This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Invitrogen
other brands :
NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience
product type :
antibody
product name :
H3K4me3 Polyclonal Antibody
catalog :
49-1005
quantity :
50 µg
price :
US 482
clonality :
polyclonal
host :
domestic rabbit
conjugate :
nonconjugated
reactivity :
human, mouse, zebrafish
application :
western blot, ELISA, immunocytochemistry, chromatin immunoprecipitation, ChIP-Seq
citations: 6
Published Application/Species/Sample/DilutionReference
  • chromatin immunoprecipitation; human; loading ...; fig 6c
Svoboda L, Bailey N, Van Noord R, Krook M, Harris A, Cramer C, et al. Tumorigenicity of Ewing sarcoma is critically dependent on the trithorax proteins MLL1 and menin. Oncotarget. 2017;8:458-471 pubmed publisher
  • ChIP-Seq; human; fig 1
Park Y, Nnamani M, Maziarz J, Wagner G. Cis-Regulatory Evolution of Forkhead Box O1 (FOXO1), a Terminal Selector Gene for Decidual Stromal Cell Identity. Mol Biol Evol. 2016;33:3161-3169 pubmed
  • chromatin immunoprecipitation; human; loading ...; fig 3c
Krook M, Hawkins A, Patel R, Lucas D, Van Noord R, Chugh R, et al. A bivalent promoter contributes to stress-induced plasticity of CXCR4 in Ewing sarcoma. Oncotarget. 2016;7:61775-61788 pubmed publisher
  • chromatin immunoprecipitation; human; loading ...; fig 3a
Sengupta D, Deb M, Rath S, Kar S, Parbin S, Pradhan N, et al. DNA methylation and not H3K4 trimethylation dictates the expression status of miR-152 gene which inhibits migration of breast cancer cells via DNMT1/CDH1 loop. Exp Cell Res. 2016;346:176-87 pubmed publisher
  • chromatin immunoprecipitation; human; fig 5
Deb M, Sengupta D, Kar S, Rath S, Roy S, Das G, et al. Epigenetic drift towards histone modifications regulates CAV1 gene expression in colon cancer. Gene. 2016;581:75-84 pubmed publisher
Parrish J, McCann T, Sechler M, Sobral L, Ren W, Jones K, et al. The Jumonji-domain histone demethylase inhibitor JIB-04 deregulates oncogenic programs and increases DNA damage in Ewing Sarcoma, resulting in impaired cell proliferation and survival, and reduced tumor growth. Oncotarget. 2018;9:33110-33123 pubmed publisher
product information
Product Type :
Antibody
Product Name :
H3K4me3 Polyclonal Antibody
Catalog # :
49-1005
Quantity :
50 µg
Price :
US 482
Clonality :
Polyclonal
Purity :
Affinity chromatography
Host :
Rabbit
Reactivity :
Fish, Human, Mouse, Plant, Zebrafish
Applications :
ELISA: 1:1,000, Immunocytochemistry: 1:200, Peptide array: 0.25 µg/mL, Western Blot: 1:1,000
Species :
Fish, Human, Mouse, Plant, Zebrafish
Isotype :
IgG
Storage :
-20° C, Avoid Freeze/Thaw Cycles
Description :
Histone H3 is one of the DNA-binding proteins found in the chromatin of all eukaryotic cells. H3 along with four core histone proteins binds to DNA forming the structure of the nucleosome. Histones play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. Post translationally, histones are modified in a variety of ways to either directly change the chromatin structure or allow for the binding of specific transcription factors. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of post-translational modification that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.
Immunogen :
Raised against the region of the histone H3 containing the trimethylated lysine 4 (or [K4me3]), using a KLH-conjugated synthetic peptide.
