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company name :
Invitrogen
other brands :
NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience
product type :
antibody
product name :
CD45.1 Monoclonal Antibody (A20), eFluor™ 450, eBioscience
catalog :
48-0453-82
quantity :
100 µg
price :
US 309.00
clonality :
monoclonal
host :
mouse
conjugate :
eFluor 450
clone name :
A20
reactivity :
mouse
application :
flow cytometry
more info or order :
citations: 56
Published Application/Species/Sample/DilutionReference
  • flow cytometry; mouse; loading ...; fig 7c, 7d, 7e
Lu L, Li T, Feng X, Liu Z, Liu Y, Chao T, et al. Excessive immunosuppression by regulatory T cells antagonizes T cell response to schistosome infection in PD-1-deficient mice. PLoS Pathog. 2022;18:e1010596 pubmed publisher
  • flow cytometry; mouse; 1:100; loading ...; fig 3s1a
Glassman C, Su L, Majri Morrison S, Winkelmann H, Mo F, Li P, et al. Calibration of cell-intrinsic interleukin-2 response thresholds guides design of a regulatory T cell biased agonist. elife. 2021;10: pubmed publisher
  • flow cytometry; mouse; loading ...; fig 6d
Kunimoto H, Meydan C, Nazir A, Whitfield J, Shank K, Rapaport F, et al. Cooperative Epigenetic Remodeling by TET2 Loss and NRAS Mutation Drives Myeloid Transformation and MEK Inhibitor Sensitivity. Cancer Cell. 2018;33:44-59.e8 pubmed publisher
  • flow cytometry; mouse; fig 1
Ramo K, Sugamura K, Craige S, Keaney J, Davis R. Suppression of ischemia in arterial occlusive disease by JNK-promoted native collateral artery development. elife. 2016;5: pubmed publisher
Tondini E, Reintjens N, Castello G, Arakelian T, Isendoorn M, Camps M, et al. Lipid A analog CRX-527 conjugated to synthetic peptides enhances vaccination efficacy and tumor control. NPJ Vaccines. 2022;7:64 pubmed publisher
Zotos D, Quast I, Li Wai Suen C, McKenzie C, Robinson M, Kan A, et al. The concerted change in the distribution of cell cycle phases and zone composition in germinal centers is regulated by IL-21. Nat Commun. 2021;12:7160 pubmed publisher
Young I, Wu B, Andricovich J, Chuang S, Li R, Tzatsos A, et al. Differentiation of fetal hematopoietic stem cells requires ARID4B to restrict autocrine KITLG/KIT-Src signaling. Cell Rep. 2021;37:110036 pubmed publisher
Lefebvre M, Surette F, Anthony S, Vijay R, Jensen I, Pewe L, et al. Expeditious recruitment of circulating memory CD8 T cells to the liver facilitates control of malaria. Cell Rep. 2021;37:109956 pubmed publisher
Sano T, Kageyama T, Fang V, Kedmi R, Martinez C, Talbot J, et al. Redundant cytokine requirement for intestinal microbiota-induced Th17 cell differentiation in draining lymph nodes. Cell Rep. 2021;36:109608 pubmed publisher
Seydoux E, Wan Y, Feng J, Wall A, Aljedani S, Homad L, et al. Development of a VRC01-class germline targeting immunogen derived from anti-idiotypic antibodies. Cell Rep. 2021;35:109084 pubmed publisher
Lv K, Gong C, Antony C, Han X, Ren J, Donaghy R, et al. HectD1 controls hematopoietic stem cell regeneration by coordinating ribosome assembly and protein synthesis. Cell Stem Cell. 2021;28:1275-1290.e9 pubmed publisher
Rivas M, Meydan C, Chin C, Challman M, Kim D, Bhinder B, et al. Smc3 dosage regulates B cell transit through germinal centers and restricts their malignant transformation. Nat Immunol. 2021;22:240-253 pubmed publisher
Kawashima K, Isogawa M, Onishi M, Baudi I, Saito S, Nakajima A, et al. Restoration of type I interferon signaling in intrahepatically primed CD8+ T cells promotes functional differentiation. JCI Insight. 2021;6: pubmed publisher
Khouili S, Cook E, Hernández García E, Martínez López M, Conde Garrosa R, Iborra S. SHP-1 Regulates Antigen Cross-Presentation and Is Exploited by Leishmania to Evade Immunity. Cell Rep. 2020;33:108468 pubmed publisher
