HSP70 Monoclonal Antibody (3A3)
Invitrogen
catalog: MA3-006

mouse monoclonal (3A3)
reactivity: Saccharomycetales, Meganyctiphanes norvegica, Pacific oyster, Japanese rice fish, snow fleas, Artemia franciscana, Asian green mussel, giant tiger prawn, Marenzelleria viridis, Chlamydomonas reinhardtii, red rock crab, lobsters, Eyprepocnemis plorans, common limpet, eastern oyster, roach minnow, crucian carp, holothurians, sea monkeys, human, mouse, rat, dogs, pigs , fruit fly , roundworm , rhesus macaque
application: western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunohistochemistry - paraffin section, blocking or activating experiments
citations: 98

Immunofluorescent analysis of Heat Shock Protein 70 using Heat Shock Protein 70 Monoclonal antibody (3A3) (Product# MA3-006) shows staining in HeLa cells. Heat Shock Protein 70 staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing Heat Shock Protein 70 (Product# MA3-006) at a dilution of 1:100-1:200 over night at 4 ?C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody (Product# 35552 for GAR, Product# 35503 for GAM). Images were taken at 60X magnification.

Western blot analysis of Heat Shock Protein 70 (Hsp70) was performed by loading 50ug of the indicated whole cell lysates and 15ul of PageRuler Prestained Protein Ladder (Product # 26616) onto a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked with 5% BSA/TBST for at least 1 hour. The membrane was probed with a Hsp70 monoclonal antibody (Product # MA3-006) at a dilution of 1:1000 overnight at 4°C on a rocking platform, washed in TBS-0.1%Tween 20, and probed with a goat anti-mouse IgG-HRP secondary antibody (Product # 31430) at a dilution of 1:20,000 for at least 1 hour. Chemiluminescent detection was performed using SuperSignal West Pico (Product # 34080).
quantity: 50 ug
price: US 538.00
to the supplier

HSP70 Monoclonal Antibody (5A5)
Invitrogen
catalog: MA3-007

mouse monoclonal (5A5)
reactivity: African green monkey, Pacific oyster, forest day mosquito, scallops, Lycaena tityrus, hamsters, California mussel, human, mouse, rat, dogs, fruit fly
application: western blot, ELISA, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section, blocking or activating experiments
citations: 54

Western blot analysis of Heat Shock Protein 70 (HSP70) was performed by loading 50µg of the indicated whole cell lysates and 15?l of PageRuler Prestained Protein Ladder (Product # 26616) onto a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked with 5% BSA/TBST for at least 1 hour. The membrane was probed with a HSP70 monoclonal antibody (Product # MA3-007) at a dilution of 1:1000 overnight at 4°C on a rocking platform, washed in TBS-0.1%Tween 20, and probed with a goat anti-mouse IgG HRP secondary antibody (Product # 31430) at a dilution of 1:20,000 for at least 1 hour. Chemiluminescent detection was performed using SuperSignal West Pico (Product # 34080).

Immunofluorescent analysis of Heat Shock Protein 70 using Heat Shock Protein 70 Monoclonal antibody (5A5) (Product# MA3-007) shows staining in HeLa cells. Heat Shock Protein 70 staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing Heat Shock Protein 70 (Product# MA3-007) at a dilution of 1:100-1:200 over night at 4 ?C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody (Product# 35552 for GAR, Product# 35503 for GAM). Images were taken at 60X magnification.

Immunohistochemistry was performed on cancer biopsies of deparaffinized Human prostate carcinoma tissue. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:100 with a mouse monoclonal antibody recognizing Heat Shock Protein 70 (MA3-007) or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.
quantity: 250 uL
price: US 538.00
to the supplier

HSP70 Monoclonal Antibody (2A4)
Invitrogen
catalog: MA3-008

mouse monoclonal (2A4)
reactivity: human, mouse, rat, fruit fly
application: western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunohistochemistry - paraffin section
citations: 14

Immunofluorescent analysis of Heat Shock Protein 70 (Hsp70) in HeLa Cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with a Heat Shock Protein 70 (Hsp70) monoclonal antibody (Product # MA3-008) at a dilution of 1:200 overnight at 4 C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody (Product # 35503). Heat Shock Protein 70 (Hsp70) staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Images were taken at 60X magnification.

Western blot analysis of Heat Shock Protein 70 (HSP70) was performed by loading 50ug of the indicated whole cell lysates and 15ul PageRuler Prestained Protein Ladder (Product # 26616) onto a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked with 5% BSA/TBST for at least 1 hour. The membrane was probed with a HSP70 monoclonal antibody (Product # MA3-008) at a dilution of 1:1000 overnight at 4°C on a rocking platform, washed in TBS-0.1%Tween-20, and probed with a goat anti-mouse IgM-HRP secondary antibody (Product # 31440) at a dilution of 1:20,000 for at least 1 hour. Chemiluminescent detection was performed using SuperSignal West Pico (Product # 34080).

