mouse monoclonal (MANNES1A(7A12))
reactivity: human, mouse, rat
application: western blot, immunocytochemistry
citations: 8
reactivity: human, mouse, rat
application: western blot, immunocytochemistry
citations: 8
mouse monoclonal (6A)
reactivity: mouse, rat
application: western blot
reactivity: mouse, rat
application: western blot
Western blot analysis of CPG2 is shown using adult rat brain fractions and whole cell lysates from mock or CPG2 transfected HEK293T cells (kindly provided by Elly Nedivi's lab, MIT, Cambridge, MA) onto a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked with 5% Milk/TBST for at least 1 hour. Membranes were probed with a mouse monoclonal antibody recognizing CPG2 (Product # MA1-13082) at a dilution of 1:1000 overnight at 4°C on a rocking platform. Membranes were washed in TBS-0.1%Tween 20 and probed with a goat anti-mouse-HRP secondary antibody (Product # 31430) at a dilution of 1:20,000 for one hour. Membranes were washed and chemiluminescent detection performed using Pierce Super Signal West Dura (Product #34075).
quantity: 100 µg
price: US 410
to the supplier
mouse monoclonal (6A)
reactivity: mouse, rat
application: western blot
reactivity: mouse, rat
application: western blot
Western blot analysis of CPG2 is shown using adult rat brain fractions and whole cell lysates from mock or CPG2 transfected HEK293T cells (kindly provided by Elly Nedivi’s lab, MIT, Cambridge, MA) onto a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked with 5% Milk/TBST for at least 1 hour. Membranes were probed with a mouse monoclonal antibody recognizing CPG2 (Product # MA1-13082) at a dilution of 1:1000 overnight at 4C on a rocking platform. Membranes were washed in TBS-0.1%Tween 20 and probed with a goat anti-mouse-HRP secondary antibody (Product # 31430) at a dilution of 1:20,000 for one hour. Membranes were washed and chemiluminescent detection performed using Super Signal West Dura (Product #34075).
quantity: 20 µL
price: US 169
to the supplier
domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section
reactivity: human, mouse, rat
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section