Still K, Batista S, O Brien C, Oyesola O, Früh S, Webb L, et al. Astrocytes promote a protective immune response to brain Toxoplasma gondii infection via IL-33-ST2 signaling. PLoS Pathog. 2020;16:e1009027 pubmed publisher
Flosbach M, Oberle S, Scherer S, Zecha J, von Hoesslin M, Wiede F, et al. PTPN2 Deficiency Enhances Programmed T Cell Expansion and Survival Capacity of Activated T Cells. Cell Rep. 2020;32:107957 pubmed publisher
Milner J, Toma C, He Z, Kurd N, Nguyen Q, McDonald B, et al. Heterogenous Populations of Tissue-Resident CD8+ T Cells Are Generated in Response to Infection and Malignancy. Immunity. 2020;52:808-824.e7 pubmed publisher
Rana A, de Almeida F, Paico Montero H, Gonzales Carazas M, Bortoluci K, Sad S, et al. RIPK3 and Caspase-1/11 Are Necessary for Optimal Antigen-Specific CD8 T Cell Response Elicited by Genetically Modified Listeria monocytogenes. Front Immunol. 2020;11:536 pubmed publisher
Keenan C, Iannarella N, Naselli G, Bediaga N, Johanson T, Harrison L, et al. Extreme disruption of heterochromatin is required for accelerated hematopoietic aging. Blood. 2020;135:2049-2058 pubmed publisher
Nadafi R, Gago de Graça C, Keuning E, Koning J, de Kivit S, Konijn T, et al. Lymph Node Stromal Cells Generate Antigen-Specific Regulatory T Cells and Control Autoreactive T and B Cell Responses. Cell Rep. 2020;30:4110-4123.e4 pubmed publisher
Wang K, Yan Z, Zhang S, Bartholdy B, Eaves C, Bouhassira E. Clonal origin in normal adults of all blood lineages and circulating hematopoietic stem cells. Exp Hematol. 2020;83:25-34.e2 pubmed publisher
Sidwell T, Liao Y, Garnham A, Vasanthakumar A, Gloury R, Blume J, et al. Attenuation of TCR-induced transcription by Bach2 controls regulatory T cell differentiation and homeostasis. Nat Commun. 2020;11:252 pubmed publisher
Ciecko A, Foda B, Barr J, Ramanathan S, Atkinson M, Serreze D, et al. Interleukin-27 Is Essential for Type 1 Diabetes Development and Sjögren Syndrome-like Inflammation. Cell Rep. 2019;29:3073-3086.e5 pubmed publisher
Zhang X, Feng J, Chen S, Yang H, Dong Z. Synergized regulation of NK cell education by NKG2A and specific Ly49 family members. Nat Commun. 2019;10:5010 pubmed publisher
Zhang H, Carnevale G, Polese B, Simard M, Thurairajah B, Khan N, et al. CD109 Restrains Activation of Cutaneous IL-17-Producing γδ T Cells by Commensal Microbiota. Cell Rep. 2019;29:391-405.e5 pubmed publisher
Viny A, Bowman R, Liu Y, Lavallée V, Eisman S, Xiao W, et al. Cohesin Members Stag1 and Stag2 Display Distinct Roles in Chromatin Accessibility and Topological Control of HSC Self-Renewal and Differentiation. Cell Stem Cell. 2019;25:682-696.e8 pubmed publisher
Anderson C, Reilly E, Lee A, Brossay L. Qa-1-Restricted CD8+ T Cells Can Compensate for the Absence of Conventional T Cells during Viral Infection. Cell Rep. 2019;27:537-548.e5 pubmed publisher
Wang K, Guzman A, Yan Z, Zhang S, Hu M, Hamaneh M, et al. Ultra-High-Frequency Reprogramming of Individual Long-Term Hematopoietic Stem Cells Yields Low Somatic Variant Induced Pluripotent Stem Cells. Cell Rep. 2019;26:2580-2592.e7 pubmed publisher
Martínez López M, Iborra S, Conde Garrosa R, Mastrangelo A, Danne C, Mann E, et al. Microbiota Sensing by Mincle-Syk Axis in Dendritic Cells Regulates Interleukin-17 and -22 Production and Promotes Intestinal Barrier Integrity. Immunity. 2019;50:446-461.e9 pubmed publisher
Beneke V, Küster F, Neehus A, Hesse C, Lopez Rodriguez E, Haake K, et al. An immune cell spray (ICS) formulation allows for the delivery of functional monocyte/macrophages. Sci Rep. 2018;8:16281 pubmed publisher
Dudeck J, Froebel J, Kotrba J, Lehmann C, Dudziak D, Speier S, et al. Engulfment of mast cell secretory granules on skin inflammation boosts dendritic cell migration and priming efficiency. J Allergy Clin Immunol. 2019;143:1849-1864.e4 pubmed publisher
