. 2020;13:518-529 pubmed publisher
Huang A, Shinde P, Huang J, Senff T, Xu H, Margotta C, et al. Progranulin prevents regulatory NK cell cytotoxicity against antiviral T cells. JCI Insight. 2019;4: pubmed publisher
Jun J, Kulhanek K, Chen H, Chakraborty A, Roose J. Alternative ZAP70-p38 signals prime a classical p38 pathway through LAT and SOS to support regulatory T cell differentiation. Sci Signal. 2019;12: pubmed publisher
Eissmann M, Dijkstra C, Jarnicki A, Phesse T, Brunnberg J, Poh A, et al. IL-33-mediated mast cell activation promotes gastric cancer through macrophage mobilization. Nat Commun. 2019;10:2735 pubmed publisher
Esterházy D, Canesso M, Mesin L, Muller P, de Castro T, Lockhart A, et al. Compartmentalized gut lymph node drainage dictates adaptive immune responses. Nature. 2019;569:126-130 pubmed publisher
Dutton E, Camelo A, Sleeman M, Herbst R, Carlesso G, Belz G, et al. Characterisation of innate lymphoid cell populations at different sites in mice with defective T cell immunity. Wellcome Open Res. 2017;2:117 pubmed publisher
Li N, van Unen V, Höllt T, Thompson A, van Bergen J, Pezzotti N, et al. Mass cytometry reveals innate lymphoid cell differentiation pathways in the human fetal intestine. J Exp Med. 2018;215:1383-1396 pubmed publisher
Dalmas E, Lehmann F, Dror E, Wueest S, Thienel C, Borsigova M, et al. Interleukin-33-Activated Islet-Resident Innate Lymphoid Cells Promote Insulin Secretion through Myeloid Cell Retinoic Acid Production. Immunity. 2017;47:928-942.e7 pubmed publisher
Ahmed N, French T, Rausch S, Kühl A, Hemminger K, Dunay I, et al. Toxoplasma Co-infection Prevents Th2 Differentiation and Leads to a Helminth-Specific Th1 Response. Front Cell Infect Microbiol. 2017;7:341 pubmed publisher
Schwartz C, Khan A, Floudas A, Saunders S, Hams E, Rodewald H, et al. ILC2s regulate adaptive Th2 cell functions via PD-L1 checkpoint control. J Exp Med. 2017;214:2507-2521 pubmed publisher
Levine A, Mendoza A, Hemmers S, Moltedo B, Niec R, Schizas M, et al. Stability and function of regulatory T cells expressing the transcription factor T-bet. Nature. 2017;546:421-425 pubmed publisher
Lechner A, Driver I, Lee J, Conroy C, Nagle A, Locksley R, et al. Recruited Monocytes and Type 2 Immunity Promote Lung Regeneration following Pneumonectomy. Cell Stem Cell. 2017;21:120-134.e7 pubmed publisher
Campos Carrascosa L, Klein M, Kitagawa Y, Lückel C, Marini F, König A, et al. Reciprocal regulation of the Il9 locus by counteracting activities of transcription factors IRF1 and IRF4. Nat Commun. 2017;8:15366 pubmed publisher
Chen P, Hsieh M, Kuo W, Kao H, Hsu C, Wang J. Water-soluble chitosan inhibits nerve growth factor and attenuates allergic inflammation in mite allergen-induced allergic rhinitis. J Allergy Clin Immunol. 2017;140:1146-1149.e8 pubmed publisher
Iwata A, Durai V, Tussiwand R, Briseño C, Wu X, Grajales Reyes G, et al. Quality of TCR signaling determined by differential affinities of enhancers for the composite BATF-IRF4 transcription factor complex. Nat Immunol. 2017;18:563-572 pubmed publisher
Steinfelder S, Rausch S, Michael D, Kuhl A, Hartmann S. Intestinal helminth infection induces highly functional resident memory CD4+ T cells in mice. Eur J Immunol. 2017;47:353-363 pubmed publisher
Strandmark J, Steinfelder S, Berek C, Kühl A, Rausch S, Hartmann S. Eosinophils are required to suppress Th2 responses in Peyer's patches during intestinal infection by nematodes. Mucosal Immunol. 2017;10:661-672 pubmed publisher
