PGP9.5 Monoclonal Antibody (BH7) | Invitrogen 480012 product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

PGP9.5 Monoclonal Antibody (BH7)

catalog :

480012

quantity :

100 µL

price :

US 476

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

BH7

reactivity :

human, mouse, rat, bovine, pigs

application :

western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

more info or order :

Invitrogen product webpage

citations: 11

Published Application/Species/Sample/Dilution	Reference
immunohistochemistry; rat; 1:1000; fig 3	Boric M, Jelicic Kadic A, Puljak L. Cutaneous expression of calcium/calmodulin-dependent protein kinase II in rats with type 1 and type 2 diabetes. J Chem Neuroanat. 2014;61-62:140-6 pubmed publisher
western blot; human	Zhao Z, Wu L, Xiong R, Wang L, Zhang B, Wang C, et al. MicroRNA-922 promotes tau phosphorylation by downregulating ubiquitin carboxy-terminal hydrolase L1 (UCHL1) expression in the pathogenesis of Alzheimer's disease. Neuroscience. 2014;275:232-7 pubmed publisher
immunohistochemistry - paraffin section; rat; 1:500	Filipović N, Zuvan L, Masek T, Tokalić R, Grkovic I. Gender and gonadectomy influence on neurons in superior cervical ganglia of sexually mature rats. Neurosci Lett. 2014;563:55-60 pubmed publisher
immunohistochemistry; rat; 1:1000; fig 4	Jelicic Kadic A, Boric M, Vidak M, Ferhatovic L, Puljak L. Changes in epidermal thickness and cutaneous innervation during maturation in long-term diabetes. J Tissue Viability. 2014;23:7-12 pubmed publisher
immunohistochemistry; rat; 1:1000; fig 5	Boric M, Skopljanac I, Ferhatovic L, Jelicic Kadic A, Banozic A, Puljak L. Reduced epidermal thickness, nerve degeneration and increased pain-related behavior in rats with diabetes type 1 and 2. J Chem Neuroanat. 2013;53:33-40 pubmed publisher
western blot; human; 1:5000; fig 1	Zhang Y, Zhou L, Rouge L, Phillips A, Lam C, Liu P, et al. Conformational stabilization of ubiquitin yields potent and selective inhibitors of USP7. Nat Chem Biol. 2013;9:51-8 pubmed publisher
immunohistochemistry - paraffin section; human	Lee J, Ladd A, Hagert E. Immunofluorescent triple-staining technique to identify sensory nerve endings in human thumb ligaments. Cells Tissues Organs. 2012;195:456-64 pubmed publisher
	Jurić M, Zeitler J, Vukojevic K, Bocina I, Grobe M, Kretzschmar G, et al. Expression of Connexins 37, 43 and 45 in Developing Human Spinal Cord and Ganglia. Int J Mol Sci. 2020;21: pubmed publisher
	Urlic M, Urlić I, Urlić H, Masek T, Benzon B, Vitlov Uljevic M, et al. Effects of Different n6/n3 PUFAs Dietary Ratio on Cardiac Diabetic Neuropathy. Nutrients. 2020;12: pubmed publisher
	Hewings D, Heideker J, Ma T, AhYoung A, El Oualid F, Amore A, et al. Reactive-site-centric chemoproteomics identifies a distinct class of deubiquitinase enzymes. Nat Commun. 2018;9:1162 pubmed publisher
	Bheda A, Gullapalli A, Caplow M, Pagano J, Shackelford J. Ubiquitin editing enzyme UCH L1 and microtubule dynamics: implication in mitosis. Cell Cycle. 2010;9:980-94 pubmed

product information

Product Type :

Antibody

Product Name :

PGP9.5 Monoclonal Antibody (BH7)

Catalog # :

480012

Quantity :

100 µL

Price :

US 476

Clonality :

Monoclonal

Purity :

Protein G

Host :

Mouse

Reactivity :

Bovine, Human, Mouse, Porcine, Rat

Applications :

Immunocytochemistry: 1:500, Immunohistochemistry: 1:500-1:5,000, Western Blot: 1:5,000

Species :

Bovine, Human, Mouse, Porcine, Rat

Clone :

BH7

Isotype :

IgG1

Storage :

-20° C, Avoid Freeze/Thaw Cycles

Description :

PGP9.5 (Protein gene product 9.5, UCH-L1, PARK5) is a neuron specific protein, structurally and immunologically distinct from neuron specific enolase. PGP9.5 has a molecular weight of 27 kDa and was first identified by high resolution two dimensional PAGE. PGP9.5 is a member of ubiquitin carboxyl-terminal hydrolase family 1 (peptidase family C12) with a ubiquitin carboxyl-terminal hydrolase domain. PGP9.5 is well known for having ubiquitin hydrolase and ligase activities that hydrolyzes small C-terminal adducts of ubiquitin to generate ubiquitin monomers. PGP9.5 is present in neurons and nerve fibers at all levels of the central and peripheral nervous system, in neuroendocrine cells, in segments of the renal tubules, in spermatogonia and Leydig cells of the testis, in ova and in some cells of both the pregnant and non-pregnant corpus luteum. Over expression of PGP9.5 leads to non-small cell lung cancer while decreased expression leads to Huntington disease and Alzheimer disease. Since PGP9.5 is present in cellular inclusions, it can be a useful as a neuronal marker and in the studies of neurodegenerative disorders such as with Parkinson disease.

Immunogen :

Recombinant full length human UCHL1 purified from E. coli.

Format :

Liquid

Applications w/Dilutions :

Immunocytochemistry: 1:500, Immunohistochemistry: 1:500-1:5,000, Western Blot: 1:5,000

Aliases :

AW822034; C88048; epididymis luminal protein 117; epididymis secretory protein Li 53; gad; gracile axonal dystrophy; HEL-117; HEL-S-53; MGC133697 protein; NDGOA; neuron cytoplasmic protein 9.5; OTTHUMP00000218137; OTTHUMP00000218139; OTTHUMP00000218140; OTTHUMP00000218141; PARK5; PGP 9.5; PGP9.5; PGP95; protein gene product 9.5; R75593; ubiquitin carboxyl-terminal esterase; ubiquitin carboxyl-terminal esterase L1 (ubiquitin thiolesterase); ubiquitin carboxyl-terminal hydrolase isozyme L1; ubiquitin carboxyl-terminal hydrolase L1; ubiquitin carboxy-terminal hydrolase L1; ubiquitin C-terminal hydrolase; ubiquitin C-terminal hydrolase 1; ubiquitin C-terminal hydrolase L1; ubiquitin thioesterase L1; ubiquitin thiolesterase; ubiquitin thiolesterase L1; UCHL; UCHL1; UCH-L1; UCHL-1

more info or order :

Invitrogen product webpage