Schmidt K, Zhang Q, Tasdogan A, Petzold A, Dahl A, Arneth B, et al. The H3K4 methyltransferase Setd1b is essential for hematopoietic stem and progenitor cell homeostasis in mice. elife. 2018;7: pubmed publisher
Gaya M, Barral P, Burbage M, Aggarwal S, Montaner B, Warren Navia A, et al. Initiation of Antiviral B Cell Immunity Relies on Innate Signals from Spatially Positioned NKT Cells. Cell. 2018;172:517-533.e20 pubmed publisher
Baratin M, Simon L, Jorquera A, Ghigo C, Dembele D, Nowak J, et al. T Cell Zone Resident Macrophages Silently Dispose of Apoptotic Cells in the Lymph Node. Immunity. 2017;47:349-362.e5 pubmed publisher
Miao T, Symonds A, Singh R, Symonds J, Ogbe A, Omodho B, et al. Egr2 and 3 control adaptive immune responses by temporally uncoupling expansion from T cell differentiation. J Exp Med. 2017;214:1787-1808 pubmed publisher
Lehmann C, Baranska A, Heidkamp G, Heger L, Neubert K, Lühr J, et al. DC subset-specific induction of T cell responses upon antigen uptake via Fc? receptors in vivo. J Exp Med. 2017;214:1509-1528 pubmed publisher
Schmitt M, Sitte S, Voehringer D. CD4 T Helper Cells Instruct Lymphopenia-Induced Memory-Like CD8 T Cells for Control of Acute LCMV Infection. Front Immunol. 2016;7:622 pubmed publisher
Damgaard R, Walker J, Marco Casanova P, Morgan N, Titheradge H, Elliott P, et al. The Deubiquitinase OTULIN Is an Essential Negative Regulator of Inflammation and Autoimmunity. Cell. 2016;166:1215-1230.e20 pubmed publisher
Mucci A, Kunkiel J, Suzuki T, Brennig S, Glage S, Kühnel M, et al. Murine iPSC-Derived Macrophages as a Tool for Disease Modeling of Hereditary Pulmonary Alveolar Proteinosis due to Csf2rb Deficiency. Stem Cell Reports. 2016;7:292-305 pubmed publisher
Pietras E, Mirantes Barbeito C, Fong S, Loeffler D, Kovtonyuk L, Zhang S, et al. Chronic interleukin-1 exposure drives haematopoietic stem cells towards precocious myeloid differentiation at the expense of self-renewal. Nat Cell Biol. 2016;18:607-18 pubmed publisher
Ghosh M, Shapiro L. In vitro Ag Cross-presentation and in vivo Ag Cross-presentation by Dendritic Cells in the Mouse. Bio Protoc. 2012;2:e305 pubmed
Wu L, Li N, Zhang M, Xue S, Cassady K, Lin Q, et al. MHC-mismatched mixed chimerism augments thymic regulatory T-cell production and prevents relapse of EAE in mice. Proc Natl Acad Sci U S A. 2015;112:15994-9 pubmed publisher
Xue J, Sharma V, Hsieh M, Chawla A, Murali R, Pandol S, et al. Alternatively activated macrophages promote pancreatic fibrosis in chronic pancreatitis. Nat Commun. 2015;6:7158 pubmed publisher
Brestoff J, Kim B, Saenz S, Stine R, Monticelli L, Sonnenberg G, et al. Group 2 innate lymphoid cells promote beiging of white adipose tissue and limit obesity. Nature. 2015;519:242-6 pubmed publisher
Xia P, Wang S, Huang G, Zhu P, Li M, Ye B, et al. WASH is required for the differentiation commitment of hematopoietic stem cells in a c-Myc-dependent manner. J Exp Med. 2014;211:2119-34 pubmed publisher
Cowan J, McCarthy N, Parnell S, White A, Bacon A, Serge A, et al. Differential requirement for CCR4 and CCR7 during the development of innate and adaptive ??T cells in the adult thymus. J Immunol. 2014;193:1204-12 pubmed publisher
Haldar M, Kohyama M, So A, Kc W, Wu X, Briseño C, et al. Heme-mediated SPI-C induction promotes monocyte differentiation into iron-recycling macrophages. Cell. 2014;156:1223-1234 pubmed publisher
Agudo J, Ruzo A, Tung N, Salmon H, Leboeuf M, Hashimoto D, et al. The miR-126-VEGFR2 axis controls the innate response to pathogen-associated nucleic acids. Nat Immunol. 2014;15:54-62 pubmed publisher