Format :
Liquid
Applications w/Dilutions :
ELISA: 1:1,000, Immunocytochemistry: 1:200, Peptide array: 0.25 µg/mL, Western Blot: 1:1,000
Aliases :
B0035.7; BUR5; CELE_T10C6.12; CG31613; CG31613-PA; CG33803; CG33806; CG33809; CG33812; CG33815; CG33818; CG33821; CG33824; CG33827; CG33830; CG33833; CG33836; CG33839; CG33842; CG33845; CG33848; CG33851; CG33854; CG33857; CG33860; CG33863; CG33866; Dmel CG31613; Dmel_CG31613; F07B7.10; F07B7.3; F08G2.2; F17E9.13; F35H10.1; F45F2.4; F54E12.5; F55G1.10; H02I12.7; H3; H3 histone; H3 histone family, member A; H3 histone family, member I; H3 histone family, member K; H3 histone family, member L; H3 histone family, member M; H3 histone, family 2; H3 histone, family 3A; H3 histone, family 3B; H3 histone, family 3B (H3.3B); H3 histone, family 3B.1; H3 histone, family 3C; H3 K4; H3.1-221; H3.1-291; H3.1-I; H3.2; H3.2-221; H3.2-614; H3.2-615; H3.2-616; h3.2a; H3.3A; H3.3B; H3.5; H3/A; H3/b; H3/d; H3/f; H3/i; H3/j; H3/k; H3/l; H3/M; H3/n; H3/o; H3-143; H3-291; H3-3A; H3-3b; h3-5; H3-53; H3-614; H3a; H3b; H3-B; H3C1; H3C10; H3C11; H3C12; H3C2; H3C3; H3C4; H3C6; H3C7; H3c8; H3f; H3-F; H3F1K; H3F2; H3F3; h3f3a; H3F3B; h3f3b.1; H3F3C; h3f3d; H3FA; H3FB; H3FC HIST1H3C; H3FD; H3FF; H3FH; H3FI; H3FJ; H3FK; H3FL; H3FM; H3FN; H3g; H3h; H3i; H3K4; H3Lys4me3; h3r; HHT1; HHT2; his-12; his-16; his-19; his-21; His3; his-3; His3:CG31613; His3:CG31613-PA; His3:CG33803; His3:CG33806; His3:CG33809; His3:CG33812; His3:CG33815; His3:CG33818; His3:CG33821; His3:CG33824; His3:CG33827; His3:CG33830; His3:CG33833; His3:CG33836; His3:CG33839; His3:CG33842; His3:CG33845; His3:CG33848; His3:CG33851; His3:CG33854; His3:CG33857; His3:CG33860; His3:CG33863; His3:CG33866; his-30; his-33; his-43; his-47; his-51; his-53; his-57; his-61; his-65; his-68; his-7; Hist1; Hist1h3a; HIST1H3B; Hist1h3c; Hist1h3d; HIST1H3E; Hist1h3f; hist1h3g; hist1h3g.L; HIST1H3H; Hist1h3i; HIST1H3J; hist2h3; HIST2H3A; Hist2h3b; HIST2H3C; Hist2h3c1; Hist2h3c2; Hist2h3c2-ps; Hist2h3ca1; Hist2h3ca2; HIST2H3D; histone 1, H3a; histone 1, H3b; histone 1, H3f; histone 1, H3h; histone 2, H3a; histone 2, H3c; histone 2, H3c2; histone 2, H3ca2; Histone 3; histone cluster 1, H3a; histone cluster 1, H3b; histone cluster 1, H3f; histone cluster 1, H3g protein L homeolog; histone cluster 1, H3h; histone cluster 2, H3a; histone cluster 2, H3c; histone cluster 2, H3c2; histone cluster 2, H3c2, pseudogene; histone gene complex 1; Histone H2A; Histone H3; histone H3.1; Histone H3.2; histone H3.3; Histone H3.3C; histone H3.5; Histone H3/a; Histone H3/b; Histone H3/c; Histone H3/d; Histone H3/f; Histone H3/h; Histone H3/i; Histone H3/j; Histone H3/k; histone H3/l; Histone H3/m; histone H3/o; Histone H3K4me3; histone variant H3.5; hypothetical protein LOC550262; K06C4.11; K06C4.3; M32461; methyl Histone 3; methyl Histone H3; N2749; PP781; SIN2; T10C6.12; T23D8.6; Trimethyl H3; Tri-methyl H3; Trimethyl Histone H3; Tri-methyl Histone H3; Tri-Methyl-H3 K4; Tri-Methyl-H3 Lys4; Tri-Methyl-Histone H3 K4; Tri-Methyl-Histone H3 Lys4; wu:fa25h06; wu:fa96g06; wu:fb07a08; wu:fb36f01; XELAEV_18028537mg; YBR010W; YBR0201; YNL031C; zgc:110292; zgc:174300; zgc:56193; zgc:56418; zgc:64222; zgc:86731; ZK131.10; ZK131.6
company information
Invitrogen
Thermo Fisher Scientific
81 Wyman Street
Waltham, MA USA 02451
https://www.thermofisher.com
800-678-5599
headquarters: USA