CD20 Monoclonal Antibody (2H7), eFluor 450, eBioscience™ | Invitrogen 48-0209-42 product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

CD20 Monoclonal Antibody (2H7), eFluor 450, eBioscience™

catalog :

48-0209-42

quantity :

100 Tests

price :

US 309.00

clonality :

monoclonal

host :

mouse

conjugate :

eFluor 450

clone name :

2H7

reactivity :

human, rhesus macaque

application :

flow cytometry

more info or order :

Invitrogen product webpage

citations: 13

Published Application/Species/Sample/Dilution	Reference
flow cytometry; human; fig s1b	Stephenson S, Care M, Fan I, Zougman A, Westhead D, Doody G, et al. Growth Factor-like Gene Regulation Is Separable from Survival and Maturation in Antibody-Secreting Cells. J Immunol. 2019;202:1287-1300 pubmed publisher
flow cytometry; rhesus macaque; 1:32	Sestak K, Feely S, Fey B, Dufour J, Hargitt E, Alvarez X, et al. Experimental inoculation of juvenile rhesus macaques with primate enteric caliciviruses. PLoS ONE. 2012;7:e37973 pubmed publisher
	Colonna L, Peterson C, Schell J, Carlson J, Tkachev V, Brown M, et al. Evidence for persistence of the SHIV reservoir early after MHC haploidentical hematopoietic stem cell transplantation. Nat Commun. 2018;9:4438 pubmed publisher
	Heger L, Balk S, Lühr J, Heidkamp G, Lehmann C, Hatscher L, et al. CLEC10A Is a Specific Marker for Human CD1c+ Dendritic Cells and Enhances Their Toll-Like Receptor 7/8-Induced Cytokine Secretion. Front Immunol. 2018;9:744 pubmed publisher
	Somasundaram R, Zhang G, Fukunaga Kalabis M, Perego M, Krepler C, Xu X, et al. Tumor-associated B-cells induce tumor heterogeneity and therapy resistance. Nat Commun. 2017;8:607 pubmed publisher
	Arumugakani G, Stephenson S, Newton D, Rawstron A, Emery P, Doody G, et al. Early Emergence of CD19-Negative Human Antibody-Secreting Cells at the Plasmablast to Plasma Cell Transition. J Immunol. 2017;198:4618-4628 pubmed publisher
	Chen X, Li J, Zeng L, Zhang X, Lu X, Zuo M, et al. Sexual Differences in Cell Loss during the Post-Hatch Development of Song Control Nuclei in the Bengalese Finch. PLoS ONE. 2015;10:e0125802 pubmed publisher
	Bachanova V, Frankel A, Cao Q, Lewis D, Grzywacz B, Verneris M, et al. Phase I study of a bispecific ligand-directed toxin targeting CD22 and CD19 (DT2219) for refractory B-cell malignancies. Clin Cancer Res. 2015;21:1267-72 pubmed publisher
	Bergkvist K, Nyegaard M, Bøgsted M, Schmitz A, Bødker J, Rasmussen S, et al. Validation and implementation of a method for microarray gene expression profiling of minor B-cell subpopulations in man. BMC Immunol. 2014;15:3 pubmed publisher
	Jensen K, Brusletto B, Aass H, Olstad O, Kierulf P, Gautvik K. Transcriptional profiling of mRNAs and microRNAs in human bone marrow precursor B cells identifies subset- and age-specific variations. PLoS ONE. 2013;8:e70721 pubmed publisher
	Chicaybam L, Sodré A, Curzio B, Bonamino M. An efficient low cost method for gene transfer to T lymphocytes. PLoS ONE. 2013;8:e60298 pubmed publisher
	Cocco M, Stephenson S, Care M, Newton D, Barnes N, Davison A, et al. In vitro generation of long-lived human plasma cells. J Immunol. 2012;189:5773-85 pubmed publisher
	Kling H, Shipley T, Norris K. Alterations in peripheral blood B-cell populations in SHIV89.6P-infected macaques (Macacca fascicularis). Comp Med. 2011;61:269-77 pubmed

product information

Product Type :

Antibody

Product Name :

CD20 Monoclonal Antibody (2H7), eFluor 450, eBioscience™

Catalog # :

48-0209-42

Quantity :

100 Tests

Price :

US 309.00

Clonality :

Monoclonal

Purity :

Affinity chromatography

Host :

Mouse

Reactivity :

Human

Applications :

Flow Cytometry: 5 µL (0.5 µg)/test

Species :

Human

Clone :

2H7

Isotype :

IgG2b, kappa

Storage :

4° C, store in dark, DO NOT FREEZE!

Description :

CD20 is a non-glycosylated surface phosphoprotein that has a molecular weight range of 33-37 kDa depending on the degree of phosphorylation. CD20 is expressed on mature and most malignant B cells, in a subpopulation of T lymphocytes and follicular dendritic cells. CD20 expression on B cells is synchronous with the expression of surface IgM and it regulates transmembrane calcium conductance, cell cycle progression and B-cell proliferation. CD20 is also associated with lipid rafts, but the intensity of this association depends on extracellular triggering, employing CD20 conformational change, and/or BCR (B cell antigen receptor) aggregation. After the receptor ligation, BCR and CD20 colocalize and then rapidly dissociate before BCR endocytosis, whereas CD20 remains at the cell surface. CD20 serves as a useful target for antibody-mediated therapeutic depletion of B cells, as it is expressed at high levels on most B-cell malignancies, but does not become internalized or shed from the plasma membrane following monoclonal antibody treatment. Diseases associated with CD20 dysfunction include Ms4a1-related common variable immune deficiency.

Format :

Liquid

Applications w/Dilutions :

Flow Cytometry: 5 µL (0.5 µg)/test

Aliases :

AA960661; B1; B-cell differentiation antigen Ly-44; B-lymphocyte antigen CD20; B-lymphocyte cell-surface antigen B1; B-lymphocyte surface antigen B1; Bp35; Cd20; CD20 antigen; CD20 cell surface protein; CD20 receptor; CVID5; EGK_06167; LEU-16; Leukocyte surface antigen Leu-16; Ly-44; Lymphocyte antigen 44; membrane spanning 4-domains A1; membrane-spanning 4-domains subfamily A member 1; membrane-spanning 4-domains, subfamily A, member 1; membrane-spanning 4-domains, subfamily A, member 2; MGC3969; MS4A1; MS4A2; S7

more info or order :

Invitrogen product webpage