Long C, Marshall N, Lukomska E, Kashon M, Meade B, Shane H, et al. A Role for Regulatory T Cells in a Murine Model of Epicutaneous Toluene Diisocyanate Sensitization. Toxicol Sci. 2016;152:85-98 pubmed publisher
Maaz D, Rausch S, Richter D, Krücken J, Kühl A, Demeler J, et al. Susceptibility to Ticks and Lyme Disease Spirochetes Is Not Affected in Mice Coinfected with Nematodes. Infect Immun. 2016;84:1274-1286 pubmed publisher
Halim T, Hwang Y, Scanlon S, Zaghouani H, Garbi N, Fallon P, et al. Group 2 innate lymphoid cells license dendritic cells to potentiate memory TH2 cell responses. Nat Immunol. 2016;17:57-64 pubmed publisher
Deng Y, Yang Y, Wang S, Li F, Liu M, Hua Q, et al. Intranasal Administration of Lentiviral miR-135a Regulates Mast Cell and Allergen-Induced Inflammation by Targeting GATA-3. PLoS ONE. 2015;10:e0139322 pubmed publisher
Marquardt N, Béziat V, Nyström S, Hengst J, Ivarsson M, Kekäläinen E, et al. Cutting edge: identification and characterization of human intrahepatic CD49a+ NK cells. J Immunol. 2015;194:2467-71 pubmed publisher
Uchiyama M, Jin X, Matsuda H, Bashuda H, Imazuru T, Shimokawa T, et al. An agonistic anti-BTLA mAb (3C10) induced generation of IL-10-dependent regulatory CD4+ T cells and prolongation of murine cardiac allograft. Transplantation. 2014;97:301-9 pubmed publisher
Villegas Mendez A, Gwyer Findlay E, de Souza J, Grady L, Saris C, Lane T, et al. WSX-1 signalling inhibits CD4? T cell migration to the liver during malaria infection by repressing chemokine-independent pathways. PLoS ONE. 2013;8:e78486 pubmed publisher
Hu T, Wang H, Simmons A, Bajana S, Zhao Y, Kovats S, et al. Increased level of E protein activity during invariant NKT development promotes differentiation of invariant NKT2 and invariant NKT17 subsets. J Immunol. 2013;191:5065-73 pubmed publisher
KEMP K, Lin Z, Zhao F, Gao B, Song J, Zhang K, et al. The serine-threonine kinase inositol-requiring enzyme 1? (IRE1?) promotes IL-4 production in T helper cells. J Biol Chem. 2013;288:33272-82 pubmed publisher
Mielke L, Groom J, Rankin L, Seillet C, Masson F, Putoczki T, et al. TCF-1 controls ILC2 and NKp46+ROR?t+ innate lymphocyte differentiation and protection in intestinal inflammation. J Immunol. 2013;191:4383-91 pubmed publisher
Blom L, Poulsen L. In vitro Th1 and Th2 cell polarization is severely influenced by the initial ratio of naïve and memory CD4+ T cells. J Immunol Methods. 2013;397:55-60 pubmed publisher
Durant L, Makris S, Voorburg C, Loebbermann J, Johansson C, Openshaw P. Regulatory T cells prevent Th2 immune responses and pulmonary eosinophilia during respiratory syncytial virus infection in mice. J Virol. 2013;87:10946-54 pubmed publisher
Chen Q, Yan L, Wang C, Wang W, Shi H, Su B, et al. Mesenchymal stem cells alleviate TNBS-induced colitis by modulating inflammatory and autoimmune responses. World J Gastroenterol. 2013;19:4702-17 pubmed publisher
Gwyer Findlay E, Villegas Mendez A, de Souza J, Inkson C, Shaw T, Saris C, et al. IL-27 receptor signaling regulates CD4+ T cell chemotactic responses during infection. J Immunol. 2013;190:4553-61 pubmed publisher
Kleinewietfeld M, Manzel A, Titze J, Kvakan H, Yosef N, Linker R, et al. Sodium chloride drives autoimmune disease by the induction of pathogenic TH17 cells. Nature. 2013;496:518-22 pubmed publisher