Immunohistochemistry was performed on normal biopsies of deparaffinized Human breast tissue. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:200 with a mouse monoclonal antibody recognizing Heat Shock Protein 70 (MA3-008) or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.
quantity: 100 uL
price: US 538.00
to the supplier

HSP70 Monoclonal Antibody (7FB)
Invitrogen
catalog: MA5-27627

rat monoclonal (7FB)
reactivity: fruit fly
application: western blot, ELISA, immunocytochemistry

quantity: 100 ug
price: US 514.00
to the supplier

HSP70 Monoclonal Antibody (C92F3A-5), APC
Invitrogen
catalog: MA5-45090

mouse monoclonal (C92F3A-5)
reactivity: guinea pig, Chinese hamsters, human, mouse, rat, dogs, chicken, bovine, pigs , fruit fly , domestic sheep, domestic rabbit, roundworm
conjugate: APC
application: western blot, immunohistochemistry, immunocytochemistry, flow cytometry

quantity: 200 ug
price: US 470.00
to the supplier

HSP70 Monoclonal Antibody (C92F3A-5), FITC
Invitrogen
catalog: MA5-45091

mouse monoclonal (C92F3A-5)
reactivity: guinea pig, Chinese hamsters, human, mouse, rat, dogs, chicken, bovine, pigs , fruit fly , domestic sheep, domestic rabbit, roundworm
conjugate: FITC
application: western blot, immunohistochemistry, immunocytochemistry, flow cytometry

quantity: 200 ug
price: US 454.00
to the supplier

HSP70 Monoclonal Antibody (C92F3A-5), PerCP
Invitrogen
catalog: MA5-45092

mouse monoclonal (C92F3A-5)
reactivity: Chinese hamsters, guinea pig, human, mouse, rat, dogs, chicken, bovine, pigs , fruit fly , domestic sheep, domestic rabbit, roundworm
conjugate: PerCP
application: western blot, ELISA, immunohistochemistry, immunocytochemistry, flow cytometry

quantity: 200 ug
price: US 470.00
to the supplier

HSP70 Monoclonal Antibody (5A5), APC
Invitrogen
catalog: MA5-45208

mouse monoclonal (5A5)
reactivity: human, mouse, rat, chicken, fruit fly
conjugate: APC
application: western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation

quantity: 100 ug
price: US 410.00
to the supplier

HSP70 Monoclonal Antibody (5A5), FITC
Invitrogen
catalog: MA5-45209

mouse monoclonal (5A5)
reactivity: human, mouse, rat, chicken, fruit fly
conjugate: FITC
application: western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation

quantity: 100 ug
price: US 410.00
to the supplier

HSP70 Monoclonal Antibody (5A5), PerCP
Invitrogen
catalog: MA5-45210

mouse monoclonal (5A5)
reactivity: human, mouse, rat, chicken, fruit fly
conjugate: PerCP
application: western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation

quantity: 100 ug
price: US 410.00
to the supplier

HSP70 Monoclonal Antibody (3A3), APC
Invitrogen
catalog: MA5-45214

mouse monoclonal (3A3)
reactivity: human, mouse, rat, chicken, fruit fly
conjugate: APC
application: western blot, immunocytochemistry

quantity: 100 ug
price: US 410.00
to the supplier

HSP70 Monoclonal Antibody (3A3), FITC
Invitrogen
catalog: MA5-45215

mouse monoclonal (3A3)
reactivity: human, mouse, rat, chicken, fruit fly
conjugate: FITC
application: western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation

quantity: 100 ug
price: US 410.00
to the supplier

HSP70 Monoclonal Antibody (3A3), PerCP
Invitrogen
catalog: MA5-45216

mouse monoclonal (3A3)
reactivity: human, mouse, rat, chicken, fruit fly
conjugate: PerCP
application: western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation

quantity: 100 ug
price: US 410.00
to the supplier

HSP70 Monoclonal Antibody (7FB), APC
Invitrogen
catalog: MA5-45331

rat monoclonal (7FB)
reactivity: fruit fly
conjugate: APC
application: western blot, ELISA, immunocytochemistry

quantity: 100 ug
price: US 410.00
to the supplier

HSP70 Monoclonal Antibody (7FB), FITC
Invitrogen
catalog: MA5-45332

rat monoclonal (7FB)
reactivity: fruit fly
conjugate: FITC
application: western blot, ELISA, immunocytochemistry

quantity: 100 ug
price: US 410.00
to the supplier

HSP70 Monoclonal Antibody (7FB), PerCP
Invitrogen
catalog: MA5-45333

rat monoclonal (7FB)
reactivity: fruit fly
conjugate: PerCP
application: western blot, ELISA, immunocytochemistry

quantity: 100 ug
price: US 410.00
to the supplier

HSP70 Monoclonal Antibody (7FB), PE
Invitrogen
catalog: MA5-45334

rat monoclonal (7FB)
reactivity: fruit fly
conjugate: PE
application: western blot, ELISA, immunocytochemistry

quantity: 100 ug
price: US 410.00
to the supplier