Barao I, Wright P, Sungur C, Anderson S, Redelman D, Murphy W. Differential expression of the Ly49G(B6), but not the Ly49G(BALB), receptor isoform during natural killer cell reconstitution after hematopoietic stem cell transplantation. Biol Blood Marrow Transplant. 2013;19:1446-52 pubmed publisher
Grosselin J, Sii Felice K, Payen E, Chretien S, Tronik Le Roux D, Leboulch P. Arrayed lentiviral barcoding for quantification analysis of hematopoietic dynamics. Stem Cells. 2013;31:2162-71 pubmed publisher
Cowan J, Parnell S, Nakamura K, Caamano J, Lane P, Jenkinson E, et al. The thymic medulla is required for Foxp3+ regulatory but not conventional CD4+ thymocyte development. J Exp Med. 2013;210:675-81 pubmed publisher
Reis B, Rogoz A, Costa Pinto F, Taniuchi I, Mucida D. Mutual expression of the transcription factors Runx3 and ThPOK regulates intestinal CD4? T cell immunity. Nat Immunol. 2013;14:271-80 pubmed publisher
Ma D, Suthar M, Kasahara S, Gale M, Clark E. CD22 is required for protection against West Nile virus Infection. J Virol. 2013;87:3361-75 pubmed publisher
Wong S, Walker J, Jolin H, Drynan L, Hams E, Camelo A, et al. Transcription factor ROR? is critical for nuocyte development. Nat Immunol. 2012;13:229-36 pubmed publisher
Mizutani M, Pino P, Saederup N, Charo I, Ransohoff R, Cardona A. The fractalkine receptor but not CCR2 is present on microglia from embryonic development throughout adulthood. J Immunol. 2012;188:29-36 pubmed publisher
Monticelli L, Sonnenberg G, Abt M, Alenghat T, Ziegler C, Doering T, et al. Innate lymphoid cells promote lung-tissue homeostasis after infection with influenza virus. Nat Immunol. 2011;12:1045-54 pubmed publisher
product information
Product Type :
Antibody
Product Name :
CD45.1 Monoclonal Antibody (A20), eFluor™ 450, eBioscience
Catalog # :
48-0453-82
Quantity :
100 µg
Price :
US 309.00
Clonality :
Monoclonal
Purity :
Affinity chromatography
Host :
Mouse
Reactivity :
Mouse
Applications :
Flow Cytometry: 0.5 µg/test
Species :
Mouse
Clone :
A20
Isotype :
IgG2a, kappa
Storage :
4° C, store in dark, DO NOT FREEZE!
Description :
CD45 (LCA, leukocyte common antigen) is a receptor-type protein tyrosine phosphatase ubiquitously expressed in all nucleated hematopoietic cells, comprising approximately 10% of all surface proteins in lymphocytes. CD45 glycoprotein is crucial in lymphocyte development and antigen signaling, serving as an important regulator of Src-family kinases. CD45 protein exists as multiple isoforms as a result of alternative splicing; these isoforms differ in their extracellular domains, whereas they share identical transmembrane and cytoplasmic domains. These CD45 isoforms differ in their ability to translocate into the glycosphingolipid-enriched membrane domains and their expression depends on cell type and physiological state of the cell. Besides the role in immunoreceptor signaling, CD45 is important in promoting cell survival by modulating integrin-mediated signal transduction pathway and is also involved in DNA fragmentation during apoptosis. CD45RA is an isoform of the CD45 complex and has restricted expression between different subtypes of lymphoid cells.
Format :
Liquid
Applications w/Dilutions :
Flow Cytometry: 0.5 µg/test
Aliases :
B220; Cd45; CD45 antigen; CD45R; GP180; Lca; L-CA; Leukocyte common antigen; loc; LY5; Ly-5; Lymphocyte antigen 5; lymphocyte common antigen; Lyt-4; protein tyrosine phosphatase, receptor type C; protein tyrosine phosphatase, receptor type, C; protein tyrosine phosphatase, receptor type, c polypeptide; PTPRC; Receptor-type tyrosine-protein phosphatase C; RT7; T200; T200 glycoprotein; T200 leukocyte common antigen
more info or order :
company information
Invitrogen
Thermo Fisher Scientific
81 Wyman Street
Waltham, MA USA 02451
https://www.thermofisher.com
800-678-5599
headquarters: USA