Chou J, Lin J, Brenot A, Kim J, Provot S, Werb Z. GATA3 suppresses metastasis and modulates the tumour microenvironment by regulating microRNA-29b expression. Nat Cell Biol. 2013;15:201-13 pubmed publisher
Peters J, Tjabringa G, Fasse E, de Oliveira V, Schalkwijk J, Koenen H, et al. Co-culture of healthy human keratinocytes and T-cells promotes keratinocyte chemokine production and ROR?t-positive IL-17 producing T-cell populations. J Dermatol Sci. 2013;69:44-53 pubmed publisher
Chen J, Huang P, Lee C, Chen P, Chen H. A bovine whey protein extract can induce the generation of regulatory T cells and shows potential to alleviate asthma symptoms in a murine asthma model. Br J Nutr. 2013;109:1813-20 pubmed publisher
Sawant D, Sehra S, Nguyen E, Jadhav R, Englert K, Shinnakasu R, et al. Bcl6 controls the Th2 inflammatory activity of regulatory T cells by repressing Gata3 function. J Immunol. 2012;189:4759-69 pubmed publisher
Blom L, Poulsen L. IL-1 family members IL-18 and IL-33 upregulate the inflammatory potential of differentiated human Th1 and Th2 cultures. J Immunol. 2012;189:4331-7 pubmed publisher
Gibson H, Mishra A, Chan D, Hake T, Porcu P, Wong H. Impaired proteasome function activates GATA3 in T cells and upregulates CTLA-4: relevance for Sézary syndrome. J Invest Dermatol. 2013;133:249-57 pubmed publisher
Shatynski K, Chen H, Kwon J, Williams M. Decreased STAT5 phosphorylation and GATA-3 expression in NOX2-deficient T cells: role in T helper development. Eur J Immunol. 2012;42:3202-11 pubmed publisher
Centuori S, Trad M, LaCasse C, Alizadeh D, Larmonier C, Hanke N, et al. Myeloid-derived suppressor cells from tumor-bearing mice impair TGF-?-induced differentiation of CD4+CD25+FoxP3+ Tregs from CD4+CD25-FoxP3- T cells. J Leukoc Biol. 2012;92:987-97 pubmed publisher
Grundström J, Linton L, Thunberg S, Forsslund H, Janczewska I, Befrits R, et al. Altered immunoregulatory profile during anti-tumour necrosis factor treatment of patients with inflammatory bowel disease. Clin Exp Immunol. 2012;169:137-47 pubmed publisher
Weiss R, Hessenberger M, Kitzmüller S, Bach D, Weinberger E, Krautgartner W, et al. Transcutaneous vaccination via laser microporation. J Control Release. 2012;162:391-9 pubmed publisher
Radulovic K, Manta C, Rossini V, Holzmann K, Kestler H, Wegenka U, et al. CD69 regulates type I IFN-induced tolerogenic signals to mucosal CD4 T cells that attenuate their colitogenic potential. J Immunol. 2012;188:2001-13 pubmed publisher
Hansmann L, Schmidl C, Kett J, Steger L, Andreesen R, Hoffmann P, et al. Dominant Th2 differentiation of human regulatory T cells upon loss of FOXP3 expression. J Immunol. 2012;188:1275-82 pubmed publisher
LaCasse C, Janikashvili N, Larmonier C, Alizadeh D, Hanke N, Kartchner J, et al. Th-1 lymphocytes induce dendritic cell tumor killing activity by an IFN-?-dependent mechanism. J Immunol. 2011;187:6310-7 pubmed publisher
Nakahira M, Nakanishi K. Requirement of GATA-binding protein 3 for II13 gene expression in IL-18-stimulated Th1 cells. Int Immunol. 2011;23:761-72 pubmed publisher
Blom L, Poulsen B, Jensen B, Hansen A, Poulsen L. IL-33 induces IL-9 production in human CD4+ T cells and basophils. PLoS ONE. 2011;6:e21695 pubmed publisher
Serre K, Bénézech C, Desanti G, Bobat S, Toellner K, Bird R, et al. Helios is associated with CD4 T cells differentiating to T helper 2 and follicular helper T cells in vivo independently of Foxp3 expression. PLoS ONE. 2011;6:e20731 pubmed publisher
