Alpha-Smooth Muscle Actin Monoclonal Antibody (1A4), eFluor™ 660, eBioscience | Invitrogen 50-9760-82 product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

Alpha-Smooth Muscle Actin Monoclonal Antibody (1A4), eFluor™ 660, eBioscience

catalog :

50-9760-82

quantity :

100 µg

price :

US 445.00

clonality :

monoclonal

host :

mouse

conjugate :

efluor 660

clone name :

1A4

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry, flow cytometry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

more info or order :

Invitrogen product webpage

citations: 38

Published Application/Species/Sample/Dilution	Reference
flow cytometry; mouse; loading ...; fig 2	Bi X, Du C, Wang X, Wang X, Han W, Wang Y, et al. Mitochondrial Damage-Induced Innate Immune Activation in Vascular Smooth Muscle Cells Promotes Chronic Kidney Disease-Associated Plaque Vulnerability. Adv Sci (Weinh). 2021;8:2002738 pubmed publisher
immunocytochemistry; human; loading ...; fig 2b	Ortiz Otero N, Marshall J, Lash B, King M. Chemotherapy-induced release of circulating-tumor cells into the bloodstream in collective migration units with cancer-associated fibroblasts in metastatic cancer patients. BMC Cancer. 2020;20:873 pubmed publisher
immunohistochemistry; mouse; 1:300; loading ...; fig s3g	Diéguez Hurtado R, Kato K, Giaimo B, Nieminen Kelhä M, Arf H, Ferrante F, et al. Loss of the transcription factor RBPJ induces disease-promoting properties in brain pericytes. Nat Commun. 2019;10:2817 pubmed publisher
immunohistochemistry - frozen section; mouse; 1:100; fig 2	Chen Q, Zhang H, Liu Y, Adams S, Eilken H, Stehling M, et al. Endothelial cells are progenitors of cardiac pericytes and vascular smooth muscle cells. Nat Commun. 2016;7:12422 pubmed publisher
	Jeong H, Di xe9 guez Hurtado R, Arf H, Song J, Park H, Kruse K, et al. Single-cell transcriptomics reveals functionally specialized vascular endothelium in brain. elife. 2022;11: pubmed publisher
	Liu R, Jia W, Wang Y, Hu C, Yu W, Huang Y, et al. Glymphatic System and Subsidiary Pathways Drive Nanoparticles Away from the Brain. Research (Wash D C). 2022;2022:9847612 pubmed publisher
	Hassel C, Gausser xe8 s B, Guzylack Piriou L, Foucras G. Ductal Macrophages Predominate in the Immune Landscape of the Lactating Mammary Gland. Front Immunol. 2021;12:754661 pubmed publisher
	Nagavally R, Sunilkumar S, Akhtar M, Trombetta L, Ford S. Chrysin Ameliorates Cyclosporine-A-Induced Renal Fibrosis by Inhibiting TGF-β1-Induced Epithelial-Mesenchymal Transition. Int J Mol Sci. 2021;22: pubmed publisher
	Wilm T, Tanton H, Mutter F, Foisor V, Middlehurst B, Ward K, et al. Restricted differentiative capacity of Wt1-expressing peritoneal mesothelium in postnatal and adult mice. Sci Rep. 2021;11:15940 pubmed publisher
	Morelli C, Castaldi L, Brown S, Streich L, Websdale A, Taberner F, et al. Identification of a population of peripheral sensory neurons that regulates blood pressure. Cell Rep. 2021;35:109191 pubmed publisher
	El Sherbiny M, Atef H, Eladl M, Mohamed A, El Shafey M, Ali H, et al. Leflunomide Induces Dose-Dependent Lung Injury in Mice via Stimulating Vimentin and NLRP3 Inflammasome Production. Front Pharmacol. 2021;12:631216 pubmed publisher
	Zhang L, He J, Wang J, Liu J, Chen Z, Deng B, et al. Knockout RAGE alleviates cardiac fibrosis through repressing endothelial-to-mesenchymal transition (EndMT) mediated by autophagy. Cell Death Dis. 2021;12:470 pubmed publisher
	Gilmore A, Flaherty S, Somasundaram V, Scheiblin D, Lockett S, Wink D, et al. An in vitro tumorigenesis model based on live-cell-generated oxygen and nutrient gradients. Commun Biol. 2021;4:477 pubmed publisher
	Prados A, Onder L, Cheng H, Mörbe U, Lütge M, Gil Cruz C, et al. Fibroblastic reticular cell lineage convergence in Peyer's patches governs intestinal immunity. Nat Immunol. 2021;22:510-519 pubmed publisher
	Xu Y, Wang N, Tan H, Li S, Zhang C, Feng Y. Gut-liver axis modulation of Panax notoginseng saponins in nonalcoholic fatty liver disease. Hepatol Int. 2021;15:350-365 pubmed publisher
	Panera N, Meroni M, Longo M, Crudele A, Valenti L, Bellacchio E, et al. The KLB rs17618244 gene variant is associated with fibrosing MAFLD by promoting hepatic stellate cell activation. EBioMedicine. 2021;65:103249 pubmed publisher
	Maia A, Gu Z, Koch A, Berdiel Acer M, Will R, Schlesner M, et al. IFNβ1 secreted by breast cancer cells undergoing chemotherapy reprograms stromal fibroblasts to support tumour growth after treatment. Mol Oncol. 2021;: pubmed publisher
