Invitrogen NRAS antibody

Pan Ras Monoclonal Antibody (Ras10)
Invitrogen
catalog: MA1-012X

mouse monoclonal (RAS10)
reactivity: human, mouse
application: western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section
citations: 17

Immunofluorescent analysis of Ras in HeLa Cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with a Ras monoclonal antibody (Product # MA1-012) at a dilution of 1:100 overnight at 4 C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody (Product # 35503). Ras staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Images were taken at 60X magnification.

Immunohistochemistry was performed on cancer biopsies of deparaffinized Human colon carcinoma tissue. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature and probed with a Ras monoclonal antibody (Product # MA1-012) at a dilution of 1:200 or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.

Western blot analysis of Ras was performed by loading 50ug of various whole cell lysate onto a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked with 5% Milk/TBST for at least 1 hour. Membranes were then probed with a mouse monoclonal antibody recognizing Ras (Product # MA1-012) at a dilution of 1:1000 overnight at 4°C on a rocking platform. Membranes were then washed in TBS-0.1%Tween 20 and probed with a goat anti-mouse-HRP secondary antibody (Product #31430) at a dilution of 1:20000 for at least one hour. Membranes were washed and chemiluminescent detection was performed using Pierce Super Signal West Pico(Product #34077).
quantity: 20 ug
price: US 177.00
to the supplier

Pan Ras Monoclonal Antibody (Ras10)
Invitrogen
catalog: MA1-012

quantity: 100 ug
price: US 502.00
to the supplier

N-Ras Polyclonal Antibody
Invitrogen
catalog: PA5-14833

domestic rabbit polyclonal
reactivity: human
application: western blot
citations: 5

quantity: 400 uL
price: US 474.00
to the supplier

N-Ras Polyclonal Antibody
Invitrogen
catalog: PA5-28861

domestic rabbit polyclonal
reactivity: human, mouse
application: western blot
citations: 2

Western Blot analysis of N-Ras using anti-N-Ras Polyclonal Antibody (PA5-28861), in Mouse Brain (50 ug of cell lysate) and at a dilution of 1:1000.
quantity: 100 uL
price: US 464.00
to the supplier

N-Ras Polyclonal Antibody
Invitrogen
catalog: PA5-34560

domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot
citations: 2

quantity: 100 ug
price: US 454.00
to the supplier

N-Ras Recombinant Rabbit Monoclonal Antibody (10H6L11)
Invitrogen
catalog: 703435

domestic rabbit recombinant (10H6L11)
reactivity: human, mouse
application: western blot, immunocytochemistry
citations: 1

quantity: 100 ug
price: US 520.00
to the supplier

N-Ras Polyclonal Antibody
Invitrogen
catalog: PA5-18888

domestic goat polyclonal
reactivity: human
application: western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

Western Blot staining of A431 cell lysate using PA5-18888 at a concentration of 0.01 ug/ml, the primary antibody incubation was 1 hour and the detection method was chemiluminescence.

Western blot of HEK293 overexpressing NRAS using PA5-18888, mock transfection as a control in first lane.