SIGLEC H Monoclonal Antibody (eBio440c), eFluor™ 660, eBioscience | Invitrogen 50-0333-80 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

SIGLEC H Monoclonal Antibody (eBio440c), eFluor™ 660, eBioscience

catalog :

50-0333-80

quantity :

25 µg

price :

US 150

clonality :

monoclonal

host :

rat

conjugate :

efluor 660

clone name :

eBio440c

reactivity :

mouse

application :

flow cytometry

citations: 19

Reference
Jamali A, Hu K, Sendra V, Blanco T, Lopez M, Ortiz G, et al. Characterization of Resident Corneal Plasmacytoid Dendritic Cells and Their Pivotal Role in Herpes Simplex Keratitis. Cell Rep. 2020;32:108099 pubmed publisher
Hensel J, Khattar V, Ashton R, Ponnazhagan S. Characterization of immune cell subtypes in three commonly used mouse strains reveals gender and strain-specific variations. Lab Invest. 2019;99:93-106 pubmed publisher
Riepsaame J, van Oudenaren A, den Broeder B, van Ijcken W, Pothof J, Leenen P. MicroRNA-Mediated Down-Regulation of M-CSF Receptor Contributes to Maturation of Mouse Monocyte-Derived Dendritic Cells. Front Immunol. 2013;4:353 pubmed publisher
Liu H, Ramachandran I, Gabrilovich D. Regulation of plasmacytoid dendritic cell development in mice by aryl hydrocarbon receptor. Immunol Cell Biol. 2014;92:200-3 pubmed publisher
Maina V, Marrella V, Mantero S, Cassani B, Fontana E, Anselmo A, et al. Hypomorphic mutation in the RAG2 gene affects dendritic cell distribution and migration. J Leukoc Biol. 2013;94:1221-30 pubmed publisher
Baccarella A, Fontana M, Chen E, Kim C. Toll-like receptor 7 mediates early innate immune responses to malaria. Infect Immun. 2013;81:4431-42 pubmed publisher
Oderup C, Lajevic M, Butcher E. Canonical and noncanonical Wnt proteins program dendritic cell responses for tolerance. J Immunol. 2013;190:6126-34 pubmed publisher
Zeng R, Oderup C, Yuan R, Lee M, Habtezion A, Hadeiba H, et al. Retinoic acid regulates the development of a gut-homing precursor for intestinal dendritic cells. Mucosal Immunol. 2013;6:847-56 pubmed publisher
Kim C, Nelson C, Wilson E, Hou B, DeFranco A, Derisi J. Splenic red pulp macrophages produce type I interferons as early sentinels of malaria infection but are dispensable for control. PLoS ONE. 2012;7:e48126 pubmed publisher
Crother T, Ma J, Jupelli M, Chiba N, Chen S, Slepenkin A, et al. Plasmacytoid dendritic cells play a role for effective innate immune responses during Chlamydia pneumoniae infection in mice. PLoS ONE. 2012;7:e48655 pubmed publisher
Ho A, Prabhu N, Betts R, Ge M, Dai X, Hutchinson P, et al. Lung CD103+ dendritic cells efficiently transport influenza virus to the lymph node and load viral antigen onto MHC class I for presentation to CD8 T cells. J Immunol. 2011;187:6011-21 pubmed publisher
Davidson S, Kaiko G, Loh Z, Lalwani A, Zhang V, Spann K, et al. Plasmacytoid dendritic cells promote host defense against acute pneumovirus infection via the TLR7-MyD88-dependent signaling pathway. J Immunol. 2011;186:5938-48 pubmed publisher
Bao Y, Han Y, Chen Z, Xu S, Cao X. IFN-?-producing PDCA-1+ Siglec-H- B cells mediate innate immune defense by activating NK cells. Eur J Immunol. 2011;41:657-68 pubmed publisher
Charles E, Joshi S, Ash J, Fox B, Farris A, Bzik D, et al. CD4 T-cell suppression by cells from Toxoplasma gondii-infected retinas is mediated by surface protein PD-L1. Infect Immun. 2010;78:3484-92 pubmed publisher
Hermesh T, Moltedo B, Moran T, LOPEZ C. Antiviral instruction of bone marrow leukocytes during respiratory viral infections. Cell Host Microbe. 2010;7:343-53 pubmed publisher
Provoost S, Maes T, Willart M, Joos G, Lambrecht B, Tournoy K. Diesel exhaust particles stimulate adaptive immunity by acting on pulmonary dendritic cells. J Immunol. 2010;184:426-32 pubmed publisher
Blasius A, Giurisato E, Cella M, Schreiber R, Shaw A, Colonna M. Bone marrow stromal cell antigen 2 is a specific marker of type I IFN-producing cells in the naive mouse, but a promiscuous cell surface antigen following IFN stimulation. J Immunol. 2006;177:3260-5 pubmed
Blasius A, Cella M, Maldonado J, Takai T, Colonna M. Siglec-H is an IPC-specific receptor that modulates type I IFN secretion through DAP12. Blood. 2006;107:2474-6 pubmed
Blasius A, Vermi W, Krug A, Facchetti F, Cella M, Colonna M. A cell-surface molecule selectively expressed on murine natural interferon-producing cells that blocks secretion of interferon-alpha. Blood. 2004;103:4201-6 pubmed

product information

Product Type :

Antibody

Product Name :

SIGLEC H Monoclonal Antibody (eBio440c), eFluor™ 660, eBioscience

Catalog # :

50-0333-80

Quantity :

25 µg

Pricing :

US 150

Clonality :

Monoclonal

Purity :

Affinity chromatography

Host :

Rat

Reactivity :

Mouse

Applications :

Flow Cytometry: 0.25 µg/test

Species :

Mouse

Clone :

eBio440c

Isotype :

IgG2b, kappa

Storage :

4° C, store in dark, DO NOT FREEZE!

Description :

Siglec-H, a protein exclusively found on pDC (plasmacytoid dendritic cells) or type I IFN-producing cells (IPC) in the naive mouse. Mouse IPC are typically PDCA+, CD11c+, CD11b-, B220+, and Ly-6C+, and are quick to respond to viruses. Siglec-H is a transmembrane protein of the Ig superfamily that like CD33 have been shown to bind sialic acid but lacks the characteristic cytoplasmic ITIM domain (immunoreceptor tyrosine based inhibitory motif). To overcome the lack of a cytoplasmic domain, Siglec-H associates with DAP12 thereby allowing for signal transduction. The eBio440c antibody has been shown to inhibit pDC function (inhbits IFNalpha secretion in response to CpG). It has been observed that some mouse strains (such as SJL) have higher percentages of pDCs compared to C57BL/6.

Format :

Liquid

Applications w/Dilutions :

Flow Cytometry: 0.25 µg/test

Aliases :

6430529G09Rik; sialic acid binding Ig-like lectin H; Siglec; Siglech; Siglec-H; SIGLEC-like protein