	Huang S, Ziegler C, Austin J, Mannoun N, Vukovic M, Ordovas Montanes J, et al. Lymph nodes are innervated by a unique population of sensory neurons with immunomodulatory potential. Cell. 2021;184:441-459.e25 pubmed publisher
	Taniguchi R, Ono S, Isaji T, Gorecka J, Lee S, Matsubara Y, et al. A mouse model of stenosis distal to an arteriovenous fistula recapitulates human central venous stenosis. JVS Vasc Sci. 2020;1:109-122 pubmed publisher
	Lu S, Jolly A, Strand K, Dubner A, Mutryn M, Moulton K, et al. Smooth muscle-derived progenitor cell myofibroblast differentiation through KLF4 downregulation promotes arterial remodeling and fibrosis. JCI Insight. 2020;5: pubmed publisher
	Gilles G, McCulloch A, Brakebusch C, Herum K. Maintaining resting cardiac fibroblasts in vitro by disrupting mechanotransduction. PLoS ONE. 2020;15:e0241390 pubmed publisher
	Gorchs L, Ahmed S, Mayer C, Knauf A, Fernández Moro C, Svensson M, et al. The vitamin D analogue calcipotriol promotes an anti-tumorigenic phenotype of human pancreatic CAFs but reduces T cell mediated immunity. Sci Rep. 2020;10:17444 pubmed publisher
	Suess P, Smith S, Morrissey J. Platelet polyphosphate induces fibroblast chemotaxis and myofibroblast differentiation. J Thromb Haemost. 2020;18:3043-3052 pubmed publisher
	Xie B, Lu C, Chen C, Zhou J, Deng Z. miR-135a Alleviates Silica-Induced Pulmonary Fibrosis by Targeting NF-κB/Inflammatory Signaling Pathway. Mediators Inflamm. 2020;2020:1231243 pubmed publisher
	Gonzalez B, Perez Nevarez M, Mirza A, Perez M, Lin Y, Hsu C, et al. Physiologically Relevant Fluid-Induced Oscillatory Shear Stress Stimulation of Mesenchymal Stem Cells Enhances the Engineered Valve Matrix Phenotype. Front Cardiovasc Med. 2020;7:69 pubmed publisher
	Somarathna M, Isayeva Waldrop T, Al Balas A, Guo L, Lee T. A Novel Model of Balloon Angioplasty Injury in Rat Arteriovenous Fistula. J Vasc Res. 2020;57:223-235 pubmed publisher
	Polley A, Khanam R, Sengupta A, Chakraborty S. Asporin Reduces Adult Aortic Valve Interstitial Cell Mineralization Induced by Osteogenic Media and Wnt Signaling Manipulation In Vitro. Int J Cell Biol. 2020;2020:2045969 pubmed publisher
	Yazawa K, Nakamura F, Masukawa D, Sato S, Hiroshima Y, Yabushita Y, et al. Low Incidence of High-Grade Pancreatic Intraepithelial Neoplasia Lesions in a Crmp4 Gene-Deficient Mouse Model of Pancreatic Cancer. Transl Oncol. 2020;13:100746 pubmed publisher
	Wang T, Xie S, Luo R, Shi L, Bai P, Wang X, et al. Two novel TSC2 mutations in renal epithelioid angiomyolipoma sensitive to everolimus. Cancer Biol Ther. 2020;21:4-11 pubmed publisher
	Martinez L, Robila V, Clark N, Du W, Idowu M, Rutkowski M, et al. Regulatory T Cells Control the Switch From in situ to Invasive Breast Cancer. Front Immunol. 2019;10:1942 pubmed publisher
	Charbonnier B, Baradaran A, Sato D, Alghamdi O, Zhang Z, Zhang Y, et al. Material-Induced Venosome-Supported Bone Tubes. Adv Sci (Weinh). 2019;6:1900844 pubmed publisher
	Lin F, Zeng Z, Song Y, Li L, Wu Z, Zhang X, et al. YBX-1 mediated sorting of miR-133 into hypoxia/reoxygenation-induced EPC-derived exosomes to increase fibroblast angiogenesis and MEndoT. Stem Cell Res Ther. 2019;10:263 pubmed publisher
	Lin J, Izar B, Wang S, Yapp C, Mei S, Shah P, et al. Highly multiplexed immunofluorescence imaging of human tissues and tumors using t-CyCIF and conventional optical microscopes. elife. 2018;7: pubmed publisher
	Foo K, Lehtinen M, Leung C, Lian X, Xu J, Keung W, et al. Human ISL1+ Ventricular Progenitors Self-Assemble into an In Vivo Functional Heart Patch and Preserve Cardiac Function Post Infarction. Mol Ther. 2018;26:1644-1659 pubmed publisher
	Kaur H, Carvalho J, Looso M, Singh P, Chennupati R, Preussner J, et al. Single-cell profiling reveals heterogeneity and functional patterning of GPCR expression in the vascular system. Nat Commun. 2017;8:15700 pubmed publisher
	MercuŢ R, Ciurea M, Margaritescu C, Popescu S, Crăiţoiu M, Cotoi O, et al. Expression of p53, D2-40 and ?-smooth muscle actin in different histological subtypes of facial basal cell carcinoma. Rom J Morphol Embryol. 2014;55:263-72 pubmed
	Skalli O, Ropraz P, Trzeciak A, Benzonana G, Gillessen D, Gabbiani G. A monoclonal antibody against alpha-smooth muscle actin: a new probe for smooth muscle differentiation. J Cell Biol. 1986;103:2787-96 pubmed
	Roholl P, Elbers H, Prinsen I, Claessens J, van Unnik J. Distribution of actin isoforms in sarcomas: an immunohistochemical study. Hum Pathol. 1990;21:1269-74 pubmed

