AIRE Monoclonal Antibody (5H12), eFluor 660, eBioscience | Invitrogen 50-5934-80 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

AIRE Monoclonal Antibody (5H12), eFluor 660, eBioscience

catalog :

50-5934-80

quantity :

25 ug

price :

US 177.00

clonality :

monoclonal

host :

rat

conjugate :

efluor 660

clone name :

5H12

reactivity :

mouse

application :

immunohistochemistry, immunocytochemistry, flow cytometry, immunohistochemistry - frozen section

citations: 18

Reference
LUCAS B, White A, Klein F, Veiga Villauriz C, Handel A, Bacon A, et al. Embryonic keratin19+ progenitors generate multiple functionally distinct progeny to maintain epithelial diversity in the adult thymus medulla. Nat Commun. 2023;14:2066 pubmed publisher
Fujimori S, Ohigashi I, Abe H, Matsushita Y, Katagiri T, Taketo M, et al. Fine-tuning of β-catenin in mouse thymic epithelial cells is required for postnatal T-cell development. elife. 2022;11: pubmed publisher
Teng F, Tach xf3 Pi xf1 ot R, Sung B, Farber D, Worgall S, Hammad H, et al. ILC3s control airway inflammation by limiting T cell responses to allergens and microbes. Cell Rep. 2021;37:110051 pubmed publisher
Goldfarb Y, Givony T, Kadouri N, Dobe x161 J, Peligero Cruz C, Zalayat I, et al. Mechanistic dissection of dominant AIRE mutations in mouse models reveals AIRE autoregulation. J Exp Med. 2021;218: pubmed publisher
Zhang Q, Liang Z, Zhang J, Lei T, Dong X, Su H, et al. Sirt6 Regulates the Development of Medullary Thymic Epithelial Cells and Contributes to the Establishment of Central Immune Tolerance. Front Cell Dev Biol. 2021;9:655552 pubmed publisher
Farini A, Sitzia C, Villa C, Cassani B, Tripodi L, Legato M, et al. Defective dystrophic thymus determines degenerative changes in skeletal muscle. Nat Commun. 2021;12:2099 pubmed publisher
Swann J, Nusser A, Morimoto R, Nagakubo D, Boehm T. Retracing the evolutionary emergence of thymopoiesis. Sci Adv. 2020;6: pubmed publisher
Ohigashi I, Tanaka Y, Kondo K, Fujimori S, Kondo H, Palin A, et al. Trans-omics Impact of Thymoproteasome in Cortical Thymic Epithelial Cells. Cell Rep. 2019;29:2901-2916.e6 pubmed publisher
Wang H, Hogquist K. CCR7 defines a precursor for murine iNKT cells in thymus and periphery. elife. 2018;7: pubmed publisher
Liang Z, Zhang L, Su H, Luan R, Na N, Sun L, et al. MTOR signaling is essential for the development of thymic epithelial cells and the induction of central immune tolerance. Autophagy. 2017;: pubmed publisher
Ohigashi I, Ohte Y, Setoh K, Nakase H, Maekawa A, Kiyonari H, et al. A human PSMB11 variant affects thymoproteasome processing and CD8+ T cell production. JCI Insight. 2017;2: pubmed publisher
Takahashi T, Asano Y, Sugawara K, Yamashita T, Nakamura K, Saigusa R, et al. Epithelial Fli1 deficiency drives systemic autoimmunity and fibrosis: Possible roles in scleroderma. J Exp Med. 2017;214:1129-1151 pubmed publisher
Bansal K, Yoshida H, Benoist C, Mathis D. The transcriptional regulator Aire binds to and activates super-enhancers. Nat Immunol. 2017;18:263-273 pubmed publisher
McCarthy N, Cowan J, Nakamura K, Bacon A, Baik S, White A, et al. Osteoprotegerin-Mediated Homeostasis of Rank+ Thymic Epithelial Cells Does Not Limit Foxp3+ Regulatory T Cell Development. J Immunol. 2015;195:2675-82 pubmed publisher
Coder B, Wang H, Ruan L, Su D. Thymic involution perturbs negative selection leading to autoreactive T cells that induce chronic inflammation. J Immunol. 2015;194:5825-37 pubmed publisher
Waterfield M, Khan I, Cortez J, Fan U, Metzger T, Greer A, et al. The transcriptional regulator Aire coopts the repressive ATF7ip-MBD1 complex for the induction of immunotolerance. Nat Immunol. 2014;15:258-65 pubmed publisher
Irla M, Guenot J, Sealy G, Reith W, Imhof B, Serge A. Three-dimensional visualization of the mouse thymus organization in health and immunodeficiency. J Immunol. 2013;190:586-96 pubmed publisher
Rossi S, Kim M, Leibbrandt A, Parnell S, Jenkinson W, Glanville S, et al. RANK signals from CD4(+)3(-) inducer cells regulate development of Aire-expressing epithelial cells in the thymic medulla. J Exp Med. 2007;204:1267-72 pubmed

product information

Product Type :

Antibody

Product Name :

AIRE Monoclonal Antibody (5H12), eFluor 660, eBioscience

Catalog # :

50-5934-80

Quantity :

25 ug

Price :

US 177.00

Clonality :

Monoclonal

Purity :

Affinity chromatography

Host :

Rat

Reactivity :

Mouse

Applications :

Flow Cytometry: Assay-Dependent, Immunocytochemistry: 10 ug/mL, Immunohistochemistry (Frozen): 10 ug/mL

Species :

Mouse

Clone :

5H12

Isotype :

IgG2c, kappa

Storage :

4 C, store in dark, DO NOT FREEZE!

Description :

AIRE is a transcriptional regulator protein that binds to DNA as a dimer and tetramer, but not as a monomer, and may be involved in the regulation of gene expression. A nuclear protein, AIRE is a 545 amino acid protein with an HSR domain, two PHD type zinc fingers, and a SAND domain, and may be present in soluble high-molecular-weight complexes. It may form homodimer, homotetramer and also nuclear bodies and interacts with the transcriptional coactivator CBP and CREBBP. Three isoforms of AIRE are reported and one of them is predicted to be a candidate for nuclear-mediated decay (NMD). AIRE is involved in immune regulation and is known to mediate E3 ubiquitin ligase activity. Defects in AIRE are responsible for the rare autosomal-recessive systemic autoimmune disease termed autoimmune polyendocrinopathy-candidiasis-ectodermal dystrophy (APECED).

Format :

Liquid

Applications w/Dilutions :

Flow Cytometry: Assay-Dependent, Immunocytochemistry: 10 ug/mL, Immunohistochemistry (Frozen): 10 ug/mL

Aliases :

Aire; AIRE protein; AIRE1; APECED; APECED protein; APECED protein homolog; APS1; APSI; Autoimmune polyendocrinopathy candidiasis ectodermal dystrophy protein; autoimmune polyendocrinopathy candidiasis ectodermal dystrophy protein homolog; Autoimmune regulator; autoimmune regulator (autoimmune polyendocrinopathy candidiasis ectodermal dystrophy); autoimmune regulator AIRE1a; LOW QUALITY PROTEIN: autoimmune regulator; OTTHUMP00000109529; PGA1