Hebenstreit D, Fang M, Gu M, Charoensawan V, van Oudenaarden A, Teichmann S. RNA sequencing reveals two major classes of gene expression levels in metazoan cells. Mol Syst Biol. 2011;7:497 pubmed publisher
Lu Y, Malmhäll C, Sjöstrand M, Radinger M, O Neil S, Lotvall J, et al. Expansion of CD4(+) CD25(+) and CD25(-) T-Bet, GATA-3, Foxp3 and ROR?t cells in allergic inflammation, local lung distribution and chemokine gene expression. PLoS ONE. 2011;6:e19889 pubmed publisher
Hebenstreit D, Gu M, Haider S, Turner D, Lio P, Teichmann S. EpiChIP: gene-by-gene quantification of epigenetic modification levels. Nucleic Acids Res. 2011;39:e27 pubmed publisher
KEMP K, Levin S, Bryce P, Stein P. Lck mediates Th2 differentiation through effects on T-bet and GATA-3. J Immunol. 2010;184:4178-84 pubmed publisher
Mjösberg J, Berg G, Jenmalm M, Ernerudh J. FOXP3+ regulatory T cells and T helper 1, T helper 2, and T helper 17 cells in human early pregnancy decidua. Biol Reprod. 2010;82:698-705 pubmed publisher
Winer S, Chan Y, Paltser G, Truong D, Tsui H, Bahrami J, et al. Normalization of obesity-associated insulin resistance through immunotherapy. Nat Med. 2009;15:921-9 pubmed publisher
Guan H, Nagarkatti P, Nagarkatti M. Role of CD44 in the differentiation of Th1 and Th2 cells: CD44-deficiency enhances the development of Th2 effectors in response to sheep RBC and chicken ovalbumin. J Immunol. 2009;183:172-80 pubmed publisher
product information
Product Type :
Antibody
Product Name :
Gata-3 Monoclonal Antibody (TWAJ), eFluor 660, eBioscience
Catalog # :
50-9966-41
Quantity :
25 Tests
Price :
US 150
Clonality :
Monoclonal
Purity :
Affinity chromatography
Host :
Rat
Reactivity :
Human, Mouse, Porcine, Rhesus Monkey
Applications :
Flow Cytometry: 5 µL (0.125 µg)/test
Species :
Human, Mouse, Porcine, Rhesus Monkey
Clone :
TWAJ
Isotype :
IgG2b, kappa
Storage :
4° C, store in dark, DO NOT FREEZE!
Description :
The genes for all 4 subunits of the T-cell antigen receptor (alpha, beta, gamma and delta) are controlled by distinct enhancers and their enhancer-binding proteins. Marine and Winoto (1991) identified a common TCR regulatory element by demonstrating binding of the enhancer-binding protein GATA3 to the enhancer elements of all 4 TCR genes. GATA3 had been shown in the chicken to be an enhancer-binding protein containing a zinc finger domain. GATA3 mRNA was demonstrated by Northern blot analysis in T cells but not in B cells or macrophages. GATA3 is abundantly expressed in the T-lymphocyte lineage and is thought to participate in T-cell receptor gene activation through binding to enhancers. Labastie et al. (1994) cloned the human gene and the 5-prime end of the mouse gene. The human gene comprises 6 exons distributed over 17 kb of DNA. Its 2 zinc fingers are encoded by 2 separate exons highly conserved with those of GATA1, but no other structural homologies between the 2 genes could be found.
Format :
Liquid
Applications w/Dilutions :
Flow Cytometry: 5 µL (0.125 µg)/test
Aliases :
EGK_19445; GATA binding factor-3; GATA binding protein 3; GATA binding protein-3; GATA3; Gata-3; GATA-binding factor 3; GATA-binding protein 3; HDR; HDRS; jal; MGC2346; MGC5199; MGC5445; Trans-acting T-cell-specific transcription factor GATA-3; Transacting T-cell-specific transcription factor GATA-3
more info or order :
company information
Invitrogen
Thermo Fisher Scientific
81 Wyman Street
Waltham, MA USA 02451
https://www.thermofisher.com
800-678-5599
headquarters: USA