product information

Product Type :

Antibody

Product Name :

Alpha-Smooth Muscle Actin Monoclonal Antibody (1A4), eFluor™ 660, eBioscience

Catalog # :

50-9760-82

Quantity :

100 µg

Price :

US 445.00

Clonality :

Monoclonal

Purity :

Affinity chromatography

Host :

Mouse

Reactivity :

Human, Mouse, Rat

Applications :

Immunocytochemistry: 5 µg/mL, Immunohistochemistry (Frozen): 5 µg/mL, Immunohistochemistry (Paraffin): 2-5 µg/mL, Immunohistochemistry: 1:100, Western Blot: 1:1,000

Species :

Human, Mouse, Rat

Clone :

1A4

Isotype :

IgG2a, kappa

Storage :

4° C, store in dark, DO NOT FREEZE!

Description :

Smooth Muscle Actin belongs to the actin family of proteins, which are highly conserved proteins that play a role in cell motility, structure and integrity. Alpha, beta and gamma actin isoforms have been identified, with alpha actin being a major constituent of the contractile apparatus, while beta and gamma actins are involved in the regulation of cell motility. In particular, smooth muscle actin is an alpha actin that is found in skeletal muscle. Actin exists as a ubiquitous protein involved with filament formation that make up large portions of the cytoskeleton. Actin filaments interact with myosin to assist in muscle contraction as well as aiding in cell motility and cytokinesis. Smooth muscle actin is found on smooth muscle vessel walls, gut wall, myometrium, myoepithelial cells in breast and salivary glands. Defects in the smooth muscle actin gene cause aortic aneurysm familial thoracic type 6. Actin isoforms differ slightly in their N-terminus and the sequences of each are perfectly conserved in higher vertebrates. Alpha-smooth muscle actin is abundant in vascular and visceral smooth muscle cells. In addition, it has also been shown that smooth muscle actin appear in stress fibers of fibroblastic cells during pathological situations involving contractile phenomena such as wound healing and fibrocontractive diseases. Multiple alternatively spliced variants of smooth muscle actin have been identified.

Format :

Liquid

Applications w/Dilutions :

Immunocytochemistry: 5 µg/mL, Immunohistochemistry (Frozen): 5 µg/mL, Immunohistochemistry (Paraffin): 2-5 µg/mL, Immunohistochemistry: 1:100, Western Blot: 1:1,000

Aliases :

0610041G09Rik; AAT6; ACT-4; acta2; acta2.L; acta2.S; actin; Actin Alpha 2 Smooth Muscle Aorta; actin alpha 2, smooth muscle; Actin Vascular Smooth Muscle; actin, alpha 2, smooth muscle, aorta; actin, alpha 2, smooth muscle, aorta L homeolog; actin, alpha 2, smooth muscle, aorta S homeolog; actin, alpha, vascular smooth muscle; actin, aortic smooth muscle; Actin, aortic smooth muscle, intermediate form; ACTSA; ACTVS; alpha actin 2; Alpha Cardiac Actin; alpha smooth muscle actin; alpha-actin; Alpha-actin-2; alpha-cardiac actin; alphaSMA; alpha-SM-actin; alpha-smooth muscle actin; Aortic Smooth Muscle; ASMA; a-sma; cell growth-inhibiting gene 46 protein; GIG46; Growth Inhibiting Gene 46; hypothetical protein LOC515610; LOW QUALITY PROTEIN: actin, aortic smooth muscle; mymy5; SM actin; sma; SMactin; sm-actin; SMalphaA; smooth muscle; smooth muscle alpha-actin; vascular smooth muscle alpha-actin; wu:fb63d03; XELAEV_18034370mg

more info or order :

Invitrogen product webpage